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SUMMARY The temporal variability of polar sea ice is complex and closely linked to global climate. The amount of sea ice over an area can have a significant effect on the energies transferred between the atmosphere and the ocean. Statistics derived from sea ice observations are therefore of great interest to scientists. We showcase a new method of analysis which may be used to examine the annual variability in sea ice formation. We demonstrate our method using sea ice concentration images derived from Earth-orbiting satellites that span several decades. The growth and melt of Antarctic sea ice is modelled using a simple two parameter spatial nearest neighbour process that treats ice and water as two species competing for territory. Simulations of the model are used to estimate two time series representing rates of competition. With techniques of functional data analysis, these series may be used to detect both amplitude and phase variation, and to isolate major modes of annual variation in sea ice formation. Copyright © 2008 John Wiley & Sons, Ltd.

A chunking procedure [Bradley, P.S. and Mangasarian, O.L., 2000, Massive data discrimination via linear support vector machines. Optimization Methods and Software, 13, 1-10. Available online at: ftp://ftp.cs.wisc.edu/mathprog/tech-reports/98-05.ps] utilized in [Mangasarian, O.L. and Thompson, M.E., 2006, Massive data classification via unconstrained support vector machines. Journal of Optimization Theory and Applications, 131, 315-325. Available online at: ftp://ftp.cs.wisc.edu/pub/dmi/tech-reports/06-01.pdf] for linear classifiers is proposed here for nonlinear kernel classification of massive datasets. A highly accurate algorithm based on nonlinear support vector machines that utilize a linear programming formulation [Mangasarian, O.L., 2000, Generalized support vector machines. In: A. Smola, P. Bartlett, B. Scholkopf and D. Schuurmans (Eds) Advances in Large Margin Classifiers (Cambridge, MA: MIT Press), pp. 135-146. Available online at: ftp://ftp.cs.wisc.edu/math-prog/tech-reports/98-14.ps] is developed here as a completely unconstrained minimization problem [Mangasarian, O.L., 2005, Exact 1-Norm support vector machines via unconstrained convex differentiable minimization. Technical Report 05-03, Data Mining Institute, Computer Sciences Department, University of Wisconsin, Madison, Wisconsin. Available online at: ftp://ftp.cs.wisc.edu/pub/dmi/tech-reports/05-03.ps. Journal of Machine Learning Research, 7, 1517-1530, 2006.]. This approach together with chunking leads to a simple and accurate method for generating nonlinear classifiers for a 250,000-point dataset that typically exceeds machine capacity when standard linear programming methods such as CPLEX [ILOG, 2003, ILOG CPLEX 9.0 User's Manual, Incline Village, Nevada. Available online at: http://www.ilog.com/products/cplex/] are used. Because a 1-norm support vector machine underlies the proposed method, the approach together with a reduced support vector machine formulation [Lee, Y.-J. and Mangasarian, O.L., 2001, RSVM: reduced support vector machines. Proceedings of the First SIAM International Conference on Data Mining, Chicago, 5-7 April, CD-ROM. Available online at: ftp://ftp.cs.wisc.edu/pub/dmi/tech-reports/00-07.ps] minimizes the number of kernel functions utilized to generate a simplified nonlinear classifier.

A highly accurate algorithm, based on support vector machines formulated as linear programs (Refs. 1–2), is proposed here as a completely unconstrained minimization problem (Ref. 3). Combined with a chunking procedure (Ref. 4), this approach, which requires nothing more complex than a linear equation solver, leads to a simple and accurate method for classifying million-point datasets. Because a 1-norm support vector machine underlies the proposed approach, the method suppresses input space features as well. A state-of-the-art linear programming package (CPLEX, Ref. 5) fails to solve problems handled by the proposed algorithm.

Motivated by the fact that important real-life problems, such as the protein docking problem, can be accurately modeled by minimizing a nonconvex piecewise-quadratic function, a nonconvex underestimator is constructed as the minimum of a finite number of strictly convex quadratic functions. The nonconvex underestimator is generated by minimizing a linear function on a reverse convex region and utilizes sample points from a given complex function to be minimized. The global solution of the piecewise-quadratic underestimator is known exactly and gives an approximation to the global minimum of the original function. Successive shrinking of the initial search region to which this procedure is applied leads to fairly accurate estimates, within 0.0060%, of the global minima of synthetic nonconvex functions for which the global minima are known. Furthermore, this process can approximate a nonconvex protein docking function global minimum within four-figure relative accuracy in six refinement steps. This is less than half the number of refinement steps required by previous models such as the convex kernel underestimator (Mangasarian et al., Computational Optimization and Applications, to appear) and produces higher accuracy here.

