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1. Quantum Frequency Mixing using an NV Diamond Microscope

Author

Karlson, Samuel J., Kehayias, Pauli, Schloss, Jennifer M., Maccabe, Andrew C., Phillips, David F., Wang, Guoqing, Cappellaro, Paola, and Braje, Danielle A.

Subjects
Physics - Instrumentation and Detectors, Physics - Optics, Quantum Physics
Abstract

Wide-field magnetic microscopy using nitrogen-vacancy (NV) centers in diamond can yield high-quality magnetic images of DC and AC magnetic fields. The unique combination of micron-scale spatial resolution of scalar or vector fields at room temperature and parallel camera readout make this an appealing technique for applications in biology, geology, condensed-matter physics, and electronics. However, while NV magnetic microscopy has achieved great success in these areas, historically the accessible frequency range has been limited. In this paper, we overcome this limitation by implementing the recently developed technique of quantum frequency mixing. With this approach, we generate wide-field magnetic images of test structures driven by alternating currents up to 70 MHz, well outside the reach of DC and Rabi magnetometry methods. With further improvements, this approach could find utility in hyperspectral imaging for electronics power spectrum analysis, electronics diagnostics and troubleshooting, and quantum computing hardware validation., Comment: 8 pages main text, 3 pages supplement

Published
2024

2. Longevity Studies of CSC Prototypes Operating with Ar+CO$_{2}$ Gas Mixture and Different Fractions of CF$_{4}$

Author

Barberis, Emanuela, Begovic, Nebojsa, Haubrich, Nicholas, Ignatenko, Mikhail, Korytov, Andrey, Kukral, Ota, Kuznetsova, Ekaterina, Lanaro, Armando, MacCabe, Andrew, Milenovic, Predrag, Milovanovic, Dubravka, Mitselmakher, Guenakh, Radulovic, Aleksandra, Rajcic, Boris, Rosenzweig, Jake, Wang, Bingran, Wang, Jian, Wisecarver, Andrew, Wood, Darien, and Yeager, Emma

Subjects
Physics - Instrumentation and Detectors
Abstract

Studies of Cathode Strip Chamber longevity, comparing Ar+CO2 gas mixtures with fractions of 5%, 2%, and 0% CF4, were performed using several small cathode strip prototype chambers. In each trial, a localized source of radiation was used to irradiate up to an accumulated charge of about 300 mC/cm. Additionally, longevity of a uniformly irradiated prototype operating with 2% CF4 was studied at the CERN Gamma Irradiation Facility GIF++. Post-hoc analysis of the chamber electrodes using spectroscopy techniques was also done.

Published
2024

3. A Solid-State Microwave Magnetometer with Picotesla-Level Sensitivity

Author

Alsid, Scott T., Schloss, Jennifer M., Steinecker, Matthew H., Barry, John F., Maccabe, Andrew C., Wang, Guoqing, Cappellaro, Paola, and Braje, Danielle A.

Subjects
Quantum Physics, Condensed Matter - Mesoscale and Nanoscale Physics, Physics - Applied Physics
Abstract

Quantum sensing of low-frequency magnetic fields using nitrogen-vacancy (NV) center ensembles has been demonstrated in multiple experiments with sensitivities as low as $\sim$1 pT/$\sqrt{\text{Hz}}$. To date, however, demonstrations of high-frequency magnetometry in the GHz regime with NV diamond are orders of magnitude less sensitive, above the nT/$\sqrt{\text{Hz}}$ level. Here we adapt for microwave frequencies techniques that have enabled high-performance, low-frequency quantum sensors. Using a custom-grown NV-enriched diamond combined with a noise cancellation scheme designed for high-frequency sensing, we demonstrate a Rabi-sequence-based magnetometer able to detect microwave fields near 2.87 GHz with a record sensitivity of 3.4 pT/$\sqrt{\textrm{Hz}}$. We demonstrate both amplitude and phase sensing and project tunability over a 300 MHz frequency range. This result increases the viability of NV ensembles to serve as microwave circuitry imagers and near-field probes of antennas., Comment: 7 pages, 4 figures

Published
2022

4. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

de Vries, Ronald P, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Uchima, Cristiane Akemi, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna A, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo C, Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, dos Santos, Renato Augusto Corrêa, Damásio, André Ricardo de Lima, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María Laura, Henrissat, Bernard, Hildén, Kristiina S, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen L, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio F, MacCabe, Andrew, Mäkelä, Miia R, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos P, Mulé, Giuseppina, Ngan, Chew Yee, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean Paul, Overkamp, Karin M, Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J, Ram, Arthur FJ, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego Mauricio, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S, Samson, Rob A, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio M, Sun, Hui, Susca, Antonia, Todd, Richard B, Tsang, Adrian, Unkles, Shiela E, van de Wiele, Nathalie, van Rossen-Uffink, Diana, Oliveira, Juliana Velasco de Castro, Vesth, Tammi C, Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miaomiao, and Andersen, Mikael R

Subjects
Human Genome, Genetics, Biotechnology, Emerging Infectious Diseases, Infectious Diseases, Adaptation, Biological, Aspergillus, Biodiversity, Biomass, Carbon, Computational Biology, Cytochrome P-450 Enzyme System, DNA Methylation, Fungal Proteins, Gene Expression Regulation, Fungal, Gene Regulatory Networks, Genome, Fungal, Genomics, Humans, Metabolic Networks and Pathways, Molecular Sequence Annotation, Multigene Family, Oxidoreductases, Phylogeny, Plants, Secondary Metabolism, Signal Transduction, Stress, Physiological, Genome sequencing, Comparative genomics, Fungal biology, Environmental Sciences, Biological Sciences, Information and Computing Sciences, Bioinformatics
Abstract

BackgroundThe fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus.ResultsWe have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli.ConclusionsMany aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.

