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[i]Dickeya solani[/i] sp nov., a pectinolytic plant-pathogenic bacterium isolated from potato ([i]Solanum tuberosum[/i])

[i]Dickeya solani[/i] sp nov., a pectinolytic plant-pathogenic bacterium isolated from potato ([i]Solanum tuberosum[/i])

Authors :
Brion Duffy
E.H. Nijhuis
Marta Potrykus
Leighton Pritchard
Ian K. Toth
John G. Elphinstone
Malgorzata Waleron
Ilse Cleenwerck
Valérie Hélias
Valentin Pflüger
Shula Manulis
Linda Garlant
Malgorzata J. Kowalewska
Leah Tsror
G. S. Saddler
Paul De Vos
Joël F. Pothier
Minna Pirhonen
Ewa Lojkowska
Neil Parkinson
Jan M. van der Wolf
Plant Research International
Wageningen University and Research [Wageningen] (WUR)
Science and Advice for Scottish Agriculture
Food and Environment Research Agency
The James Hutton Institute
Intercollegiate Faculty of Biotechnology
Medical University of Gdańsk-University of Gdańsk (UG)
BCCM/LMG Bacteria Collection
Universiteit Gent = Ghent University [Belgium] (UGENT)
Department of Applied Biology
Institut de Génétique, Environnement et Protection des Plantes (IGEPP)
Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1)
Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST
Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Institute of Natural Resource Sciences
Zürich University of Applied Sciences (ZHAW)
Plant Protection Division
Agroscope
Mabritec AG
Volcani Center, Department of Plant Pathology and Weed Research
Agricultural Research Organization
Universiteit Gent = Ghent University (UGENT)
Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST
Wageningen University and Research Centre [Wageningen] (WUR)
Medical University of Gdańsk-University of Gdańsk
Ghent University [Belgium] (UGENT)
AGROCAMPUS OUEST-Université de Rennes 1 (UR1)
Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA)
Source :
International Journal of Systematic and Evolutionary Microbiology, International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2014, 64 (3), pp.768-774. ⟨10.1099/ijs.0.052944-0⟩, International Journal of Systematic and Evolutionary Microbiology, 2014, 64 (3), pp.768-774. ⟨10.1099/ijs.0.052944-0⟩, International Journal of Systematic and Evolutionary Microbiology, 64(Pt. 3), 768-774, International Journal of Systematic and Evolutionary Microbiology 64 (2014) Pt. 3
Publication Year :
2014
Publisher :
HAL CCSD, 2014.

Abstract

Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya , previously the Pectobacterium chrysanthemi complex ( Erwinia chrysanthemi ), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi , showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya . The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB, rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA–DNA hybridization studies, showed that although related to Dickeya dadantii , these isolates represent a novel species within the genus Dickeya , for which the name Dickeya solani sp. nov. (type strain IPO 2222T = LMG25993T = NCPPB4479T) is proposed.

Details

Language :
English
ISSN :
14665026 and 14665034
Database :
OpenAIRE
Journal :
International Journal of Systematic and Evolutionary Microbiology, International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2014, 64 (3), pp.768-774. ⟨10.1099/ijs.0.052944-0⟩, International Journal of Systematic and Evolutionary Microbiology, 2014, 64 (3), pp.768-774. ⟨10.1099/ijs.0.052944-0⟩, International Journal of Systematic and Evolutionary Microbiology, 64(Pt. 3), 768-774, International Journal of Systematic and Evolutionary Microbiology 64 (2014) Pt. 3
Accession number :
edsair.doi.dedup.....c9bbd1b6055f489226c595a3504fd6ac
Full Text :
https://doi.org/10.1099/ijs.0.052944-0⟩