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Identification and functional activity of matrix-remodeling associated 5 (MXRA5) in benign hyperplastic prostate
- Source :
- Aging (Albany NY)
- Publication Year :
- 2020
- Publisher :
- Impact Journals, LLC, 2020.
-
Abstract
- Objective Benign prostatic hyperplasia (BPH) is a common condition in aging males. The current study aims to identify differentially expressed genes (DEGs) associated with BPH and to elucidate the role of matrix-remodeling associated 5 (MXRA5) protein and mitogen-activated protein kinase (MAPK) signaling pathways in BPH. Results A total of 198 DEGs and a number of related pathways were identified with MXRA5 being one of the most significantly altered DEGs. MXRA5 was upregulated in BPH samples and localized mostly in stroma. Knockdown of MXRA5 induced stromal cell cycle arrest instead of inhibiting apoptosis. Consistently, MXRA5 overexpression enhanced epithelial cell proliferation. In addition, phosphorylated ERK1/2 and p38, key members of the MAPK family, were strongly decreased with knockdown but increased with overexpression. Conclusion Our novel data demonstrates that upregulation of MXRA5 in the enlarged prostate could contribute to the development of BPH through increasing cell proliferation via the MAPK pathway. Thus, the MXRA5-MAPK system could be rediscovered as a new therapeutic target for treating BPH. Methods Microarray analysis and integrated bioinformatics were conducted. The expression and biologic functions of MXRA5 was investigated via RT-PCR, western-blot, immunofluorescence, flow cytometry and MTT assay. Finally, genes involved in regulation of the MAPK pathway were investigated.
- Subjects :
- Male
MAPK/ERK pathway
Aging
Stromal cell
MAP Kinase Signaling System
Prostatic Hyperplasia
Biology
Cell Line
expression profiling by array
Downregulation and upregulation
Humans
Protein kinase A
Cell Proliferation
benign prostatic hyperplasia
Gene knockdown
Microarray analysis techniques
Cell growth
MXRA5
Cell Biology
Up-Regulation
MAPK signaling pathway
Cancer research
Proteoglycans
Mitogen-Activated Protein Kinases
Signal transduction
Research Paper
Subjects
Details
- ISSN :
- 19454589
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Aging
- Accession number :
- edsair.doi.dedup.....32d59a9e571b03511eecb4084a6d61b8
- Full Text :
- https://doi.org/10.18632/aging.103175