108 results on '"Lucia Anemona"'
Search Results
2. Small vascular lesions of the breast diagnosed by magnetic resonance imaging‐guided vacuum assisted biopsy: Report of 2 cases
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Tommaso Perretta, MD, PhD, Rosaria Meucci, MD, Maria Lina Serio, MD, Aurelia Caliandro, MD, Michela Censi, MD, Emanuela Beninati, MD, Alberto Collura, MD, Gianluca Vanni, MD, PhD, Erica Giacobbi, MD, Lucia Anemona, MD, PhD, and Chiara Adriana Pistolese, MD, PhD
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Vascular breast lesions ,Magnetic resonance imaging ,MRI guided vacuum assisted breast biopsy ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Vascular lesions of the breast comprise a heterogeneous group that includes a variety of benign, atypical, and malignant lesions. These are a diagnostic challenge given variable clinical, radiological and pathological presentation, especially when they are small and asymptomatic. We present 2 cases of these rare lesions of the breast which were occult to mammographics and ultrasound studies. Both the lesions were detected only on magnetic resonance imaging, most helpful in the diagnosis of these rare tumor. Histopathological examinations following the magnetic resonance guided biopsies, were initially interpreted as negative for breast cancer in both cases. These turned out to be respectively a low grade angiosarcoma and a benign vascular lesion after a new histopathological examination following a larger magnetic resonance guided biopsies performed in light of the radiology-pathology discordance. Although rare, it is important to consider vascular tumours of the breast; radiologists need to be aware such tumors may present non-specific imaging features.
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- 2022
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3. Aggressive Primary Cutaneous Anaplastic T-Cell Lymphoma Successfully Treated with Autologous Stem Cell Transplant and Brentuximab Vedotin Consolidation: Case Report and Review of the Literature
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Luca Guarnera, Federico Meconi, Marco Pocci, Fabiana Esposito, Manuela Rizzo, Vito Mario Rapisarda, Annagiulia Zizzari, Cosimo Di Raimondo, Livio Pupo, Lucia Anemona, and Maria Cantonetti
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cutaneous lymphoma ,anaplastic T-cell lymphoma ,autologous stem cell transplant ,brentuximab vedotin ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Primary cutaneous CD30+ lymphoproliferative disorders include primary cutaneous anaplastic large cell lymphoma (pcALCL) and lymphomatoid papulosis. The prognosis of the disease is usually excellent but, in a minority of cases, it presents with extracutaneous involvement and aggressive behavior. The case we present—relapsed after surgical excision, immunosuppressive therapy, and conventional chemotherapy—is the first one treated with Autologous Stem Cell transplant followed by Brentuximab Vedotin consolidation, a scheme already used for high risk Hodgkin Lymphoma.
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- 2022
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4. In Vivo Identification of H3K9me2/H3K79me3 as an Epigenetic Barrier to Carcinogenesis
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Maria Cristina Piro, Valeria Gasperi, Alessandro De Stefano, Lucia Anemona, Claudio Raffaele Cenciarelli, Manuela Montanaro, Alessandro Mauriello, Maria Valeria Catani, Alessandro Terrinoni, and Alessandra Gambacurta
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epigenetics ,chromatin ,histone modifier enzymes ,H3 methylation ,cell reprogramming ,cancer ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The highly dynamic nature of chromatin’s structure, due to the epigenetic alterations of histones and DNA, controls cellular plasticity and allows the rewiring of the epigenetic landscape required for either cell differentiation or cell (re)programming. To dissect the epigenetic switch enabling the programming of a cancer cell, we carried out wide genome analysis of Histone 3 (H3) modifications during osteogenic differentiation of SH-SY5Y neuroblastoma cells. The most significant modifications concerned H3K27me2/3, H3K9me2, H3K79me1/2, and H3K4me1 that specify the process of healthy adult stem cell differentiation. Next, we translated these findings in vivo, assessing H3K27, H3K9, and H3K79 methylation states in biopsies derived from patients affected by basalioma, head and neck carcinoma, and bladder tumors. Interestingly, we found a drastic decrease in H3K9me2 and H3K79me3 in cancer specimens with respect to their healthy counterparts and also a positive correlation between these two epigenetic flags in all three tumors. Therefore, we suggest that elevated global levels of H3K9me2 and H3K79me3, present in normal differentiated cells but lost in malignancy, may reflect an important epigenetic barrier to tumorigenesis. This suggestion is further corroborated, at least in part, by the deranged expression of the most relevant H3 modifier enzymes, as revealed by bioinformatic analysis. Overall, our study indicates that the simultaneous occurrence of H3K9me2 and H3K79me3 is fundamental to ensure the integrity of differentiated tissues and, thus, their combined evaluation may represent a novel diagnostic marker and potential therapeutic target.
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- 2023
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5. The risk of carotid plaque instability in patients with metabolic syndrome is higher in women with hypertriglyceridemia
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Francesca Servadei, Lucia Anemona, Marina Cardellini, Manuel Scimeca, Manuela Montanaro, Valentina Rovella, Francesca Di Daniele, Erica Giacobbi, Iacopo Maria Legramante, Annalisa Noce, Rita Bonfiglio, Patrizia Borboni, Nicola Di Daniele, Arnaldo Ippoliti, Massimo Federici, and Alessandro Mauriello
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Metabolic syndrome ,Carotid ,Histology ,Hypertriglyceridemia ,Post‐menopause ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background Metabolic syndrome certainly favors growth of carotid plaque; however, it is uncertain if it determines plaque destabilization. Furthermore, it is likely that only some components of metabolic syndrome are associated with increased risk of plaque destabilization. Therefore, we evaluated the effect of different elements of metabolic syndrome, individually and in association, on carotid plaques destabilization. Methods A total of 186 carotid endarterectomies from symptomatic and asymptomatic patients were histologically analysed and correlated with major cardiovascular risk factors. Results Metabolic syndrome, regardless of the cluster of its components, is not associated with a significant increase in risk of plaque destabilization, rather with the presence of stable plaques. The incidence of unstable plaques in patients with metabolic syndrome is quite low (43.9 %), when compared with that seen in the presence of some risk factors, but significantly increases in the subgroup of female patients with hypertriglyceridemia, showing an odds ratio of 3.01 (95% CI, 0.25–36.30). Conclusions Our data may help to identify patients with real increased risk of acute cerebrovascular diseases thus supporting the hypothesis that the control of hypertriglyceridemia should be a key point on prevention of carotid atherosclerotic plaque destabilization, especially in post-menopausal female patients.
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- 2021
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6. ZNF750: A Novel Prognostic Biomarker in Metastatic Prostate Cancer
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Manuela Montanaro, Massimiliano Agostini, Lucia Anemona, Elena Bonanno, Francesca Servadei, Enrico Finazzi Agrò, Anastasios D. Asimakopoulos, Carlo Ganini, Chiara Cipriani, Marta Signoretti, Pierluigi Bove, Francesco Rugolo, Benedetta Imperiali, Gerry Melino, Alessandro Mauriello, and Manuel Scimeca
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ZNF750 ,prostate cancer ,metastasis ,prognostic biomarker ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Prostate cancer is the most frequently diagnosed cancer and the fifth leading cause of cancer death among men in 2020. The clinical decision making for prostate cancer patients is based on the stratification of the patients according to both clinical and pathological parameters such as Gleason score and prostate-specific antigen levels. However, these tools still do not adequately predict patient outcome. The aim of this study was to investigate whether ZNF750 could have a role in better stratifying patients, identifying those with a higher risk of metastasis and with the poorest prognosis. The data reported here revealed that ZNF750 protein levels are reduced in human prostate cancer samples, and this reduction is even higher in metastatic samples. Interestingly, nuclear positivity is significantly reduced in patients with metastatic prostate cancer, regardless of both Gleason score and grade group. More importantly, the bioinformatics analysis indicates that ZNF750 expression is positively correlated with better prognosis. Overall, our findings suggest that nuclear expression of ZNF750 may be a reliable prognostic biomarker for metastatic prostate cancer, which lays the foundation for the development of new biological therapies.
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- 2023
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7. The Combination of Immune Checkpoint Blockade with Tumor Vessel Normalization as a Promising Therapeutic Strategy for Breast Cancer: An Overview of Preclinical and Clinical Studies
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Ombretta Melaiu, Gianluca Vanni, Ilaria Portarena, Chiara Adriana Pistolese, Lucia Anemona, Silvia Pomella, Roberto Bei, Oreste Claudio Buonomo, Mario Roselli, Alessandro Mauriello, and Giovanni Barillari
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breast cancer ,angiogenesis ,vessel normalization ,tumor microenvironment ,immunosuppression ,antitumor immunity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Immune checkpoint inhibitors (ICIs) have a modest clinical activity when administered as monotherapy against breast cancer (BC), the most common malignancy in women. Novel combinatorial strategies are currently being investigated to overcome resistance to ICIs and promote antitumor immune responses in a greater proportion of BC patients. Recent studies have shown that the BC abnormal vasculature is associated with immune suppression in patients, and hampers both drug delivery and immune effector cell trafficking to tumor nests. Thus, strategies directed at normalizing (i.e., at remodeling and stabilizing) the immature, abnormal tumor vessels are receiving much attention. In particular, the combination of ICIs with tumor vessel normalizing agents is thought to hold great promise for the treatment of BC patients. Indeed, a compelling body of evidence indicates that the addition of low doses of antiangiogenic drugs to ICIs substantially improves antitumor immunity. In this review, we outline the impact that the reciprocal interactions occurring between tumor angiogenesis and immune cells have on the immune evasion and clinical progression of BC. In addition, we overview preclinical and clinical studies that are presently evaluating the therapeutic effectiveness of combining ICIs with antiangiogenic drugs in BC patients.
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- 2023
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8. ZNF281/Zfp281 is a target of miR‐1 and counteracts muscle differentiation
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Sara Nicolai, Marco Pieraccioli, Artem Smirnov, Consuelo Pitolli, Lucia Anemona, Alessandro Mauriello, Eleonora Candi, Margherita Annicchiarico‐Petruzzelli, Yufang Shi, Ying Wang, Gerry Melino, and Giuseppe Raschellà
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miRs ,muscle differentiation ,soft tissue sarcomas ,Zfp281 ,ZNF281 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Defects in achieving a fully differentiated state and aberrant expression of genes and microRNAs (miRs) involved in differentiation are common to virtually all tumor types. Here, we demonstrate that the zinc finger transcription factor ZNF281/Zfp281 is down‐regulated during epithelial, muscle, and granulocytic differentiation in vitro. The expression of this gene is absent in terminally differentiated human tissues, in contrast to the elevated expression in proliferating/differentiating ones. Analysis of the 3’UTR of ZNF281/Zfp281 revealed the presence of numerous previously undescribed miR binding sites that were proved to be functional for miR‐mediated post‐transcriptional regulation. Many of these miRs are involved in differentiation pathways of distinct cell lineages. Of interest, ZNF281/Zfp281 is able to inhibit muscle differentiation promoted by miR‐1, of which ZNF281/Zfp281 is a direct target. These data suggest that down‐regulation of ZNF281/Zfp281 during differentiation in various cell types may occur through specific miRs whose expression is tissue‐restricted. In addition, we found that in rhabdomyosarcoma and leiomyosarcoma tumors, the expression of ZNF281/Zfp281 is significantly higher compared with normal counterparts. We extended our analysis to other human soft tissue sarcomas, in which the expression of ZNF281 is associated with a worse prognosis. In summary, we highlight here a new role of ZNF281/Zfp281 in counteracting muscle differentiation; its down‐regulation is at least in part mediated by miR‐1. The elevated expression of ZNF281/Zfp281 in soft tissue sarcomas warrants further analysis for its possible exploitation as a prognostic marker in this class of tumors.
