1,390 results on '"Lymphocytic Infiltrate"'
Search Results
2. Cutaneous reactive B‐cell lymphoid proliferations.
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Khalil, Shadi, Donthi, Deepak, and Gru, Alejandro A.
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SKIN diseases , *NON-langerhans-cell histiocytosis , *PRURIGO , *HYPERPLASIA , *DRUGS - Abstract
Cutaneous lymphoid hyperplasia (CLH), also known as cutaneous pseudolymphoma, is a spectrum of benign conditions characterized by reactive B‐ and T‐cell cutaneous lymphocytic infiltrates. B‐cell lymphoid proliferations are a heterogenous group of non‐neoplastic cutaneous diseases that must be histopathologically distinguished from cutaneous B‐cell lymphomas. These proliferations can be observed as reactive phenomena to infections, medications, allergens, neoplasms, and more. Furthermore, there are many inflammatory conditions that present with reactive B‐cell infiltrates, including actinic prurigo, Zoon balanitis, Rosai‐Dorfman disease, and cutaneous plasmacytosis. This review summarizes multiple cutaneous B‐cell lymphoid proliferations within the major categories of reactive and disease‐associated CLH. Further we discuss major discriminating features of atypical CLH and malignancy. Understanding the specific patterns of B‐cell CLH is essential for the proper diagnosis and treatment of patients presenting with such lesions. [ABSTRACT FROM AUTHOR]
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- 2022
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3. The puzzle of papules over face and extrafacial areas: A rare case of disseminated idiopathic T-Cell pseudolymphoma
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Sumit A Hajare, Vaishali H Wankhade, Gitesh U Sawatkar, and Rajesh Pratap Singh
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cutaneous lymphoid hyperplasia ,lichenoid pseudovesicular papular eruption on nose ,lymphocytoma cutis ,lymphocytic infiltrate ,papules over nose ,Dermatology ,RL1-803 - Abstract
Papular lesions over face have a wide range of differential diagnoses. The clinical differentiation among these is difficult and often requires clinicopathological correlation. We hereby report the case of a 28 year old female presenting with numerous skin colored papular lesions over face, pinna and upper limbs.
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- 2021
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4. Verzögerte Lokalreaktion mit subkutaner Infiltration nach Impfung mit mRNA-1273 – ein bisher unbeschriebenes Reaktionsmuster eines COVID-Arms.
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Kofler, Lukas and Forchhammer, Stephan
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Copyright of Der Hautarzt is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2022
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5. The puzzle of papules over face and extrafacial areas: A rare case of disseminated idiopathic T-Cell pseudolymphoma.
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Hajare, Sumit, Wankhade, Vaishali, Sawatkar, Gitesh, and Singh, Rajesh
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HUMAN skin color , *PUZZLES , *DIFFERENTIAL diagnosis - Abstract
Papular lesions over face have a wide range of differential diagnoses. The clinical differentiation among these is difficult and often requires clinicopathological correlation. We hereby report the case of a 28 year old female presenting with numerous skin colored papular lesions over face, pinna and upper limbs. [ABSTRACT FROM AUTHOR]
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- 2021
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6. PD-L1 Expression in Different Segments and Histological Types of Ovarian Cancer According to Lymphocytic Infiltrate
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Ljubiša Jovanović, Radmila Janković, Andja Ćirković, Milena Jović, Tijana Janjić, Slaviša Djuričić, and Svetlana Milenković
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ovarian cancer ,PD-L1 ,lymphocytic infiltrate ,Medicine (General) ,R5-920 - Abstract
Background and Objectives: Ovarian cancer is the leading cause of death among gynecological tumors. PD-1/PD-L1 immunoregulatory mechanism is activated in ovarian cancers. Lymphocyte infiltration is a significant factor that affects its expression. We analyzed the correlation between localization of lymphocytic infiltrate and PD-L1 expression in epithelial ovarian tumors. Materials and Methods: PD-L1 expression was analyzed in 328 subjects, 122 with epithelial ovarian carcinoma, 42 with atypical proliferative tumor, and 164 with benign epithelial ovarian tumor. Expression in central and invasive tumor parts in epithelial ovarian carcinoma was combined with the most pronounced lymphocyte reaction. Immunohistochemical analysis was performed using the tissue microarray and correlated with a set of histopathology parameters. Results: PD-L1 expression was most prominent in epithelial ovarian carcinoma with different levels of expression observed between invasive and central tumor segments. A high level of PD-L1 expression on tumor cells was more frequently present in the invasive than in the central tumor parts (p < 0.001) only in high-grade serous ovarian carcinoma (HGSC). There was no significant correlation between peritumoral lymphocytic infiltrate and PD-L1 expression regardless of tumor segment. In the central tumor parts of HGSC, there was a correlation of intratumoral lymphocytic infiltrate with a higher level of PD-L1 expression (p = 0.003). Conclusions: The most prominent PD-L1 expression was observed in the invasive tumor parts of HGSC. Only the central parts of the HGSC exhibited significant PD-L1 expression in association with considerable intratumoral lymphocytic infiltrate.
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- 2021
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7. Prevalence of CD8+ cytotoxic lymphocytes in human neoplasms.
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Blessin, Niclas C., Spriestersbach, Patrick, Li, Wenchao, Mandelkow, Tim, Dum, David, Simon, Ronald, Hube-Magg, Claudia, Lutz, Florian, Viehweger, Florian, Lennartz, Maximillian, Fraune, Christoph, Nickelsen, Vera, Fehrle, Wilfried, Göbel, Cosima, Weidemann, Sören, Clauditz, Till, Lebok, Patrick, Möller, Katharina, Steurer, Stefan, and Izbicki, Jacob R.
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IMMUNE checkpoint inhibitors , *RENAL cancer , *SMALL cell lung cancer , *HODGKIN'S disease , *TUMORS , *KIDNEY pelvis - Abstract
Purpose: Immune checkpoint inhibitors have recently been approved by the US FDA as first and/or second line therapy in a subset of cancer types. Recent evidence suggests that the quantity of tumor infiltrating lymphocytes (TILs) influences the likelihood of response to immune checkpoint inhibitors. Here, we set out to assess the density of CD8+ lymphocytes in a wide range of different cancer types and subtypes. Methods: The density of CD8+ lymphocytes was compared across different cancer types using tissue microarrays (TMAs) composed of up to 50 tumor samples each from 84 different cancer types and subtypes. In total 2652 cancers and 608 normal tissues were successfully analyzed by CD8 immunohistochemistry followed by automated image analysis of digitized slides. Results: We found that the median CD8+ lymphocyte counts ranged from 6 cells/mm2 in pleomorphic adenoma up to 1573 cells/mm2 in Hodgkin's lymphoma. The CD8 counts were generally lower in normal tissues compared to cancer tissues. Blood vessels of the spleen were the only non-lymphatic tissue staining positive for CD8. Tumor types approved for checkpoint inhibitor therapy, including malignant melanoma (81), muscle invasive urothelial carcinoma (119), small cell lung cancer (120), clear cell renal cell cancer (153), squamous cell carcinoma (189) and adenocarcinoma of the lung (328) as well as Hodgkin's lymphoma (1573) were all ranking among the upper half of our list. Comparably high CD8 densities (median cells/mm2) were also found in several rare and aggressive cancer types including Merkel cell carcinoma (70), angiosarcoma (95), anaplastic thyroid cancer (156) and embryonal carcinoma of the testis (186). In 73 of the 84 analyzed cancer types, the highly variable CD8 counts occasionally exceeded the average CD8 count of tumors for which checkpoint inhibitors have been approved. Conclusion: These data support the concept that among most tumor types at least some individual cancers may benefit from treatment with immune checkpoint inhibitors. [ABSTRACT FROM AUTHOR]
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- 2020
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8. A propos d'un cas rare de panniculite lupique de diagnostic difficile au CHU Departemental Borgou/Alibori (Benin).
