Search

(Xenobiotics in food end their effects on the human organism) One of the exposure pathways to xenobiotics in humans is food intake. Most of xenobiotics are lipophilic substances cumulating in the organism, especially in the adipose tissue. If their intake is long-term and excessive, they have negative impact on human health. Eating habits, identification, analysis, and management of risks stemming from foods are important from a prevention perspective. The adipose tissue influences the distribution and especially accumulation of xenobiotics in the organism. Whether substances stored in the adipose tissue are able to affect adipogenesis and physiological functions, remains unclear. Since obesity-related dysfunctional and inflamed adipose tissue is associated with systemic inflammation, ectopic fat deposition, and multiorgan dysfunction, resulting in cardiometabolic complications of obesity; this knowledge would be immensely valuable. The study focused on the basic pathophysiological mechanisms at the level of adipocytes, specifically, the study of risk and protective factors. From preventive factors, the effect of phytoprotective substances with antioxidative potential was studied. Among the risk factors, we concentrated on the impact of persistent organic pollutants (POP's). We observed exposure to...

Psoriasis vulgaris (psoriasis) is an autoimmune disease that primarily affects the skin. The pathogenesis of psoriasis involves a combination of genetic and epigenetic modifications with a significant influence of environmental factors. The disease is characterized by papulosquamous lesions with hyperproliferation of keratinocytes, angiogenesis and dysregulation of the normal immune response leading to chronic inflammation. Excessive activation of parts of the adaptive immune system is thought to be a central factor in the pathogenesis of psoriasis. More severe forms of psoriasis tend to be associated with other diseases (comorbidities). These include metabolic and cardiovascular diseases, psoriatic arthritis, Crohn's disease and psychiatric disorders or uveitis. Many of these comorbidities show a significant association with age (the biological process of human aging). Aging is a process of gradual decline in the body's abilities and functional capabilities. It is a universal process with a considerable degree of variability that is characteristic of all higher animals. Among the theories describing aging, the theory of accumulation of damage, which integrates the previously known mechanisms of aging, is currently dominant. The theory is based on the fact that during pathophysiological processes,...

Title: Psychological Factors of Weight Loss in Patients after Bariatric Surgery Author: PhDr. Markéta Zajícová Department: Department of Psychology, Charles University in Prague, Faculty of Arts Supervisor: PhDr. Tamara Hrachovinová, PhD. Abstract: Obesity with its comorbidities has enormous impacts on the life of the individual as well as the whole society. Therefore, the attention is paid to the treatment of obesity, where bariatric surgery appears to be one of the most effective. The surgical solution offers excellent results in terms of weight reduction in the short- and medium-term period, but the increased number of weigh gain cases appears in long-term perspective. The aim of this thesis is to find out whether any of the psychological factors that are included in the psychological assessment prior to the bariatric surgery, can predict the development of weight after the surgical procedure. The research is retrospective and quantitative; it focuses on monitoring variables from psychological assessment as well as weight development from the time of the surgery to 4 years after the surgery with intervals of 3, 6, 9 months and 1, 2, 3, 4 years. Attention is focused not only on the weight itself, but also on the weight change (compared to the initial weight and the comparison between consecutive...

SELECTED NUTRIENTS IN ETIOLOGY, PREVENTION AND TREATMENT OF OBESITY The 21st century pandemic of obesity is given by to the obesitogenic environment that helps to develop a positive energy balance, where the energy intake of an individual chronically exceeds his energy need, ie energy expenditure. There is an increase in body weight by increased or abnormal body fat accumulation in the body. Preventing the development of obesity is aimed at achieving energy balance, in the case of existing overweight or obesity, the treatment lies in inducing and maintaining a negative energy balance for some time, ie stimulating energy expenditure and reducing energy intake. Selected diet determines not only energy intake, but its individual nutrients can also partially affect energy expenditure and fat tissue physiology. The aim of this work is to describe and experimentally verify some nutrients that could help in weight reduction and physiological function of adipose tissue. Based on the literature data, 19 active substances or mixtures were considered. A mixture of ω-3 polyunsaturated fatty acids (ω-3 PUFA), eicosapentaenoic acid and docosahexaenoic acid, in a daily dose of 0.6 g, in the form of fish oil in a certified food supplement was chosen as the most suitable for the experiment. In a 12-week, three-arm, parallel...