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- 2020
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9. Coexistence of T-Large Granular Lymphocyte Leukemia and Peripheral T Cell Lymphoma-NOS with indolent behaviour
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Luca Guarnera, Valentina Boldrini, Gianmario Pasqualone, Carolina Cimino, Elisa Meddi, Roberta Laureana, Donata Trivigno, Giovanni Del Poeta, Alessandro Mauriello, Lucia Anemona, Massimiliano Postorino, and Maria Cantonetti
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PTCL-NOS ,LGLL ,Aggressive T-cell lymphomas ,Indolent T-cell lymphomas ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
T-cell lymphomas and leukemias are highly heterogeneous groups of rare disorders. We report a case of a 68-year-old man patient who develops two different T-cell neoplasms (Large Granular Lymphocyte Leukemia [LGLL] in 2018 and Peripheral T-cell non-Hodgkin lymphoma not otherwise specified [PTCL-NOS] in 2019) with a previous diagnosis of B-cell marginal zone lymphoma in 2010, treated with two lines of chemo-immunotherapy. The coexistence of these different T-cell neoplasms is rarely reported in literature and, moreover, is usually described as an LGLL transformation into PTCL-NOS; differently from these examples, herein the simultaneous conditions appear to be driven by different T-cell clones. Furthermore, the PTCL-NOS had a quite unusual behaviour, with a good disease control without intensive treatment. Because of these features, it could belong to a subgroup of indolent PTCL-NOS, not yet described in the WHO classification of T-cell neoplasms, which could benefit of less aggressive treatment.
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- 2022
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10. No Time to Die: How Kidney Cancer Evades Cell Death
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Carlo Ganini, Manuela Montanaro, Manuel Scimeca, Giampiero Palmieri, Lucia Anemona, Livia Concetti, Gerry Melino, Pierluigi Bove, Ivano Amelio, Eleonora Candi, and Alessandro Mauriello
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renal cell carcinoma ,cell death ,apoptosis ,ferroptosis ,pyroptosis ,necroptosis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The understanding of the pathogenesis of renal cell carcinoma led to the development of targeted therapies, which dramatically changed the overall survival rate. Nonetheless, despite innovative lines of therapy accessible to patients, the prognosis remains severe in most cases. Kidney cancer rarely shows mutations in the genes coding for proteins involved in programmed cell death, including p53. In this paper, we show that the molecular machinery responsible for different forms of cell death, such as apoptosis, ferroptosis, pyroptosis, and necroptosis, which are somehow impaired in kidney cancer to allow cancer cell growth and development, was reactivated by targeted pharmacological intervention. The aim of the present review was to summarize the modality of programmed cell death in the pathogenesis of renal cell carcinoma, showing in vitro and in vivo evidence of their potential role in controlling kidney cancer growth, and highlighting their possible therapeutic value.
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- 2022
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11. Case Report: Early Breast Cancer Recurrence Mimicking BIA-ALCL in a Patient With Multiple Breast Procedures
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Marco Materazzo, Gianluca Vanni, Marco Pellicciaro, Lucia Anemona, Francesca Servadei, Erika Giacobbi, Andrea Farinaccio, Chiara Adriana Pistolese, Tommaso Perretta, Marcello Chiocchi, Valentina Bruno, Federico Tacconi, Amir Sadri, Adriano De Majo, Camilla Di Pasquali, Rosaria Meucci, Francesca Santori, Maria Cotesta, and Oreste Claudio Buonomo
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breast implant associated-anaplastic large cell lymphoma ,breast cancer ,locoregional recurrence ,macro textured breast implants ,residual breast tissue ,case report ,Surgery ,RD1-811 - Abstract
Breast reconstruction plays a fundamental role in the therapeutic process of breast cancer treatment and breast implants represents the leading breast reconstruction strategy. Breast Implant Associated-Anaplastic Large Cell Lymphoma (BIA-ALCL), locoregional recurrence in the skin flap, and skin flap necrosis are well-known complications following mastectomy and immediate breast reconstruction (IBR). We report a case of locoregional cancer recurrence in the mastectomy flap mimicking BIA-ALCL, in a patient who underwent 6 breast procedures in four facilities across 15 years including immediate breast reconstruction with macrotextured breast implants. Despite the rate and onset of the disease, clinicians should be aware of BIA-ALCL. Due to the risk of false negative results of fine needle aspiration, clinical suspicion of BIA-ALCL should drive clinicians' choices, aside from cytological results. In the present case, surgical capsulectomy of the abnormal periprosthesic tissue revealed locoregional recurrence.
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- 2021
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12. Ultrasound-Guided Laser Ablation After Excisional Vacuum-Assisted Breast Biopsy for Small Malignant Breast Lesions: Preliminary Results
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Tommaso Perretta MD, Rosaria Meucci MD, Chiara Adriana Pistolese MD, Guglielmo Manenti PhD, Carla Di Stefano MD, Gianluca Vanni MD, Lucia Anemona PhD, Donatella Ferrari MD, Feliciana Lamacchia MD, Vincenzo De Stasio MD, and Oreste Claudio Buonomo PhD
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: The purpose of this preliminary study is to evaluate the feasibility of the excisional ultrasound (US) guided vacuum-assisted breast biopsy (VAE), followed by US-guided Laser Interstitial Thermal Therapy (LITT) in the treatment of unifocal ductal breast carcinomas ≤ 1 cm and estimate the ablation rate analyzing the final histopathological results after subsequent surgical excision. Methods: In a single session 11 female patients with unifocal less than a centimeter breast cancer underwent 2 different minimally invasive percutaneous US-guided techniques: a VAE breast biopsy with an 8 G needle to remove the lesion and, immediately after, a LITT ablation in the biopsy site. Four weeks later, all patients underwent radiological follow-up. Afterward, a systematic surgery was performed, the ablation rate was calculated, and iconographic and histological features were correlated. Results: Average maximum diameter of the lesions was 7.6 mm (5-10 mm). No patient reported pain or discomfort during procedure. 1/11 patient (9.1%) reported an early minor complication (a small superficial skin burn). After surgical excision, the histopathological evaluation reported in 10/11 cases (90.9%) complete ablation of the target lesion. In only one case (9.1%) residual cancer was detected. The necrotic-hemorrhagic cavities showed a mean maximum diameter of 27.3 mm (20-35 mm). Conclusions: Laser ablation performed after excisional biopsy could be considered a valid alternative to surgical excision for the treatment of lesions ≤ 1 cm, if carried out by expert radiologists. The association of these minimally invasive percutaneous methods has proven to be reliable, fast, and safe with an ablation rate of 90.9% and excellent aesthetic results. RM and CESM are potentially able to quantifying treatment results and to follow-up the ablation effects.
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- 2021
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13. The ETS Homologous Factor (EHF) Represents a Useful Immunohistochemical Marker for Predicting Prostate Cancer Metastasis
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Manuel Scimeca, Manuela Montanaro, Rita Bonfiglio, Lucia Anemona, Enrico Finazzi Agrò, Anastasios D. Asimakopoulos, Roberto Bei, Vittorio Manzari, Nicoletta Urbano, Erica Giacobbi, Francesca Servadei, Elena Bonanno, Orazio Schillaci, and Alessandro Mauriello
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prostate cancer ,ETS homologous factor ,metastasis ,histological marker ,Medicine (General) ,R5-920 - Abstract
The main aim of this study was to investigate the risk of prostate cancer metastasis formation associated with the expression of ETS homologous factor (EHF) in a cohort of bioptic samples. To this end, the expression of EHF was evaluated in a cohort of 152 prostate biopsies including primary prostate cancers that developed metastatic lesions, primary prostate cancers that did not develop metastasis, and benign lesions. Data here reported EHF as a candidate immunohistochemical prognostic biomarker for prostate cancer metastasis formation regardless of the Gleason scoring system. Indeed, our data clearly show that primary lesions with EHF positive cells ≥40% had a great risk of developing metastasis within five years from the first diagnosis. Patients with these lesions had about a 40-fold increased risk of developing metastasis as compared with patients with prostate lesions characterized by a percentage of EHF positive cells ≤30%. In conclusion, the immunohistochemical evaluation of EHF could significantly improve the management of prostate cancer patients by optimizing the diagnostic and therapeutic health procedures and, more important, ameliorating the patient’s quality of life.
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- 2022
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14. The role of obesity in carotid plaque instability: interaction with age, gender, and cardiovascular risk factors
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Valentina Rovella, Lucia Anemona, Marina Cardellini, Manuel Scimeca, Andrea Saggini, Giuseppe Santeusanio, Elena Bonanno, Manuela Montanaro, Iacopo Maria Legramante, Arnaldo Ippoliti, Nicola Di Daniele, Massimo Federici, and Alessandro Mauriello
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Obesity ,Stroke ,Carotid ,Histology ,Metabolic syndrome ,Age ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background In the last decade, several studies have reported an unexpected and seemingly paradoxical inverse correlation between BMI and incidence of cardiovascular diseases. This so called “obesity paradox effect” has been mainly investigated through imaging methods instead of histologic evaluation, which is still the best method to study the instability of carotid plaque. Therefore, the purpose of our study was to evaluate by histology the role of obesity in destabilization of carotid plaques and the interaction with age, gender and other major cerebrovascular risk factors. Methods A total of 390 carotid plaques from symptomatic and asymptomatic patients submitted to endarterectomy, for whom complete clinical and laboratory assessment of major cardiovascular risk factors was available, were studied by histology. Patients with a BMI ≥ 30.0 kg/m2 were considered as obese. Data were analyzed by multivariate logistic regression and for each variable in the equation the estimated odds ratio (OR) was calculated. Results Unstable carotid plaque OR for obese patients with age
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- 2018
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15. High Sensitivity C-Reactive Protein Increases the Risk of Carotid Plaque Instability in Male Dyslipidemic Patients
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Manuel Scimeca, Manuela Montanaro, Marina Cardellini, Rita Bonfiglio, Lucia Anemona, Nicoletta Urbano, Elena Bonanno, Rossella Menghini, Viviana Casagrande, Eugenio Martelli, Francesca Servadei, Erica Giacobbi, Arnaldo Ippoliti, Roberto Bei, Vittorio Manzari, Massimo Federici, Orazio Schillaci, and Alessandro Mauriello
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atherosclerosis ,hs-CRP ,carotid plaque ,cardiovascular risk factors ,dyslipidemic patients ,Medicine (General) ,R5-920 - Abstract
Background: The aim of this study was to evaluate how the high sensitivity C-reactive protein (hs-CRP) values influence the risk of carotid plaque instability in association with other cardiovascular risk factors. Methods: One hundred and fifty-six carotid plaques from both symptomatic and asymptomatic patients requiring surgical carotid endarterectomy were retrospectively collected. According to the modified American Heart Association, atherosclerosis plaques have been histologically distinguished into unstable and stable. The following anamnestic and hematochemical data were also considered: age, gender, hypertension, diabetes mellitus, smoking habit, therapy, low-density lipoprotein (LDL)-C, kidney failure and hs-CRP. Results: The results of our study clearly show that high levels of hs-CRP significantly increase the carotid plaque instability in dyslipidemic patients. Specifically, a 67% increase of the risk of carotid plaque instability was observed in patients with high LDL-C. Therefore, the highest risk was observed in male dyslipidemic patients 2333 (95% CI 0.73–7.48) and in aged female patients 2713 (95% CI 0.14–53.27). Discussion: These data strongly suggest a biological relationship between the hs-CRP values and the alteration of lipidic metabolism mostly in male patients affected by carotid atherosclerosis. The measurement of hs-CRP might be useful as a potential screening tool in the prevention of atheroscletotic disease.