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Koudoukpo, Christiane, Ahomadégbé, Christelle, Ballé, Marie-Claire, Akpadjan, Fabrice, Dégboé, Bérénice, Takin, Romulus, Agbéssi, Nadège, Brun, Luc, Adégbidi, Hugues, and Atadokpèdé, Félix
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SKIN disease diagnosis , *CONSULTING firms , *PALPATION , *IMMUNOHISTOCHEMISTRY , *DIAGNOSIS - Abstract
We report an unusual case of lupus panniculitis or Kaposi-Irgang disease, of difficult diagnosis. A 40-year-old woman without particular pathological history had consulted in the Service of Dermatology of the Departemental CHU Borgou/Alibori for nodular and painful lesions of the trunk and the thoracic members having begun in the right breast and evolved for eight continuous months. The dermatological examination discovered on the trunk and the thoracic members, nodular hurts under an erythema skin, painful at the palpation; of diameters varying between one and five centimeters. Anatomical examination and immunohistochemistry helped to retain the diagnosis of lupus panniculitis and the patient was given Prednisone orally in degressive doses. Skin lesions regressed after three months. Anatomical-clinical confrontation is essential to retain a positive diagnosis of rare skin diseases such as panniculitis lupus. [ABSTRACT FROM AUTHOR]
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- 2020
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9. The effect of neoadjuvant chemotherapy on tumor-infiltrating lymphocytes in patients with breast cancer
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Ahmet Serkan Ilgun, Fatma Aktepe, Onur Gonullu, Nilgun Kapucuoglu, Kanay Yararbas, Gul Alco, Alper Ozturk, Filiz Elbuken Celebi, Zeynep Erdogan, Cetin Ordu, Caglar Unal, Tomris Duymaz, Gursel Soybir, Ekrem Yavuz, Sitki Tuzlali, Vahit Ozmen, and Tıp Fakültesi
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Cancer Research ,Lymphocytic Infiltrate ,Breast Neoplasms ,General Medicine ,Prognosis ,Immunogenicity ,Neoadjuvant Therapy ,Immune Markers ,Immune-Cell Infiltration ,Lymphocytes, Tumor-Infiltrating ,Oncology ,Stil ,Stil Change ,Breast Cancer ,Pathologic Complete Response ,Tumor Microenvironment ,Humans ,Female ,Tumor Infiltrating Lymphocyte ,Neoadjuvant Treatment - Abstract
Aim: This study investigated the effect of neoadjuvant chemotherapy (NAC) on stromal tumor-infiltrating lymphocytes (sTILs) and their treatment response. Materials & methods: 115 patients with pre-NAC core biopsies and post-NAC surgical resection specimens were reviewed. Results: There was no significant change between pre- and post-treatment sTILs. Both pre- and post-NAC sTILs were significantly lower in patients with luminal A subtype. An increase in sTILs was observed in 21 (25.9%) patients after NAC, a decrease in 29 (35.8%) and no change in 31 (38.3%; p = 0.07). Pretreatment sTIL density was independent predictor of pathological complete response in multivariate analyses (odds ratio: 1.025, 95% CI: 1.003-1.047; p = 0.023). Conclusion: High sTIL density in core biopsies was independently related to pathological complete response. In addition, ER appears to be the most crucial factor determining the rate of sTIL. New studies have shown that the tumor microenvironment is critical in tumor behavior. Immune cells surrounding tumor cells are the main components of the tumor microenvironment. Our study aimed to investigate the change in immune cells before and after chemotherapy in breast cancer patients. Our study included 115 patients. All patients underwent chemotherapy before surgery to shrink the tumor. Tru-cut biopsy pieces and the breast tissue obtained after surgery were examined. The presence of estrogen or progesterone receptors on tumor cells decreased the number of immune cells surrounding the tumor cells. The number of immune cells did not decrease after chemotherapy. Another finding was that the greater the number of immune cells around the tumor, the more likely that the tumor would disappear after chemotherapy.
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- 2022
10. 7 Year Old Male with an Enlarging, Round Area of Hair Loss
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Eng, William, Walsh, Martin J., Norman, Robert A., Series editor, and Eng, William, editor
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- 2015
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11. Inflammatory Lesions
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Chinyama, Catherine N. and Chinyama, Catherine N.
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- 2014
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12. Mucinoses
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Cockerell, Clay, Mihm, Martin C., Jr., Hall, Brian J., Chisholm, Cary, Jessup, Chad, Merola, Margaret, Cockerell, Clay, Mihm Jr., Martin C., Hall, Brian J., Chisholm, Cary, Jessup, Chad, and Merola, Margaret
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- 2014
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13. Seborrheic Keratoses
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Norman, Robert A., Young, Edward M., Jr, Norman, Robert A., and Young, Jr, Edward M.
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- 2014
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14. Epithelioid hemangioma involving large arteries in the skin
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Naomi Hardy, Linglei Ma, Michael E. Kallen, and Janina Markidan
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CD31 ,Pathology ,medicine.medical_specialty ,Histology ,integumentary system ,medicine.diagnostic_test ,business.industry ,Lumen (anatomy) ,Nodule (medicine) ,Dermatology ,Epidermal Inclusion Cyst ,Pathology and Forensic Medicine ,Lymphocytic Infiltrate ,Skin biopsy ,Medicine ,medicine.symptom ,business ,Epithelioid cell ,Epithelioid Hemangioma - Abstract
Epithelioid hemangioma (EH) is a benign vascular lesion, typically consisting of small vascular channels lined by epithelioid endothelial cells and associated with a dense lymphocytic infiltrate with eosinophils. Here, we report a rare case of EH involving large arteries. The patient presented with a nine-month history of an asymptomatic nodule on the forehead, which was thought to be an epidermal inclusion cyst. Skin biopsy revealed large arteries with clusters of epithelioid cells in the vascular walls and lumen. Scattered eosinophils were noted in the walls. Adjacent areas showed groups of small-caliber vessels lined by prominent endothelial cells and associated with a dense lymphoid infiltrate with eosinophils. No significant cytologic atypia was noted. Given the presence of the classic small vessel involvement, along with CD31 reactivity for the epithelioid cells in the large vessels, the findings are classified as EH involving large arteries, which is an uncommon subtype. There have only been a handful of such cases reported in the literature. This article is protected by copyright. All rights reserved.