UNIVERZITA KARLOVA Lékařská fakulta v Hradci Králové Dermální expozice polycyklickým aromatickým uhlovodíkům (Vybraná rizika Goeckermanovy terapie psoriázy) Andrea Málková Autoreferát disertační práce Doktorský studijní program: Hygiena, preventivní lékařství Hradec Králové 2019 1 Summary Dermal exposure of polycyclic aromatic hydrocarbons (Selected risks of Goeckerman therapy of psoriasis) Polycyclic aromatic hydrocarbons represent a health-related group of substances commonly found in the living and working environment. Dermal form of exposure can make a very significant contribution to the overall burden on the body, and some authors estimate its proportion in severe exposures to almost half of the total absorbed dose of PAH. Contrary to this, dermal PAH exposure has so far received relatively little attention. For testing in vitro dermal penetration, the OECD recommends two models, full skin and epidermal membrane. In the presented text I present the results of an in vitro trans-epidermal penetration experiment. When penetrating selected PAHs through the epidermal membrane, we found higher Flux values and lower Lag time values compared to PAH penetration through the full skin described in the previous work. Penetration of PAHs through the epidermal membrane also showed a lower degree of data...

The prevalence of obesity has already epidemic dimensions. Recently, the obesogens have been identified as the main cause in addition to excessive food intake, the lack of physical activity and the genetic background. These substances damage the metabolic processes, interfere with the hormonal functions and impair the energy balance in behalf of gaining weight and obesity. The theoretical part of this work deals with obesity, adipose tissue, lipid droplet and obesogens. From the obesogens there is closely specified a group of persistent organic pollutants (POP) from which one representative was used in the practical part of this work. The aim of the practical part was to describe the cellular model of differentiation the mesenchymal stem cell into adipocytes and to investigate the effect of one of the most frequently occurring obesogen on the expression genes of lipid metabolism and insulin signalling pathway. The morphological changes were observed in cells during differentiation (at days 0, 4, 10 and 21). The mesenchymal cells of the elongated spindle shape changed into adipocytes filled with lipid droplets. Oil Red O staining was used for quantification of accumulated lipids. The differentiation to adipocytes was confirmed by fluorescence immunocytochemistry using a specific protein FABP4. The...

Obesity is the world's most widespread metabolic disease and its continous rise in prevalence is alarming. The severity of obesity is that it is associated with a significant increase in the risk of metabolic diseases such as type 2 diabetes mellitus, hyperlipidemia, cardiovascular disease and some types of cancer. Obesity thus contributes significantly to increasing the cost of healthcare system. Preventing the aforementioned diseases is essential to reduce this burden. This is based both on the prevention of obesity itself, on the other hand, on the knowledge and consequent influence on the mechanisms of metabolic disorders associated with obesity. These disorders are based on changes in key metabolic organs: adipose tissue, liver, skeletal muscle and pancreas. The present thesis is focused on obesity- induced disorders of adipose tissue at the metabolic, endocrine and immune levels, presented as dysfunctional adipose tissue. Special attention was paid to the detrimental effect of elevated levels of basic macronutrients (carbohydrates and lipids) and, on the other hand, to the beneficial effects of low calorie diets on molecular characteristics of adipose tissue. The acquired knowledge illuminates the mechanisms of action of nutritional stimuli on adipose tissue dysfunction at the molecular and...