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- 2021
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16. Persistence of SARS-CoV-2 Viral RNA in Nasopharyngeal Swabs after Death: An Observational Study
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Francesca Servadei, Silvestro Mauriello, Manuel Scimeca, Bartolo Caggiano, Marco Ciotti, Lucia Anemona, Manuela Montanaro, Erica Giacobbi, Michele Treglia, Sergio Bernardini, Luigi Tonino Marsella, Nicoletta Urbano, Orazio Schillaci, and Alessandro Mauriello
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SARS-CoV-2 ,pandemic ,autopsy ,medico-legal procedures ,Biology (General) ,QH301-705.5 - Abstract
The aim of this study was to investigate the persistence of SARS-CoV-2 in post-mortem swabs of subjects who died from SARS-CoV-2 infection. The presence of the virus was evaluated post-mortem from airways of 27 SARS-CoV-2 positive patients at three different time points (T1 2 h; T2 12 h; T3 24 h) by real-time PCR. Detection of antibodies to SARS-CoV-2 was performed by Maglumi 2019-nCoV IgM/IgG chemiluminescence assay. SARS-CoV-2 viral RNA was still detectable in 70.3% of cases within 2 h after death and in 66,6% of cases up to 24 h after death. Our data showed an increase of the viral load in 78,6% of positive individuals 24 h post-mortem (T3) in comparison to that evaluated 2 h after death (T1). Noteworthy, we detected a positive T3 post-mortem swab (24 h after death) from 4 subjects who were negative at T1 (2 h after death). The results of our study may have an important value in the management of deceased subjects not only with a suspected or confirmed diagnosis of SARS-CoV-2, but also for unspecified causes and in the absence of clinical documentation or medical assistance.
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- 2021
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17. The Paradox Effect of Calcification in Carotid Atherosclerosis: Microcalcification Is Correlated with Plaque Instability
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Manuela Montanaro, Manuel Scimeca, Lucia Anemona, Francesca Servadei, Erica Giacobbi, Rita Bonfiglio, Elena Bonanno, Nicoletta Urbano, Arnaldo Ippoliti, Giuseppe Santeusanio, Orazio Schillaci, and Alessandro Mauriello
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atherosclerosis ,plaque instability ,carotid plaque ,microcalcification ,macrocalcification ,macrophage polarization ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Background: this study aims to investigate the possible association among the histopathologic features of carotid plaque instability, the presence of micro- or macrocalcifications, the expression of in situ inflammatory biomarkers, and the occurrence of the major risk factors in this process in a large series of carotid plaques. Methods: a total of 687 carotid plaques from symptomatic and asymptomatic patients were collected. Histological evaluation was performed to classify the calcium deposits in micro or macrocalcifications according to their morphological features (location and size). Immunohistochemistry was performed to study the expression of the main inflammatory biomarkers. Results: results here reported demonstrated that calcifications are very frequent in carotid plaques, with a significant difference between the presence of micro- and macrocalcifications. Specifically, microcalcifications were significantly associated to high inflamed unstable plaques. Paradoxically, macrocalcifications seem to stabilize the plaque and are associated to a M2 macrophage polarization instead. Discussion: the characterization of mechanisms involved in the formation of carotid calcifications can lay the foundation for developing new strategies for the management of patients affected by carotid atherosclerosis. Data of this study could provide key elements for an exhaustive evaluation of carotid plaque calcifications allowing to establish the risk of associated clinical events.
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- 2021
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18. p63 Is a Promising Marker in the Diagnosis of Unusual Skin Cancer
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Artem Smirnov, Lucia Anemona, Flavia Novelli, Cristina M. Piro, Margherita Annicchiarico-Petruzzelli, Gerry Melino, and Eleonora Candi
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p63 ,p40 ,skin cancer ,squamous cell carcinoma ,basal cell carcinoma ,merkel cell carcinoma ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Skin cancer is the most common type of cancer worldwide. Ozone depletion and climate changes might cause a further increase in the incidence rate in the future. Although the early detection of skin cancer enables it to be treated successfully, some tumours can evolve and become more aggressive, especially in the case of melanoma. Therefore, good diagnostic and prognostic markers are needed to ensure correct detection and treatment. Transcription factor p63, a member of the p53 family of proteins, plays an essential role in the development of stratified epithelia such as skin. In this paper, we conduct a comprehensive review of p63 expression in different types of skin cancer and discuss its possible use in the diagnosis and prognosis of cutaneous tumours.
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- 2019
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19. Dysplasia of Granulocytes in a Patient with HPV Disease, Recurrent Infections, and B Lymphopenia: A Novel Variant of WHIM Syndrome?
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Viviana Moschese, Giusella M. F. Moscato, Erica Giacobbi, Lucia Anemona, Silvia Di Cesare, Gigliola Di Matteo, Massimo Andreoni, and Alessandro Mauriello
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dysplasia of granulocytes ,B lymphopenia ,HPV disease ,WHIM ,myelokathexis ,Pediatrics ,RJ1-570 - Abstract
WHIM syndrome is a condition in which affected persons have chronic peripheral neutropenia, lymphopenia, abnormal susceptibility to human papilloma virus infection, and myelokathexis. Myelokathexis refers to the retention of mature neutrophils in the bone marrow (BM), which accounts for degenerative changes and hypersegmentation. Most patients present heterozygous autosomal dominant mutations of the gene encoding CXCR4. Consequently, aberrant CXCL12/CXCR4 signaling impairs the receptor downregulation causing hyperactivation (gain-of-function) that affects BM homing for myelopoiesis and lymphopoiesis and the release of neutrophils in the bloodstream. We report the case of a 26-year-old female with severe foot and hand cutaneous warts since childhood, recalcitrant genital condylomatas, bacterial infections, and intraepithelial cervical neoplasia. Laboratory tests revealed severe B lymphopenia and HPV high and low risk types. HIV testing was negative. Not only CXCR4 but also GATA2, NEMO, and CD40L gene mutations were excluded. BM smears revealed, in the presence of a normal cellularity, hyperplasia of myeloid cells (MPO positive) and karyorrhexis, especially in neutrophils and eosinophils. Of note, neutrophils with altered lobation of nuclei connected by long thin chromatin filaments were observed. Our patient presented a clinical and histological picture reminiscent of WHIM in the presence of normal peripheral neutrophil counts and wild-type CXCR4 gene. Although the BM did not reveal a classical pattern of myelokathexis, the observation of consistent signs of neutrophil dysplasia has fuelled the hypothesis of a novel WHIM variant or a novel immunodeficiency. We speculate that abnormalities that affect CXCR4/CXCL12 pair, including GRK levels or activity, might be responsible for this WHIM-like disorder.
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- 2017
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20. Increased Sympathetic Renal Innervation in Hemodialysis Patients Is the Anatomical Substrate of Sympathetic Hyperactivity in End‐Stage Renal Disease
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Alessandro Mauriello, Valentina Rovella, Lucia Anemona, Francesca Servadei, Elena Giannini, Pierluigi Bove, Alessandro Anselmo, Gerry Melino, and Nicola Di Daniele
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end‐stage renal disease ,hemodialysis ,histology ,sympathetic renal innervation ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background Renal denervation represents an emerging treatment for resistant hypertension in patients with end‐stage renal disease, but data about the anatomic substrate of this treatment are lacking. Therefore, the aim of this study was to investigate the morphological basis of sympathetic hyperactivity in the setting of hemodialysis patients to identify an anatomical substrate that could warrant the use of this new therapeutic approach. Methods and Results The distribution of sympathetic nerves was evaluated in the adventitia of 38 renal arteries that were collected at autopsy or during surgery from 25 patients: 9 with end‐stage renal disease on dialysis (DIAL group) and 16 age‐matched control nondialysis patients (CTRL group). Patients in the DIAL group showed a significant increase in nerve density in the internal area of the peri‐adventitial tissue (within the first 0.5 mm of the beginning of the adventitia) compared with the CTRL group (4.01±0.30 versus 2.87±0.28×mm2, P=0.01). Regardless of dialysis, hypertensive patients with signs of severe arteriolar damage had a greater number of nerve endings in the most internal adventitia, and this number was significantly higher than in patients without hypertensive arteriolar damage (3.90±0.36 versus 2.87±0.41×mm2, P=0.04), showing a correlation with hypertensive arteriolar damage rather than with hypertensive clinical history. Conclusions The findings from this study provide a morphological basis underlying sympathetic hyperactivity in patients with end‐stage renal disease and might offer useful information to improve the use of renal denervation in this group of patients.
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- 2015
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21. FEASIBILITY AND SAFETY OF AWAKE ONCOPLASTIC SURGERY FOR BREAST CANCER
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Gianluca Vanni, Marco Pellicciaro, Marco Materazzo, Giada Iafrate, Jonathan Caspi, Roberto Fiorito, Ilaria Portarena, Francesca Servadei, Erica Giacobbi, Lucia Anemona, and Oreste Claudio Buonomo
- Published
- 2022
22. A thymic hyperplasia-related reversible complete atrioventricular block: When compression is more important than compressor
- Author
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Eugenio Martuscelli, Lucia Anemona, Gianluca Massaro, Giuseppe Sangiorgi, Enrica Mariano, Giuseppe Stifano, Vincenzo Ambrogi, and Gaetano Chiricolo
- Subjects
Adult ,medicine.medical_specialty ,AV block ,Vagal nerve ,medicine.medical_treatment ,Parasympathetic nervous system ,Syncope ,Settore MED/06 ,Electrocardiography ,Young Adult ,Internal medicine ,Block (telecommunications) ,medicine ,Humans ,Atrioventricular Block ,Vagus nerve compression ,ECG ,business.industry ,Vagus Nerve ,Hyperplasia ,Compression (physics) ,medicine.disease ,Thymectomy ,medicine.anatomical_structure ,Cardiology ,Thymus Hyperplasia ,Cardiology and Cardiovascular Medicine ,business ,Atrioventricular block - Abstract
A 19-year-old patient presented for syncope with third-degree AV block (TDAVB) at ECG. A chest-CT showed a thymic mass that could be responsible for TDAVB due to extrinsic vagal nerve compression. Thymectomy led to complete AV block resolution. An extrinsic vagal compression mechanism should be considered among causes of complete atrioventricular block.