Published
2017

5. Longevity studies of CSC prototypes operating with Ar+CO$$_{2}$$ gas mixture and different fractions of CF$$_{4}$$

Author

Barberis, Emanuela, primary, Begovic, Nebojsa, additional, Haubrich, Nicholas, additional, Ignatenko, Mikhail, additional, Korytov, Andrey, additional, Kukral, Ota, additional, Kuznetsova, Ekaterina, additional, Lanaro, Armando, additional, MacCabe, Andrew, additional, Milenovic, Predrag, additional, Milovanovic, Dubravka, additional, Mitselmakher, Guenakh, additional, Radulovic, Aleksandra, additional, Rajcic, Boris, additional, Rosenzweig, Jake, additional, Wang, Bingran, additional, Wang, Jian, additional, Wisecarver, Andrew, additional, Wood, Darien, additional, and Yeager, Emma, additional

Published
2024
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14. Identification of the genes encoding the catalytic steps corresponding to LRA4 (l‐2‐keto‐3‐deoxyrhamnonate aldolase) and l‐lactaldehyde dehydrogenase in Aspergillus nidulans: evidence for involvement of the loci AN9425/lraD and AN0544/aldA in the l‐rhamnose catabolic pathway

Author

MacCabe, Andrew P., Sanmartín, Gemma, Orejas, Margarita, Ministerio de Economía y Competitividad (España), and Generalitat Valenciana

Subjects
Filamentous fungi, Catabolism, L‐rhamnose, Aspergillus nidulans
Abstract

This is the peer reviewed version of the following article: MacCabe, A., Sanmartín, G. and Orejas, M. (2021), Identification of the genes encoding the catalytic steps corresponding to LRA4 (l‐2‐keto‐3‐deoxyrhamnonate aldolase) and l‐lactaldehyde dehydrogenase in Aspergillus nidulans: evidence for involvement of the loci AN9425/lraD and AN0544/aldA in the l‐rhamnose catabolic pathway. Environ Microbiol. https://doi.org/10.1111/1462-2920.15439, which has been published in final form at https://doi.org/10.1111/1462-2920.15439. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions., l‐rhamnose is found in nature mainly as a component of structural plant polysaccharides and can be used as a carbon source by certain microorganisms. Catabolism of this sugar in bacteria, archaea and fungi occurs by two routes involving either phosphorylated or non‐phosphorylated intermediates. Unlike the corresponding pathway in yeasts, the metabolic details of the non‐phosphorylated pathway in filamentous fungi are not fully defined. The first three genes (lraA, lraB and lraC) of the non‐phosphorylated pathway in Aspergillus nidulans have recently been studied revealing dependence on lraA function for growth on l‐rhamnose and α‐l‐rhamnosidase production. In the present work, two genes encoding the subsequent steps catalysed by l‐2‐keto‐3‐deoxyrhamnonate (l‐KDR) aldolase (AN9425) and l‐lactaldehyde dehydrogenase (AN0554) are identified. Loss‐of‐function mutations cause adverse growth effects on l‐rhamnose. Akin to genes lraA‐C and those encoding rhamnosidases (rhaA, rhaE), their expression is induced on l‐rhamnose via the transcriptional activator RhaR. Interestingly, the aldolase belongs to the ftablamily of bacterial l‐KDR aldolases (PF03328/COG3836) and not that of yeasts (PF00701/COG0329). In addition, AN0554 corresponds to the previously characterized aldA gene (encodes aldehyde dehydrogenase involved in ethanol utilization) thus revealing a previously unknown role for this gene in the catabolism of l‐rhamnose., This work was supported by the Spanish Ministerio de Economía y Competitividad/FEDER (grant number AGL2015‐6631‐C2‐2‐R) ‐ which also supported G.S. ‐ and the Generalitat Valenciana‐Conselleria d'Educació PROMETEO/2018/066.

Published
2021
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15. Additional file 1 of Catabolism of l-rhamnose in A. nidulans proceeds via the non-phosphorylated pathway and is glucose repressed by a CreA-independent mechanism

Author

MacCabe, Andrew P., Ninou, Elpinickie I., Pardo, Ester, and Orejas, Margarita

Abstract

Additional file 1: Figure S1. Schemes of microbial l-rhamnose catabolic pathways. (A) Catabolic pathways for l-rhamnose and (B) organisation of the genes encoding the corresponding activities in E. coli and various fungi. Homologous genes are indicated using the same colour code. Chr: chromosome number.

Published
2020
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18. Glucose uptake in germinating Aspergillus nidulans conidia: involvement of the creA and sorA genes

Author

MacCabe, Andrew P., Miro, Pilar, Ventura, Luisa, and Ramon, Daniel

Subjects
Aspergillus -- Genetic aspects, Gene mutations -- Physiological aspects, Carbohydrates -- Physiological aspects, Toxins -- Physiological aspects, Carbon -- Physiological aspects, Dextrose -- Physiological aspects, Glucose -- Physiological aspects, Microbiology -- Research, Biological sciences
Abstract

D-Glucose uptake in germinating wild-type Aspergillus nidulans conidia is an energy-requiring process mediated by at least two transport systems of differing affinities for glucose: a low-affinity system ([K.sub.m]~1.4 mM) and a high-affinity system ([K.sub.m]~16 [mu]M). The low-affinity system is inducible by glucose; the high-affinity system is subject to glucose repression effected by the carbon catabolite repressor CreA and is absent in sorA3 mutant conidia, which exhibit resistance to L-sorbose toxicity. An intermediate-affinity system ([K.sub.m]~400 [mu]M) is present in sorA3 conidia germinating in derepressing conditions, creA derepressed mutants show enhanced sensitivity to L-sorbose. The high-affinity uptake system appears to be responsible for the uptake of this toxic sugar.

