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[i]Dickeya solani[/i] sp nov., a pectinolytic plant-pathogenic bacterium isolated from potato ([i]Solanum tuberosum[/i])
[i]Dickeya solani[/i] sp nov., a pectinolytic plant-pathogenic bacterium isolated from potato ([i]Solanum tuberosum[/i])
- Source :
- International Journal of Systematic and Evolutionary Microbiology, International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2014, 64 (3), pp.768-774. ⟨10.1099/ijs.0.052944-0⟩, International Journal of Systematic and Evolutionary Microbiology, 2014, 64 (3), pp.768-774. ⟨10.1099/ijs.0.052944-0⟩, International Journal of Systematic and Evolutionary Microbiology, 64(Pt. 3), 768-774, International Journal of Systematic and Evolutionary Microbiology 64 (2014) Pt. 3
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya , previously the Pectobacterium chrysanthemi complex ( Erwinia chrysanthemi ), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi , showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya . The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB, rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA–DNA hybridization studies, showed that although related to Dickeya dadantii , these isolates represent a novel species within the genus Dickeya , for which the name Dickeya solani sp. nov. (type strain IPO 2222T = LMG25993T = NCPPB4479T) is proposed.
- Subjects :
- DNA, Bacterial
relatedness
Indoles
Pectobacterium
Biovar
chrysanthemi
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Pectobacterium chrysanthemi
Dickeya
clonality
genus
Microbiology
strains
Intergenic region
Enterobacteriaceae
RNA, Ribosomal, 16S
dnaX
Israel
Phylogeny
Ecology, Evolution, Behavior and Systematics
Plant Diseases
Solanum tuberosum
2. Zero hunger
mallei
biology
clade
pseudomallei
Fatty Acids
Nucleic Acid Hybridization
General Medicine
570: Biologie
biology.organism_classification
Dickeya dadantii
Bacterial Typing Techniques
Europe
Genes, Bacterial
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Pectins
PRI BIOINT Ecological Interactions
identification
Dickeya solani
Multilocus Sequence Typing
Subjects
Details
- Language :
- English
- ISSN :
- 14665026 and 14665034
- Database :
- OpenAIRE
- Journal :
- International Journal of Systematic and Evolutionary Microbiology, International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2014, 64 (3), pp.768-774. ⟨10.1099/ijs.0.052944-0⟩, International Journal of Systematic and Evolutionary Microbiology, 2014, 64 (3), pp.768-774. ⟨10.1099/ijs.0.052944-0⟩, International Journal of Systematic and Evolutionary Microbiology, 64(Pt. 3), 768-774, International Journal of Systematic and Evolutionary Microbiology 64 (2014) Pt. 3
- Accession number :
- edsair.doi.dedup.....c9bbd1b6055f489226c595a3504fd6ac
- Full Text :
- https://doi.org/10.1099/ijs.0.052944-0⟩