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Bones and teeth are biological archives, but their structure and composition are subjected to alteration overtime due to biological and chemical degradation postmortem, influenced by burial environment and conditions. Nevertheless, organic fraction preservation is mandatory for several archeometric analyses and applications. The mutual protection between biomineral and organic fractions in bones and teeth may lead to a limited diagenetic alteration, promoting a better conservation of the organic fraction. However, the correlation between elemental variations and the presence of organic materials (e.g., collagen) in the same specimen is still unclear. To fill this gap, chemiluminescent (CL) immunochemical imaging analysis has been applied for the first time for collagen localization. Then, Laser Ablation–Inductively Coupled Plasma–Mass Spectrometry (LA–ICP–MS) and CL imaging were combined to investigate the correlation between elemental (i.e., REE, U, Sr, Ba) and collagen distribution. Teeth and bones from various archeological contexts, chronological periods, and characterized by different collagen content were analyzed. Immunochemical analysis revealed a heterogeneous distribution of collagen, especially in highly degraded samples. Subsequently, LA–ICP–MS showed a correlation between the presence of uranium and rare earth elements and areas with low amount of collagen. The innovative integration between the two methods permitted to clarify the mutual relation between elemental variation and collagen preservation overtime, thus contributing to unravel the effects of diagenetic alteration in bones and teeth. Results - Bone samples. - Tooth sample. Discussion Materials and methods - Sample preparation. - Laser ablation ICP–MS analyses.

This study focuses on the analysis of bronze finds and other objects employed in metallurgical activities from the Middle and Recent Bronze Age site of Solarolo-via Ordiere (Ravenna, Italy) investigated between 2006 and 2019. Materials were found both in Middle and Recent Bronze Age layers, as well as from trenches/survey, and include mostly pins and daggers with typological affinities with other bronzes in Romagna and, more in general, in the central Po plain. Noteworthy is the presence of a golden spiral from the Middle Bronze Age 2 layers. The analysed artefacts also include metal fragments, bronze drops and implements involved in metallurgical activities, such as blowing pipes, tuyères and stone moulds, which imply casting and postcasting activities taking place at the settlement. Lead isotope analyses of 6 bronze samples designate the southern side of the Eastern Alps as preferential provenance of copper, except for one dagger constituted of copper plausibly originated from the ores of the Mitterberg area (Austria). Technological and experimental analyses suggest that the overall metallurgical production at Solarolo was not particularly complex. Objects were often re-used and re-adapted, as consequence of wear and fragmentation. Starting from the significant frequency of pins, we experimented the reproduction of two types of pins, documented in Northern Italy during these phases, namely “Cattaragna” and “con testa a rotolo” types. The experiments allowed a better understanding of the chaîne opératoire, from the production of stone moulds to the final refining of the surfaces.

A 3800 year-long radiocarbon-dated and highly-resolved palaeoecological record from Lake Fimon (N-Italy) served to investigate the effects of potential teleconnections between North Atlantic and mid-to-low latitudes at the transition from Marine Isotope Stage (MIS) 3 to 2. Boreal ecosystems documented in the Fimon record reacted in a sensitive way to millennial and sub-millennial scale Northern Hemisphere atmospheric circulation patterns. The high median time-resolution of 58 years allows the identification of five abrupt event-boundaries (i.e., main forest expansion and decline excursions) synchronous with the sharp stadial/interstadial (GS/GI) transitions within dating uncertainties. During Heinrich Stadial 3 (HS 3) we reconstruct more open and dry conditions, compared to the other GS, with a dominant regional scale fire signal. Linkages between local fires and climate-driven fuel changes resulted in high-magnitude fire peaks close to GI/GS boundaries, even exacerbated by local peatland conditions. Finally, palaeoecological data from the HS 3 interval unveiled an internal variability suggesting a peak between 30,425 and 29,772 cal BP (2σ error) which matches more depleted δ18O values in alpine speleothems. We hypothesise that this signal, broadly resembling that of other mid-latitudes proxies, may be attributed to the southward shift of the Northern Hemisphere storm tracks and the associated delayed iceberg discharge events as documented during other HS.