Published
2003

20. The wide-domain carbon catabolite repressor CreA indirectly controls expression of the Aspergillus nidulans xlnB gene, encoding the acidic endo-beta-(1,4)-xylanase X(sub)24

Author

Orejas, Margarita, MacCabe, Andrew P., Perez-Gonzalez, Jose Antonio, Kumar, Sudeep, and Ramon, Daniel

Subjects
Aspergillus -- Genetic aspects, Microbial metabolism -- Genetic aspects, Gene expression -- Analysis, Sugar -- Microbiology, Biological sciences
Abstract

Results show that although mutation in the wide-domain repressor CreA gene leads to an elevated levels of xlnB mRNA, none of the CreA consensus target sites are localized in the xlnB gene. Data suggest that the catabolite repression of the xlnB encoded xylanase is exerted in an indirect manner.

Published
2001

23. Molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding a beta-xylosidase

Author

Perez-Gonzalez, Jose A., Peij, Noel N.M.E. van, Bezoen, Alja, MacCabe, Andrew P., Ramon, Daniel, and Graaff, Leo H. de

Subjects
Aspergillus -- Genetic aspects, Cloning -- Research, Genetic regulation -- Research, Biological sciences
Abstract

Research was conducted to examine the molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding the 85-kDA beta-xylosidase. The primary structure of the protein had considerable similarity to the primary structures of the Trichoderma reesei and Aspergillus niger beta-xylodases. Western blot analysis revealed elevated levels of production of the enzyme in overexpressing transformants showing that the cloned sequences existing in pXE1 encode the previously characterized 85-kDa beta-xylosidase.

Published
1998

24. Agrobacterium tumefaciens-Mediated Transformation of NHEJ Mutant Aspergillus nidulans Conidia: An Efficient Tool for Targeted Gene Recombination Using Selectable Nutritional Markers.

Author

Castillo, Virginia Casado-del, MacCabe, Andrew P., and Orejas, Margarita

Subjects
*AGROBACTERIUM tumefaciens, *ASPERGILLUS nidulans, *CONIDIA, *RECOMBINANT DNA, *TRANSGENE expression
Abstract

Protoplast transformation for the introduction of recombinant DNA into Aspergillus nidulans is technically demanding and dependant on the availability and batch variability of commercial enzyme preparations. Given the success of Agrobacterium tumefaciens-mediated transformation (ATMT) in diverse pathogenic fungi, we have adapted this method to facilitate transformation of A. nidulans. Using suitably engineered binary vectors, gene-targeted ATMT of A. nidulans non-homologous end-joining (NHEJ) mutant conidia has been carried out for the first time by complementation of a nutritional requirement (uridine/uracil auxotrophy). Site-specific integration in the DnkuA host genome occurred at high efficiency. Unlike other transformation techniques, however, cross-feeding of certain nutritional requirements from the bacterium to the fungus was found to occur, thus limiting the choice of auxotrophies available for ATMT. In complementation tests and also for comparative purposes, integration of recombinant cassettes at a specific locus could provide a means to reduce the influence of position effects (chromatin structure) on transgene expression. In this regard, targeted disruption of the wA locus permitted visual identification of transformants carrying site-specific integration events by conidial colour (white), even when auxotrophy selection was compromised due to cross-feeding. The protocol described offers an attractive alternative to the protoplast procedure for obtaining locus-targeted A. nidulans transformants. [ABSTRACT FROM AUTHOR]

Published
2021
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26. ORCHESTRA: Optimizable RF Converged Hardware Expression of a Scalable Transmit/Receive Architecture

Author

Benjamin McMahon, Maccabe Andrew C, Kunaal Boal, Daniel Fontaine, Jeffrey F. Bryant, Nicholas Campbell, Timothy M. Dresser, and Randall Lapierre

Subjects
business.industry, Computer science, Interface (computing), 020206 networking & telecommunications, 02 engineering and technology, 01 natural sciences, Expression (mathematics), 010305 fluids & plasmas, Open standard, 0103 physical sciences, Scalability, 0202 electrical engineering, electronic engineering, information engineering, Radio frequency, Architecture, business, Digital array, Digital signal processing, Computer hardware
Abstract

The need for disruptive advances in warfighter capability, upgradability, and utilization drives sensor systems toward convergent RF functionality with shared resources. The reliance of modern federated RF systems on redundant hardware leads to underutilization and inefficient use of SWaP-C. In pursuit of affordable solutions to this shortfall, converged RF and heterogeneous digital signal processing combine with existing open standard system-of-systems platform concepts to enable the next generation of platform and mission capability, through a true digital array interface to the RF environment.

