1. Methanohalophilus profundi sp. nov., a methylotrophic halophilic piezophilic methanogen isolated from a deep hypersaline anoxic basin
- Author
-
Laurent Toffin, Stéphane L'Haridon, Charlotte Balière, Morgane Chalopin, Erwan Corre, Mohamed Jebbar, Violetta La Cono, Hani Haroun, Michail M. Yakimov, Patricia Pignet, Erwan Roussel, Laboratoire de microbiologie des environnements extrêmophiles (LM2E), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (ABIMS), Fédération de recherche de Roscoff (FR2424), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Insitute for Coastal Marine Environment, National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), This study was funded by the European Union program MaCuMBA (grant agreement 311975) and the MAMBA project (FP7-KBBE-2008–226977). Research was supported by UBO, CNRS and Ifremer., European Project: 311975,EC:FP7:KBBE,FP7-KBBE-2012-6-singlestage,MACUMBA(2012), European Project: 226977,EC:FP7:KBBE,FP7-KBBE-2008-2B,MAMBA(2009), Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (FR2424), and Consiglio Nazionale delle Ricerche (CNR)
- Subjects
Salinity ,MESH: Hydrogen-Ion Concentration ,Hydrostatic pressure ,MESH: Methanol ,Applied Microbiology and Biotechnology ,Genes, Archaeal ,MESH: Methylamines ,RNA, Ribosomal, 16S ,Anaerobiosis ,MESH: Phylogeny ,Phylogeny ,MESH: Methanosarcinaceae ,Methanosarcinaceae ,0303 health sciences ,biology ,Strain (chemistry) ,Temperature ,Hydrogen-Ion Concentration ,Methanogen ,MESH: Temperature ,Halophile ,MESH: RNA, Ribosomal, 16S ,RNA, Bacterial ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Methanosarcinales ,Water Microbiology ,MESH: RNA, Bacterial ,Deep hypersalin anoxic basin (DHAB) ,MESH: Mediterranean Sea ,Methanohalophilus ,Microbiology ,MESH: Genes, Archaeal ,Methylamines ,03 medical and health sciences ,MESH: Hydrostatic Pressure ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,MESH: Anaerobiosis ,Botany ,Hydrostatic Pressure ,Mediterranean Sea ,Piezophilic ,Seawater ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,030306 microbiology ,Methanol ,MESH: Seawater ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,16S ribosomal RNA ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,MESH: Water Microbiology ,Methylotrophic ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,MESH: Salinity - Abstract
International audience; A novel anaerobic methylotrophic halophilic methanogen strain SLHTYRO T was isolated from a deep hypersaline anoxic basin called "Tyro" located in the Eastern Mediterranean Sea. Cells of SLHTYRO T were motile cocci. The strain SLHTYRO T grew between 12 and 37 °C (optimum 30 °C), at pH between 6.5 and 8.2 (optimum pH 7.5) and salinity from 45 to 240 g L −1 NaCl (optimum 135 g L −1). Strain SLHTYRO T was methylotrophic methanogen able to use methylated compounds (trimethylamine, dimethylamine, monomethylamine and methanol). Strain SLHTYRO T was able to grow at in situ hydrostatic pressure and temperature conditions (35 MPa, 14 °C). Phylogenetic analysis based on 16S rRNA gene and mcrA gene sequences indicated that strain SLHTYRO T was affiliated to genus Methanohalophilus within the order Methanosarcinales. It shared >99.16% of the 16S rRNA gene sequence similarity with strains of other Methanohalophilus species. Based on ANIb, AAI and dDDH measurements, and the physiological properties of the novel isolate, we propose that strain SLHTYRO T should be classified as a representative of a novel species, for which the name Methanohalophilus profundi sp. nov. is proposed; the type strain is SLHTYRO T (=DSM 108854 = JCM 32768 = UBOCC-M-3308).
- Published
- 2020
- Full Text
- View/download PDF