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- 2021
15. Clonal cutaneous and neurosyphilis: A pitfall in pseudolymphoma diagnosis
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Shyam S Raghavan, Thomas G. Cropley, Lauren G Yi, Corina A Rusu, Enrica Marchi, Alejandro A. Gru, and Kenneth E Greer
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Pathology ,medicine.medical_specialty ,Histology ,Treponema ,biology ,medicine.diagnostic_test ,business.industry ,Dermatology ,medicine.disease ,biology.organism_classification ,Pathology and Forensic Medicine ,Lymphocytic Infiltrate ,Neurosyphilis ,medicine.anatomical_structure ,Upper trunk ,Biopsy ,Pseudolymphoma ,medicine ,Syphilis ,business ,Histiocyte - Abstract
Syphilis is a sexually transmitted infectious disease caused by the bacterium Treponema pallidum and can cause a wide variety of cutaneous manifestations, most commonly, a papulosquamous eruption of the trunk and extremities. Treatment with penicillin is curative. We report a case of a 69-year-old man who presented with recent onset of blurry vision and a non-painful, non-pruritic eruption of pink-to-violaceous dermal nodules on his upper trunk and upper extremities. Biopsies of two separate locations revealed a dense superficial and deep perivascular atypical lymphocytic infiltrate with admixed plasma cells, histiocytes, and eosinophils. Some scattered cells expressed CD30, PD1, BCL-6 and ICOS. TCR-rearrangement showed an identical TCR-gamma clone between both biopsy specimens. The patient was subsequently seen by ophthalmology and diagnosed with acute anterior uveitis. RPR was reactive and CSF studies showed findings consistent with a diagnosis of neurosyphilis. A Treponema pallidum immunostain of the skin biopsies was performed upon re-review, and was diffusely positive for spirochetes at the dermal-epidermal junction and within injured vessels. The patient was treated with penicillin G with near resolution of his skin lesions. This case highlights the unusual ability of syphilis to mimic a T-cell lymphoma with matching clones across two different biopsy sites. This article is protected by copyright. All rights reserved.
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- 2021
16. An Unusual Differential Diagnosis of Leg Ulcer
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El-Darouti, Mohammad Ali and El-Darouti, Mohammad Ali
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- 2013
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17. Adult-type dermatomyositis with secondary lymphoid follicles harbouring reactive B-cells component
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Yazid Maghrabi, Sahar Hakamy, Ahmad R. Abuzinadah, Maher Kurdi, Nizar Bahabri, and Mohammed AlSobaei
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Pathology ,medicine.medical_specialty ,Proximal muscle weakness ,Muscle biopsy ,medicine.diagnostic_test ,business.industry ,Muscle weakness ,Skeletal muscle ,Dermatomyositis ,medicine.disease ,Rash ,Lymphocytic Infiltrate ,Inflammatory myopathy ,medicine.anatomical_structure ,Neurology ,Pediatrics, Perinatology and Child Health ,medicine ,Neurology (clinical) ,medicine.symptom ,business ,Genetics (clinical) - Abstract
Dermatomyositis (DM) is an immune-mediated inflammatory disease characterized by pathognomic lesions in skin and skeletal muscle including lymphocytic infiltrates. It rarely presents with ectopic lymphoid structures, as other autoimmune and chronic inflammatory diseases. We describe a case of a 47-year-old male, who presented clinically with proximal muscle weakness, skin rash and elevated creatin kinase (CK) levels. The muscle biopsy revealed inflammatory myopathy, with perifascicular pathology, and scattered ectopic lymphoid follicles-like structures harboring reactive B-cells. Clonality analysis of B-cells using polymerase chain reaction ruled out malignant lymphoma. The patient responded favorably to steroid therapy, and his muscle weakness improved. In conclusion, the clinical and histopathologic features of DM can be atypical, and the presence of lymphoid follicles, although rare, is not inevitably linked to an unfavorable prognosis.
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- 2021
18. Features of the lymphocytic microenvironment in metastatic uveal melanoma
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0301 basic medicine ,business.industry ,medicine.medical_treatment ,Melanoma ,Immunotherapy ,medicine.disease ,Primary tumor ,Metastasis ,Lymphocytic Infiltrate ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Immune system ,Stroma ,030220 oncology & carcinogenesis ,Cancer research ,medicine ,Immunohistochemistry ,business - Abstract
Introduction.Uveal melanoma is a malignant neoplasm of the vascular tract of the eye. Prevention of metastasis of this tumor is one of the main tasks in order to increase the rates of relapse-free survival of patients. Despite the pronounced immunosuppressive activity of uveal melanoma cells, its lymphocytic microenvironment exerts its antitumor effect.Aim of the study.Compare the lymphocytic microenvironment of primary uveal melanomas and distant metastases (to the liver).Мaterials and methods.The tissue material of choroid melanoma after enucleation and the material of tumor metastases for the period 2013-2018 were studied. An immunohistochemical study was performed using CD8, CD4, and CD56 markers for the qualitative and quantitative assessment of lymphocytes in the tumor stroma.Results.Differences were found in the lymphocytic infiltration of the uveal melanoma stroma and its distant metastases. A statistically significantly greater representation of CD4, CD8-lymphocytes and CD56 cells in tumor metastases than in primary melanoma tissue samples, with CD4-lymphocytes predominant. A direct high-strength correlation was registered between the number of CD4-lymphocytes and CD8-lymphocytes.Discussion.Malignant cells actively modify their cellular and stromal-vascular environment, ensuring their active growth and reproduction. The question of the immune reactivity of the surrounding cells in relation to uveal melanoma remains debatable. According to our data, which is consistent with a number of other studies, uveal melanoma cells do not completely evade the body's immune response. Thus, the determination of possible points of antitumor exposure can be based on a detailed study of the microenvironment of uveal melanoma.Conclusions.The pronounced lymphocytic infiltrate found in uveal melanoma metastases in comparison with the primary tumor indicates an active immune response of the body to the tumor. These results of our study confirm the importance of further studying the immune-mediated antitumor effect on uveal melanoma and the need to investigate possible approaches to immunotherapy.
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- 2021
19. Evaluation of Melanocyte Loss in Mycosis Fungoides Using SOX10 Immunohistochemistry
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Cynthia Reyes Barron and Bruce R. Smoller
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Pathology ,medicine.medical_specialty ,Dermatology ,Melanocyte ,Article ,Lymphocytic Infiltrate ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,SOX10 ,medicine ,cutaneous T-cell lymphoma ,Hypopigmentation ,Mycosis fungoides ,mycosis fungoides ,business.industry ,Cutaneous T-cell lymphoma ,medicine.disease ,Lymphoma ,medicine.anatomical_structure ,RL1-803 ,030220 oncology & carcinogenesis ,Neoplastic cell ,Immunohistochemistry ,hypopigmented mycosis fungoides ,medicine.symptom ,business - Abstract
Mycosis fungoides (MF) is a subtype of primary cutaneous T-cell lymphoma (CTCL) with an indolent course that rarely progresses. Histologically, the lesions display a superficial lymphocytic infiltrate with epidermotropism of neoplastic T-cells. Hypopigmented MF is a rare variant that presents with hypopigmented lesions and is more likely to affect young patients. The etiology of the hypopigmentation is unclear. The aim of this study was to assess melanocyte loss in MF through immunohistochemistry (IHC) with SOX10. Twenty cases were evaluated, including seven of the hypopigmented subtype. The neoplastic epidermotropic infiltrate consisted predominantly of CD4+ T-cells in 65% of cases, CD8+ T-cells were present in moderate to abundant numbers in most cases. SOX10 IHC showed a decrease or focal complete loss of melanocytes in 50% of the cases. The predominant neoplastic cell type (CD4+/CD8+), age, race, gender, histologic features, and reported clinical pigmentation of the lesions were not predictive of melanocyte loss. A significant loss of melanocytes was observed in 43% of hypopigmented cases and 54% of conventional cases. Additional studies will increase our understanding of the relationship between observed pigmentation and the loss of melanocytes in MF.