A function on Rn with multiple local minima is approximated from below, via linear programming, by a linear combination of convex kernel functions using sample points from the given function. The resulting convex kernel underestimator is then minimized, using either a linear equation solver for a linear-quadratic kernel or by a Newton method for a Gaussian kernel, to obtain an approximation to a global minimum of the original function. Successive shrinking of the original search region to which this procedure is applied leads to fairly accurate estimates, within 0.0001% for a Gaussian kernel function, relative to global minima of synthetic nonconvex piecewise-quadratic functions for which the global minima are known exactly. Gaussian kernel underestimation improves by a factor of ten the relative error obtained using a piecewise-linear underestimator (O.L. Mangasarian, J.B. Rosen, and M.E. Thompson, Journal of Global Optimization, Volume 32, Number 1, Pages 1---9, 2005), while cutting computational time by an average factor of over 28.

On the Caribbean margins, Upper Cretaceous oceanic plateaux fragments are juxtaposed with island arc fragments of a similar age; until this study, the relationship between them was unknown. This work represents the first detailed study of one such island arc sequence, the Bonaire Washikemba Formation (BWF). These rocks display typical arc-like trace element and Hf–Nd isotopic characteristics (negative Nb and Ta anomalies, qHf of +12 to +14, qNd of +6.5 to +8). They show no indication for the involvement of oceanic plateau material in their source. This is confirmed by binary mixing hyperbolae, which indicate that the Hf–Nd isotopic composition can be modelled by mixing of

Formation of the Cretaceous Caribbean plateau, including the komatiites of Gorgona, has been linked to the currently active Gala¤pagos hotspot. We use Hf^Nd isotopes and trace element data to characterise both the Caribbean plateau and the Gala¤pagos hotspot, and to investigate the relationship between them. Four geochemical components are identified in the Gala¤pagos mantle plume: two ‘enriched’ components with OHf and ONd similar to enriched components observed in other mantle plumes, one moderately enriched component with high Nb/Y, and a fourth component which most likely represents depleted MORB source mantle. The Caribbean plateau basalt data form a linear array in Hf^Nd isotope space, consistent with mixing between two mantle components. Combined Hf^ Nd^Pb^Sr^He isotope and trace element data from this study and the literature suggest that the more enriched Caribbean end member corresponds to one or both of the enriched components identified on Gala¤pagos. Likewise, the depleted end member of the array is geochemically indistinguishable from MORB and corresponds to the depleted component of the Gala¤pagos system. Enriched basalts from Gorgona partially overlap with the Caribbean plateau array in OHf vs. ONd, whereas depleted basalts, picrites and komatiites from Gorgona have a high OHf for a given ONd, defining a high-OHf depleted end member that is not observed elsewhere within the Caribbean plateau sequences. This component is similar, however, in terms of Hf^Nd^Pb^He isotopes and trace elements to the depleted plume component recognised in basalts from Iceland and along the Reykjanes Ridge. We suggest that the Caribbean plateau represents the initial outpourings of the ancestral Gala¤pagos plume. Absence of a moderately enriched, high Nb/Y component in the older Caribbean plateau (but found today on the island of Floreana) is either due to changing source compositions of the plume over its 90 Ma history, or is an artifact of limited sampling. The high-OHf depletedcomponent sampled by the Gorgona komatiites and depleted basalts is unique to Gorgona and is not found in the Caribbean plateau. This may be an indication of the scale of heterogeneity of the Caribbean plateau system; alternatively Gorgona may represent a separate oceanic plateau derived from a completely different Pacific plume, such as the Sala y Gomez.