Fil: Menendez, Lumila Paula. Konrad Lorenz Institute; Austria. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina

Strontium isotopes in biogenic apatite, especially enamel, are widely employed to determine provenance and track migration in palaeontology and archaeology. Body tissues record the 87Sr/86Sr of bioavailable Sr of ingested food and water. To identify non-local individuals, knowledge of the 87Sr/86Sr of a non-migratory population is required. However, varying factors such as tissue turnover rates, feeding selectivity, Sr content, digestibility of food, and the ingestion of mineral dust can influence body tissue 87Sr/86Sr. To evaluate the Sr contribution of diet and water to mammalian hard tissues 87Sr/86Sr, controlled feeding studies are necessary. Here we present 87Sr/86Sr from controlled feeding experiments with two rodent species (Rattus norvegicus, Cavia porcellus). Due to the continuous and fast incremental growth of rat and guinea pig incisors (~0.1 – 0.5 mm/day), their enamel is expected to record isotopic dietary changes. For Experiment-1: Diet Switch, animals were switched from their respective supplier food to a pelleted experimental diet containing either insect-, plant-, or meat-meal and a staggered-sampling approach was used to monitor the 87Sr/86Sr changes in rat incisor enamel and bone over the course of the experiment. In Experiment-2: Basic Diets, separated cohorts (n = 6) of rats and guinea pigs were fed one of the three pelleted diets and received tap water for 54 days. While the rat incisors showed a complete tissue turnover, the slower-growing guinea pig incisors partially retained supplier diet-related isotopic compositions. In addition, one group of rats fed plant-meal pellets received Sr-rich mineral water, demonstrating that drinking water can be an important Sr source in addition to diet. Additionally, a leaching experiment showed that only a small fraction of diet-related Sr is bioavailable. Finally, in Experiment-3: Dust Addition, guinea pigs were fed pellets with and without addition of 4% of isotopically distinct dust (loess or kaolin). Animals that received kaolin-containing pellets displayed increased enamel 87Sr/86Sr. Intra-population 87Sr/86Sr variability within each feeding group was small and thus we conclude that it should not affect interpretations of 87Sr/86Sr in provenance studies. However, the differences between bulk food and leachate 87Sr/86Sr highlight the importance of Sr bioavailability for provenance studies and Sr isoscapes.

Age estimation is a fundamental aspect in juvenile osteological studies and, as such, there are many methods that rely on ontogenetic-related changes to bone morphology. The talus, being a small and compact bone, is generally well preserved in archaeological contexts, but little is known about its morphological trajectory during growth. To better nderstand this we apply a (semi)landmark-based approach to an ontogenetic sample of 26 modern human juvenile tali (known age/sex = 12; unknown age/sex = 14), grouped by 5 age categories ranging from 0 to 15 years. A template of 11 landmarks and 205 semilandmarks were applied to 26 microCT-based digital models of the juvenile tali. These were superimposed by Generalized Procrustes Analysis with the semilandmarks freely sliding against recursive updates of the Procrustes consensus. Finally, individuals of unknown age/sex were projected into the form-space determined from a Principal Component Analysis of the known sample. Our results show that most of the morphometric variation is explained by PC1 (⁓91%), which is highly correlated with size and accounts for ontogenetic allometry. Negative scores (i.e., youngest) are related to a small and globular morphology. The positive scores (i.e., oldest) account for an elongation of the talar body, which is mainly related to the development of the neck and growth of the lateral malleolar facet. Overall, our preliminary results suggest that age-related morphological variations of the talus may be used to determine the general age of juvenile skeletal remains, which could be valuable to many archaeological and forensic researchers.