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- 2021
20. Essential Criteria for Diagnosing Melanoma
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Smoller, Bruce R., Hiatt, Kim M., Smoller, Bruce R., and Hiatt, Kim M.
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- 2011
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21. Benign Melanocytic Proliferations
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Smoller, Bruce R., Hiatt, Kim M., Smoller, Bruce R., and Hiatt, Kim M.
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- 2011
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22. Lethal Midline Granuloma: An Enigma in Diagnosis—A Rare Case Report.
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Prasad, K. C., Harshitha, N., Harshita, T. R., Pillai, D. Aishwarya Raj, and Brindha, H. S.
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- 2019
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23. Immune cell infiltration, tumour budding, and the p53 expression pattern are important predictors in penile squamous cell carcinoma: a retrospective study of 152 cases.
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Hrudka J, Prouzová Z, Kendall Bártů M, Hojný J, Čapka D, Zavillová N, Matěj R, and Waldauf P
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- Male, Humans, Retrospective Studies, Tumor Suppressor Protein p53 metabolism, Prognosis, Inflammation, Carcinoma, Squamous Cell pathology, Penile Neoplasms pathology
- Abstract
Penile squamous cell carcinoma (pSCC) is a rare malignancy with a slowly increasing incidence and variable prognosis. Regional lymph node involvement signifies poor prognosis but represents a late sign, and more prognostic markers for effective patient risk stratification are urgently needed. In this retrospective study, 152 tumour samples with formalin-fixed, paraffin-embedded tissue were analysed for traditional pathological variables, tumour budding, p53, p16, and mismatch repair proteins (MMR) immunohistochemistry. The density of tumour lymphocytic infiltrate was also determined, using subjective evaluation by two pathologists (brisk/non-brisk/absent) and also using the immunoscore method, which categorised the cohort into five immunoscore groups according to the number of CD3+ and CD8+ T-cells in both the tumour centre and tumour invasion front. Only one case (0.6%) was MMR-deficient. Tumour budding count ≥5 tumour buds/20× power field and non-brisk/absent lymphocytic infiltrate were significant negative predictors of both the overall survival (OS) and cancer-specific survival (CSS), whereas a low immunoscore was a significant marker of shorter OS but not CSS. Advanced pT stage (3+4) was a significant marker of shorter CSS but not OS. In the multivariate analysis, high-grade budding was a significant parameter if adjusted for the patient's age and associated variables, except for the pN stage. The lymphocytic infiltrate retained its prognostic significance if adjusted for age and associated variables. The negative prognostic significance of the previously described parameters (lymphatic, venous, and perineural invasion, regional lymph node metastasis, and p53 mutated profile) were confirmed in our study. Grade, histological subtype, and HPV status (as determined by p16 immunohistochemistry) showed, surprisingly, little or no prognostic significance., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)
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- 2023
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24. Autoimmune Thyroid Diseases
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Khan, Ashraf, Walter, Otto, and Hunt, Jennifer L., editor
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- 2010
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25. Histologic Mimics of Cutaneous Lymphoma
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Smoller, Bruce R., Hiatt, Kim M., Smoller, Bruce R., and Hiatt, Kim M.
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- 2010
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26. Interface dermatoses
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Kempf, Werner, Hantschke, Markus, Kutzner, Heinz, and Burgdorf, Walter H. C.
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- 2008
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27. Epidermis Acantholysis
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Kempf, Werner, Hantschke, Markus, Kutzner, Heinz, and Burgdorf, Walter H. C.
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- 2008
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28. Dermis Inflammation of adnexal structures
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Kempf, Werner, Hantschke, Markus, Kutzner, Heinz, and Burgdorf, Walter H. C.
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- 2008
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29. Dermis Interstitial inflammation
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Kempf, Werner, Hantschke, Markus, Kutzner, Heinz, and Burgdorf, Walter H. C.
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- 2008
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30. Dermis Granulomatous inflammation
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Kempf, Werner, Hantschke, Markus, Kutzner, Heinz, and Burgdorf, Walter H. C.
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- 2008
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31. Oral manifestations of Hydroa vacciniforme‐like lymphoproliferative disorder: a clinicopathological study of a Peruvian population
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Thayná Melo de Lima Morais, Oslei Paes de Almeida, Ciro Dantas Soares, Javier Rendón Henao, Carla Isabelly Rodrigues-Fernandes, Felipe Paiva Fonseca, Cinthia Veronica Bardález López de Cáceres, and Wilson Delgado-Azañero
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Adult ,Male ,Herpesvirus 4, Human ,Epstein-Barr Virus Infections ,Cancer Research ,Pathology ,medicine.medical_specialty ,Proliferative index ,Population ,Hydroa vacciniforme-like lymphoproliferative disease ,complication ,Pathology and Forensic Medicine ,Lymphocytic Infiltrate ,03 medical and health sciences ,0302 clinical medicine ,Hydroa vacciniforme-like lymphoproliferative disorder ,EBV ,Peru ,medicine ,Humans ,Cytotoxic T cell ,human ,education ,lymphoproliferative disease ,education.field_of_study ,business.industry ,Herpesvirus 4 ,Epstein Barr virus ,030206 dentistry ,medicine.disease ,Lymphoproliferative Disorders ,Lymphoma ,female ,Epstein Barr virus infection ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,Hydroa Vacciniforme ,Periodontics ,Hydroa vacciniforme ,Female ,Oral Surgery ,Hydroa vacciniforme-like lymphoma ,business ,CD8 ,Rare disease - Abstract
Background Hydroa vacciniforme-like lymphoproliferative disorder (HVLPD) is a chronic Epstein-Barr virus (EBV)-positive lymphoproliferative disease which may either present as an indolent condition or progress to a systemic T-cell lymphoma. Methods All HVLPD diagnosed over a 10-year period were retrieved and clinical data regarding sex, age, oral and systemic manifestations, and clinical follow-up were obtained. Immunohistochemistry was done in order to characterize the lymphoid cells and in situ hybridization was used to demonstrate the presence of EBV. Results Eleven cases were included, with a male predominance and a mean age of 25.1 years. Buccal mucosa and the lips were the most affected oral sites, appearing as painful ulcers. All patients exhibited facial oedema, usually affecting the lips, nose, and periorbital region. The clinical course was gradual but progressive, with four patients having fever and 3 showing lymphadenopathies. All cases showed a moderate to severe lymphocytic infiltrate with angiotropism, angiocentricity and epidermotropism. Two cases affecting the lip skin exhibited a periappendageal lymphocytic infiltrate. Few large pleomorphic cells were found, surrounded by smaller and medium-sized lymphoid cells, as well as reactive plasma cells, macrophages, neutrophils, and eosinophils. All lesions exhibited a cytotoxic T cell (CD8 +) phenotype with a variable proliferative index. All cases were associated with EBV and all patients died due to complications of the disease. Conclusions HVLPD is a rare disease that may show oral involvement with a cytotoxic T-cell phenotype, and is strongly associated with EBV. As shown in this series, HVLPD may show aggressive clinical behaviour.