The preparation, photophysics, and solid state structures of octahedral organometallic Ir complexes with several different cyclometalated ligands are reported. IrCl3.nH2O cleanly cyclometalates a number of different compounds (i.e., 2-phenylpyridine, 2-(p-tolyl)pyridine, benzoquinoline, 2-phenylbenzothiazole, 2-(1-naphthyl)benzothiazole, and 2-phenylquinoline), forming the corresponding chloride-bridged dimers, CwedgeN2Ir(mu-Cl)2IrCwedgeN2 (CwedgeNis a cyclometalated ligand) in good yield. These chloride-bridged dimers react with acetyl acetone (acacH) and other bidentate, monoanionic ligands such as picolinic acid (picH) and N-methylsalicylimine (salH), to give monomeric CwedgeN2Ir(LX) complexes (LX = acac, pic, sal). The emission spectra of these complexes are largely governed by the nature of the cyclometalating ligand, leading to lambda(max) values from 510 to 606 nm for the complexes reported here. The strong spin-orbit coupling of iridium mixes the formally forbidden 3MLCT and 3pi-pi* transitions with the allowed 1MLCT, leading to a strong phosphorescence with good quantum efficiencies (0.1-0.4) and room temperature lifetimes in the microsecond regime. The emission spectra of the CwedgeN2Ir(LX) complexes are surprisingly similar to the fac-IrCwedgeN3 complex of the same ligand, even though the structures of the two complexes are markedly different. The crystal structures of two of the CwedgeN2Ir(acac) complexes (i.e., CwedgeN = ppy and tpy) have been determined. Both complexes show cis-C,C', trans-N,N' disposition of the two cyclometalated ligands, similar to the structures reported for other complexes with a "CwedgeN2Ir" fragment. NMR data (1H and 13C) support a similar structure for all of the CwedgeN2Ir(LX) complexes. Close intermolecular contacts in both (ppy)2Ir(acac) and (tpy)2Ir(acac) lead to significantly red shifted emission spectra for crystalline samples of the ppy and tpy complexes relative to their solution spectra.

Experiments in the Tokamak Fusion Test Reactor (TFTR) have explored several novel regimes of improved tokamak confinement in deuterium-tritium (D-T) plasmas, including plasmas with reduced or reversed magnetic shear in the core and high-current plasmas with increased shear in the outer region (high-l{sub i}). New techniques have also been developed to enhance the confinement in these regimes by modifying the plasma-limiter interaction through in-situ deposition of lithium. In reversed-shear plasmas, transitions to enhanced confinement have been observed at plasma currents up to 2.2 MA (q{sub a} {approx} 4.3), accompanied by the formation of internal transport barriers, where large radial gradients develop in the temperature and density profiles. Experiments have been performed to elucidate the mechanism of the barrier formation and its relationship with the magnetic configuration and with the heating characteristics. The increased stability of high-current, high-l{sub i} plasmas produced by rapid expansion of the minor cross-section, coupled with improvement in the confinement by lithium deposition has enabled the achievement of high fusion power, up to 8.7 MW, with D-T neutral beam heating. The physics of fusion alpha-particle confinement has been investigated in these regimes, including the interactions of the alphas with endogenous plasma instabilities and externally applied waves in the ion cyclotron range of frequencies. In D-T plasmas with q{sub 0} > 1 and weak magnetic shear in the central region, a toroidal Alfven eigenmode instability driven purely by the alpha particles has been observed for the first time. The interactions of energetic ions with ion Bernstein waves produced by mode-conversion from fast waves in mixed-species plasmas have been studied as a possible mechanism for transferring the energy of the alphas to fuel ions.

Linear continuous time stochastic Nash cascade conceptual models for runoff are developed. The runoff is modeled as a simple system of linear stochastic differential equations driven by white Gaussian and marked point process noises. In the case of d reservoirs, the outputs of these reservoirs form a d dimensional vector Markov process, of which only the dth coordinate process is observed, usually at a discrete sample of time points. The dth coordinate process is not Markovian. Thus runoff is a partially observed Markov process if it is modeled using the stochastic Nash cascade model. We consider how to estimate the parameters in such models. In principle, maximum likelihood estimation for the complete process parameters can be carried out directly or through some form of the EM (estimation and maximization) algorithm or variation thereof, applied to the observed process data. In this research we consider a direct approximate likelihood approach and a filtering approach to an algorithm of EM type, as developed in Thompson and Kaseke (1994). These two methods are applied to some real life runoff data from a catchment in Wales, England. We also consider a special case of the martingale estimating function approach on the runoff model in the presence of rainfall. Finally, some simulations of the runoff process are given based on the estimated parameters.

An Alfven frequency mode (AFM) is very often seen in TFTR neutral beam heated plasmas as well as in ohmic plasmas. This quasi-coherent mode has so far only been seen on magnetic fluctuation diagnostics (Mirnov coils). A close correlation between the plasma edge density and the mode activity (frequency and amplitude) has been observed, which indicates that the AFM is an edge localized mode with r/a>0.85. No direct impact of this mode on the plasma global performance or on fast ion loss (e.g., the alpha particles in DT experiments) has been observed. This mode is not the conventional TAE (toroidicity induced Alfven eigenmode). The present TAE theory cannot explain this observation. Other possible explanations are discussed

