1. The structure of an E. coli tRNA f Met A 1-U 72 variant shows an unusual conformation of the A 1-U 72 base pair
- Author
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Alexey Aleksandrov, Auriane Monestier, Pierre-Damien Coureux, Yves Mechulam, Emmanuelle Schmitt, Michel Panvert, Laboratoire de Biochimie de l'Ecole polytechnique (BIOC), and École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,Stereochemistry ,Base pair ,Mutant ,MESH: Base Pairing ,MESH: Escherichia coli/genetics ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Biology ,Ribosome ,translation initiation ,03 medical and health sciences ,Eukaryotic translation ,MESH: RNA, Archaeal/chemistry ,MESH: Anticodon ,MESH: Molecular Dynamics Simulation ,Molecular Biology ,Ternary complex ,tRNA ,X-ray crystallography ,Translation (biology) ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,MESH: RNA, Bacterial/chemistry ,Acceptor ,[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] ,MESH: RNA, Transfer, Met/chemistry ,030104 developmental biology ,MESH: RNA, Bacterial/metabolism ,Transfer RNA ,MESH: RNA, Transfer, Met/metabolism ,MESH: Escherichia coli/metabolism ,MESH: Models, Molecular - Abstract
Translation initiation in eukaryotes and archaea involves a methionylated initiator tRNA delivered to the ribosome in a ternary complex with e/aIF2 and GTP. Eukaryotic and archaeal initiator tRNAs contain a highly conserved A1–U72 base pair at the top of the acceptor stem. The importance of this base pair to discriminate initiator tRNAs from elongator tRNAs has been established previously using genetics and biochemistry. However, no structural data illustrating how the A1–U72 base pair participates in the accurate selection of the initiator tRNAs by the translation initiation systems are available. Here, we describe the crystal structure of a mutant E. coli initiator tRNAfMetA1–U72, aminoacylated with methionine, in which the C1:A72 mismatch at the end of the tRNA acceptor stem has been changed to an A1–U72 base pair. Sequence alignments show that the mutant E. coli tRNA is a good mimic of archaeal initiator tRNAs. The crystal structure, determined at 2.8 Å resolution, shows that the A1–U72 pair adopts an unusual arrangement. A1 is in a syn conformation and forms a single H-bond interaction with U72. This interaction requires protonation of the N1 atom of A1. Moreover, the 5′ phosphoryl group folds back into the major groove of the acceptor stem and interacts with the N7 atom of G2. A possible role of this unusual geometry of the A1–U72 pair in the recognition of the initiator tRNA by its partners during eukaryotic and archaeal translation initiation is discussed.
- Published
- 2017