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- 2021
32. Carcinoma Cuniculatum of the Oral Cavity: A Series of 6 Cases and Review of Literature
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Neha Mittal, Subhash Yadav, Asawari Patil, Amit Janu, Munita Bal, and Swapnil Rane
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Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Bone Neoplasms ,Pathology and Forensic Medicine ,Lymphocytic Infiltrate ,03 medical and health sciences ,0302 clinical medicine ,Tongue ,Infiltrative Growth Pattern ,medicine ,Atypia ,Carcinoma ,Animals ,Humans ,Carcinoma, Verrucous ,Anaplasia ,Original Paper ,business.industry ,Histology ,medicine.disease ,Tongue Neoplasms ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Oral and maxillofacial surgery ,Female ,Mouth Neoplasms ,Rabbits ,Neoplasm Recurrence, Local ,medicine.symptom ,business - Abstract
Carcinoma cuniculatum (CC) is a rare variant of squamous cell carcinoma (SCC) that is characterized by minimal cytologic atypia and a unique deeply infiltrative growth pattern resembling rabbit burrows (cuniculi). With less than 75 cases reported in the head and neck, the clinical and pathologic spectrum of this entity remains poorly understood. A retrospective review of the clinical and pathologic features of archival cases of oral CC was performed. A total of six cases of oral CC were identified. Age ranged from 25-77 years; the male-to-female ratio was 5:1. All patients had a long-standing history of tobacco and betel-quid consumption. The tumors were distributed in the gingivobuccal sulcus (n = 2), the tongue (n = 2), buccal mucosa (n = 1), and the palate (n = 1). Histology in all cases typically revealed a tumor composed of well-differentiated squamous epithelium, devoid of atypia, lining deeply infiltrative, large-sized, branching, keratin-filled cavities, resembling rabbit-burrows. Dense lymphocytic infiltrates and discharging micro-abscesses were regular features. Underlying bone invasion and lymph node metastasis were observed in 1 patient. One patient with a tongue tumor developed locoregional recurrence at 10 months while none developed distant metastasis. Oral CC is a rare and under-recognized variant of SCC with locally aggressive behavior. Lack of familiarity with this variant exacerbated by the absence of cytologic anaplasia makes CC susceptible to multiple negative biopsies and erroneous diagnoses. Awareness of this clinicopathologic entity is essential to allow its accurate diagnosis and optimal management.
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- 2021
33. High-risk morphological features are less prevalent among small (<5mm) papillary thyroid microcarcinomas compared to larger (≥5mm) tumors: a study of 206 cases
- Author
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Ancuța Elena Zahan, Emőke Andrea Szász, Angela Borda, and Adela Nechifor-Boilă
- Subjects
Pathology ,medicine.medical_specialty ,Stromal cell ,Psammoma body ,business.industry ,Thyroid ,030209 endocrinology & metabolism ,medicine.disease ,Desmoplasia ,Lymphocytic Infiltrate ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Giant cell ,030220 oncology & carcinogenesis ,medicine ,General Pharmacology, Toxicology and Pharmaceutics ,medicine.symptom ,business ,General Dentistry ,Lymph node ,Calcification - Abstract
Introduction: Papillary thyroid microcarcinoma (PTMC) is defined as a PTC measuring 1 cm or less, incidentally discovered. The aim of this study was to determine whether small (Materials and methods: All consecutive PTMC cases registered at the Department of Pathology, Târgu-Mureş Emergency County Hospital between 2003-2014 were reviewed. The following have been assessed: tumor size, subcapsular versus nonsubcapsular location, extrathyroidal extension/invasion into the perithyroidal adipose tissue, multifocality, resection margins, lymph node involvement, histological variant, tumor border, stromal reaction (fibrosis/desmoplasia/sclerosis), presence of plump pink cells, nuclear features of the tumor cells, intratumoral lymphocytic infiltrate, multinucleated giant cells, psammoma bodies and stromal calcification. The cases were split in two categories: small (< 5mm) and large (≥ 5mm) PTMCs and the pathological features were evaluated in comparison. Results: Our study included 206 cases, 91 large and 115 small PTMCs, respectively. Large PTMCs were significantly associated with the presence of plump pink cells (p=0.002), well developed PTC nuclear features (p=0.003), stromal reaction (fibrosis/desmoplasia/sclerosis) (pConclusions: Our results have shown that small (
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- 2021
34. Primary Cutaneous CD4-Positive Small/Medium T-Cell Lymphoproliferative Disorder Mimicking Jessner Lymphocytic Infiltrate and Tumid Lupus—A Report of Two Cases
- Author
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Alejandro A. Gru and Lauren G. Yi
- Subjects
Adult ,CD4-Positive T-Lymphocytes ,Pathology ,medicine.medical_specialty ,Systemic lupus erythematosus ,business.industry ,T cell ,Mucin ,Dermatology ,General Medicine ,medicine.disease ,Skin Diseases ,Lymphoproliferative Disorders ,Pathology and Forensic Medicine ,Diagnosis, Differential ,Lymphocytic Infiltrate ,medicine.anatomical_structure ,Humans ,Medicine ,Female ,Differential diagnosis ,business ,Benign Lymphoproliferative Disorder ,Aged - Abstract
Primary cutaneous CD4+ small/medium T-cell lymphoproliferative disorder is a benign lymphoproliferative disorder composed of small-sized to medium-sized pleomorphic cells expressing a follicular helper T-cell phenotype. Jessner lymphocytic infiltrate and tumid lupus are cutaneous conditions characterized by the presence of rich dermal lymphocytic infiltrates with a superficial, deep, perivascular and periadnexal distribution that include copious amounts of dermal mucin deposition. We report 2 cases of primary cutaneous CD4+ small/medium T-cell lymphoproliferative disorder presenting with markedly increased dermal mucin, mimicking both Jessner lymphocytic infiltrate and tumid lupus and provide a review of the differential diagnosis and highlight key distinguishing features.
- Published
- 2021
35. Dermatological and Genital Manifestations of Lyme Disease Including Morgellons Disease
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Iris Du Cruz, Melissa C Fesler, Julie Lewis, John S Haggblad, Roberto M Martinez, Gilles A. Robichaud, Raphael B. Stricker, Marianne J Middelveen, and Jyotsna S Shah
- Subjects
Pathology ,medicine.medical_specialty ,Case Report ,Dermatology ,Lymphocytic Infiltrate ,Lesion ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Lyme disease ,Borrelia ,tickborne disease ,Medicine ,Morgellons Disease ,spirochetes ,Borrelia burgdorferi ,erythema migrans ,biology ,business.industry ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Rash ,030220 oncology & carcinogenesis ,Erythema migrans ,medicine.symptom ,business ,Morgellons disease - Abstract
Although the erythema migrans (EM) skin rash is traditionally considered a hallmark of Lyme disease, other dermatological manifestations of the tickborne disease are less well known. We describe a 49-year-old woman with erosive genital ulcerations, secondary EM rashes and jagged skin lesions associated with Lyme disease. The skin rashes exhibited fibers characteristic of Morgellons disease. Molecular testing confirmed the presence of Borrelia DNA in both vaginal culture and serum specimens. In further studies on a secondary EM lesion containing filaments, Gömöri trichrome staining revealed the presence of collagen in the filaments, while Dieterle and anti-Borrelia immunostaining revealed intracellular and extracellular Borrelia organisms. Intracellular staining for Borrelia was also observed in lymphocytic infiltrates. Lyme disease may present with a variety of genital lesions and dermatological manifestations including Morgellons disease. Careful evaluation is required to determine the presence of Borrelia organisms associated with these dermopathies.