This paper summarizes the current state of knowledge about the millennial scale climate variability characterizing Marine Isotope Stage 3 (MIS 3) in S-Europe and the Mediterranean area and its effects on terrestrial ecosystems. The sequence of Dansgaard-Oeschger events, as recorded by Greenland ice cores and recognizable in isotope profiles from speleothems and high-resolution palaeoecological records, led to dramatic variations in glacier extent and sea level configuration with major impacts on the physiography and vegetation patterns, both latitudinally and altitudinally. The recurrent succession of (open) woodlands, including temperate taxa, and grasslands with xerophytic elements, have been tentatively correlated to GIs in Greenland ice cores. Concerning colder phases, the Greenland Stadials (GSs) related to Heinrich events (HEs) appear to have a more pronounced effect than other GSs on woodland withdrawal and xerophytes expansion. Notably, GS 9-HE4 phase corresponds to the most severe reduction of tree cover in a number of Mediterranean records. On a long-term scale, a reduction/opening of forests throughout MIS 3 started from Greenland Interstadials (GIs) 14/13 (ca. 55–48 ka), which show a maximum in woodland density. At that time, natural environments were favourable for Anatomically Modern Humans (AMHs) to migrate from Africa into Europe as documented by industries associated with modern hominin remains in the Levant. Afterwards, a variety of early Upper Palaeolithic cultures emerged (e.g., Uluzzian and Proto-Aurignacian). In this chronostratigraphic framework, attention is paid to the Campanian Ignimbrite tephra marker, as a pivotal tool for deciphering and correlating several temporal-spatial issues crucial for understanding the interaction between AMHs and Neandertals at the time of the Middle to Upper Palaeolithic transition.

Introduction to the Special Issue "Peopling dynamics in the Mediterranean area between 45 and 39 ky ago: state of art and new data", Quaternary International, 551, 1-6 pp. in the framework of the European Research Council, ERC CoG no. 724046 SUCCESS (awarded to S. Benazzi; website: http://www.erc-success.eu).

Durante l'ultimo periodo glaciale le calotte dell'emisfero Nord (ed anche l'ice field alpino) si sono più volte espanse e contratte assumendo configurazioni diverse (Ivy-Ochs et al., 2008; Lambeck et al., 2010; Monegato et al., 2017) e raggiungendo la massima estensione intorno a 22 mila anni fa (Hughes et al., 2016) durante l'Ultimo Massimo Glaciale (UMG). A queste maggiori oscillazioni climatiche e glaciali, si sono sovrapposte variazioni climatiche a scala millenaria: i cicli Dansgaard-Oescheger (DO cycles). Tale instabilità climatica, i cui picchi caldi e freddi sono detti, rispettivamente, Greenland Interstadials (GIs) e Greenland Stadials (GSs), è documentata a scala globale. Alla scala dell'emisfero boreale, record paleoclimatici ad alta risoluzione mostrano un marcato raffreddamento durante i GSs a cui sono associati Heinrich events (HEs) (Guillevic et al., 2014; Darfeuil et al., 2016). Per meglio comprendere i meccanismi che regolano tale variabilità climatica e le risposte degli ecosistemi terrestri agli HEs, verrà preso in considerazione il record pollinico del lago di Fimon all'interno della cerchia dei Colli Berici (Vicenza), che documenta la storia ambientale della Pianura Veneta e del margine prealpino a partire dalla Penultima Glaciazione (Pini et al. 2010). Nuove indagini stratigrafiche, geocronologiche e polliniche ad alta risoluzione (media di 1 campione/80-120 anni), hanno evidenziato fasi di apertura forestale con espansione di ecosistemi di semideserto a sud delle Alpi durante il MIS 3. In particolare, fra circa 27 e 35 mila anni fa, dati biostratigrafici e datazioni radiocarboniche restituiscono un robusto quadro cronologico per l'intervallo che precede, documenta e segue il GS 5.1-HE3 (30,6- 28,9 mila anni fa, cronologia GICC05, Rasmussen et al., 2014). Pinete dominate da Pinus sylvestris con betulla erano diffuse fra 35-30 mila anni fa nel settore prealpino sud-orientale. Il lago di Fimon era un bacino con acque poco profonde soggetto a oscillazioni del livello lacustre fino a 25505±99 anni fa (età 14C BP, median probability: 29599 anni cal BP), quando si sviluppa una torbiera a Cyperaceae che si mantiene per circa 2500 anni. All'inizio di questa fase, con un cambio centrato a circa 29,5 mila anni fa, le foreste subiscono una brusca contrazione (percentuali polliniche della componente arborea si riducono dal 70 al 30%), accompagnata dalla riduzione del segnale degli incendi a scala locale. Si espandono praterie (Gramineae, Asteraceae, Cichorioideae) con arbusti xerici e piante erbacee di ambiente steppico (Hippophae, Artemisia, Chenopodiaceae, Ephedra). Tale evento, di durata millenaria, può essere correlato alla fase freddo-arida dell'HE3, visibile in altri record pollinici continui in Nord Italia (Azzano decimo; Pini et al., 2009) e area mediterranea (Lago Grande di Monticchio, Tenaghi Philippon; Wulf et al., 2018). La fase successiva: interstadiali GI 4 e GI 3 (rispettivamente 28,9 e 27,78 mila anni fa, cronologia GICC05, Rasmussen et al., 2014), è caratterizzata da una nuova espansione di pinete con larice, betulla e ginepro, e dall'intensificazione di incendi a scala locale.