Published
2018
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27. Identification of the genes encoding the catalytic steps corresponding to LRA4 (l‐2‐keto‐3‐deoxyrhamnonate aldolase) and l‐lactaldehyde dehydrogenase in Aspergillus nidulans: evidence for involvement of the loci AN9425/lraD and AN0544/aldA in the l‐rhamnose catabolic pathway

Author

MacCabe, Andrew, Sanmartín, Gemma, and Orejas, Margarita

Subjects
*ASPERGILLUS nidulans, *ALDEHYDE dehydrogenase, *GENES, *FILAMENTOUS fungi, *ALDOLASES, *ETHANOL, *ASPERGILLUS niger
Abstract

Summary: l‐rhamnose is found in nature mainly as a component of structural plant polysaccharides and can be used as a carbon source by certain microorganisms. Catabolism of this sugar in bacteria, archaea and fungi occurs by two routes involving either phosphorylated or non‐phosphorylated intermediates. Unlike the corresponding pathway in yeasts, the metabolic details of the non‐phosphorylated pathway in filamentous fungi are not fully defined. The first three genes (lraA, lraB and lraC) of the non‐phosphorylated pathway in Aspergillus nidulans have recently been studied revealing dependence on lraA function for growth on l‐rhamnose and α‐l‐rhamnosidase production. In the present work, two genes encoding the subsequent steps catalysed by l‐2‐keto‐3‐deoxyrhamnonate (l‐KDR) aldolase (AN9425) and l‐lactaldehyde dehydrogenase (AN0554) are identified. Loss‐of‐function mutations cause adverse growth effects on l‐rhamnose. Akin to genes lraA‐C and those encoding rhamnosidases (rhaA, rhaE), their expression is induced on l‐rhamnose via the transcriptional activator RhaR. Interestingly, the aldolase belongs to the ftablamily of bacterial l‐KDR aldolases (PF03328/COG3836) and not that of yeasts (PF00701/COG0329). In addition, AN0554 corresponds to the previously characterized aldA gene (encodes aldehyde dehydrogenase involved in ethanol utilization) thus revealing a previously unknown role for this gene in the catabolism of l‐rhamnose. [ABSTRACT FROM AUTHOR]

Published
2021
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28. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald P. de, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Akemi Uchima, Cristiane, Anderluh, Gregor, Asaollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Ozgur, Benocci, Tiziano, Braus-Stromeyer, Susanna A., Caldana, Camila, Cerqueira, Gustavo C., Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, Diallinas, George, Flipphi, Michel, Freyburg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, Maria Laura, Henrissat, Bernard, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karanyi, Zsolt, Krasevec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen L., Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio F., MacCabe, Andrew, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Ngan, Chew Yee, Orejas, Margarita, Ouedraogo, Jean Paul, Overkamp, Karin M., Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J., Ram, Arthur F.J., Ramon, Ana, Rauscher, Stefan, Record, Eric, Robert, Vincent, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S., Samson, Rob A., Sanguinetti, Manuel, Sep?i?, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio M., Sun, Hui, Susca, Antonia, Todd, Richard B., Tsang, Adrian, Unkles, Shiela E., Wiele, Nathalie van de, Rossen-Uffink, Diana van, Velasco de Castro Oliveira, Juliana, Vesth, Tammi C., Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miaomiao, Andersen, Mikael R., Archer, David B., Baker, Scott E., Benoit, Isabelle, Brakhage, Axel A., Braus, Gerhard H., Fischer, Reinhard, Frisvad, Jens C., Goldman, Gustavo H., Houbraken, Jos, Oakley, Berl, Scazzocchio, Claudio, Seiboth, Bernhard, vanKuyk, Patricia A., Wortman, Jennifer, Dyer, Paul S., and Grigoriev, Igor V.

Subjects
Genome sequencing, Comparative genomics, Fungal biology
Abstract

Background: The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus. Results: We have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli. Conclusions: Many aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.

Published
2017

29. Additional file 34: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Abstract

Histones of the Aspergilli. (PDF 526 kb)

Published
2017
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30. Additional file 23: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Abstract

Maximum likelihood tree of YAT transporters in the aspergilli. (PDF 89 kb)

Published
2017
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31. Additional file 8: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Subjects
body regions, inorganic chemicals, nervous system, fungi
Abstract

Effect of SHAM and KCN on fungal growth and sporulation. (PDF 252 kb)

Published
2017
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32. Additional file 7: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Abstract

Comparative growth profiling of the aspergilli and selected other fungi. (PDF 8.65 kb)

Published
2017
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33. Additional file 6: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Subjects
body regions, animal structures, nervous system, fungi
Abstract

Overview of number of putative orthologs/paralogs in the compared fungal species related to primary carbon metabolism. (PDF 237 kb)

Published
2017
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34. Additional file 5: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Subjects
body regions, nervous system, fungi, behavior and behavior mechanisms, reproductive and urinary physiology
Abstract

Expression of mating and pheromone-signalling pathway genes in representative asexual aspergilli. (PDF 488 kb)

Published
2017
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35. Additional file 15: of Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

Vries, Ronald De, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Sotiris Amillis, Cristiane Uchima, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Özgür, Benocci, Tiziano, Braus-Stromeyer, Susanna, Caldana, Camila, Cánovas, David, Cerqueira, Gustavo, Fusheng Chen, Wanping Chen, Choi, Cindy, Clum, Alicia, Santos, Renato Dos, Damásio, André, Diallinas, George, Emri, Tamás, Fekete, Erzsébet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, María, Henrissat, Bernard, Hildén, Kristiina, Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karányi, Zsolt, Kraševec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen, Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio, MacCabe, Andrew, Mäkelä, Miia, Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Márton, Molnár, Ákos, Mulé, Giuseppina, Chew Ngan, Orejas, Margarita, Orosz, Erzsébet, Ouedraogo, Jean, Overkamp, Karin, Park, Hee-Soo, Perrone, Giancarlo, Francois Piumi, Punt, Peter, Ram, Arthur, Ramón, Ana, Rauscher, Stefan, Record, Eric, Riaño-Pachón, Diego, Robert, Vincent, Röhrig, Julian, Ruller, Roberto, Salamov, Asaf, Nadhira Salih, Samson, Rob, Sándor, Erzsébet, Sanguinetti, Manuel, Schütze, Tabea, Sepčić, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio, Sun, Hui, Susca, Antonia, Todd, Richard, Tsang, Adrian, Unkles, Shiela, Wiele, Nathalie Van De, Rossen-Uffink, Diana Van, Oliveira, Juliana, Vesth, Tammi, Visser, Jaap, Jae-Hyuk Yu, Miaomiao Zhou, Andersen, Mikael, Archer, David, Baker, Scott, Benoit, Isabelle, Brakhage, Axel, Braus, Gerhard, Fischer, Reinhard, Frisvad, Jens, Goldman, Gustavo, Houbraken, Jos, Berl Oakley, Pócsi, István, Scazzocchio, Claudio, Seiboth, Bernhard, VanKuyk, Patricia, Wortman, Jennifer, Dyer, Paul, and Grigoriev, Igor