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- 2021
36. Hydroa vacciniforme-like lymphoproliferative disorder in Ecuadorian children: A case series
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Eduardo Garzón and John Jairo Dávila-Rodríguez
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medicine.medical_specialty ,CD30 ,business.industry ,Dermatology ,medicine.disease ,medicine.disease_cause ,Epstein–Barr virus ,Cutaneous lymphoma ,Virus ,Lymphocytic Infiltrate ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,Prurigo ,hemic and lymphatic diseases ,030220 oncology & carcinogenesis ,medicine ,Hydroa vacciniforme ,business - Abstract
We report the clinical and histopathological features of hydroa vacciniforme-like lymphoproliferative disorder in five indigenous and Mestizo children. All the children resided at higher altitudes, experiencing maximal solar exposure. All cases presented with prurigo along with Epstein-Barr virus infection. Histopathologic examination showed an atypical, CD30 + lymphocytic infiltrate with angiocentricity in all, while three cases demonstrated panniculitis-like infiltrate.
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- 2021
37. Pathological thyroid findings in amiodarone-induced thyrotoxicosis
- Author
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Laura Gómez-Isaza, Noel González-Ortega, and José Cameselle-Teijeiro
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Thyroid Gland ,Amiodarone ,030209 endocrinology & metabolism ,030204 cardiovascular system & hematology ,Pathology and Forensic Medicine ,Lymphocytic Infiltrate ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,Atrial Fibrillation ,Follicular phase ,medicine ,Humans ,Pathological ,Histiocyte ,business.industry ,Thyroid ,Middle Aged ,medicine.disease ,eye diseases ,Thyrotoxicosis ,medicine.anatomical_structure ,Thyroid Epithelial Cells ,Thyroidectomy ,Thyroid function ,business ,Anti-Arrhythmia Agents ,medicine.drug - Abstract
Amiodarone (AMD) is a class III antiarrhythmic drug whose chronic or high dosage administration alters the tests of thyroid function. AMD is also associated with hypothyroidism or thyrotoxicosis. Total thyroidectomy is an efficient treatment of AMD-induced thyrotoxicosis in cases resistant to medical therapy, worsening of cardiac function and/or severe thyrotoxicosis. Although AMD is a widely used drug, its pathological consequences are not well known. We describe the pathological findings in the thyroid gland of a patient who underwent total thyroidectomy due to AMD-induced thyrotoxicosis. The surgical specimen was macroscopically normal, but histologically showed multiple follicles totally or partially invaded by clear vacuolated (foamy) histiocytes, sometimes multinucleated. Loss of thyrocytes, breaks in the follicular basal membrane and stromal fibrosis could also be appreciated but no lymphocytic infiltrates were found. An awareness of these histopathological features is particularly important for surgical pathologists, especially as there are very few published reports describing these alterations.
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- 2021
38. Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked Inheritance: Model for Autoaggression
- Author
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Ochs, Hans D., Torgerson, Troy R., Shurin, Michael R., editor, and Smolkin, Yuri S., editor
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- 2007
- Full Text
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39. NKG2D and its ligands as cytotoxic factors in cutaneous lupus erythematosus
- Author
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Gero Vorwerk, Thomas Bieber, Joerg Wenzel, and Sabine Zahn
- Subjects
Keratinocytes ,chemical and pharmacologic phenomena ,Dermatology ,Ligands ,Biochemistry ,Subacute cutaneous lupus erythematosus ,Lymphocytic Infiltrate ,Interferon ,MHC class I ,Lupus Erythematosus, Cutaneous ,medicine ,Humans ,Cytotoxic T cell ,Molecular Biology ,Innate immune system ,integumentary system ,biology ,Cytotoxins ,medicine.disease ,NKG2D ,Immunity, Innate ,Up-Regulation ,ULBP2 ,NK Cell Lectin-Like Receptor Subfamily K ,biology.protein ,Cancer research ,medicine.drug - Abstract
Cutaneous lupus erythematosus (CLE) is an autoimmune skin disorder that is characterized by an anti-epidermal lymphocytic infiltrate invading the dermo-epidermal junction, causing an interface dermatitis (ID). Pathogenesis of CLE has been linked to activation of innate immunity. NKG2D is an innate immune receptor on NK cells and distinct T-cell populations. The NKG2D ligands MHC class I polypeptide-related sequence A and B (MICA, MICB) have been associated to CLE susceptibility. Our gene microarray analyses of chronic discoid lupus erythematosus (CDLE) skin lesions, separated in epidermal, junctional and dermal skin areas via laser microdissection, revealed a high expression of NKG2D in the lymphocytic infiltrate and led us to further investigate the role of NKG2D in CLE. Pathway analyses showed a strong "interferon (IFN) signature" and vast activation of innate immune response pathways (TLR, RIG-I, cytosolic DNA sensing, JAK/STAT) in CDLE, that expressed the high NKG2D signal. Immunohistochemistry (IHC) confirmed the presence of NKG2D and its ligand MICB in CDLE and subacute cutaneous lupus erythematosus (SCLE) lesions. Finally, HaCaT cells were stimulated with nucleic acids and extracted RNA was sequenced with Illumina HiSeq and showed that stressed keratinocytes express typical NKG2D ligands MICA/B and ULBP2. This study provides first evidence that NKG2D is present in CDLE and SCLE skin lesions and could be relevant for cytotoxicity in IFN-driven skin lesions with upregulated innate immune response pathways present in CLE. It could furthermore play a role in CLE inflammation promoted by keratinocytes under cell stress.
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- 2021
40. Dermatological manifestations during COVID‐19 and histological picture: Description of two clinical cases
- Author
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Alessandra Scarabello, Silvia Mosti, Silvia Rosati, Concetta Castilletti, Fabrizio Palmieri, Eleonora Lalle, Gina Gualano, Virginia Di Bari, Laura Falasca, Roberta Nardacci, Raffaella Libertone, Maria Musso, Paola Mencarini, Franca Del Nonno, and Daniele Colombo
- Subjects
medicine.medical_specialty ,Concise Communications ,rash ,Dermatology ,Virus ,Lymphocytic Infiltrate ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Basal (phylogenetics) ,COVID-19 Testing ,0302 clinical medicine ,Immune system ,COVID‐19 ,Humans ,inflammation markers ,Medicine ,skin biopsy ,medicine.diagnostic_test ,SARS-CoV-2 ,business.industry ,Concise Communication ,COVID-19 ,General Medicine ,Exanthema ,medicine.disease ,SARS‐CoV‐2 infection ,Rash ,Pneumonia ,Erythema ,030220 oncology & carcinogenesis ,Skin biopsy ,Histopathology ,medicine.symptom ,business - Abstract
It is not yet entirely clear what is the relevance of skin symptoms and what clinical implications are related to their appearance in COVID‐19 patients. We describe two cases of COVID‐19‐associated pneumonia, which presented skin manifestations in advanced stage of illness, when nasopharyngeal swabs became negative for SARS‐CoV‐2. The first case presented erythematous, maculopapular lesions; the second developed petechial, vesicular and blood‐encrusted lesions on the limbs. Histopathology documented perivascular lymphocytic infiltrates, with prevalent CD4+ T‐cells in both patients. The research of SARS‐CoV‐2 in tissues with real time RT‐PCR was negative. Basal keratinocytes displayed C4d deposits in one case, who developed laboratory signs indicative of a procoagulative condition at the same time as the skin rash. Skin manifestations during SARS‐CoV‐2 infection seem to be clinically relevant and further studies are necessary to assess if they are linked to systemic complications, lack of viral clearance or cascades of immune responses induced by the virus, even in patients affected by mild pneumonia.
