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Dental Epithelial Histomorphogenesis in vitro
- Source :
- Journal of Dental Research, Journal of Dental Research, SAGE Publications (UK and US), 2005, 84 (6), pp.521-5
- Publication Year :
- 2005
- Publisher :
- HAL CCSD, 2005.
-
Abstract
- International audience; Recent developments in tooth-tissue engineering require that we understand the regulatory processes to be preserved to achieve histomorphogenesis and cell differentiation, especially for enamel tissue engineering. Using mouse first lower molars, our objectives were: (1) to determine whether the cap-stage dental mesenchyme can control dental epithelial histogenesis, (2) to test the role of the primary enamel knot (PEK) in specifying the potentialities of the dental mesenchyme, and (3) to evaluate the importance of positional information in epithelial cells. After tissue dissociation, the dental epithelium was further dissociated into individual cells, re-associated with dental mesenchyme, and cultured. Epithelial cells showed a high plasticity: Despite a complete loss of positional information, they rapidly underwent typical dental epithelial histogenesis. This was stimulated by the mesenchyme. Experiments performed at E13 demonstrated that the initial potentialities of the mesenchyme are not specified by the PEK. Positional information of dental epithelial cells does not require the memorization of their history.
- Subjects :
- 0301 basic medicine
Pathology
Cellular differentiation
Apoptosis
Cell Communication
Histogenesis
[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
MESH: Enamel Organ
Epithelium
Mesoderm
Tissue Culture Techniques
Mice
0302 clinical medicine
Tissue engineering
Image Processing, Computer-Assisted
Morphogenesis
MESH: Animals
Mice, Inbred ICR
MESH: Mesoderm
Cell Differentiation
MESH: Image Processing, Computer-Assisted
Enamel knot
medicine.anatomical_structure
MESH: Epithelial Cells
MESH: Cell Differentiation
medicine.medical_specialty
Mesenchyme
MESH: Tooth Germ
MESH: Imaging, Three-Dimensional
Biology
03 medical and health sciences
Imaging, Three-Dimensional
stomatognathic system
MESH: Cell Communication
medicine
Animals
MESH: Tissue Culture Techniques
MESH: Mice, Inbred ICR
General Dentistry
MESH: Mice
MESH: Apoptosis
Enamel Organ
Enamel organ
Tooth Germ
Epithelial Cells
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
030206 dentistry
MESH: Morphogenesis
stomatognathic diseases
MESH: Epithelium
030104 developmental biology
Subjects
Details
- Language :
- English
- ISSN :
- 00220345
- Database :
- OpenAIRE
- Journal :
- Journal of Dental Research, Journal of Dental Research, SAGE Publications (UK and US), 2005, 84 (6), pp.521-5
- Accession number :
- edsair.doi.dedup.....68a06081ac179b4f26efdbe1b09ed810