Abstract

Extended analysis of stress tolerance of the aspergilli. (PDF 706 kb)

Published
2017
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37. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

European Commission, Ministerio de Economía y Competitividad (España), Vries, Ronald P. de, Grigoriev, Igor V., MacCabe, Andrew P., Orejas, Margarita, European Commission, Ministerio de Economía y Competitividad (España), Vries, Ronald P. de, Grigoriev, Igor V., MacCabe, Andrew P., and Orejas, Margarita

Abstract

[Background] The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus., [Results] We have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli., [Conclusions] Many aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.

Published
2017

38. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

de Vries, Ronald P., Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Uchima, Cristiane Akemi, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie W, Battaglia, Evy, Bayram, Ozgur, Benocci, Tiziano, Braus-Stromeyer, Susanna A., Caldana, Camila, Canovas, David, Cerqueira, Gustavo C., Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, Correa dos Santos, Renato Augusto, de Lima Damasio, Andre Ricardo, Diallinas, George, Emri, Tamas, Fekete, Erzsebet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Laura Harispe, Maria, Henrissat, Bernard, Hilden, Kristiina S., Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karanyi, Zsolt, Krasevec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt M, Lagendijk, Ellen L., Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika A, Lipzen, Anna M, Logrieco, Antonio F., Maccabe, Andrew, Makela, Miia R., Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Marton, Molnar, Akos P., Mule, Giuseppina, Ngan, Chew Yee, Orejas, Margarita, Orosz, Erzsebet, Ouedraogo, Jean Paul, Overkamp, Karin M, Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J, Ram, Arthur F J, Ramon, Ana, Rauscher, Stefan, Record, Eric, Riano-Pachon, Diego Mauricio, Robert, Vincent, Roehrig, Julian, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S., Samson, Rob A., Sandor, Erzsebet, Sanguinetti, Manuel, Schutze, Tabea, Sepcic, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, FabioM., Sun, Hui, Susca, Antonia, Todd, Richard B, Tsang, Adrian, Unkles, Shiela E., van de Wiele, Nathalie, van Rossen-Uffink, Diana, de Castro Oliveira, Juliana Velasco, Vesth, Tammi C., Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miaomiao, Andersen, Mikael R, Archer, David B., Baker, Scott E, Benoit, Isabelle, Brakhage, Axel A, Braus, Gerhard H., Fischer, Reinhard, Frisvad, Jens C., Goldman, Gustavo H., Houbraken, Jos, Oakley, Berl R, Pocsi, Istvan, Scazzocchio, Claudio, Seiboth, Bernhard, vanKuyk, Patricia A, Wortman, Jennifer Russo, Dyer, Paul S, Grigoriev, Igor V, de Vries, Ronald P., Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Uchima, Cristiane Akemi, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie W, Battaglia, Evy, Bayram, Ozgur, Benocci, Tiziano, Braus-Stromeyer, Susanna A., Caldana, Camila, Canovas, David, Cerqueira, Gustavo C., Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, Correa dos Santos, Renato Augusto, de Lima Damasio, Andre Ricardo, Diallinas, George, Emri, Tamas, Fekete, Erzsebet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Laura Harispe, Maria, Henrissat, Bernard, Hilden, Kristiina S., Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karanyi, Zsolt, Krasevec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt M, Lagendijk, Ellen L., Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika A, Lipzen, Anna M, Logrieco, Antonio F., Maccabe, Andrew, Makela, Miia R., Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Marton, Molnar, Akos P., Mule, Giuseppina, Ngan, Chew Yee, Orejas, Margarita, Orosz, Erzsebet, Ouedraogo, Jean Paul, Overkamp, Karin M, Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J, Ram, Arthur F J, Ramon, Ana, Rauscher, Stefan, Record, Eric, Riano-Pachon, Diego Mauricio, Robert, Vincent, Roehrig, Julian, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S., Samson, Rob A., Sandor, Erzsebet, Sanguinetti, Manuel, Schutze, Tabea, Sepcic, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, FabioM., Sun, Hui, Susca, Antonia, Todd, Richard B, Tsang, Adrian, Unkles, Shiela E., van de Wiele, Nathalie, van Rossen-Uffink, Diana, de Castro Oliveira, Juliana Velasco, Vesth, Tammi C., Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miaomiao, Andersen, Mikael R, Archer, David B., Baker, Scott E, Benoit, Isabelle, Brakhage, Axel A, Braus, Gerhard H., Fischer, Reinhard, Frisvad, Jens C., Goldman, Gustavo H., Houbraken, Jos, Oakley, Berl R, Pocsi, Istvan, Scazzocchio, Claudio, Seiboth, Bernhard, vanKuyk, Patricia A, Wortman, Jennifer Russo, Dyer, Paul S, and Grigoriev, Igor V

