1. Diversity surveys and evolutionary relationships of aoxB genes in aerobic arsenite-oxidizing bacteria
- Author
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Marianne Quéméneur, Michel Jauzein, Audrey Heinrich-Salmeron, Didier Lièvremont, Catherine Joulian, Daniel Muller, Philippe N. Bertin, Francis Garrido, Génétique moléculaire, génomique, microbiologie (GMGM), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Laboratoire de Biologie Tumorale, CRLCC Paul Strauss, XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Bureau de Recherches Géologiques et Minières (BRGM) (BRGM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Génétique moléculaire, génomique, microbiologie ( GMGM ), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique ( CNRS ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), XLIM ( XLIM ), Université de Limoges ( UNILIM ) -Centre National de la Recherche Scientifique ( CNRS ), and Bureau de Recherches Géologiques et Minières (BRGM) ( BRGM )
- Subjects
Chemoautotrophic Growth ,MESH: Sequence Analysis, Protein ,MESH: Amino Acid Sequence ,MESH: Base Sequence ,Polymerase Chain Reaction ,MESH : Arsenites ,Conserved sequence ,Sequence Analysis, Protein ,RNA, Ribosomal, 16S ,MESH : Genetic Markers ,MESH : DNA, Bacterial ,Soil Pollutants ,MESH: Genetic Variation ,MESH: Phylogeny ,MESH : Proteobacteria ,Phylogeny ,0303 health sciences ,MESH: Conserved Sequence ,MESH : Amino Acid Sequence ,MESH : Genes, Bacterial ,Bacterial ,MESH : Oxidoreductases ,MESH: RNA, Ribosomal, 16S ,MESH : DNA Primers ,MESH: Arsenites ,Proteobacteria ,Genetic Markers ,MESH: DNA Primers ,16S ,Sequence analysis ,Arsenites ,MESH : Soil Microbiology ,Molecular Sequence Data ,MESH: Biodiversity ,03 medical and health sciences ,Phylogenetics ,MESH : Conserved Sequence ,MESH : Bacteria ,Amino Acid Sequence ,MESH: Oxidoreductases ,[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,MESH : Oxidation-Reduction ,Ribosomal ,MESH: Soil Pollutants ,MESH: Molecular Sequence Data ,Bacteria ,030306 microbiology ,Alphaproteobacteria ,Genetic Variation ,MESH: Polymerase Chain Reaction ,DNA ,Ribosomal RNA ,MESH: DNA, Bacterial ,Genes ,MESH : Sequence Analysis, Protein ,MESH : Sequence Analysis, DNA ,MESH: Oxidation-Reduction ,MESH: Sequence Analysis, DNA ,MESH : Molecular Sequence Data ,MESH: Genetic Markers ,Applied Microbiology and Biotechnology ,MESH : Soil Pollutants ,MESH : Biodiversity ,MESH : Polymerase Chain Reaction ,Betaproteobacteria ,Conserved Sequence ,Soil Microbiology ,Genetics ,Ecology ,biology ,Biodiversity ,MESH: Genes, Bacterial ,Oxidoreductases ,Oxidation-Reduction ,Sequence Analysis ,Biotechnology ,DNA, Bacterial ,MESH : Chemoautotrophic Growth ,Microbial Ecology ,MESH : Genetic Variation ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,030304 developmental biology ,DNA Primers ,Base Sequence ,Protein ,MESH : Phylogeny ,Sequence Analysis, DNA ,MESH: Proteobacteria ,biology.organism_classification ,16S ribosomal RNA ,MESH: Chemoautotrophic Growth ,MESH : RNA, Ribosomal, 16S ,MESH: Bacteria ,MESH: Soil Microbiology ,Genes, Bacterial ,RNA ,MESH : Base Sequence ,Food Science - Abstract
A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria -, Betaproteobacteria -, and Gammaproteobacteria -related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.
- Published
- 2008