- Published
- 2021
41. Lesson to Learn From Cellular infiltrate in Scalp Biopsy of Alopecia Areata
- Author
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Fairouz K Badran, Rasha Mahmoud Genedy, Eman Tayae, and Heba Nasrallah Sabra
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Adolescent ,Alopecia Areata ,Biopsy ,Dermatology ,Pathology and Forensic Medicine ,Lymphocytic Infiltrate ,Young Adult ,Humans ,Medicine ,Scalp ,integumentary system ,medicine.diagnostic_test ,business.industry ,General Medicine ,Middle Aged ,Alopecia areata ,medicine.disease ,Cellular Infiltrate ,medicine.anatomical_structure ,Hair loss ,Vellus hair ,Female ,business ,Infiltration (medical) - Abstract
Background Alopecia areata (AA) is a common cause of hair loss. It is mediated by T lymphocytes. Scalp biopsy findings in AA differ according to the disease phase and activity. Objectives To study the cellular infiltrate in the transverse section of scalp biopsy of AA at different disease stages and in relation to disease activity. Methods The study was performed on 40 subjects with AA. A 4-mm punch biopsy was obtained from an AA scalp lesion. Biopsies were sectioned horizontally; 2 anatomical levels were studied (mid dermal and deep dermal levels). Results Ninety-five percent of AA showed noncicatrical alopecia. A significant relation was found between the course of AA and the terminal:vellus ratio. Peribulbar lymphocytic infiltration was seen in 70% of cases. Mast cells were observed in 87.5% of cases, including fibrous tract and around the arrector pili muscles. Eosinophils were detected in the scalp biopsy of 22.5% of cases. Course and activity of AA were significantly related to the peribulbar lymphocytic cell infiltration but not to mast cells and eosinophils. Conclusion Although a peribulbar lymphocytic infiltrate is the classical finding of AA, it is absent in the chronic phase. Mast cells are commonly found in the scalp biopsy of AA and could explain the potential therapeutic effect of antihistamines.
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- 2021
42. Intrauterine Transmission of SARS-CoV-2
- Author
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Jullie Anne Chiste, Laura de Almeida Lanzoni, André Luiz Permegiani de Oliveira, Cyllian Arias Fugaça, Ana Paula Percicote, Lucia de Noronha, Andrea Maciel de Oliveira Rossoni, Patricia Zadorosnei Rebutini, Daniele Margarita Marani Prá, Meri Bordignon Nogueira, Emanuele Therezinha Schueda Stonoga, and Sonia Mara Raboni
- Subjects
Pathology ,diagnosis ,Epidemiology ,Expedited ,coronavirus ,lcsh:Medicine ,medicine.disease_cause ,Umbilical cord ,Lymphocytic Infiltrate ,upper respiratory swab samples ,0302 clinical medicine ,Pregnancy ,030212 general & internal medicine ,Pregnancy Complications, Infectious ,Intrauterine Transmission of SARS-CoV-2 ,swabs ,Coronavirus ,testing ,Infectious Diseases ,medicine.anatomical_structure ,coronavirus disease ,embryonic structures ,Female ,stillbirth ,Brazil ,severe acute respiratory syndrome coronavirus 2 ,Adult ,Microbiology (medical) ,medicine.medical_specialty ,placenta ,Transplacental transmission ,030231 tropical medicine ,RT-PCR ,2019 novel coronavirus disease ,lcsh:Infectious and parasitic diseases ,respiratory infections ,03 medical and health sciences ,Fetus ,autopsy ,Placenta ,Research Letter ,medicine ,Humans ,viruses ,lcsh:RC109-216 ,Fetal Death ,saliva ,SARS-CoV-2 ,business.industry ,lcsh:R ,COVID-19 ,congenital transmission ,medicine.disease ,intrauterine transmission ,Infectious Disease Transmission, Vertical ,zoonoses ,Chronic histiocytic intervillositis ,business - Abstract
We documented fetal death associated with intrauterine transmission of severe acute respiratory syndrome coronavirus 2. We found chronic histiocytic intervillositis, maternal and fetal vascular malperfusion, microglial hyperplasia, and lymphocytic infiltrate in muscle in the placenta and fetal tissue. Placenta and umbilical cord blood tested positive for the virus by PCR, confirming transplacental transmission.
- Published
- 2021
43. Inflammatory and infectious skin diseases presenting as cutaneous pseudolymphoma
- Author
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Christina Mitteldorf
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Histology ,Systemic lupus erythematosus ,CD30 ,business.industry ,medicine.disease ,Dermatology ,3. Good health ,Pathology and Forensic Medicine ,Lymphocytic Infiltrate ,Pathogenesis ,03 medical and health sciences ,Infectious skin diseases ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Pseudolymphoma ,Medicine ,business ,Cutaneous pseudolymphoma ,Morphea - Abstract
Cutaneous pseudolymphomas are a heterogeneous group of reactive lymphocytic infiltrates, which simulates cutaneous lymphomas clinically and histologically. Although in many cases the pathogenesis is not understood, a broad spectrum of different agents inducing pseudolymphomas has been reported. Cutaneous pseudolympomas are distinguished in four main groups: (a) nodular pseudolymphomas, (b) pseudo-mycosis fungoides (pseudo-MF) and simulators of other CTCLs, (c) other pseudolymphomas and (d) an intravascular pseudolymphoma. This article focuses on pseudolymphoma entities related to infections or inflammatory skin diseases. The integration of the clinical presentation and patients’ history is mandatory for the correct diagnosis.
- Published
- 2021
44. The fungus Candida glabrata in intragastric balloon
- Author
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Prado Andressa, de Oliveira Brito Rubens, Cristina Alexandrino Becker Tania, Inez Estivalet Svidzinski Terezinha, Negri Melyssa, and da Cruz Alves Pereira Elton
- Subjects
Candida glabrata ,biology ,business.industry ,Biofilm ,Fungus ,Microbial contamination ,biology.organism_classification ,Balloon ,Microbiology ,Lymphocytic Infiltrate ,medicine.anatomical_structure ,Duodenum ,medicine ,business ,Surgical interventions - Abstract
Introduction: The relentless increase in the worldwide incidence of obesity has intensified the need for new treatments that are effective and safe. The intragastric balloon (IGB) is one such treatment that emerged. Fungal biofilms formed on medical devices and their relationship to infectious diseases is now well-established. However, studies regarding the microbial contamination suffered by this device are still incipient. Case Report: We report here the first case of an IGB affected by a Candida glabrata biofilm, characterized by microbiological and scanning electron microscopy techniques. We verified yeasts, of the same species, in the mucosa of the duodenum, which was accompanied by lymphocytic infiltrate, as revealed through histopathological investigation. Conclusion: Our findings highlight the importance of biofilm investigation on this type of device and to recognize the potential repercussions for the carrier since obese patients are immunocompromised and may undergo surgical interventions, thereby aggravating the risk of candidemia.