- Published
- 2021
23. PD-L1 Testing In Metastatic Triple Negative Breast Cancer: Results Of An Italian Survey
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Bruna Cerbelli, Alessio Cirillo, Giulia Pomati, Angelina Pernazza, Andrea Ascione, Simona Pisegna, Annalinda Pisano, Martina Leopizzi, Maria Gemma Pignataro, Leopoldo Costarelli, Antonino Mulè, Andrea Vecchione, Piera Catalano, Luigi Coppola, Giuseppe Perrone, Letizia Perracchio, Lucia Anemona, Antonio Mastracchio, Stefano Nardi, Renato Reitano, Annalisa Massari, Lucia Rosalba Grillo, Fabrizio Liberati, Carlo Della Rocca, Paolo Marchetti, Andrea Botticelli, and Giulia D’Amati
- Abstract
Background: Immunotherapy has revolutionized the approach to metastatic triple-negative breast cancers (mTNBC). Atezolizumab was approved for patients with mTNBC whose tumours express PD-L1, determined by SP 142 assay. To assess the availability and practice of SP142 test we administered a survey to all the 15 Pathology Departments of the Lazio Region during a 6-months period.Methods: The survey comprised 12 questions regarding the availability of SP142 in the Pathology Departments, the percentage of positive tests, the difficulties of pathologists in case close to cut-off value and the tested samples.Results: The SP142 assay was available only in 8 Centers. In case of positive result, most Centers (5/8, 62.5%) reported values of PD-L1 expression ranging from > 1 to Conclusion: Our results raise some important issues concerning the evaluation of PD-L1 in the “real-life” setting, providing strategies for its implementation.
- Published
- 2022
24. Macrophage Activation and M2 Polarization in Wound Bed of Diabetic Patients Treated by Dermal/Epidermal Substitute Nevelia
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Marco Meloni, Alessandro Mauriello, Francesca Servadei, Manuela Montanaro, Manuel Scimeca, Laura Giurato, Artem Smirnov, Eleonora Candi, Valentina Izzo, Luigi Uccioli, and Lucia Anemona
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Wound Healing ,0303 health sciences ,business.industry ,Macrophages ,Macrophage polarization ,General Medicine ,M2 polarization ,Macrophage Activation ,Pharmacology ,medicine.disease ,Diabetic foot ,Diabetic Foot ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Diabetes Mellitus ,Humans ,Medicine ,Macrophage ,Surgery ,business ,Wound healing ,030304 developmental biology - Abstract
Clinical evidences have shown good results using dermal/epidermal substitutes (DESs) to treat diabetic foot ulcers. Recent studies suggest that, in addition to their scaffold action, DESs may favor wound healing by influencing wound bed inflammatory cells. This study aims to investigate whether DES may influence the inflammatory infiltrate and macrophages polarization toward a reparative phenotype. Fifteen diabetic patients with chronic foot ulcers have been randomly enrolled: 5 treated only by standard of care, served as control group (CG), and 10 treated with DES composed of type 1 bovin collagen (Nevelia, SYMATESE) considered as test group (TG). A biopsy was taken at baseline (T0) and after 30 days (T1). From bioptic paraffin specimen histological, immunohistochemical, and immunofluorescence analysis was performed. Immunohistochemistry reactions evaluated the number of M1 macrophage (CD38+) and M2 macrophage (CD163+). TG patients displayed general macrophage activation and their greater polarization toward M2 subpopulation 30 days after DES implant, compared with CG. From T0 to T1 there was a significant decrease of CD38+ (230 ± 42 and 135 ± 48 mm2, respectively; P < .001) and significant increase of CD163+ (102 ± 21 positive cells/mm2 and 366 ± 42 positive cells/mm2, respectively; P < .001). Confocal microscopy confirmed an increase of M2 cells as expressed by the reduced CD68+/CD163+ ratio. After 6 months of observation 6 patients (60%) of the TG completely healed, while only 1 patient (20%) healed in the CG ( P < .01). The tested DES makes possible to treat diabetic foot ulcers inducing tissue reparative processes through macrophage activation and M2 reparative polarization.
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- 2020
25. Panniculitis-Like Presentation of Extracavitary Primary Effusion Lymphoma
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Monia Di Prete, Lucia Anemona, Simone Facchetti, Vito Mario Rapisarda, and Andrea Saggini
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Panniculitis ,Skin Neoplasms ,HIV Infections ,Dermatology ,Pathology and Forensic Medicine ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Immunophenotyping ,immune system diseases ,Antiretroviral Therapy, Highly Active ,Lymphoma, Primary Effusion ,hemic and lymphatic diseases ,medicine ,Humans ,integumentary system ,business.industry ,virus diseases ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,Lymphoma ,Serous fluid ,Effusion ,Primary effusion lymphoma ,Presentation (obstetrics) ,Differential diagnosis ,business - Abstract
Primary effusion lymphoma (PEL) is defined as a HHV-8-associated large B-cell lymphoma, which favors HIV-infected young adults, typically presenting as a serous (pleural, pericardial, or peritoneal) effusion with no identifiable tumor mass. Uncommon instances of lymphoid proliferations with the same morphology, immunophenotype, and molecular features as PEL, but occurring as a solid tumor mass without serous cavities involvement, have been termed extracavitary (or solid) variant of PEL. We hereby report the exceptional case of a HIV-associated extracavitary PEL primarily localized to the skin and exhibiting a panniculitis-like presentation. Primary cutaneous presentation of extracavitary PEL is exceedingly uncommon, with only 6 cases previously described in the literature. In light of its atypical immunophenotype, the differential diagnosis in case of skin involvement by extracavitary PEL is challenging: demonstration of HHV-8 infection in neoplastic cells is of pivotal importance. Our case is further atypical in that the lymphoid proliferation underwent complete and protracted regression solely by establishment of highly active antiretroviral therapy.
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- 2020
26. A Prospective Evaluation of Tru-Cut Biopsy and Fine-needle Aspiration Cytology in Male Breast Cancer Detection
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Feliciana Lamacchia, Giulia Claroni, Francesca Servadei, Chiara Adriana Pistolese, Alberto Collura, Marco Pellicciaro, Lucia Anemona, Marco Materazzo, Michela Censi, Gianluca Vanni, and Tommaso Perretta
- Subjects
Male ,Cancer Research ,medicine.medical_specialty ,Cytodiagnosis ,Biopsy, Fine-Needle ,Breast Neoplasms ,Malignancy ,Sensitivity and Specificity ,General Biochemistry, Genetics and Molecular Biology ,Prospective evaluation ,Breast Neoplasms, Male ,03 medical and health sciences ,0302 clinical medicine ,Fine needle aspiration cytology ,Biopsy ,medicine ,Humans ,Prospective Studies ,skin and connective tissue diseases ,Trucut biopsy ,Pharmacology ,medicine.diagnostic_test ,business.industry ,Carcinoma ,Ductal carcinoma ,medicine.disease ,Aspiration cytology ,body regions ,030220 oncology & carcinogenesis ,Male breast cancer ,Radiology ,business ,Research Article - Abstract
Background: Male breast-cancer (MBC) is often diagnosed late. Our purpose was to evaluate fine-needle aspiration cytology (FNAC) versus Tru-Cut biopsy (TCNB) in MBC diagnosis. Patients and Methods: Men with suspicious breast lesions were prospectively enrolled; 54 met the inclusion criteria and underwent FNAC and TCNB. FNAC, TCNB and gold-standard results were compared. Results: Unsatisfactory results were 11.1% after FNAC and none after TCNB (p=0.027). After gold-standard evaluation, the diagnosis of FNAC and TCNB was confirmed, respectively, in 63.0% and 98.1% and changed in 37.0% and 1.9% (p
- Published
- 2020
27. Does Age Matter? Estimating Risks of Locoregional Recurrence After Breast-conservative Surgery
- Author
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Rosaria Meucci, Agostino Chiaravalloti, Marco Materazzo, Paolo Orsaria, Gianluca Vanni, Ljuba Morando, Jonathan Caspi, Marco Pellicciaro, Lucia Anemona, Rosaria Barbarino, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Ilaria Portarena, Camilla Deiana, Maria Rolando D'Angelillo, and Tommaso Perretta
- Subjects
Risk ,Cancer Research ,medicine.medical_specialty ,Breast Neoplasms ,Subgroup analysis ,Mastectomy, Segmental ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Biomarkers, Tumor ,medicine ,Humans ,Operation time ,In patient ,Aged ,Retrospective Studies ,Aged, 80 and over ,Postoperative Care ,Pharmacology ,business.industry ,Incidence (epidemiology) ,Middle Aged ,Prognosis ,Tailored treatment ,medicine.disease ,Surgery ,Breast conservative surgery ,Receptors, Estrogen ,Case-Control Studies ,030220 oncology & carcinogenesis ,Ki67 index ,Female ,Neoplasm Recurrence, Local ,business ,Research Article - Abstract
Background/Aim: In 2016, in the United States, more than 50% of breast cancer (BC) cases were diagnosed in patients older than 60 years of age. Our study aimed to estimate the risk of locoregional recurrence (LR) in patients who underwent breast-conservative treatment (BCT), according to age. Patients and Methods: This retrospective monocentric study analyzed 613 cases of patients who underwent BCT between 2003 and 2014. Patients were divided into groups according to age: Under70 (under 70 years old) and Over70 (above 70 years old). Margins width, histology results, prognostic and predictive factors were compared. Subgroup analysis was performed for patients who experienced LR. Results: LR Incidence among Under70 and Over70 was 5.4% and 1.7%, respectively (p
- Published
- 2020
28. High Sensitivity C-Reactive Protein Increases the Risk of Carotid Plaque Instability in Male Dyslipidemic Patients
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Francesca Servadei, Marina Cardellini, Rita Bonfiglio, Manuela Montanaro, Roberto Bei, Viviana Casagrande, Arnaldo Ippoliti, Alessandro Mauriello, Vittorio Manzari, Erica Giacobbi, Lucia Anemona, Rossella Menghini, Orazio Schillaci, Eugenio Martelli, Nicoletta Urbano, Massimo Federici, Manuel Scimeca, and Elena Bonanno
- Subjects
cardiovascular risk factors ,Medicine (General) ,medicine.medical_specialty ,Plaque instability ,carotid plaque ,medicine.medical_treatment ,Clinical Biochemistry ,Settore MED/08 ,Carotid endarterectomy ,Disease ,dyslipidemic patients ,Settore MED/04 ,Asymptomatic ,Article ,R5-920 ,Internal medicine ,Diabetes mellitus ,Medicine ,Kidney ,biology ,business.industry ,C-reactive protein ,medicine.disease ,hs-CRP ,Settore MED/46 ,medicine.anatomical_structure ,Cardiology ,biology.protein ,medicine.symptom ,atherosclerosis ,business ,Lipoprotein - Abstract
Background: The aim of this study was to evaluate how the high sensitivity C-reactive protein (hs-CRP) values influence the risk of carotid plaque instability in association with other cardiovascular risk factors. Methods: One hundred and fifty-six carotid plaques from both symptomatic and asymptomatic patients requiring surgical carotid endarterectomy were retrospectively collected. According to the modified American Heart Association, atherosclerosis plaques have been histologically distinguished into unstable and stable. The following anamnestic and hematochemical data were also considered: age, gender, hypertension, diabetes mellitus, smoking habit, therapy, low-density lipoprotein (LDL)-C, kidney failure and hs-CRP. Results: The results of our study clearly show that high levels of hs-CRP significantly increase the carotid plaque instability in dyslipidemic patients. Specifically, a 67% increase of the risk of carotid plaque instability was observed in patients with high LDL-C. Therefore, the highest risk was observed in male dyslipidemic patients 2333 (95% CI 0.73–7.48) and in aged female patients 2713 (95% CI 0.14–53.27). Discussion: These data strongly suggest a biological relationship between the hs-CRP values and the alteration of lipidic metabolism mostly in male patients affected by carotid atherosclerosis. The measurement of hs-CRP might be useful as a potential screening tool in the prevention of atheroscletotic disease.