This dissertation aims at verifying methodology used for somatographic evaluation of the shape and statics of the spine. The potential of the methodology verified lies in increased objectivity of upright body posture evaluation and thereby contributes to the specification of preventative measures for postural defects, and their consequences. Under a cross-sectional pilot study, a non-invasive somatographic method-a diagnostic DPT-3 system designated for spine shape diagnostics-was used to examine selected biomechanical parameters (spinal curves and statics in the sagittal plane in the upright position) in a total of 508 probands. Of this, 326 were school-age children and 182 adults. Among the key parameters monitored were: Cl (depth of cervical lordosis); LI (depth of lumbar lordosis); the sagittal balance of Cl/Ll, and VThk (distance of the peak of the thoracic kyphosis from the ideal verticals-IV). A questionnaire was used to evaluate the level of movement activity in probands and the relation between the occurrence of painful back and headaches and the measurement of biomechanical parameters and movement activity. A total of 81.3 % of children and 94.5 % of adults were found to have deviations from the biomechanically ideal posture model, which signals a postural defect. The values of the Cl (p...

(EN) GH/IGF-1 axis components (GH, growth hormone receptor (GH-R), IGF-1, IGF-1 receptor (IGF-1R), IGF-binding proteins (IGFBPs)) participate in the control of glucose metabolism, inflammatory processes as well as cell proliferation and differentiation, including adipocytes and monocytes. The aim of the present study was to evaluate the role of local mRNA expression of GH/IGF-1 axis components in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM) in the development of insulin resistance and differences of adipose tissue mass in following groups of patients: obese females with and without type 2 diabetes mellitus and subjects with active untreated acromegaly. A total number of 66 subjects were included in the study: obese females without type 2 diabetes mellitus (OB), obese females with type 2 diabetes mellitus (T2DM), acromegalic patients (AC) and healthy lean control subjects (C). T2DM underwent 2 weeks of very-low- calorie diet (VLCD - energy content 2500 kJ/day). According to our results we suggest that decreased mRNA expression of IGF-1, IGF-1R, IGFBP-2 and IGFBP-3 in adipose tissue of T2DM subjects may contribute to changes of fat differentiation capacity and the increased IGF-1R mRNA expression in peripheral monocytes in these patients may play a role in the regulation of...

4 Abstract The prevalence of obesity and its related cardiometabolic complications in children remains high across the world. Obesity is a multifactorial disease caused by interaction between genes and environmental factors. Genome-wide association studies have discovered several single nucleotide polymorphisms associated with obesity. A causal role of infection in the pathogenesis of obesity has also been considered, particularly the role of adenovirus 36 (Adv36). The aim of the Ph.D. thesis was to investigate the associations of obesity susceptibility loci (TMEM18, SH2B1, KCTD15, PCSK1, BDNF, SEC16B, MC4R, FTO) and Adv36 infection with obesity-related characteristics and complications in the Czech adolescent population. The results are described in eight publications, of which six are original papers and two are reviews. Studies were performed on a cohort of Czech adolescents recruited either from the general population (1,533 individuals from the epidemiological study) and from in-patient or outpatient weight management clinics (562 overweight/obese individuals underwent an intervention). The results demonstrated an association of TMEM18, SEC16B and FTO gene variants with obesity. Some variants of the genes involved in hypothalamic regulation of energy homeostasis − MC4R, BDNF, PCSK1 − were related to...

How far will be a weight reduction successful depends on numerous factors. We focused on genetic, nutritional, hormonal and psychobehavioral factors which may significantly affect weight loss. Among nutritional factors a special attention was paid to the role of intakes of dietary calcium and n-3 polyunsaturated fatty acids (PUFA). In subsequent studies the predictors of weight reduction. In a pilot study (study 1) the effects of dietary calcium intake in 208 overweight or obese subjects who underwent comprehensive weight management programme on weight loss were evaluated. The results of the pilot study showed that the weight loss significantly correlated with the changes in calcium intake - with increasing calcium intake higher weight loss was achieved. In the second study we investigated the effect of various forms of calcium supplementation on anthropometric, biochemical, hormonal and psychobehavioral parameters in a cohort of 67 overweight or obese perimenopausal women. We did not find any effect of calcium intake on weight loss. However, groups with calcium supplementation exhibited smaller decline in fat-free mass (FFM) compared with the placebo group. Calcium supplemented groups also demonstrated significant reductions in hunger scores. In the studies 3, 4 and 5 predictors of weight reduction were...