After many years of fusion research, the conditions needed for a D–T fusion reactor have been approached on the Tokamak Fusion Test Reactor (TFTR) [Fusion Technol. 21, 1324 (1992)]. For the first time the unique phenomena present in a D–T plasma are now being studied in a laboratory plasma.The first magnetic fusion experiments to study plasmas using nearly equal concentrations of deuterium and tritium have been carried out on TFTR. At present the maximum fusion power of 10.7 MW, using 39.5 MW of neutral‐beam heating, in a supershot discharge and 6.7 MW in a high‐βp discharge following a current rampdown. The fusion power density in a core of the plasma is ≊2.8 MW m−3, exceeding that expected in the International Thermonuclear Experimental Reactor (ITER) [Plasma Physics and Controlled Nuclear Fusion Research (International Atomic Energy Agency, Vienna, 1991), Vol. 3, p. 239] at 1500 MW total fusion power. The energy confinement time, τE, is observed to increase in D–T, relative to D plasmas, by 20% and the ni(0) Ti(0) τE product by 55%. The improvement in thermal confinement is caused primarily by a decrease in ion heat conductivity in both supershot and limiter‐H‐mode discharges. Extensive lithium pellet injection increased the confinement time to 0.27 s and enabled higher current operation in both supershot and high‐βp discharges. Ion cyclotron range of frequencies (ICRF) heating of a D–T plasma, using the second harmonic of tritium, has been demonstrated. First measurements of the confined alpha particles have been performed and found to be in good agreement with TRANSP [Nucl. Fusion 34, 1247 (1994)] simulations. Initial measurements of the alpha ash profile have been compared with simulations using particle transport coefficients from He gas puffing experiments. The loss of alpha particles to a detector at the bottom of the vessel is well described by the first‐orbit loss mechanism. No loss due to alpha‐particle‐driven instabilities has yet been observed. D–T experiments on TFTR will continue to explore the assumptions of the ITER design and to examine some of the physics issues associated with an advanced tokamak reactor.

Peak fusion power production of 6.2 ± 0.4 MW has been achieved in TFTR plasmas heated by deuterium and tritium neutral beams at a total power of 29.5 MW. These plasmas have an inferred central fusion alpha particle density of 1.2 x 1017 m ₋3 without the appearance of either disruptive magnetohydrodynamics events or detectable changes in Alfven wave activity. The measured loss rate of energetic alpha particles agreed with the approximately 5% losses expected from alpha particles which are born on unconfined orbits.

The final hardware modifications for tritium operation have been completed for the Tokamak Fusion Test Reactor (TFTR) [Fusion Technol. 21, 1324 (1992)]. These activities include preparation of the tritium gas handling system, installation of additional neutron shielding, conversion of the toroidal field coil cooling system from water to a FluorinertTM system, modification of the vacuum system to handle tritium, preparation, and testing of the neutral beam system for tritium operation and a final deuterium–deuterium (D–D) run to simulate expected deuterium–tritium (D–T) operation. Testing of the tritium system with low concentration tritium has successfully begun. Simulation of trace and high power D–T experiments using D–D have been performed. The physics objectives of D–T operation are production of ≊10 MW of fusion power, evaluation of confinement, and heating in deuterium–tritium plasmas, evaluation of α‐particle heating of electrons, and collective effects driven by alpha particles and testing of diag...

Nd and Hf isotope systematics of oceanic basaltic rocks are often assumed to be largely immune to the effects of hydrothermal alteration. We have tested this assumption by comparing Nd and Hf isotope data for acid-leached Cretaceous oceanic basalts from Gorgona and DSDP Leg 15 with unleached data on the same rocks. Hf isotope values and Lu/Hf ratios are relatively unaffected by leaching, but 143Nd/144Nd values of leached samples are significantly higher than those of unleached fractions of the same sample in most cases. Furthermore, the Sm/Nd ratios of the majority of leached samples are 10-40% greater than those of unleached samples. X-ray diffraction studies indicate that selective removal of secondary minerals, such as smectite, during the acid leaching process is responsible for the fractionation of Sm/Nd ratios. These results have implications for interpretation of the Hf-Nd isotope systematics of ancient submarine rocks (older than ~50 Ma), as (1) the age-corrected 143Nd/144Nd ratio may not be representative of the primary magmatic signature and (2) the uncertainty of the age-corrected IµNd value may exceed the assumed analytical precision.