- Published
- 2021
29. The risk of carotid plaque instability in patients with metabolic syndrome is higher in women with hypertriglyceridemia
- Author
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Massimo Federici, Manuel Scimeca, Alessandro Mauriello, Manuela Montanaro, Rita Bonfiglio, Annalisa Noce, Arnaldo Ippoliti, Valentina Rovella, Nicola Di Daniele, Erica Giacobbi, Marina Cardellini, Francesca Di Daniele, P. Borboni, Lucia Anemona, Francesca Servadei, and Iacopo Maria Legramante
- Subjects
Carotid Artery Diseases ,Male ,Time Factors ,Endocrinology, Diabetes and Metabolism ,Settore MED/09 ,030204 cardiovascular system & hematology ,0302 clinical medicine ,Risk Factors ,Original Investigation ,Aged, 80 and over ,Hypertriglyceridemia ,Incidence ,Incidence (epidemiology) ,Middle Aged ,Prognosis ,Metabolic syndrome ,Plaque, Atherosclerotic ,Italy ,Disease Progression ,Cardiology ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,medicine.medical_specialty ,Histology ,Risk Assessment ,Asymptomatic ,03 medical and health sciences ,Sex Factors ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Diseases of the circulatory (Cardiovascular) system ,In patient ,Triglycerides ,Aged ,Retrospective Studies ,Carotid ,Angiology ,Rupture, Spontaneous ,business.industry ,Odds ratio ,medicine.disease ,RC666-701 ,Post‐menopause ,business ,Biomarkers ,030217 neurology & neurosurgery - Abstract
Background Metabolic syndrome certainly favors growth of carotid plaque; however, it is uncertain if it determines plaque destabilization. Furthermore, it is likely that only some components of metabolic syndrome are associated with increased risk of plaque destabilization. Therefore, we evaluated the effect of different elements of metabolic syndrome, individually and in association, on carotid plaques destabilization. Methods A total of 186 carotid endarterectomies from symptomatic and asymptomatic patients were histologically analysed and correlated with major cardiovascular risk factors. Results Metabolic syndrome, regardless of the cluster of its components, is not associated with a significant increase in risk of plaque destabilization, rather with the presence of stable plaques. The incidence of unstable plaques in patients with metabolic syndrome is quite low (43.9 %), when compared with that seen in the presence of some risk factors, but significantly increases in the subgroup of female patients with hypertriglyceridemia, showing an odds ratio of 3.01 (95% CI, 0.25–36.30). Conclusions Our data may help to identify patients with real increased risk of acute cerebrovascular diseases thus supporting the hypothesis that the control of hypertriglyceridemia should be a key point on prevention of carotid atherosclerotic plaque destabilization, especially in post-menopausal female patients.
- Published
- 2021
30. Oral manifestation of indolent T‐cell lymphoproliferative disorder of the gastrointestinal tract: A potential diagnostic pitfall
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Francesca Baciorri, Monia Di Prete, Anna Giulia Zizzari, Lucia Anemona, and Andrea Saggini
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Male ,Pathology ,medicine.medical_specialty ,Gastrointestinal tract ,Histology ,Gastrointestinal Diseases ,business.industry ,Biopsy ,T-Lymphocytes ,T cell ,Remission Induction ,Aftercare ,Dermatology ,CD8-Positive T-Lymphocytes ,medicine.disease ,Lymphoproliferative Disorders ,Peripheral T-cell lymphoma ,Pathology and Forensic Medicine ,medicine.anatomical_structure ,Tongue ,Adrenal Cortex Hormones ,medicine ,Humans ,business ,Aged - Published
- 2020
31. Timp3 deficiency affects the progression of DEN-related hepatocellular carcinoma during diet-induced obesity in mice
- Author
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Viviana Casagrande, Mara D'Onofrio, Ivan Arisi, Giulia Iuliani, Alessandro Mauriello, Maria Mavilio, Lorenzo De Angelis, Lucia Anemona, Massimo Federici, and Rossella Menghini
- Subjects
Male ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,TIMP3 ,Settore MED/09 - Medicina Interna ,Hepatocellular carcinoma ,Endocrinology, Diabetes and Metabolism ,Settore MED/08 ,030209 endocrinology & metabolism ,Inflammation ,Endogeny ,030204 cardiovascular system & hematology ,Diet, High-Fat ,Obesity ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Mediator ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Animals ,Diethylnitrosamine ,Mice, Knockout ,Tissue Inhibitor of Metalloproteinase-3 ,2. Zero hunger ,business.industry ,Forkhead Box Protein M1 ,Liver Neoplasms ,Cancer ,General Medicine ,Tissue inhibitor of metalloproteinase ,medicine.disease ,3. Good health ,Fatty Liver ,Mice, Inbred C57BL ,Disease Progression ,FOXM1 ,medicine.symptom ,business ,Signal Transduction - Abstract
Obesity and low-grade inflammation are associated with an increased risk of hepatocellular carcinoma (HCC), a leading cause of cancer-related death worldwide. The tissue inhibitor of metalloproteinase (TIMP) 3, an endogenous inhibitor of protease activity that represents a key mediator of inflammation, is reduced in inflammatory metabolic disorders and cancer. In contrast, Timp3-deficient mice (Timp3−/−) are highly resistant to developing HCC in response to a diethylnitrosamine (DEN); therefore, we aimed to elucidate the biological role of genetic loss of Timp3 in obesity-related hepatocarcinogenesis. Fourteen-day-old male wild-type (wt) and Timp3−/− mice were injected with 25 mg/kg DEN or an equal volume of saline. After 4 weeks, mice were randomized into two dietary groups and fed either normal or high-fat diet and allowed to grow until 32 weeks of age. Liver histological features were analyzed, and differentially expressed genes in the liver were quantified. In Timp3−/− mice fed with the obesogenic diet, despite the increase in liver steatosis and inflammation, both the number of tumors and the total tumor size are significantly reduced 30 weeks post-DEN injection, compared to control mice. Moreover, Timp3 deletion in hepatocarcinogenesis during obesity is associated with a reduction in FoxM1 transcriptional activity through H19/miR-675/p53 pathway. This study suggests that Timp3 ablation leads to cell cycle perturbation, at least in part by repressing FoxM1 transcriptional activity through H19/miR-675/p53 pathway.
- Published
- 2019
32. Persistence of SARS-CoV-2 Viral RNA in Nasopharyngeal Swabs after Death: An Observational Study
- Author
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Orazio Schillaci, Manuela Montanaro, Manuel Scimeca, Lucia Anemona, Erica Giacobbi, Sergio Bernardini, Francesca Servadei, Luigi Tonino Marsella, Nicoletta Urbano, Marco Ciotti, Bartolo Caggiano, Michele Treglia, Alessandro Mauriello, and Silvestro Mauriello
- Subjects
0301 basic medicine ,Microbiology (medical) ,viruses ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Settore MED/08 ,Autopsy ,Microbiology ,Virus ,Persistence (computer science) ,03 medical and health sciences ,0302 clinical medicine ,autopsy ,Virology ,Medicine ,Viral rna ,030216 legal & forensic medicine ,lcsh:QH301-705.5 ,medico-legal procedures ,biology ,SARS-CoV-2 ,business.industry ,allergology ,Communication ,pandemic ,030104 developmental biology ,lcsh:Biology (General) ,biology.protein ,Observational study ,Antibody ,business ,Viral load - Abstract
Background: The aim of this study was to investigate the persistence of SARS-CoV-2 in post-mortem swabs of subjects who died from SARS-CoV-2 infection. Methods: The presence of the virus was evaluated post-mortem from airways of 27 SARS-CoV-2 positive patients at three different time points (T1 2 hours; T2 12 hours – T3 24 hours) by real-time PCR. Detection of antibodies to SARS-CoV-2 was performed by Maglumi 2019-nCoV IgM/IgG chemiluminescence assay. Results: SARS-CoV-2 viral RNA was still detectable in 70,3% of cases within 2 hours after death and in 66,6% of cases up to 24 hours after death. Our data showed an increase of the viral load in 78,6% of positive individuals 24 hours post-mortem (T3) in comparison to that evaluated 2 hours after death (T1). Noteworthy, we detected a positive T3 post-mortem swab (24 hours after death) from 4 subjects who were negative at T1 (2 hours after death). Conclusion: The results of our study may have an important value in the management of deceased subjects not only with a suspected or confirmed diagnosis of SARS-CoV-2, but also for unspecified causes and in the absence of clinical documentation or medical assistance.
