1. Electrostatic wipes as simple and reliable methods for influenza virus airborne detection
- Author
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Régis Aubry, A. Debernardi, Catherine Chirouze, N. Magy-Bertrand, Kevin Bouiller, J.L. Prétet, L. Puget, Quentin Lepiller, Solène Marty-Quinternet, Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon), Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (EA 3181) (CEF2P / CARCINO), Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Chrono-environnement - CNRS - UFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), and Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE)
- Subjects
Microbiology (medical) ,Static Electricity ,Air Microbiology ,Congenital cytomegalovirus infection ,MESH: Limit of Detection ,Influenza virus A ,030501 epidemiology ,Airborne detection ,Virus ,03 medical and health sciences ,MESH: Air Microbiology ,Limit of Detection ,Influenza, Human ,Patients' Rooms ,medicine ,Humans ,Electrostatic wipes ,MESH: Static Electricity ,MESH: Orthomyxoviridae ,Detection limit ,0303 health sciences ,MESH: Humans ,SIMPLE (military communications protocol) ,030306 microbiology ,business.industry ,MESH: Influenza, Human ,MESH: Patients' Rooms ,Reproducibility of Results ,virus diseases ,RNA ,General Medicine ,Orthomyxoviridae ,medicine.disease ,Virology ,3. Good health ,Virus detection ,MESH: Reproducibility of Results ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Infectious Diseases ,0305 other medical science ,business ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
International audience; The performance of an in-house protocol for virus detection on commercialized electrostatic wipes (EWs) was assessed experimentally by impregnating them with suspensions of cytomegalovirus, adenovirus, and influenza virus, and by determining the recovery efficiency, repeatability, and detection limit of the protocol. The protocol was sensitive enough to detect 4 log10 gene copies of virus. At room temperature, influenza RNA was stable on EWs for at least four days. When EWs were placed high in 32 influenza-infected patients' rooms, influenza RNA was detectable in 75% (N = 24) of EWs, suggesting that EWs are simple and reliable methods for influenza virus airborne detection.
- Published
- 2021
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