The aim of this study was to identify a monoclonal antibody (MAb) suitable for use in the immunocytochemical localization of prolactin in rat tissues. We took advantage of the conservation of certain amino acid sequences in prolactin among species by examining the crossreactivity patterns of five MAb, originally generated to ovine prolactin, with rat prolactin by enzyme-linked immunoassay (ELISA), Western blot analysis, and immunocytochemistry. Two of five antibodies (17D9 and 6F11) showed reactivity with 100 ng of immobilized rat prolactin (NIH RP-3) by ELISA, 6F11 reacting more strongly than 17D9. Only 6F11 reacted with prolactin in lysates of GH4C1 rat pituitary tumor cells by Western blot analysis. When we examined the crossreactivity of the MAb with rat prolactin in monolayer cultures of GH4C1 cells by indirect immunofluorescence, we found that both 17D9 and 6F11 reacted strongly with the cultures. The distribution of staining with 17D9 or 6F11 was coincident with staining with a polyclonal antiserum to rat prolactin. Preabsorption of the antibodies with a 20-fold excess of purified rat prolactin abolished the staining of GH4C1 cell cultures with either antibody. Therefore, we have selected from a series of MAb raised to ovine prolactin two antibodies (17D9 and 6F11) that react specifically with rat prolactin in immunocytochemical studies, whereas 6F11 also reacts strongly with rat prolactin by ELISA and Western blot analysis.

Background Advising on ill-health retirement is an important role of most practising occupational physicians. In recent years, the eligibility criteria and process for gaining early retirement benefits have changed in many pension schemes in the UK. Aim To investigate the variation in rates of retirement due to ill-health in National Health Service (NHS) Trusts and Local Authorities and to update previously published guidance on ill-health retirement with specific reference to pension schemes with eligibility criteria that include permanence of incapacity due to ill-health. Methods Rates of retirement were calculated for 222 NHS Trusts and 132 Local Authorities with more than 1500 employees. Literature searches and consensus statements by the authors. Results Rates of retirement were widely distributed in the NHS Trusts and Local Authorities. The median rates of retirement were 2.11 (IQR 1.37-2.91)/1000 active members and 4.10 (IQR 3.01-6.10)/1000 employees, respectively (P < 0.001). Difficulties in the doctor-patient relationship and in ascertaining the true functional ability of some patients were identified. Conclusion There continues to be marked variation in rates of early retirement due to ill-health within and between organizations that warrants further investigation. The general and specific guidance that appears as an appendix in Supplementary data to this paper should help occupational physicians to make equitable recommendations when assessing applications for early retirement benefits and fitness to work.

A novel approach is described for the preparation of surface functionalized micro- and nanobeads using one pot synthesis by a core-shell method. Monodisperse poly(p-hydroxystyrene) is successfully prepared by grafting the p-acetoxystyrene monomer during the last 30 min of the fabrication of polystyrene bead core by emulsifier-free emulsion polymerization followed by hydrolysis of the acetoxy group by a base. The size of the resulting beads is dictated mostly by the size of the core. Hydroxyl derivatized polystyrene microspheres have been found useful as a high surface area and stable support for anchoring catalytically active silver and ruthenium nanoparticles. The bead formation, surface functionalization, and coating with metal nanoparticles have been studied using scanning electron microscopy, transmission electron microscopy, energy dispersive x-ray spectrometry, Fourier transform infrared spectrometry, and Auger analysis.

Oceanic plateaus are areas of elevated and anomalously thick oceanic crust that are believed to form by enhanced partial melting in a mantle plume that is hotter than ambient upper asthenosphere. They are regarded as the oceanic equivalent of continental flood-basalt provinces. Because of the continual subduction of oceanic crust, the oldest known oceanic plateaus occurring in situ are Cretaceous in age. In order for oceanic plateaus to be preserved in the geologic record, they must be accreted onto continental margins. This process, involving their preservation as tectonic slices, depends on the fact that oceanic plateaus are more buoyant than normal ocean floor; thus, they are not easily subducted. If these plateaus encounter an oceanic arc, subduction polarity reversal may occur, and/or the locus of subduction may step back behind the trailing edge of the advancing plateau. At a continental subduction zone, only subduction back-step occurs. Geochemical evidence shows that basaltic and picritic rocks exposed in the thickened part of the Caribbean plate and around its margins (including northern South America) are parts of an accreted oceanic plateau that originated in the Pacific Ocean during the middle-to-late Cretaceous. Cretaceous subduction-related rocks also occur around the Caribbean margins and possess geochemical signatures (e.g., lower Nb and Ti) that are distinct from those of the oceanic plateau rocks. This arc material represents the remnants of the subduction-generated rocks with which the plateau collided at 80–90 Ma. Both island arc tholeiite and calc-alkaline magmatism occurred in these Cretaceous arcs, but the changeover between the two types appears to be gradual and cannot be used to determine the timing of subduction polarity reversal. Many Cretaceous tonalitic batholiths around the Caribbean margins appear to have formed during or shortly after accretion of the plateau rocks. In addition to the arc and oceanic plateau assemblages, Jurassic to Early Cretaceous fragments of the preexisting oceanic crust also occur around the region. The environmental impact of oceanic plateau volcanism around the Cenomanian-Turonian boundary and its link to the formation of organic-rich black shales is discussed in this paper.