- Published
- 2021
33. New immunological potential markers for triple negative breast cancer: IL18R1, CD53, TRIM, Jaw1, LTB, PTPRCAP
- Author
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Paolo Marchetti, Gerry Melino, Alexey Antonov, Andrea Botticelli, M. Valeria Catani, Manuela Montanaro, Lucia Anemona, Chaitania Vangapandou, Alessandro Mauriello, Marchetti, Paolo [0000-0001-5170-9579], Anemona, Lucia [0000-0002-3711-2714], Botticelli, Andrea [0000-0002-6425-9893], Mauriello, Alessandro [0000-0002-7351-5676], Melino, Gerry [0000-0001-9428-5972], Catani, M. Valeria [0000-0002-7088-9242], Apollo - University of Cambridge Repository, and Catani, M Valeria [0000-0002-7088-9242]
- Subjects
0301 basic medicine ,Cancer Research ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Cancer immunity ,Jaw1 ,Prognostic markers ,03 medical and health sciences ,TRIM ,0302 clinical medicine ,Endocrinology ,Immune system ,Breast cancer ,cancer immunity ,cd53 ,il18r1 ,jaw1 ,ltb ,precision oncology ,prognostic markers ,ptprcap ,trim ,triple negative breast cancer ,Surgical oncology ,medicine ,Triple negative breast cancer ,Settore BIO/10 ,IL18R1 ,Triple-negative breast cancer ,Endocrine and Autonomic Systems ,Genetic heterogeneity ,business.industry ,Research ,Precision oncology ,PTPRCAP ,medicine.disease ,Molecular medicine ,Radiation therapy ,030104 developmental biology ,Oncology ,LTB ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,CD53 ,business - Abstract
Breast cancer (BC) is the second leading cause of cancer death in women worldwide, and settings of specific prognostic factors and efficacious therapies are made difficult by phenotypic heterogeneity of BC subtypes. Therefore, there is a current urgent need to define novel predictive genetic predictors that may be useful for stratifying patients with distinct prognostic outcomes. Here, we looked for novel molecular signatures for triple negative breast cancers (TNBCs). By a bioinformatic approach, we identified a panel of genes, whose expression was positively correlated with disease-free survival in TNBC patients, namely IL18R1, CD53, TRIM, Jaw1, LTB, and PTPRCAP, showing specific immune expression profiles linked to survival prediction; most of these genes are indeed expressed in immune cells and are required for productive lymphocyte activation. According to our hypothesis, these genes were not, or poorly, expressed in different TNBC cell lines, derived from either primary breast tumours or metastatic pleural effusions. This conclusion was further supported in vivo, as immuno-histochemical analysis on biopsies of TNBC invasive ductal carcinomas highlighted differential expression of these six genes in cancer cells, as well as in intra- and peri-tumoral infiltrating lymphocytes. Our data open to the possibility that inter-tumour heterogeneity of immune markers might have predictive value; further investigations are recommended in order to establish the real power of cancer-related immune profiles as prognostic factors. Supplementary Information The online version contains supplementary material available at 10.1007/s12672-021-00401-0.
- Published
- 2021
34. Case Report: Early Breast Cancer Recurrence Mimicking BIA-ALCL in a Patient With Multiple Breast Procedures
- Author
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Rosaria Meucci, Andrea Farinaccio, Gianluca Vanni, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Francesca Santori, Amir Sadri, Valentina Bruno, Marcello Chiocchi, Francesca Servadei, Federico Tacconi, Marco Pellicciaro, Lucia Anemona, Camilla Di Pasquali, Marco Materazzo, Erika Giacobbi, Tommaso Perretta, Adriano De Majo, and Maria Cotesta
- Subjects
medicine.medical_specialty ,locoregional recurrence ,medicine.medical_treatment ,lcsh:Surgery ,Breast procedures ,residual breast tissue ,Disease ,030230 surgery ,breast seroma ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,breast cancer ,law ,hemic and lymphatic diseases ,medicine ,case report ,skin and connective tissue diseases ,medicine.diagnostic_test ,business.industry ,Large-cell lymphoma ,lcsh:RD1-811 ,immediate breast reconstruction ,medicine.disease ,Fine-needle aspiration ,macro textured breast implants ,breast implant associated-anaplastic large cell lymphoma ,030220 oncology & carcinogenesis ,Breast implant ,Surgery ,Radiology ,Breast reconstruction ,business ,Mastectomy - Abstract
Breast reconstruction plays a fundamental role in the therapeutic process of breast cancer treatment and breast implants represents the leading breast reconstruction strategy. Breast Implant Associated-Anaplastic Large Cell Lymphoma (BIA-ALCL), locoregional recurrence in the skin flap, and skin flap necrosis are well-known complications following mastectomy and immediate breast reconstruction (IBR). We report a case of locoregional cancer recurrence in the mastectomy flap mimicking BIA-ALCL, in a patient who underwent 6 breast procedures in four facilities across 15 years including immediate breast reconstruction with macrotextured breast implants. Despite the rate and onset of the disease, clinicians should be aware of BIA-ALCL. Due to the risk of false negative results of fine needle aspiration, clinical suspicion of BIA-ALCL should drive clinicians' choices, aside from cytological results. In the present case, surgical capsulectomy of the abnormal periprosthesic tissue revealed locoregional recurrence.
- Published
- 2021
35. The Risk of Carotid Plaque Instability in Patients with Metabolic Syndrome: It is Higher in Women with Hypertriglyceridemia
- Author
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Francesca Servadei, Lucia Anemona, Marina Cardellini, Manuel Scimeca, Manuela Montanaro, Valentina Rovella, Francesca Di Daniele, Erica Giacobbi, Jacopo Maria Legramante, Annalisa Noce, Rita Bonfiglio, Patrizia Borboni, Nicola Di Daniele, Arnaldo Ippoliti, Massimo Federici, and Alessandro Mauriello
- Abstract
Background: Metabolic syndrome certainly favors growth of carotid plaque; however, it is uncertain if it determines plaque destabilization. Furthermore, it is likely that only some components of metabolic syndrome are associated with increased risk of plaque destabilization. Therefore, we evaluated the effect of different elements of metabolic syndrome, individually and in association, on carotid plaques destabilization. Methods: A total of 186 carotid endarterectomies from symptomatic and asymptomatic patients were histologically analysed and correlated with major cardiovascular risk factors. Results: Metabolic syndrome, regardless of the cluster of its components, is not associated with a significant increase in risk of plaque destabilization, rather with the presence of stable plaques. The incidence of unstable plaques in patients with metabolic syndrome is quite low (43.9%), when compared with that seen in the presence of some risk factors, but significantly increases in the subgroup of female patients with hypertriglyceridemia, showing an odds ratio of 3.01 (CI 95% 0.25 - 36.30). Conclusions: Our data may help to identify patients with real increased risk of acute cerebrovascular diseases and may support the hypothesis that the control of hypertriglyceridemia should be a key point on prevention of carotid atherosclerotic plaque destabilization, especially in post-menopausal female patients. Keywords: Metabolic syndrome, carotid, histology, hypertriglyceridemia , post-menopause.
- Published
- 2021
36. Unilateral Maculopapular Eruption in a Child: Challenge
- Author
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Andrea Saggini, Maria Vittoria Cannizzaro, Luca Bianchi, Lucia Anemona, and Augusto Orlandi
- Subjects
medicine.medical_specialty ,Text mining ,Settore MED/35 ,business.industry ,medicine ,Dermatology ,General Medicine ,business ,Pathology and Forensic Medicine - Published
- 2021
37. Persistence of SARS-CoV-2 viral RNA in nasopharyngeal swabs after death
- Author
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Manuel Scimeca, Michele Treglia, Silvestro Mauriello, Orazio Schillaci, Bartolo Caggiano, Alessandro Mauriello, Manuela Montanaro, Erica Giacobbi, Lucia Anemona, Luigi Tonino Marsella, Sergio Bernardini, Marco Ciotti, and Francesca Servadei
- Subjects
Microbiology (medical) ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,SARS-CoV-2 ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,RNA ,COVID-19 ,Viral Load ,Virology ,Persistence (computer science) ,Infectious Diseases ,Viral genetics ,Nasopharynx ,Quarantine ,Settore MED/43 ,Medicine ,Humans ,RNA, Viral ,Viral rna ,business ,Letter to the Editor - Published
- 2021
38. Juan Rosai as master of our comprehensive understanding of thymus and thymoma
- Author
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Hans Konrad Müller-Hermelink, Alexander Marx, Mirella Marino, Lucia Anemona, Libero Lauriola, and Philipp Ströbel
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Thymoma ,Thymic Tumors ,Settore MED/08 ,Review ,Diagnostic tools ,World health ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,thymus ,Disease patterns ,medicine ,Humans ,Neoplasms, Glandular and Epithelial ,Thymic carcinoma ,030304 developmental biology ,0303 health sciences ,business.industry ,Mediastinum ,Thymus Neoplasms ,medicine.disease ,Immunohistochemistry ,3. Good health ,medicine.anatomical_structure ,classification ,030220 oncology & carcinogenesis ,pathology ,business ,Mediastinal Diseases - Abstract
Summary In this study, the authors report on the activity of Juan Rosai, one of the pathologists most engaged in the definition of cells, diseases and tumors occurring in the thymus and in the mediastinum during the last 60 years. With his morphological skills and tireless interest in clarification of disease patterns, he contributed extraordinarily to expand our knowledge of the mediastinal diseases and to improve our diagnostic approach. He determined extraordinary advances also in trasmission electron microscopy and in immunohistochemistry as powerful diagnostic tools. Moreover, he proposed and promoted, together with an international panel of Pathologists, the World Health Classification of Thymic tumors as a definite progress in our comprehension and diagnostics of thymic epithelial tumors (TET). Our purpose is to review J. Rosai’s achievements in thymic normal structure, in TET and particularly in the entity now definied as “thymoma”, in distinction from the thymic carcinoma. To do this, our narrative will also be based on personal memories, longstanding collaborations and/or friendship with J. Rosai.
- Published
- 2021
39. Breast Trauma and Triple-Negative Hemorrhagic Cystic Carcinoma: Management and Treatment
- Author
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Maria Cotesta, Erica Giacobbi, Alessandra Vittoria Granai, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Francesca Servadei, Susanna Finocchiaro, Marco Pellicciaro, Lucia Anemona, Francesca Santori, Adriano De Majo, Marco Materazzo, and Gianluca Vanni
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Breast Neoplasms ,Triple Negative Breast Neoplasms ,030204 cardiovascular system & hematology ,Modified Radical Mastectomy ,Breast cysts ,03 medical and health sciences ,0302 clinical medicine ,Breast Cyst ,Biopsy ,Carcinoma ,Humans ,Medicine ,Cyst ,Mastectomy ,medicine.diagnostic_test ,Cysts ,business.industry ,Cancer ,Articles ,General Medicine ,Middle Aged ,medicine.disease ,030220 oncology & carcinogenesis ,Female ,Histopathology ,Radiology ,Neoplasm Recurrence, Local ,business - Abstract
Patient: Female, 59-year-old Final Diagnosis: Invasive breast carcinomas of no special type (NST) Symptoms: Recurrent hemorrhagic cystic lesion Medication: — Clinical Procedure: — Specialty: Surgery Objective: Challenging differential diagnosis Background: Breast trauma can always have diagnostic pitfalls. In the presence of a recurrent hemorrhagic cysts, cancer should always be suspected. Case Report: A 59-year-old woman noted a palpable mass after breast trauma from falling at home. Radiological exams showed a breast cyst with well-defined margins, with corpuscular and dense fluid components. First, a conservative approach was implemented. One week later, a fine-needle aspiration cytology (FNAC) of the mass showed bloody fluid without atypical cells. Three weeks later, the patient was emergently evaluated due to increased size of the lesion and anemia. To avoid further blood loss and due to suspected malignancy, an urgent surgical excision biopsy was planned. Histopathology revealed a poorly-differentiated carcinoma and the patient was treated with left modified radical mastectomy with axillary dissection. Adjuvant chemotherapy was administered. At 6-month follow-up, the patient was free from recurrences. Conclusions: Recurrent hemorrhagic cysts should always be investigated and considered as a possible cancer lesion. Sonography and cytological exam are the first steps in case of suspicious cysts, but false-negative results are common. In such cases, resection of the cyst should be considered. Immediate resection is valid in cases of diagnostic uncertainty or inability to assess the cyst with imaging or biopsy.
