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GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown root systems.
- Source :
-
ELife [Elife] 2015 Aug 19; Vol. 4. Date of Electronic Publication: 2015 Aug 19. - Publication Year :
- 2015
-
Abstract
- Root systems develop different root types that individually sense cues from their local environment and integrate this information with systemic signals. This complex multi-dimensional amalgam of inputs enables continuous adjustment of root growth rates, direction, and metabolic activity that define a dynamic physical network. Current methods for analyzing root biology balance physiological relevance with imaging capability. To bridge this divide, we developed an integrated-imaging system called Growth and Luminescence Observatory for Roots (GLO-Roots) that uses luminescence-based reporters to enable studies of root architecture and gene expression patterns in soil-grown, light-shielded roots. We have developed image analysis algorithms that allow the spatial integration of soil properties, gene expression, and root system architecture traits. We propose GLO-Roots as a system that has great utility in presenting environmental stimuli to roots in ways that evoke natural adaptive responses and in providing tools for studying the multi-dimensional nature of such processes.
Details
- Language :
- English
- ISSN :
- 2050-084X
- Volume :
- 4
- Database :
- MEDLINE
- Journal :
- ELife
- Publication Type :
- Academic Journal
- Accession number :
- 26287479
- Full Text :
- https://doi.org/10.7554/eLife.07597