Archaeological information and paleoanthropological analysis align both teeth to Neanderthal. These results point out that the Broion and Roccia San Sebastiano teeth represent, along with the deciduous tooth Cavallo D (from the final Mousterian deposit of Grotta del Cavallo) [5], the most recent Neanderthal remains in Italy currently known.

We obtained high-resolution elemental maps (pixel size ranging between 10 and 20 ?m) of the dental crown. - The two Roman teeth showed clear Pb variation through the crown, highly correlated with enamel growth trajectories, likely linked to lead environmental exposure during the individual's lifetime. - Individual SCR313: Pb peak at ~410 days, visible in both dentine and enamel. - Individual SCR249: low pre-natal Pb levels, that increase post-natally; a Pb peak coincides with the neonatal line. - We identified several areas in both enamel and dentine affected by post-depositional processes.

Human osteological samples (n = 23) taken from different anatomical parts of 11 individuals from the early modern (16th–18th century AD) site of Roccapelago (Modena, Italy) were systematically analysed for δ13C, δ15N and trace elements to investigate their diet. δ13C and δ15N correlate and show a high variability between individuals, attesting to the dietary contribution of C4 plants. This is supported by pollen analysis of the burial site samples, which revealed the presence of maize. δ15N correlates with Sr/Ca, suggesting that the main protein source could have been milk and dairy. We therefore interpret the strong correlation between δ13C and δ15N as evidence for C4-plant foraging practice and the exploitation of livestock for meat and milk, combined with possible direct intake of C4 plants. The Roccapelago site represents an important case study to track the evolution of the post-medieval diet and the introduction of maize cultivation in southern Europe, as also attested by historical sources.

During the Upper Palaeolithic animal teeth were consistently used by Anatomically Modern Humans as ornamental and symbolic objects used to facilitate individual and group-level interaction. Two key Northeastern Italian sites, "Grotta di Fumane" (VR) and "Grotta del Broion" (VI) show that Cervus elaphus teeth were often used for this purpose. While Grotta di Fumane yielded evidence of red deer incisors with groovings on the root, Grotta del Broion testifies to the use of deer canines and a completely different techonological approach based on preparatory scraping and perforation by rotation. An in-detail description of these processes thanks to stereomicroscope and 3D digital microscope allowed us to uncover a pattern of regional variabiliy in tooth choice and alteration practices that may be linked to the adaptive strategies of Aurignacian and Gravettian hunter-gatherers of Northern Italy as well as to the emergence of a symbolic behaviour which is deeply rooteed in human culture.

The interaction of beta-amyloid (Abeta) peptides with externally applied electric fields (EF) of varying strengths was investigated by means of molecular dynamics (MD) simulations. The results suggest that the EF favors the switch of Abeta-peptides from helical to beta-sheet conformation, and a mechanism is tentatively proposed. Switching off the field does not restore the original conformation.