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Context-dependent anticodon recognition by class I lysyl-tRNA synthetases
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2000, 97 (26), pp.14224-8. ⟨10.1073/pnas.97.26.14224⟩
- Publication Year :
- 2000
-
Abstract
- Lysyl-tRNA synthesis is catalyzed by two unrelated families of aminoacyl-tRNA synthetases. In most bacteria and all eukarya, the known lysyl-tRNA synthetases (LysRSs) are subclass IIb-type aminoacyl-tRNA synthetases, whereas many archaea and a scattering of bacteria contain an unrelated class I-type LysRS. Examination of the recognition of partially modified tRNA Lys anticodon variants by a bacterial (from Borrelia burgdorferi ) and an archaeal (from Methanococcus maripaludis ) class I lysyl-tRNA synthetase revealed differences in the pattern of anticodon recognition between the two enzymes. U35 and U36 were both important for recognition by the B. burgdorferi enzyme, whereas only U36 played a role in recognition by M. maripaludis LysRS. Examination of the phylogenetic distribution of class I LysRSs suggested a correlation between recognition of U35 and U36 and the presence of asparaginyl-tRNA synthetase (AsnRS), which also recognizes U35 and U36 in the anticodon of tRNA Asn . However, the class II LysRS of Helicobacter pylori , an organism that lacks AsnRS, also recognizes both U35 and U36, indicating that the presence of AsnRS has solely influenced the phylogenetic distribution of class I LysRSs. These data suggest that competition between unrelated aminoacyl-tRNA synthetases for overlapping anticodon sequences is a determinant of the phylogenetic distribution of extant synthetase families. Such patterns of competition also provide a basis for the two separate horizontal gene transfer events hypothesized in the evolution of the class I lysyl-tRNA synthetases.
- Subjects :
- Lysine-tRNA Ligase
Context (language use)
Lysine—tRNA ligase
Biology
MESH: RNA, Transfer, Lys
Subclass
Borrelia burgdorferi Group
Phylogenetics
MESH: Anticodon
Anticodon
MESH: Borrelia burgdorferi Group
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
MESH: Phylogeny
Phylogeny
Genetics
Multidisciplinary
Helicobacter pylori
MESH: Lysine-tRNA Ligase
Methanococcus maripaludis
Biological Sciences
biology.organism_classification
MESH: Nucleic Acid Conformation
Transfer RNA
Horizontal gene transfer
MESH: Helicobacter pylori
bacteria
Nucleic Acid Conformation
RNA, Transfer, Lys
Archaea
Subjects
Details
- ISSN :
- 00278424 and 10916490
- Volume :
- 97
- Issue :
- 26
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....41c055e5fcb95c57d60dd6bb8c463e2d