A Phase II trial was conducted to determine the response rate of patients with advanced renal cell carcinoma to a three-drug combination of 5-fluorouracil (5-FU), interleukin-2 (IL-2), and interferon-alpha-2b (IFN-alpha).A 2-stage accrual plan was used that was designed to determine whether response to this regimen was consistent with a true response rate of/= 30%. The regimen was comprised of 5 treatment days weekly for 4 weeks every 6 weeks. Each weekly treatment was comprised of 5-FU, 1750 mg/m(2), continuous intravenous (i.v.) infusion over 24 hours followed by IL-2, 6 MIU/m(2)/day, continuous i.v. infusion for 4 days. IFN-alpha, 6 MU/m(2), was given subcutaneously on Days 1, 2, and 5.Thirty-eight patients were entered on study, 3 of whom were ineligible. Among the 35 eligible patients there were 3 confirmed partial responses (PR) and 1 complete response (CR), for an overall response rate of 11% (95% confidence interval, 3-27%). One patient considered as having a PR had minimal evidence of residual disease and was free from disease progression at2.5 years of follow-up, as was the patient with CR. Three additional patients not qualified as having a PR were showing signs of response at the time they were removed from protocol, and another patient who was removed from protocol early for management of an infection subsequently responded to the same regimen off protocol. Thirteen patients were considered nonassessable (NASS) for response, many of whom had multiple poor risk features and were unable to complete 1 cycle of treatment.This multicenter study failed to confirm an advantageous overall response rate for this three-drug regimen. However, there were two durable responses and indications of responsiveness not scored as PRs among patients with more favorable risk factor patterns, and many poor risk NASS patients. For these reasons, the response rate reported in the current study may be a conservative reflection of the effectiveness of this regimen.

Although poor medical compliance is a major risk factor for morbidity and mortality after heart transplantation, no prospective data are available on rates of noncompliance with each component of the posttransplantation regimen. Little is known about the impact of health history, sociodemographic, or perioperative psychosocial variables on long-term compliance.Compliance in eight domains was examined in a cohort of 101 heart recipients followed through the first year after transplantation. Patients received detailed interviews at 2, 7, and 12 months after transplantation. Additional corroborative information was obtained from family member interviews and nurse evaluations. Potential predictors of noncompliance were obtained from medical record reviews and from initial patient interviews. Predictors pertained to cardiac-related history, psychiatric history, sociodemographic variables, and perioperative psychosocial status (psychologic adaptation, social supports, coping strategies).Although degree of noncompliance varied across timepoints, rates of persistent noncompliance during the year were as follows: 37% (exercise); 34% (monitoring blood pressure); 20% (medications); 19% (smoking); 18% (diet); 15% (having blood work completed); 9% (clinic attendance); and 6% (heavy drinking). Compliance in most areas worsened significantly (p0.05) over time. Background health-related and sociodemographic characteristics showed no significant influence on any area of posttransplantation compliance. Perioperative psychosocial characteristics were strong and significant predictors of noncompliance.Pretransplantation screening for background and demographic variables may have limited utility for compliance outcomes. Strategies to improve compliance should focus on psychosocial risk factors pertaining to early psychologic reactions to transplantation, the quality of family relationships, and patients' styles of coping. These risk factors are each potentially modifiable through appropriate educational and supportive interventions.

A peak fusion power production of 9.3±0.7 MW has been achieved on the Tokamak Fusion Test Reactor (TFTR) in deuterium plasmas heated by co and counter injected deuterium and tritium neutral beams with a total power of 33.7 MW. The ratio of fusion power output to heating power input is 0.27. At the time of the highest neutron flux the plasma conditions are: Te(0)=11.5 keV, Ti(0)=44 keV, ne(0)=8.5×1019 m−3, and 〈Zeff〉=2.2 giving τE=0.24 s. These conditions are similar to those found in the highest confinement deuterium plasmas. The measured D‐T neutron yield is within 7% of computer code estimates based on profile measurements and within experimental uncertainties. These plasmas have an inferred central fusion alpha fraction of 0.2% and central fusion power density of 2 MW/m3 similar to that expected in a fusion reactor. Even though the alpha velocity exceeds the Alfven velocity throughout the time of high neutron output in most high power plasmas, MHD activity is not substantially different from that in co...

