1. Dental epithelial histo-morphogenesis in the mouse: positional information versus cell history
- Author
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Amal Nadiri, Fabienne Perrin-Schmitt, Sabine Bopp-Kuchler, Hervé Lesot, Songlin Wang, Bing Hu, Biomatériaux : Processus biophysiques et biologiques aux interfaces, Université Louis Pasteur - Strasbourg I-Faculté de Chirurgie Dentaire-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, and Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Pathology ,medicine.medical_specialty ,Mesenchyme ,Cell Culture Techniques ,Cell Separation ,Biology ,MESH: Cell Separation ,MESH: Enamel Organ ,Mesoderm ,Mice ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,medicine ,Animals ,MESH: Animals ,MESH: Mice, Inbred ICR ,MESH: Mice ,General Dentistry ,Reduced enamel epithelium ,030304 developmental biology ,MESH: Cell Culture Techniques ,MESH: Mesoderm ,Mice, Inbred ICR ,0303 health sciences ,Inner enamel epithelium ,Outer enamel epithelium ,Enamel Organ ,Enamel organ ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Epithelial Cells ,030206 dentistry ,Cell Biology ,General Medicine ,Amelogenesis ,Molar ,Enamel knot ,Cell biology ,stomatognathic diseases ,medicine.anatomical_structure ,Otorhinolaryngology ,MESH: Epithelial Cells ,Odontogenesis ,MESH: Molar ,Ameloblast ,MESH: Odontogenesis - Abstract
International audience; Reciprocal epithelial-mesenchymal interactions control odontogenesis and the cap stage tooth germ mesenchyme specifies crown morphogenesis. The aim of this work was to determine whether this mesenchyme could also control epithelial histogenesis. Dental mesenchyme and enamel organ were dissociated from mouse first lower molars at E14. At this early cap stage, the enamel organ consists of four cell types forming the inner dental epithelium (IDE), primary enamel knot (PEK), outer dental epithelium (ODE) and the stellate reticulum (SR). Pelleted trypsin-dissociated single dental epithelial cells, which had lost all positional information, were reassociated to either dental mesenchyme or dissociated mesenchymal cells and cultured in vitro. Although with different timings, teeth developed in both types of experiments showing a characteristic dental epithelial histogenesis, cusp formation, and the differentiation of functional odontoblasts and ameloblasts. The rapid progression of the initial steps of histogenesis suggested that the cell history was not memorized. The dental mesenchyme, as well as dissociated mesenchymal cells, induced the formation of a PEK indicating that no specific organisation in the mesenchyme is required for this step. However, the proportion of well-formed multicusped teeth was much higher when intact mesenchyme was used instead of dissociated mesenchymal cells. The mesenchymal cell dissociation had consequences for the functionality of the newly-formed PEK.
- Published
- 2005