- Published
- 2020
40. Effects of Risk Factors on In Situ Expression of Proinflammatory Markers Correlated to Carotid Plaque Instability
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Manuela Montanaro, Nicola Toschi, Francesca Servadei, Arnaldo Ippoliti, Lucia Anemona, Alessandro Mauriello, Elena Bonanno, Manuel Scimeca, Giuseppe Santeusanio, and Erica Giacobbi
- Subjects
Carotid Artery Diseases ,Male ,Pathology ,medicine.medical_specialty ,Histology ,Asymptomatic ,Pathology and Forensic Medicine ,Proinflammatory cytokine ,Sex Factors ,Antigens, CD ,Risk Factors ,medicine ,Humans ,Stroke ,Aged ,Aged, 80 and over ,Tissue microarray ,business.industry ,Interleukin ,Middle Aged ,medicine.disease ,Plaque, Atherosclerotic ,Settore MED/22 ,Medical Laboratory Technology ,Carotid Arteries ,Gene Expression Regulation ,Immunohistochemistry ,Cytokines ,Female ,medicine.symptom ,business ,CD163 ,CD8 ,Biomarkers - Abstract
BACKGROUND AND AIMS Several studies demonstrated a role of active chronic inflammatory infiltrate in carotid plaques progression suggesting a possible link between cardiovascular risk factors and inflammation-related plaque instability. The aim of this study is therefore to evaluate the possible effects of cardiovascular risk factors on in situ expression of proinflammatory markers associated with carotid plaque instability. METHODS AND RESULTS A tissue microarray containing carotid plaques from 36 symptomatic (major stroke or transient ischemic attack) and 37 asymptomatic patients was built. Serial sections were employed to evaluate the expression of some inflammatory markers by immunohistochemistry [CD3, CD4a, CD8, CD20, CD86, CD163, interleukin (IL)-2, IL-6, IL-17]. Immunohistochemical data were analyzed to study the possible associations between in situ expression of inflammatory biomarker and the main cardiovascular risk factors. Our data demonstrated that plaque instability is associated with the high in situ expression of some cytokines, such as IL-2, IL-6, IL-17. Besides the female sex, none of the risk factors analyzed showed a significant association between the in situ expression of these markers and unstable plaques. A significant increase of IL-6-positive and IL-17-positive cells was observed in unstable atheromatous plaques of female patients, as compared with unstable plaques of male patients. CONCLUSIONS Plaque destabilization is certainly correlated with the presence of the major cardiovascular risk factors, however, our results showed that, with the exception of sex, their action in the evolutive process of plaque instability seems rather nonspecific, favoring a general release of proinflammatory cytokines.
- Published
- 2020
41. Colonic Adenoma with Chronic Lymphocytic Leukemia Infiltration
- Author
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B. Neri, M. Giannelli, Federico Meconi, Lucia Anemona, and Marco Pocci
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Pathology ,medicine.medical_specialty ,Adenoma ,business.industry ,Chronic lymphocytic leukemia ,Colonic Adenoma ,Gastroenterology ,Medicine ,business ,medicine.disease ,Infiltration (medical) - Published
- 2020
42. Evaluation of Concordance Between Histopathological, Radiological and Biomolecular Variables in Breast Cancer Neoadjuvant Treatment
- Author
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Ilaria Portarena, Rosaria Meucci, Ljuba Morando, Camilla Deiana, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Lucia Anemona, Andrea Grasso, Gianluca Vanni, and Marco Materazzo
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,Concordance ,Breast Neoplasms ,Disease-Free Survival ,Immunophenotyping ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Settore MED/36 ,Neoadjuvant treatment ,Internal medicine ,Receptors ,medicine ,Humans ,complete pathological response ,skin and connective tissue diseases ,Progesterone ,Histological examination ,Chemotherapy ,business.industry ,histological examination ,General Medicine ,Gold standard (test) ,Middle Aged ,medicine.disease ,MRI accuracy ,Estrogen ,Neoadjuvant Therapy ,Ki-67 Antigen ,Receptors, Estrogen ,neoadjuvant chemotherapy ,Female ,Receptors, Progesterone ,030220 oncology & carcinogenesis ,Radiological weapon ,Immunohistochemistry ,business - Abstract
Background/aim Neoadjuvant chemotherapy (NAC) for breast cancer (BC) is the gold standard treatment for locally advanced tumors (LABC) that aims at achieving a complete pathological response (pCR). Studies have been conducted to evaluate and identify te concordance between radiological, histopathological and biological variables of BC and final response to therapy, verified by definitive histological examination after surgery. Patients and methods Ninety-five BC patients were examined and subjected to NAC. Immunohistochemical markers including oestrogen-receptor (ER), progesterone-receptor (PR), Ki67 index, and human epidermal growth factor receptor 2 (HER2) score were examined before and after neoadjuvant treatment. Results Younger age and a significant decrease in ER expression were associated with better prognosis. Triple Negative (TN) and Her2-type breast cancers benefited most from neoadjuvant chemotherapy with higher frequency of pCR. Conclusion HER2-type and TN BC are correlated with best response to NAC. A statistically significant correlation between radiological images and definitive histological examination was not observed.
- Published
- 2020
43. ZNF185 is a p63 target gene critical for epidermal differentiation and squamous cell carcinoma development
- Author
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Simone Bischetti, Artem Smirnov, Eleonora Candi, Anna Maria Lena, Angela Cappello, Emanuele Panatta, Alessandro Mauriello, Gennaro Melino, Margherita Annicchiarico-Petruzzelli, Lucia Anemona, Smirnov, Artem [0000-0002-1575-8725], Mauriello, Alessandro [0000-0002-7351-5676], and Apollo - University of Cambridge Repository
- Subjects
Keratinocytes ,0301 basic medicine ,Cancer Research ,Cellular differentiation ,Down-Regulation ,Settore MED/08 ,Biology ,Models, Biological ,Article ,03 medical and health sciences ,0302 clinical medicine ,Antigens, CD ,Cell Adhesion ,Genetics ,Adherens junctions ,Humans ,Gene silencing ,Settore BIO/10 ,Enhancer ,Cell adhesion ,Molecular Biology ,Transcription factor ,Cells, Cultured ,Regulation of gene expression ,Sequence Analysis, RNA ,Cell adhesion molecule ,Oral cancer ,Gene Expression Profiling ,Tumor Suppressor Proteins ,Cell Differentiation ,Epithelial Cells ,LIM Domain Proteins ,Cadherins ,Cell biology ,Gene Expression Regulation, Neoplastic ,Gene expression profiling ,Cytoskeletal Proteins ,Enhancer Elements, Genetic ,030104 developmental biology ,Differentiation ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Transcription Factors - Abstract
Development and maintenance of healthy stratified epithelia require the coordination of complex transcriptional programmes. The transcription factor p63, a member of the p53 family, plays a crucial role in epithelial development and homeostasis. Analysis of the p63-dependent transcriptome indicated that one important aspect of p63 functions in epithelial development is the regulation of cell–cell and cell–matrix adhesion programmes. However, limited knowledge exists on the relevant cell–cell adhesion molecules involved in physiological epithelial formation. Similarly, limited data are available to understand if deregulation of the cell–cell adhesion programme is important in tumour formation. Here, using the epidermis as an experimental model with the RNA sequencing approach, we identify a novel p63-regulated gene induced during differentiation, ZNF185. ZNF185 is an actin-cytoskeleton-associated Lin-l 1, Isl-1 and Mec-3 (LIM) domain-containing protein, whose function is poorly known. We found that p63 binds to a specific enhancer region, promoting its expression to sustain epithelial differentiation. ZNF185 silencing strongly impaired keratinocyte differentiation according to gene array analysis. ZNF185 is detected at the cell–cell periphery where it physically interacts with E-cadherin, indicating that it is important to maintain epithelial integrity beyond its pro-differentiation role. Interestingly, poorly differentiated, including head and neck, cervical and oesophageal, squamous cell carcinomas display loss of ZNF185 expression. Together, these studies reinforce that p63 is a crucial gene for maintaining epithelial tissue integrity and support the deregulation of the cell-cell adhesion programme,which plays a critical role in carcinoma development.