SynopsisThis study examines psychological symptomatology in a cohort of 72 heart transplant recipients followed longitudinally during their first year post-transplant. In keeping with research on other domains of life stressors and illnesses, a central study goal was to identify pre-transplant and perioperative psychosocial factors associated with increased vulnerability to, and maintenance of, elevated psychological distress levels post-transplant. Average anxiety and depression levels, but not anger–hostility symptoms, were substantially elevated in the early post-transplant period, relative to normative data. Average symptom levels improved significantly over time, although one-third of the sample continued to have high distress levels at all follow-up assessments. Recipients with any of seven psychosocial characteristics at initial interview were particularly susceptible to continued high average distress levels over time: a personal history of psychiatric disorder prior to transplant; younger age; lower social support from their primary family caregiver; exposure to recent major life events involving loss; poor self-esteem; a poor sense of mastery; and use of avoidance coping strategies to manage health problems. Recipients without such factors showed improvement in average distress levels across the assessment period. These effects were stronger for anxiety than depressive symptoms, with the exception of a sizeable relationship between loss events and subsequent depression. The findings suggest that clinical interventions designed to minimize prolonged emotional distress post-transplant need to be closely tailored to heart recipients' initial psychosocial assets and liabilities.

When PC-12 cells were treated with 10 microM forskolin, the expression of two members of the granin family, secretogranin II (SgII) and chromogranin B (CgB), were differentially regulated. SgII mRNA levels declined progressively after forskolin treatment to reach a level of 22 +/- 1% of control after 48 hr, whereas CgB mRNA levels increased more rapidly, reaching a maximum of 3-fold above control after 24 hr. The dependence of these changes on an increase in cellular cAMP levels, activation of cAMP-dependent protein kinase (PKA), protein synthesis, and changes in the rate of transcription was investigated. The effects of forskolin on SgII and CgB mRNAs were reproduced by 1 mM 8-bromo-cAMP but not by 10 microM 1,9-dideoxyforskolin, an inactive analog of forskolin. The actions of forskolin on SgII and CgB mRNAs were blocked by treatment with 60 microM H-89, a selective PKA inhibitor, and were blunted in PKA-deficient PC-12 cell clones. To examine whether forskolin action was dependent on ongoing protein synthesis, PC-12 cells were treated with 1 microgram/ml cycloheximide before the addition of forskolin. The reduction in SgII mRNA levels by forskolin was not evident in PC-12 cells treated with cycloheximide. Rather, in the presence of cycloheximide, forskolin stimulated SgII mRNA levels 3.6 +/- 0.7-fold above control. The induction of CgB mRNA by forskolin was not affected by cycloheximide treatment. The superinduction of SgII mRNA by cycloheximide and forskolin was related to the extent of protein synthesis inhibition, was observed in cells treated with forskolin and other protein synthesis inhibitors, and was blunted in PKA-deficient PC-12 cells, suggesting that this effect was dependent on inhibition of protein synthesis and activation of PKA. To determine whether changes in SgII and CgB mRNA levels resulted from changes in the rate of transcription, nuclear run-on assays were performed in nuclei isolated from PC-12 cells that had been treated for 2 hr with cycloheximide, forskolin, or the two combined. Transcription of the SgII gene was not significantly affected by treatment with either forskolin or cycloheximide alone but was increased 12.9 +/- 1.0-fold above control in nuclei from cells treated with cycloheximide and forskolin together. Forskolin caused a 3.8 +/- 0.8-fold induction of CgB transcription.(ABSTRACT TRUNCATED AT 400 WORDS)

The Tokamak Fusion Test Reactor (TFTR) has performed initial high-power experiments with the plasma fueled by deuterium and tritium to nominally equal densities. Compared to pure deuterium plasmas, the energy stored in the electron and ions increased by ~20%. These increases indicate improvements in confinement associated with the use of tritium and possibly heating of electrons by α-particles.

