1. [i]Dickeya solani[/i] sp nov., a pectinolytic plant-pathogenic bacterium isolated from potato ([i]Solanum tuberosum[/i])
- Author
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Brion Duffy, E.H. Nijhuis, Marta Potrykus, Leighton Pritchard, Ian K. Toth, John G. Elphinstone, Malgorzata Waleron, Ilse Cleenwerck, Valérie Hélias, Valentin Pflüger, Shula Manulis, Linda Garlant, Malgorzata J. Kowalewska, Leah Tsror, G. S. Saddler, Paul De Vos, Joël F. Pothier, Minna Pirhonen, Ewa Lojkowska, Neil Parkinson, Jan M. van der Wolf, Plant Research International, Wageningen University and Research [Wageningen] (WUR), Science and Advice for Scottish Agriculture, Food and Environment Research Agency, The James Hutton Institute, Intercollegiate Faculty of Biotechnology, Medical University of Gdańsk-University of Gdańsk (UG), BCCM/LMG Bacteria Collection, Universiteit Gent = Ghent University [Belgium] (UGENT), Department of Applied Biology, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institute of Natural Resource Sciences, Zürich University of Applied Sciences (ZHAW), Plant Protection Division, Agroscope, Mabritec AG, Volcani Center, Department of Plant Pathology and Weed Research, Agricultural Research Organization, Universiteit Gent = Ghent University (UGENT), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Wageningen University and Research Centre [Wageningen] (WUR), Medical University of Gdańsk-University of Gdańsk, Ghent University [Belgium] (UGENT), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA)
- Subjects
DNA, Bacterial ,relatedness ,Indoles ,Pectobacterium ,Biovar ,chrysanthemi ,[SDV]Life Sciences [q-bio] ,Molecular Sequence Data ,Pectobacterium chrysanthemi ,Dickeya ,clonality ,genus ,Microbiology ,strains ,Intergenic region ,Enterobacteriaceae ,RNA, Ribosomal, 16S ,dnaX ,Israel ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Plant Diseases ,Solanum tuberosum ,2. Zero hunger ,mallei ,biology ,clade ,pseudomallei ,Fatty Acids ,Nucleic Acid Hybridization ,General Medicine ,570: Biologie ,biology.organism_classification ,Dickeya dadantii ,Bacterial Typing Techniques ,Europe ,Genes, Bacterial ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Pectins ,PRI BIOINT Ecological Interactions ,identification ,Dickeya solani ,Multilocus Sequence Typing - Abstract
Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya , previously the Pectobacterium chrysanthemi complex ( Erwinia chrysanthemi ), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi , showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya . The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB, rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA–DNA hybridization studies, showed that although related to Dickeya dadantii , these isolates represent a novel species within the genus Dickeya , for which the name Dickeya solani sp. nov. (type strain IPO 2222T = LMG25993T = NCPPB4479T) is proposed.
- Published
- 2014
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