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Loss of troponināT labelling in endomyocardial biopsies of cardiac transplant patients is associated with increased rejection grading
- Source :
- Clinical and Experimental Pharmacology and Physiology. 48:137-146
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Acute cellular rejection after cardiac transplantation surgery is routinely monitored by pathological assessment of haematoxylin and eosin (H&E) histology of endomyocardial biopsies (EMB). Unfortunately, there is considerable variation in the diagnosis of rejection that has been attributed to the subjectivity involved in assessing the degree of (a) inflammatory infiltrate and (b) myocyte damage. In this work, we sought to investigate the potential of high contrast confocal microscopy for numerically assessing inflammatory infiltrate and myocyte damage in EMB histology. Confocal microscopy was used to capture images from EMB fluorescently labelled for nuclei (DAPI), f-actin (phalloidin), troponin-T (anti-body), and extracellular matrix and cell border (wheat germ agglutinin). Images from 28 biopsy procedures were captured. Standard pathological grading of H&E histology identified the following rejection gradings: 6 0R, 16 1R, 6 2R and no 3R. Confocal imaging was able to identify equivalent features of rejection provided by H&E histology but at higher contrast facilitating quantification. Lymphocytic infiltrate was calculated as the ratio of non-myocyte nuclei to total nuclei. This metric was found to be significantly higher in the biopsies from 2R patients compared to both 1R and 0R patients (P < .05). Myocyte damage was quantified as the loss of troponin-T labelling normalised to f-actin labelling. This metric of myocyte damage found significantly lower amounts of troponin-T in the biopsies from 2R patients compared to those with a 0R rejection grading (P < .05). Confocal imaging and simple image processing routines show potential for numerically assessing both inflammatory infiltrate and myocyte damage in endomyocardial biopsy.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Physiology
Haematoxylin
law.invention
Lymphocytic Infiltrate
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Confocal microscopy
law
Physiology (medical)
Biopsy
medicine
Grading (tumors)
Pharmacology
medicine.diagnostic_test
Troponin T
business.industry
Histology
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Immunohistochemistry
business
Subjects
Details
- ISSN :
- 14401681 and 03051870
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Pharmacology and Physiology
- Accession number :
- edsair.doi.dedup.....ac8336c7d1873eca2d6a50ce6c77c7fc