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Bariatric surgery (BS) is a dependable method for managing obesity and metabolic diseases, however, the regulatory processes of lipid metabolism are still not well elucidated. Differentially expressed genes (DEGs) were analysed through three transcriptomic datasets of GSE29409, GSE59034 and GSE72158 from the GEO database regarding subcutaneous adipose tissue (SAT) after BS, and 37 DEGs were identified. The weighted gene co-expression network analysis (WGCNA), last absolute shrinkage and selection operator (LASSO) logistic regression and support vector machine-recursive feature elimination (SVM-RFE) algorithms further screened four key genes involved in the regulation of STMN2, SFRP4, APOE and MXRA5. The GSE53376 dataset was used to further confirm the differential expression of SFRP4, APOE and MXRA5 in the postoperative period. GSEA analysis reveals activation of immune-related regulatory pathways after surgery. Finally, the silencing of MXRA5 was found by experimental methods to affect the expression of PPARγ and CEBPα during the differentiation of preadipocytes, as well as to affect the formation of lipid droplets. In conclusion, SAT immunoregulation was mobilized after BS, while MXRA5 was involved in the regulation of lipid metabolism.

IDH-wildtype glioblastoma (GBM) is the most common and malignant primary brain tumor. The purpose of this study is to establish a prognostic gene signature for IDH-wildtype GBM. RNA sequencing data of normal brain tissue and GBM patients were obtained from TCGA, CGGA, GEO and the GTEx databases. Identification of prognostic differentially expressed genes (DEGs) with | log2 fold change | > 0.5 and adjust p < 0.05 in TCGA and CGGA databases by "limma" method. By LASSO regression analysis and multivariate Cox analysis, a 3-gene prognostic signature composed of FMOD, MXRA5 and RAB36 was established. The 3-gene prognostic risk model is validated by TCGA and GSE43378 datasets. The expression of FMOD, MXRA5 and RAB36 in GBM patients was significantly higher than that in normal brain tissues in CCGA, TCGA and GSE29796 data sets. In order to further verify this result, total RNA was extracted from tumors and paracancerous tissues of 9 GBM patients. RT-PCR results showed that the expression of FMOD, MXRA5 and RAB36 in tumor tissues of most patients was higher than that in paracancerous tissues. The results of GSEA showed that the pathway enrichment of the 3-gene signature was mainly related to tumor immunity. Immune cell infiltration analyzed by ssGSEA showed that there were significant differences in macrophages between high- and low-risk groups. Immune checkpoint genes correlation analysis showed that PD-L1 gene expression is closely related to risk score. Our study identifies a prognostic-associated risk model and provides a potential effective immunotherapy target for IDH-wildtype GBM patients., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)

Recent investigations have described the use of urinary matrix remodeling-associated protein 5 (MXRA5) as a novel biomarker of kidney impairment in the setting of chronic kidney disease. In this study, we aimed to evaluate the possible clinical application of urinary MXRA5 as a useful non-invasive marker in the urine from the affected renal pelvis and bladder of children with ureteropelvic junction obstruction (UPJO). We conducted a prospective cohort study of patients aged

Cancer-associated fibroblasts (CAFs) are prevalent in the tumor microenvironment of breast cancer, comprising a group of cell subpopulations with spatial, phenotypic, and functional heterogeneity. Due to the lack of specific markers for CAF subpopulations, their specific mechanisms in breast cancer remain unclear. We identified eight distinct CAF phenotypes in breast cancer using multiple single-cell RNA sequencing datasets and determined distinct transcription factors (TFs) of CAFs through SCENIC analysis. Our study highlights one CAF subtype in breast cancer, FN1+CAF2, associated with metastasis and macrophage polarization. We observed elevated FN1 expression in the stromal tissue of breast cancer patients. Furthermore, FN1 knockdown in CAFs reduced the migration ability of breast cancer cells. We identified a regulatory gene, MXRA5, in CAF2, which may play crucial roles in breast cancer. Our results indicated upregulated MXRA5 expression in breast cancer tissues and CAFs from patients with lymph node metastasis in the following experiment. Overall, our study reveals that the FN1+CAF2 subtype is associated with metastasis and suggests that MXRA5 may be a novel marker mediating the effects of CAF2 on breast cancer metastasis. This study enriches our understanding of CAF heterogeneity and offers new insights for treating breast cancer metastasis., Competing Interests: None., (AJCR Copyright © 2024.)