Three isoenzyme forms (designated A, B, and C) of O-acetylserine sulfhydrylase were purified from Datura innoxia suspension cultures. Isoenzyme A is the most abundant form, comprising 45-60% of the total activity. Isoenzymes C and B comprise 35-40% and 10-20% of the activity, respectively. The specific activities of the purified isoenzymes are similar (870-893 mumol of cysteine/min/mg of protein). Molecular masses for isoenzymes A, B, and C, estimated by analytical size exclusion high performance liquid chromatography, are 63, 86, and 63 kDa, respectively. Isoenzymes A and B are homodimers; isoenzyme C is a heterodimer. Spectral analysis indicates that these isoenzymes possess a pyridoxal 5'-phosphate cofactor that binds the O-acetylserine substrate. Binding is reversible by addition of the sulfide substrate. The O-acetylserine sulfhydrylase isoenzymes are active over a broad temperature range, with maximum activity between 42 and 58 degrees C. They are active only between pH 7 and 8, with optimal activity at pH 7.6. Kinetic analysis indicates these enzymes are allosterically regulated and exhibit positive cooperativity with respect to both substrates. They are inhibited by sulfide concentrations above 200 microM. The kinetic analysis together with the physical and spectrophotometric characteristics indicate that the O-acetylserine sulfhydrylase enzymes have two active sites.

The GH4C1 pituitary tumor cell line (GH cells) serves as a model system to study the role of the granins in the packaging of PRL into secretory granules. The number of secretory granules containing PRL and two members of the granin family, chromogranin-B (CgB) and secretogranin-II (SgII), can be hormonally manipulated. In the present study we have investigated whether 1) granulogenesis in GH cells is preceded by condensation of the granins and PRL in the Golgi; 2) granulogenesis is preceded by an increase in granin expression in GH cells; and 3) PRL and the granins aggregate in vitro under high calcium, low pH conditions. GH cells were treated for up to 3 days with 17 beta-estradiol (1 nM), insulin (300 nM), and epidermal growth factor (10 nM) and were fixed in 4% paraformaldehyde for immunocytochemistry or harvested for RNA isolation and Northern blot analysis. After 1 day of hormone treatment, there was a significant increase in staining for PRL and the granins in the Golgi apparatus, which was identified using an antibody to MG-160. After 3 days of hormone treatment, PRL and granin staining was also found in a perinuclear region that was not stained with anti-MG-160 antibody, most likely representing secretory granules. An increase in PRL and granin expression contributed to increased Golgi staining, as the steady state levels of CgB, SgII, and PRL mRNA increased 186 +/- 14%, 203 +/- 7%, and 337 +/- 5% above control levels, respectively, within 6 h after hormone treatment. An in vitro aggregation system was used to determine whether PRL and the granins coprecipitate under high calcium, low pH conditions, which are thought to be characteristic of the trans-Golgi and secretory granules. Aggregation of the granins CgB and SgII was negligible during overnight dialysis against a buffer containing 150 mM NaCl and 10 mM 2[N-morpholino]ethanesulfonic acid-NaOH (pH 5.5) in the absence of calcium. There was significant aggregation of PRL under these conditions. When dialysis was performed in the presence of 10 mM CaCl2, PRL, CgB, and SgII coaggregated. This study indicates that increased expression and aggregation of the granins is associated with PRL granulogenesis in hormone-treated GH cells. However, the role of the granins may not be obligatory, as some cells can store PRL in the absence of detectable levels of CgB and SgII, and PRL has the capacity to self-aggregate.

This paper describes the effect of site of lesion on auditory detection, discrimination and speech processing. Three groups of ten patients with confirmed pathology of the eighth nerve and right and left temporal cortex and two normal-hearing control groups, differing with respect to hospitalization, participated. In each of the fifty subjects, measurements were made of detection thresholds, and difference limens for frequency and duration for 50 and 300 ms pure tones of 500 Hz and 2000 Hz. Consonant discrimination was assessed using the Four Alternative Auditory Feature Test (FosterHaggard, 1979), presented in quiet. Subjects with left temporal pathology had the largest frequency and duration difference limens. Those with either left temporal or eighth nerve pathology had significantly lower speech intelligibility scores that were correlated with the duration difference limen for short stimuli and detection thresholds, respectively. These findings challenge traditional views of cortical processing and highlight differences between peripheral and central mediators of speech processing.

The response times of three groups of subjects with pathology of the auditory pathway, localized either to the eighth nerve or to the right or left temporal cortex, were compared on three psychoacoustic tasks. These tasks included detection, frequency discrimination and duration discrimination. Measurements were made at two stimulus frequencies, 500 and 2000 Hz, in combination with two durations, 50 and 300 ms. The results were compared to those obtained for normal hospitalized and non-hospitalized control subjects. All subjects, except those with acoustic neuroma, had age-corrected normal hearing. Statistically significant group differences were apparent in discrimination but not detection, indicating the importance of task demands for response time measures, especially in patients with cortical pathology. The outcomes were not related to neuropsychological measures of speed or accuracy, supporting the conclusion that the time for decision-making rather than motor function had been affected.