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Diversity surveys and evolutionary relationships of aoxB genes in aerobic arsenite-oxidizing bacteria
- Source :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, American Society for Microbiology, 2008, 74 (14), pp.4567-73. ⟨10.1128/AEM.02851-07⟩, Applied and Environmental Microbiology, American Society for Microbiology, 2008, 74 (14), pp.4567-73. 〈10.1128/AEM.02851-07〉
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria -, Betaproteobacteria -, and Gammaproteobacteria -related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.
- Subjects :
- Chemoautotrophic Growth
MESH: Sequence Analysis, Protein
MESH: Amino Acid Sequence
MESH: Base Sequence
Polymerase Chain Reaction
MESH : Arsenites
Conserved sequence
Sequence Analysis, Protein
RNA, Ribosomal, 16S
MESH : Genetic Markers
MESH : DNA, Bacterial
Soil Pollutants
MESH: Genetic Variation
MESH: Phylogeny
MESH : Proteobacteria
Phylogeny
0303 health sciences
MESH: Conserved Sequence
MESH : Amino Acid Sequence
MESH : Genes, Bacterial
Bacterial
MESH : Oxidoreductases
MESH: RNA, Ribosomal, 16S
MESH : DNA Primers
MESH: Arsenites
Proteobacteria
Genetic Markers
MESH: DNA Primers
16S
Sequence analysis
Arsenites
MESH : Soil Microbiology
Molecular Sequence Data
MESH: Biodiversity
03 medical and health sciences
Phylogenetics
MESH : Conserved Sequence
MESH : Bacteria
Amino Acid Sequence
MESH: Oxidoreductases
[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology
MESH : Oxidation-Reduction
Ribosomal
MESH: Soil Pollutants
MESH: Molecular Sequence Data
Bacteria
030306 microbiology
Alphaproteobacteria
Genetic Variation
MESH: Polymerase Chain Reaction
DNA
Ribosomal RNA
MESH: DNA, Bacterial
Genes
MESH : Sequence Analysis, Protein
MESH : Sequence Analysis, DNA
MESH: Oxidation-Reduction
MESH: Sequence Analysis, DNA
MESH : Molecular Sequence Data
MESH: Genetic Markers
Applied Microbiology and Biotechnology
MESH : Soil Pollutants
MESH : Biodiversity
MESH : Polymerase Chain Reaction
Betaproteobacteria
Conserved Sequence
Soil Microbiology
Genetics
Ecology
biology
Biodiversity
MESH: Genes, Bacterial
Oxidoreductases
Oxidation-Reduction
Sequence Analysis
Biotechnology
DNA, Bacterial
MESH : Chemoautotrophic Growth
Microbial Ecology
MESH : Genetic Variation
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
030304 developmental biology
DNA Primers
Base Sequence
Protein
MESH : Phylogeny
Sequence Analysis, DNA
MESH: Proteobacteria
biology.organism_classification
16S ribosomal RNA
MESH: Chemoautotrophic Growth
MESH : RNA, Ribosomal, 16S
MESH: Bacteria
MESH: Soil Microbiology
Genes, Bacterial
RNA
MESH : Base Sequence
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 00992240 and 10985336
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, American Society for Microbiology, 2008, 74 (14), pp.4567-73. ⟨10.1128/AEM.02851-07⟩, Applied and Environmental Microbiology, American Society for Microbiology, 2008, 74 (14), pp.4567-73. 〈10.1128/AEM.02851-07〉
- Accession number :
- edsair.doi.dedup.....9087b98bc3478b2904fa9f14da1a716f