Published
2017

39. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

Author

de Vries, Ronald, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Akemi Uchima, Cristiane, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Ozgur, Benocci, Tiziano, Braus-Stromeyer, Susanna A., Caldana, Camila, Canovas, David, Cerqueira, Gustavo C., Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, Correa dos Santos, Renato Augusto, de Lima Damasio, Andre Ricardo, Diallinas, George, Emri, Tamas, Fekete, Erzsebet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, Maria Laura, Henrissat, Bernard, Hilden, Kristiina S., Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karanyi, Zsolt, Krasevec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen L., Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio F., MacCabe, Andrew, Makela, Miia R., Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Marton, Molnar, Akos P., Mule, Giuseppina, Ngan, Chew Yee, Orejas, Margarita, Orosz, Erzsebet, Ouedraogo, Jean Paul, Overkamp, Karin M., Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J., Ram, Arthur F.J., Ramon, Ana, Rauscher, Stefan, Record, Eric, Riano-Pachon, Diego Mauricio, Robert, Vincent, Rohrig, Julian, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S., Samson, Rob A., Sandor, Erzsebet, Sanguinetti, Manuel, Schutze, Tabea, Sepcic, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio M., Sun, Hui, Susca, Antonia, Todd, Richard B., Tsang, Adrian, Unkles, Sheila E., van de Wiele, Nathalie, van Rossen-Uffink, Diana, Velasco de Castro Oliveria, Juliana, Vesth, Tammi C., Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miamiao, Andersen, Mikael R., Archer, David B., Baker, Scott E., Benoit, Isabelle, Brakhage, Axel A., Braus, Gerhard H., Fischer, Reinhard, Frisvad, Jens C., Goldman, Gustavo H., Houbraken, Jos, Oakley, Berl, Pocsi, Istvan, Scazzocchio, Claudio, Seiboth, Bernhard, vanKuyk, Patricia A., Wortman, Jennifer, Dyer, Paul S., Grigoriev, Igor V., de Vries, Ronald, Riley, Robert, Wiebenga, Ad, Aguilar-Osorio, Guillermo, Amillis, Sotiris, Akemi Uchima, Cristiane, Anderluh, Gregor, Asadollahi, Mojtaba, Askin, Marion, Barry, Kerrie, Battaglia, Evy, Bayram, Ozgur, Benocci, Tiziano, Braus-Stromeyer, Susanna A., Caldana, Camila, Canovas, David, Cerqueira, Gustavo C., Chen, Fusheng, Chen, Wanping, Choi, Cindy, Clum, Alicia, Correa dos Santos, Renato Augusto, de Lima Damasio, Andre Ricardo, Diallinas, George, Emri, Tamas, Fekete, Erzsebet, Flipphi, Michel, Freyberg, Susanne, Gallo, Antonia, Gournas, Christos, Habgood, Rob, Hainaut, Matthieu, Harispe, Maria Laura, Henrissat, Bernard, Hilden, Kristiina S., Hope, Ryan, Hossain, Abeer, Karabika, Eugenia, Karaffa, Levente, Karanyi, Zsolt, Krasevec, Nada, Kuo, Alan, Kusch, Harald, LaButti, Kurt, Lagendijk, Ellen L., Lapidus, Alla, Levasseur, Anthony, Lindquist, Erika, Lipzen, Anna, Logrieco, Antonio F., MacCabe, Andrew, Makela, Miia R., Malavazi, Iran, Melin, Petter, Meyer, Vera, Mielnichuk, Natalia, Miskei, Marton, Molnar, Akos P., Mule, Giuseppina, Ngan, Chew Yee, Orejas, Margarita, Orosz, Erzsebet, Ouedraogo, Jean Paul, Overkamp, Karin M., Park, Hee-Soo, Perrone, Giancarlo, Piumi, Francois, Punt, Peter J., Ram, Arthur F.J., Ramon, Ana, Rauscher, Stefan, Record, Eric, Riano-Pachon, Diego Mauricio, Robert, Vincent, Rohrig, Julian, Ruller, Roberto, Salamov, Asaf, Salih, Nadhira S., Samson, Rob A., Sandor, Erzsebet, Sanguinetti, Manuel, Schutze, Tabea, Sepcic, Kristina, Shelest, Ekaterina, Sherlock, Gavin, Sophianopoulou, Vicky, Squina, Fabio M., Sun, Hui, Susca, Antonia, Todd, Richard B., Tsang, Adrian, Unkles, Sheila E., van de Wiele, Nathalie, van Rossen-Uffink, Diana, Velasco de Castro Oliveria, Juliana, Vesth, Tammi C., Visser, Jaap, Yu, Jae-Hyuk, Zhou, Miamiao, Andersen, Mikael R., Archer, David B., Baker, Scott E., Benoit, Isabelle, Brakhage, Axel A., Braus, Gerhard H., Fischer, Reinhard, Frisvad, Jens C., Goldman, Gustavo H., Houbraken, Jos, Oakley, Berl, Pocsi, Istvan, Scazzocchio, Claudio, Seiboth, Bernhard, vanKuyk, Patricia A., Wortman, Jennifer, Dyer, Paul S., and Grigoriev, Igor V.

Abstract

Background The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus. Results We have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli. Conclusions Many aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.