Matrix remodeling associated 5 (MXRA5) is an extracellular protein that is upregulated in several cancers, but little is known regarding its spatial and temporal localization in the developing embryo. The present study was undertaken to investigate MXRA5 transcript expression in the trunk and limb of the embryonic chick to provide groundwork for future investigation of its developmental function. In situ hybridization utilizing digoxigenin-labeled sense control and experimental antisense probes was performed in paraffin sections of chick embryos from Hamburger and Hamilton (HH) stages 18-38. MXRA5 expression was initially low and restricted in extent at the earliest stages examined, but expression increased in strength and tissue distribution with developmental age. Transcripts were largely found in cells of mesodermal origin, including gut associated mesenchyme, tendon and ligament rudiments, intervertebral discs, dermal papillae of feather buds, heart valve precursors and leaflets, as well as in appendicular joint primordia. The present study has provided initial information on MXRA5 gene expression in the trunk and limb of early to mid-stage avian embryos. Results show that MXRA5 was expressed most strongly at sites undergoing change and remodeling of the extracellular matrix during transition of embryonic tissues into the functional adult morphology. [ABSTRACT FROM AUTHOR]

Preeclampsia (PE) may induce gestational failure threatening a significant number of pregnant women. Dysfunctional placental trophoblast cells have an important impact on PE progression. microRNAs (miRNAs) have been reported to participate in PE progression, whereas the mechanism that underlies miR-30b involved in PE progression and function of placental trophoblast cells remains poorly understood. Cell viability was investigated by cell counting kit-8 (CCK-8) assay. Cell apoptosis was detected by flow cytometry using Annexin V-FITC/propidium iodide (PI) staining. Cell invasion was analyzed by trans-well assay. The expression of miR-30b was measured by quantitative real-time polymerase chain reaction (qRT-PCR). The abundance of matrix-remodeling associated 5 (MXRA5) protein was detected by western blots (WB). The interaction between miR-30b and MXRA5 was investigated by bioinformatics analysis and luciferase activity assay. The effect of miR-30b and MXRA5 on mitogen-activated protein kinases (MAPK) pathway and invasion was evaluated by WB. Then we found miR-30b was highly expressed in PE and its overexpression inhibited cell viability and invasion while enhanced apoptosis in JEG-3 and HTR8/SVneo cells. Moreover, MXRA5 was targeted by miR-30b and MXRA5 restoration attenuated the effect of miR-30b on cell processes in HTR8/SVneo cells. Besides, both of miR-30b and MXRA5 were associated with MAPK pathway in HTR8/SVneo cells. Our data suggested miR-30b might contribute to PE through inhibiting cell viability, invasion while inducing apoptosis of placental trophoblast cells via MAPK pathway by targeting MXRA5. These indicated that miR-30b might be a novel biomarker for PE treatment., Competing Interests: None., (IJCEP Copyright © 2019.)

In our previous study, significantly high expression levels of matrix-remodeling associated 5 (MXRA5) were identified in fresh-cultured colorectal cancer (CRC) tissues compared with their normal adjacent mucosa by differential secretome analysis. Whether MXRA5 is a potential serum biomarker of CRC has not been evaluated. The aim of this study was to investigate the association between MXRA5 expression and clinicopathological characteristics of CRC patients. The MXRA5 expression levels were determined by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC) in 20 colorectal adenoma tissues, 156 CRC tissues and their corresponding adjacent normal mucosa. Relative quantity (RQ) value and immunoreactive score (IRS) were used for quantitative assessment. The staining for MXRA5 protein was mainly located in the cytoplasm of CRC cells. All CRC tissues were positively stained, with a higher expression rate (IRS>4) of 67% (105/156), and a lower expression rate (IRS≤4) of 33% (51/156). Meanwhile, their corresponding normal tissues exhibited little positive staining; the higher expression rate was 0% (0/156) and the lower expression rate was 25% (16/156). Additionally, more than half of the adenoma tissues were positively stained; the higher expression rate was 15% (3/20) and the lower expression rate was 50% (10/20). The MXRA5 protein positive staining rates were significantly correlated with the lesion sites (colon vs. rectum, 76 vs. 59%), TNM staging (I+II vs. III+IV, 56 vs. 73%) and metastasis (present vs. absent; 76 vs. 61%) with the most high positive staining rate observable in omental metastasis (82%). However, MXRA5 mRNA expression levels showed no significant differences between CRC tissues and their corresponding normal tissues, and no significant correlation between IRS and corresponding RQ value was observed. In this study, we present the first evaluation of MXRA5 protein expression in CRC tissue. Our results revealed that MXRA5 protein is aberrantly expressed in CRC tissues, and has potential value in early detection of CRC and prediction of omental metastasis.