(teze): Úvod: Bariatrické výkony se řadí k chirurgické léčbě těžké obezity, v současné době je dokládán i jejich příznivý vliv na kompenzaci diabetu mellitu 2. typu. Metody: Shromáždili jsme data týkající se hmotnosti a kompenzace diabetu mellitu 2. typu u 45 pacientů, kteří absolvovali jeden ze tří typů bariatrických výkonů (adjustabilní gastrickou bandáž (AB), sleeve gastrektomii (SG) nebo Roux-Y gastrický bypass (RYGB)). Data jsme statisticky zpracovali a vyhodnotili jsme vývoj hmotnosti a změnu kompenzace diabetu mellitu 2. typu rok po absolvování výkonu. Výsledky: U všech typů výkonů došlo rok po provedení výkonu k poklesu hmotnosti, nejvyšší hodnota EWL (Excess Weight Loss) byla zaznamenána u sleeve gastrektomie (49,55 %), nejnižší u adjustabilní bandáže (22,846 %). Hodnota EWL u Roux-Y gastrického bypassu se blížila hodnotám dosaženým u sleeve gastrektomie (41,42 %). Porovnali jsme také redukci hmotnosti u pacientů s diabetem mellitem 2. typu a bez tohoto onemocnění - pacienti s diabetem mellitem 2. typu redukovali hmotnost méně (změna hmotnosti 20,297 kg, EWL 34,962 %) než pacienti bez diabetu mellitu 2. typu (změna hmotnosti 32,633 kg, EWL 52,873 %). U pacientů s diabetem mellitem 2. typu došlo rok po provedení výkonu ke změně hodnot glykovaného hemoglobinu - v rámci celého souboru (6,586...

Fat tissue plays a main role in the development of the metabolic syndrome. Many components of the metabolic syndrome may be improved by dietary arrangements, including an increased intake of n-3 PUFA. In addition to the positive effect of n-3 PUFA, a possible effect of a higher calcium intake on influencing weight loss and energy metabolism has also been discussed for a long time. In addition to nutritional factors, genetic factors significantly contribute to influencing weight and lipid and glucose metabolism. In study A, 40 obese women were observed during three weeks of weight reduction management. Women were randomly divided into two groups. The first group received yogurt enriched with n-3 PUFA, and the second group consumed yoghurt without the supplementation. The results show that low-dose supplementation with n-3 PUFA in yogurt in combination with a reduced energy intake increases n-3 PUFA content in serum lipids and prevents adverse changes in the composition of FA in serum after a short-term low-calorie diet. In study B, we monitored the influence of n-3 PUFA supplementation on short-term weight management with VLCD in 20 women with severe obesity, who were randomly divided into two groups. The first group received VLCD enriched with n-3 PUFA, the second group VLCD with a placebo. The addition of...

The Czech Republic is from the early 90 years, reducing rates of overweight in CBT applications, which the company operates STOB Mgr. Iva Malkova. According to available information has not been done until now no research in these courses chartedabove the influence of selected cognitive factors on changes in body weight and also to monitor the change in energy intake (behavioral factor). The aim of this study is to determine the relationship between selected cognitive factors (self-efficacy - and with respect to the general dining behavior and physical activity, self-esteem, personal causal orientation restrictive way of eating behavior) and changes in body weight. At the same time the respondents will track changes in energy intakeduring therapy. The study is to verify the reliability of the Czech version of the WELquestionnaire (Weight Efficacy Life-Style Questionnaire), PSQ (Physical Self-Efficacy Questionnaire) and extended range of restrictive behaviors related to food, based on a questionnaire TFEQ (Three Factor Eating Questionnaire).