- Published
- 2018
44. Reducing the Number of Unnecessary Percutaneous Biopsies: The Role of Second Opinion by Expert Breast Center Radiologists
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Alberto Collura, Daniela Tosti, Feliciana Lamacchia, Francesca Ricci, Marco Materazzo, Michela Censi, Gianluca Vanni, Tommaso Perretta, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Francesca Di Giuliano, and Lucia Anemona
- Subjects
Core needle ,Data Analysis ,Image-Guided Biopsy ,Cancer Research ,medicine.medical_specialty ,Percutaneous ,Biopsy ,Biopsy, Fine-Needle ,Clinical Decision-Making ,breast imaging reporting and data system ,Breast Neoplasms ,Fine needle aspiration cytology ,breast lesions ,Tru-Cut needle biopsy ,breast cancer ,cancer ,multidisciplinary team ,tertiary care center ,Biopsy, Large-Core Needle ,Disease Management ,Expert Testimony ,Female ,Humans ,Mammography ,Radiologists ,Referral and Consultation ,Workflow ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Settore MED/36 ,medicine ,Family history ,skin and connective tissue diseases ,medicine.diagnostic_test ,business.industry ,Second opinion ,Cancer ,General Medicine ,medicine.disease ,Fine-needle aspiration ,Oncology ,030220 oncology & carcinogenesis ,Fine-Needle ,Large-Core Needle ,Radiology ,business - Abstract
BACKGROUND/AIM The research objectives of this study were the estimation of the number of misdiagnosed breast lesions by non-expert-center-breast-radiologists (NEBR) and the investigation of the discordant rate (DR) calculated between initial and second opinion. Moreover, this study evaluated the impact of second opinion and the factors associated with DR. MATERIALS AND METHODS A total of 399 patients were sent to our Tertiary Breast Cancer (BC) Center to perform fine needle aspiration/core needle biopsy (FNAC/CNB) after external examination. Lesions were reclassified according to Breast Imaging-Reporting and Data System (BI-RADS). External examinations were classified as breast-expert, not-breast-expert and physicians as expert-center-breast-radiologists (EBR), NEBR, and non-radiologists (NR). Personal/family history of breast cancer (BC), breast-density and presence of prior imaging were collected. RESULTS DR was 74.3%. After second opinion, FNAC/CNB was proposed in 25.7% of cases and 2 additional cancers were detected. About 59.5% of unnecessary FNAC/CNB were avoided. Dense breast, no prior imaging examination and BC family-history were associated with higher DR (p-value
- Published
- 2019
45. Unilateral Maculopapular Eruption in a Child: Answer
- Author
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Maria Vittoria Cannizzaro, Andrea Saggini, Luca Bianchi, Augusto Orlandi, and Lucia Anemona
- Subjects
medicine.medical_specialty ,Settore MED/35 ,Text mining ,business.industry ,medicine ,Dermatology ,General Medicine ,business ,Pathology and Forensic Medicine - Published
- 2021
46. A case of metachronous peripheral T‐Cell non‐Hodgkin lymphoma following chemotherapy for Hodgkin disease successfully treated with brentuximab vedotin
- Author
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Meconi, Federico, primary, Provenzano, Ida, additional, Nasso, Daniela, additional, Mariotti, Benedetta, additional, Pupo, Livio, additional, Secchi, Roberto, additional, Cerretti, Raffaella, additional, Lucia, Anemona, additional, Arcese, William, additional, and Cantonetti, Maria, additional
- Published
- 2020
- Full Text
- View/download PDF
47. Histological features of bone marrow in paediatric patients during the asymptomatic phase of early-stage Black African sickle cell anaemia
- Author
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Simone Facchetti, Antonella Isgrò, Alessandro Mauriello, Lucia Anemona, Andrea Saggini, and Erica Giacobbi
- Subjects
Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Myeloid ,Adolescent ,Settore MED/08 ,Bone Marrow Cells ,Anemia, Sickle Cell ,Asymptomatic ,Pathology and Forensic Medicine ,Sickle cell anaemia ,electron microscopy ,pathology ,Bone Marrow ,Child ,Child, Preschool ,Female ,Hematopoietic Stem Cell Transplantation ,Humans ,Myelodysplastic Syndromes ,Spleen ,03 medical and health sciences ,medicine ,Preschool ,business.industry ,Anemia ,Erythroid Hyperplasia ,Pathophysiology ,Sickle Cell ,Transplantation ,Haematopoiesis ,030104 developmental biology ,medicine.anatomical_structure ,Bone marrow ,Stem cell ,medicine.symptom ,business - Abstract
Bone marrow histological features of sickle cell anaemia (SCA) patients during early stages and in the asymptomatic phase of the disease appear an interesting area of study, representing early-stage consequences of SCA with a close relation to its pathophysiology. Unfortunately, this field of research has never been specifically addressed before. Bone marrow biopsies from 26 consecutive Black African SCA patients (M:F=1.6:1; age 2-17 years), free of clinical signs of chronic bone marrow damage, with no recent history of symptomatic vaso-occlusive episodes, and waiting for haematopoietic stem cell transplantation (HSCT), underwent morphological, immunohistochemical and electron microscopy evaluation. Additional comparison with three bone marrow specimens from post-HSCT SCA patients and 10 bone marrow specimens from AS healthy carriers was performed. Bone marrow of SCA patients was normocellular or slighly hypercellular in all cases. Erythroid hyperplasia was a common feature. Myeloid lineage was slightly decreased with normal to slightly diminished neutrophilic granulocytes; CD68 positive monocytic-macrophagic cells appeared slightly increased, with a predominant CD163 positive M2/M(Hb) phenotype. A positive correlation was found between haemoglobin values and number of bone marrow erythroid cells (R2=0.15, p=0.05). Intravascular and interstitial clusters of erythroid sickle cells were found in bone marrow of pre-HSCT homozygous SS SCA patients, as well as heterozygous AS healthy carriers, and the single post-HSCT patient matched to an AS health carrier donor.
- Published
- 2017
48. The Efficacy and Safety of Lenalidomide plus Rituximab in an Orbital Relapse of Diffuse Large B-Cell Lymphoma
- Author
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G. Paterno, Lucia Anemona, Federico Meconi, Daniela Nasso, Vito Mario Rapisarda, Sara Vaccarini, Maria Cantonetti, Livio Pupo, Fabiana Esposito, and Raffaele Palmieri
- Subjects
Oncology ,medicine.medical_specialty ,medicine.medical_treatment ,Case Report ,03 medical and health sciences ,0302 clinical medicine ,Maintenance therapy ,Blurred vision ,Internal medicine ,hemic and lymphatic diseases ,medicine ,Stage (cooking) ,Lenalidomide ,lcsh:RC633-647.5 ,business.industry ,lcsh:Diseases of the blood and blood-forming organs ,General Medicine ,Settore MED/15 ,medicine.disease ,Lymphoma ,Radiation therapy ,030220 oncology & carcinogenesis ,Rituximab ,medicine.symptom ,business ,Diffuse large B-cell lymphoma ,030215 immunology ,medicine.drug - Abstract
A 74-year-old male with diffuse large B-cell lymphoma, with an Ann Arbor stage IV-A, was submitted to immune-chemotherapy in 2014, with complete remission of the disease. Two years later, he presented with a left eye swelling leading to exophthalmos and blurred vision. A core biopsy was performed and revealed a local relapse of the disease. He was considered unfit for intensive salvage chemotherapy and was treated with a combination of rituximab and lenalidomide. After six courses of rituximab plus lenalidomide, the patient showed complete remission and was submitted to maintenance therapy with lenalidomide. After 24 months since the start of lenalidomide monotherapy, we did not observe any progression. In this experience, rituximab plus lenalidomide, without radiotherapy, was a safe and effective therapeutic combination in an elderly patient with a localized relapse of DLBCL who was unfit to receive more aggressive therapies.
- Published
- 2019
49. ZNF750 represses breast cancer invasion via epigenetic control of prometastatic genes
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Matteo Cassandri, Alessandro Mauriello, Manuela Montanaro, Massimiliano Agostini, Anna Maria Lena, Alessio Butera, Gerry Melino, Ivano Amelio, Lucia Anemona, Richard A. Knight, and Eleonora Candi
- Subjects
0301 basic medicine ,Transcriptional Activation ,Cancer Research ,Datasets as Topic ,Golgi Apparatus ,Breast Neoplasms ,Histone Deacetylase 1 ,Biology ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Cell Movement ,Cell Line, Tumor ,Protein Interaction Mapping ,Genetics ,medicine ,Histone code ,Humans ,Neoplasm Invasiveness ,Epigenetics ,Neoplasm Metastasis ,Promoter Regions, Genetic ,Molecular Biology ,Wnt Signaling Pathway ,Regulation of gene expression ,Histone Demethylases ,Focal Adhesions ,Binding Sites ,Settore BIO/11 ,Tumor Suppressor Proteins ,Cancer ,Cell Polarity ,KDM1A ,medicine.disease ,Prognosis ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,Histone Code ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Female ,Cell Adhesion Molecules ,alpha Catenin ,Transcription Factors - Abstract
Breast cancer is the second leading cause of cancer-related deaths among women, largely due to the progression of a significant fraction of primary tumours to the metastatic stage. Here, we show that zinc-finger protein 750 (ZNF750) opposes the migration and invasion of breast cancer cells by repressing a prometastatic transcriptional programme, which includes genes involved in focal adhesion and extracellular matrix interactions, such as LAMB3 and CTNNAL1. Mechanistically, ZNF750 recruits the epigenetic modifiers KDM1A and HDAC1 to the promoter regions of LAMB3 and CTNNAL1, influencing histone marks and transactivating these genomic sites. Gene expression analysis in cancer patient datasets indicated that ZNF750 and its targets were negative prognostic factors in breast cancer. Together, our findings shed light on the molecular mechanism by which ZNF750 regulates cell migration and invasion, suggesting a role in breast cancer metastasis.
- Published
- 2019
50. Morphological evaluation of sympathetic renal innervation in patients with autosomal dominant polycystic kidney disease
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Nicola Di Daniele, Alessandro Mauriello, Adriana D'Ercole, Pierluigi Bove, Giuseppe Santeusanio, Silvestro Mauriello, Annalisa Noce, Elena Bonanno, Erica Giacobbi, Manuel Scimeca, Gabriele D'Urso, Alessandro Anselmo, Maurizio Casasco, Valentina Rovella, Lucia Anemona, and Elena Giannini
- Subjects
ADPKD ,Histology ,Renal denervation ,Sympathetic renal hyperactivity ,Adult ,Male ,medicine.medical_specialty ,Adventitia ,Sympathetic Nervous System ,medicine.medical_treatment ,030232 urology & nephrology ,Urology ,Autosomal dominant polycystic kidney disease ,Kidney Volume ,Settore MED/08 - Anatomia Patologica ,030204 cardiovascular system & hematology ,urologic and male genital diseases ,Nephrectomy ,03 medical and health sciences ,0302 clinical medicine ,Renal Artery ,medicine.artery ,medicine ,Humans ,In patient ,Renal artery ,Aged ,Aged, 80 and over ,Kidney ,business.industry ,Middle Aged ,medicine.disease ,Polycystic Kidney, Autosomal Dominant ,medicine.anatomical_structure ,Nephrology ,Case-Control Studies ,Hypertension ,Immunohistochemistry ,Female ,business - Abstract
Several evidences support the hypothesis that patients affected by autosomal dominant polycystic kidney disease (ASPKD) show a sympathetic renal hyperactivity. Nevertheless, no morphological evidences are available yet. Therefore, the aim of the study was to demonstrate that an increase in sympathetic renal artery innervation was present in the ADPKD patients by using histological methods. In addition, here we correlated the sympathetic renal artery innervation with the evolutionary state of ADPKD (increase in volume of kidney, onset of chronic renal failure and hypertension). To this end, peri-adventitial innervation of renal arteries was studied using morphological methods from 49 patients in total: 29 underwent surgical nephrectomies for ADPKD and 20 non-dialysis patients (CTRL group) undergoing nephrectomy for other diseases. Nerve density (number of nerves per mm2) was evaluated in the peri-adventitial tissue in a concentric ring that was located within 2 mm from the beginning of the adventitia by using immunohistochemistry. The total nerve density was significantly increased in the ADPKD group (1.26 ± 0.82 × mm2) as compared to controls (0.78 ± 0.40 × mm2) (p = 0.02). Hypertensive patients with ADPKD showed a greater nerve density than control hypertensives. However, the increase in renal sympathetic innervation in the ADPKD patients was found to be independent of hypertension, resistance to antihypertensive therapy, age, sex and kidney volume, as demonstrated by the uni and multivariate analysis. In conclusion, our study better clarifies the effect of sympathetic hyperactivity in the progression of polycystic disease.
- Published
- 2019
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