Published
2017

40. The 2008 update of the Aspergillus nidulans genome annotation: A community effort

Author

Wortman, Jennifer Russo, Gilsenan, Jane Mabey, Joardar, Vinita, Deegan, Jennifer, Clutterbuck, John, Andersen, Mikael R., Archer, David, Bencina, Mojca, Braus, Gerhard, Coutinho, Pedro, von Doehren, Hans, Doonan, John, Driessen, Arnold J. M., Durek, Pawel, Espeso, Eduardo, Fekete, Erzsebet, Flipphi, Michel, Garcia Estrada, Carlos, Geysens, Steven, Goldman, Gustavo, de Groot, Piet W. J., Hansen, Kim, Harris, Steven D., Heinekamp, Thorsten, Helmstaedt, Kerstin, Henrissat, Bernard, Hofmann, Gerald, Homan, Tim, Horio, Tetsuya, Horiuchi, Hiroyuki, James, Steve, Jones, Meriel, Karaffa, Levente, Karanyi, Zsolt, Kato, Masashi, Keller, Nancy, Kelly, Diane E., Kiel, Jan A. K. W., Kim, Jung-Mi, van der Klei, Ida J., Klis, Frans M., Kovalchuk, Andriy, Krasevec, Nada, Kubicek, Christian P., Liu, Bo, MacCabe, Andrew, Meyer, Vera, Mirabito, Pete, Miskei, Marton, Mos, Magdalena, Mullins, Jonathan, Nelson, David R., Nielsen, Jens, Oakley, Berl R., Osmani, Stephen A., Pakula, Tiina, Paszewski, Andrzej, Paulsen, Ian, Pilsyk, Sebastian, Pocsi, Istvan, Punt, Peter J., Ram, Arthur F. J., Ren, Qinghu, Robellet, Xavier, Robson, Geoff, Seiboth, Bernhard, van Solingen, Piet, Specht, Thomas, Sun, Jibin, Taheri-Talesh, Naimeh, Takeshita, Norio, Ussery, Dave, Vankuyk, Patricia A., Visser, Hans, de Vondervoort, Peter J. I. van, Walton, Jonathan, Xiang, Xin, Xiong, Yi, Zeng, An Ping, Brandt, Bernd W., Cornell, Michael J., van den Hondel, Cees A. M. J. J., Visser, Jacob, Oliver, Stephen G., Turner, Geoffrey, Kraševec, Nada, Kuyk, Patricia A. van, Döhren, D.H., van Seilboth, B, de Vries, R., Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Instituto de Agroquimica y Tecnologia de Alimentos (IATA), Instituto de Agroquímica y Tecnología de Alimentos - Institute of Agrochemistry and Food Technology [Valencia] (IATA-CSIC), Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology, Molecular Cell Biology, Faculty of Science and Engineering, Host-Microbe Interactions, Man, Biomaterials and Microbes (MBM), Centre for World Food Studies, Bioinformatics, Bio Informatics (IBIVU), Integrative Bioinformatics, Molecular Biology and Microbial Food Safety (SILS, FNWI), and Mass Spectrometry of Biomacromolecules (SILS, FNWI)

Subjects
GENE-CLUSTER, PREDICTION, Assembly, Biológiai tudományok, CADRE, Genome, Természettudományok, CELL-WALL, Genetics, 0303 health sciences, Fungal protein, biology, MESH: Aspergillus nidulans, MESH: Genomics, Fungal community, Genomics, Genome project, Fungal, Aspergillus, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Genome, Fungal, Identification (biology), MESH: Fungal Proteins, Genome, Fungal, fungal community, Annotation, Genes, Fungal, Computational biology, Vertebrate and Genome Annotation Project, SEQUENCE, Microbiology, Article, Aspergillus nidulans, Fungal Proteins, 03 medical and health sciences, transcription factors, Transcription factors, BIOSYNTHESIS, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, A-FUMIGATUS, genome, 030304 developmental biology, IDENTIFICATION, 030306 microbiology, DNA, NIGER, biology.organism_classification, TRANSCRIPTIONAL ACTIVATOR, Genes, Aspergilli, MESH: Genes, Fungal
Abstract

12 páginas, 2 tablas -- PAGS nros. S2-S13, et al, The identification and annotation of protein-coding genes is one of the primary goals of whole-genome sequencing projects, and the accuracy of predicting the primary protein products of gene expression is vital to the interpretation of the available data and the design of downstream functional applications. Nevertheless, the comprehensive annotation of eukaryotic genomes remains a considerable challenge. Many genomes submitted to public databases, including those of major model organisms, contain significant numbers of wrong and incomplete gene predictions. We present a community-based reannotation of the Aspergillus nidulans genome with the primary goal of increasing the number and quality of protein functional assignments through the careful review of experts in the field of fungal biology, We acknowledge financial support by the European Commission under contract LSSG-CT-2005-018964

Published
2009
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42. Endoxilanasa termorresistente obtenida por mutagénesis y su aplicación al proceso de obtención de bioetanol

Author

MacCabe, Andrew P., Polaina Molina, Julio, Sánchez Hervás, Meritxell, Vallés Alventosa, Salvador, Manzanares, Paloma, Martorell Guerola, Patricia, Ramón, Daniel, and Genovés, Salvador

Subjects
food and beverages, humanities
Abstract

[EN] The present invention describes a peptide sequence in which a series of amino acids has been substituted by site-directed mutagenesis from the sequence encoding Aspergillus nidulans endoxylanase, in order to obtain an endoxylanase that has greater thermal stability. Furthermore, the present invention relates to the use of this endoxylanase for obtaining the xylan from plant material and to the subsequent use of this xylan in bioethanol production processes ., [ES] La presente invención describe una secuencia peptídica a la se han sustituido una serie de aminoácidos por mutagénesis dirigida a partir de la secuencia codificante de endoxilanasa de Aspergillus nidulans para obtener una endoxilanasa que presenta una mayor estabilidad térmica. Además, la presente invención se refiere al uso de esta endoxilanasa para la obtención de xilano a partir de materia vegetal y al posterior uso de este xilano en procesos de obtención de bioetanol., Consejo Superior de Investigaciones Científicas (España), Biopolis SL, A1 Solicitud de patente con informe sobre el estado de la técnica