- Published
- 2021
45. Halo Nevus : (Nevus with halo, Sutton nevus)
- Author
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Massi, Guido, Leboit, Philip E., Massi, Guido, and Leboit, Philip E.
- Published
- 2004
- Full Text
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46. Pseudolymphomas
- Author
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Braun-Falco, Otto, Plewig, Gerd, Wolff, Helmut H., Burgdorf, Walter H. C., Braun-Falco, Otto, Plewig, Gerd, Wolff, Helmut H., and Burgdorf, Walter H. C.
- Published
- 2000
- Full Text
- View/download PDF
47. Assessment of disease activity of Graves’ ophthalmopathy
- Author
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Prummel, Mark F., Wiersinga, Wilmar M., Mourits, Maarten Ph., Prummel, Mark F., editor, Wiersinga, Wilmar M., editor, Mourits, Maarten Ph., editor, and Heufelder, Armin E., editor
- Published
- 2000
- Full Text
- View/download PDF
48. Loss of troponin‐T labelling in endomyocardial biopsies of cardiac transplant patients is associated with increased rejection grading
- Author
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H. Gibbs, David J. Crossman, Mikaiah Isaacson, and Peter Ruygrok
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Physiology ,Haematoxylin ,law.invention ,Lymphocytic Infiltrate ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Confocal microscopy ,law ,Physiology (medical) ,Biopsy ,medicine ,Grading (tumors) ,Pharmacology ,medicine.diagnostic_test ,Troponin T ,business.industry ,Histology ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Immunohistochemistry ,business - Abstract
Acute cellular rejection after cardiac transplantation surgery is routinely monitored by pathological assessment of haematoxylin and eosin (H&E) histology of endomyocardial biopsies (EMB). Unfortunately, there is considerable variation in the diagnosis of rejection that has been attributed to the subjectivity involved in assessing the degree of (a) inflammatory infiltrate and (b) myocyte damage. In this work, we sought to investigate the potential of high contrast confocal microscopy for numerically assessing inflammatory infiltrate and myocyte damage in EMB histology. Confocal microscopy was used to capture images from EMB fluorescently labelled for nuclei (DAPI), f-actin (phalloidin), troponin-T (anti-body), and extracellular matrix and cell border (wheat germ agglutinin). Images from 28 biopsy procedures were captured. Standard pathological grading of H&E histology identified the following rejection gradings: 6 0R, 16 1R, 6 2R and no 3R. Confocal imaging was able to identify equivalent features of rejection provided by H&E histology but at higher contrast facilitating quantification. Lymphocytic infiltrate was calculated as the ratio of non-myocyte nuclei to total nuclei. This metric was found to be significantly higher in the biopsies from 2R patients compared to both 1R and 0R patients (P < .05). Myocyte damage was quantified as the loss of troponin-T labelling normalised to f-actin labelling. This metric of myocyte damage found significantly lower amounts of troponin-T in the biopsies from 2R patients compared to those with a 0R rejection grading (P < .05). Confocal imaging and simple image processing routines show potential for numerically assessing both inflammatory infiltrate and myocyte damage in endomyocardial biopsy.
- Published
- 2020
49. PD-1/PD-L1 expression in anal squamous intraepithelial lesions
- Author
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Charlotte Charpentier, Francine Walker, Nathalie Gault, Emy Valette, Anne Couvelard, Margot Bucau, Laurent Abramowitz, and Nanthara Sritharan
- Subjects
PD-L1 ,Lamina propria ,Pathology ,medicine.medical_specialty ,HPV ,biology ,business.industry ,anal cancer ,immune microenvironment ,Anal dysplasia ,medicine.disease ,Lymphocytic Infiltrate ,medicine.anatomical_structure ,Oncology ,anal dysplasia ,biology.protein ,Medicine ,Immunohistochemistry ,Anal cancer ,Antibody ,business ,CD8 ,Research Paper - Abstract
Introduction Studies have shown that the PD-1/PD-L1 immunomodulatory pathway slows down anti-tumor immunity in a number of cancers. The description of the expression of these molecules has never been performed in anal low-grade/high grade squamous intra-epithelial lesions (LSIL/HSIL respectively). Materials and methods Patients followed in the AIN3 cohort were routinely sampled. For each selected sample, an immunohistochemical study was performed with anti-CD8, PD-1, PD-L1 antibodies. The presence and distribution of CD8+ lymphocytes, and the presence of PD-1+ lymphocytes and PD-L1+ epithelial cells were assessed. The comparison of these characteristics was performed between the HSIL and LSIL groups. Results 33 patients were included and 78 samples selected (60 HSIL and 18 LSIL). CD8+ lymphocytes were observed more frequently in HSIL versus LSIL in the lamina propria or intra epithelial (respectively 90% vs. 60%, p = 0.01; and 62% vs. 33%, p = 0.04). PD-1+ lymphocytes were observed more frequently in HSIL versus LSIL (41% vs 11%, p = 0.03). There was no difference between HSIL and LSIL for PD-L1+ epithelial cells. Conclusions Anal dysplastic lesions are accompanied by an inflammatory lymphocytic infiltrate expressing CD8 and PD-1, more frequent in high-grade lesions. These results highlight the involvement of the PD-1/PD-L1 pathway in the natural history of anal dysplasia.
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- 2020
50. Metastatic Melanoma Negative for 5 Melanocytic Markers, Complete Regressed Primary Cutaneous Melanoma, and Melanoma-Associated Leukoderma in the Same Patient
- Author
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Mónica García-Arpa, Claudia Ramos-Rodríguez, Ma Fernanda Relea-Calatayud, Lucía González-López, and G. Romero-Aguilera
- Subjects
medicine.medical_specialty ,Pathology ,business.industry ,Melanoma ,CD99 ,Dermatology ,General Medicine ,medicine.disease ,Pathology and Forensic Medicine ,Melanosis ,Lymphocytic Infiltrate ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Cutaneous melanoma ,medicine ,Histopathology ,business ,Melanoma-associated leukoderma ,Epithelioid cell - Abstract
Melanomas with complete histological regression have been seen very infrequently. On the other hand, the diagnosis of metastatic melanoma is based on the histopathology and positivity of markers such as S100, Melan-A, and HMB-45 whose sensitivity is 99%, 82%, and 76%, respectively. It is very rare that metastatic melanomas and even more primary melanoma are negative for all of these markers. In these rare cases, there is usually a known primary. We present the case of a 82-year-old woman with a erythematous mass in the left groin and a 1-cm black-bluish irregular nodule on the skin of the ipsilateral foot. This lesion was clinical and dermoscopically compatible with primary melanoma. In the histological evaluation of the skin, a dermis full of melanophages and hemosiderophages were found in a background of fibrosis, scarce lymphocytic infiltrate, and neovascularization. Any cells expressing melanocytic markers were observed. It was diagnosed as tumoral melanosis. Lymph nodes showed a proliferation of atypical epithelioid cells with eosinophilic cytoplasm. Mitosis was conspicuous. Tumoral cells were vimentin and CD99 positive, and S100, CD34, HMB-45, Melan-A, SOX 10, tyrosinase, C-KIT, CD45, and CKAE1/AE3 negative, and BRAF-V600 mutated was detected. During follow-up, atypical vitiligo-like lesions were discovered, suggesting the diagnosis of metastatic melanoma totally regressed in our patient.
- Published
- 2020
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