Published
2010

43. Heat-resistant endoxylanase obtained by mutagenesis and the use thereof in the bioethanol production process

Author

MacCabe, Andrew P., Polaina Molina, Julio, Sánchez Hervás, Meritxell, Vallés Alventosa, Salvador, Manzanares, Paloma, Martorell Guerola, Patricia, Ramón, Daniel, and Genovés, Salvador

Abstract

[EN] The present invention describes a peptide sequence in which a series of amino acids has been substituted by site-directed mutagenesis from the sequence encoding Aspergillus nidulans endoxylanase, in order to obtain an endoxylanase that has greater thermal stability. Furthermore, the present invention relates to the use of this endoxylanase for obtaining the xylan from plant material and to the subsequent use of this xylan in bioethanol production processes . [ES] La presente invención describe una secuencia peptídica a la se han sustituido una serie de aminoácidos por mutagénesis dirigida a partir de la secuencia codificante de endoxilanasa de Aspergillus nidulans para obtener una endoxilanasa que presenta una mayor estabilidad térmica. Además, la presente invención se refiere al uso de esta endoxilanasa para la obtención de xilano a partir de materia vegetal y al posterior uso de este xilano en procesos de obtención de bioetanol. Peer reviewed Consejo Superior de Investigaciones Científicas (España), Biopolis SL A1 Solicitud de patente con informe sobre el estado de la técnica

Published
2010

44. High-affinity glucose transport in Aspergillus nidulans is mediated by the products of two related but differentially expressed genes

Author

Ministerio de Educación y Ciencia (España), European Commission, Ministerio de Economía y Competitividad (España), Forment, Josep V., Flipphi, Michel, Ventura, Luisa, González García, Ramón, Ramón, Daniel, MacCabe, Andrew P., Ministerio de Educación y Ciencia (España), European Commission, Ministerio de Economía y Competitividad (España), Forment, Josep V., Flipphi, Michel, Ventura, Luisa, González García, Ramón, Ramón, Daniel, and MacCabe, Andrew P.

Abstract

Independent systems of high and low affinity effect glucose uptake in the filamentous fungus Aspergillus nidulans. Low-affinity uptake is known to be mediated by the product of the mstE gene. In the current work two genes, mstA and mstC, have been identified that encode high-affinity glucose transporter proteins. These proteins' primary structures share over 90% similarity, indicating that the corresponding genes share a common origin. Whilst the function of the paralogous proteins is little changed, they differ notably in their patterns of expression. The mstC gene is expressed during the early phases of germination and is subject to CreA-mediated carbon catabolite repression whereas mstA is expressed as a culture tends toward carbon starvation. In addition, various pieces of genetic evidence strongly support allelism of mstC and the previously described locus sorA. Overall, our data define MstC/SorA as a high-affinity glucose transporter expressed in germinating conidia, and MstA as a high-affinity glucose transporter that operates in vegetative hyphae under conditions of carbon limitation. © 2014 Forment et al.

Published
2014

46. Heat-resistant endoxylanase obtained by mutagenesis and the use thereof in the bioethanol production process

Author

MacCabe, Andrew P., Polaina Molina, Julio, Sánchez Hervás, Meritxell, Vallés Alventosa, Salvador, Manzanares, Paloma, Martorell Guerola, Patricia, Ramón, Daniel, Genovés, Salvador, MacCabe, Andrew P., Polaina Molina, Julio, Sánchez Hervás, Meritxell, Vallés Alventosa, Salvador, Manzanares, Paloma, Martorell Guerola, Patricia, Ramón, Daniel, and Genovés, Salvador

Abstract

[EN] The present invention describes a peptide sequence in which a series of amino acids has been substituted by site-directed mutagenesis from the sequence encoding Aspergillus nidulans endoxylanase, in order to obtain an endoxylanase that has greater thermal stability. Furthermore, the present invention relates to the use of this endoxylanase for obtaining the xylan from plant material and to the subsequent use of this xylan in bioethanol production processes ., [ES] La presente invención describe una secuencia peptídica a la se han sustituido una serie de aminoácidos por mutagénesis dirigida a partir de la secuencia codificante de endoxilanasa de Aspergillus nidulans para obtener una endoxilanasa que presenta una mayor estabilidad térmica. Además, la presente invención se refiere al uso de esta endoxilanasa para la obtención de xilano a partir de materia vegetal y al posterior uso de este xilano en procesos de obtención de bioetanol.

Published
2011

47. Endoxilanasa termorresistente obtenida por mutagénesis y su aplicación al proceso de obtención de bioetanol

Author

MacCabe, Andrew P., Polaina Molina, Julio, Sánchez Hervás, Meritxell, Vallés Alventosa, Salvador, Manzanares, Paloma, Martorell Guerola, Patricia, Ramón, Daniel, Genovés, Salvador, MacCabe, Andrew P., Polaina Molina, Julio, Sánchez Hervás, Meritxell, Vallés Alventosa, Salvador, Manzanares, Paloma, Martorell Guerola, Patricia, Ramón, Daniel, and Genovés, Salvador

Abstract

Endoxilanasa termorresistente obtenida por mutagénesis y su aplicación al proceso de obtención de bioetanal. La presente invención describe una secuencia peptídica a la se han sustituido una serie de aminoácidos por mutagénesis dirigida a partir de la secuencia codificante de ndoxilanasa de Aspergillus nidulans para obtener una endoxilanasa que presenta una mayor estabilidad térmica. Además, la presente invención se refiere al uso de esta endoxilanasa para la obtención de xilano a partir de materia vegetal y al posterior uso de este xilano en procesos de obtención de bioetanol.

Published
2011

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