102 results on '"Lozano-Sánchez J"'
Search Results
2. Contributors
- Author
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Aligiannis, Nektarios, primary, Amirante, Riccardo, additional, Argyropoulou, Aikaterini, additional, Bonvino, Natalie P., additional, Boskou, Dimitrios, additional, Camposeo, Salvatore, additional, Carretero, A. Segura, additional, Cicero, Nicola, additional, Clodoveo, Maria Lisa, additional, Gámez, M.d.M. Contreras, additional, Covas, María-Isabel, additional, Dais, Photis, additional, Dugo, Giacomo, additional, Fitó, Montserrat, additional, Gómez-Caravaca, A.M., additional, Halabalaki, Maria, additional, Hatzakis, Emmanuel, additional, Kaliora, Andriana C., additional, Kalogeropoulos, Nick, additional, Karagiannis, Tom C., additional, Lam, Nicholas T., additional, Lozano-Sánchez, J., additional, Luc, Raymond, additional, Malheiro, Ricardo, additional, Pereira, José Alberto, additional, Ray, Nancy B., additional, Rodrigues, Nuno, additional, Skaltsounis, Alexios-Leandros, additional, Taamalli, A., additional, Termentzi, Aikaterini, additional, de la Torre, Rafael, additional, and Xynos, Nikos, additional
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- 2015
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3. Bioactive Phenolic Compounds from Olea europaea: A Challenge for Analytical Chemistry
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Gómez-Caravaca, A.M., primary, Lozano-Sánchez, J., additional, Gámez, M.d.M. Contreras, additional, Carretero, A. Segura, additional, and Taamalli, A., additional
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- 2015
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4. Characterization of a new blackberry cultivar BRS Xingu: chemical composition, phenolic compounds, and antioxidant capacity in vitro and in vivo
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MORAES, D. P., LOZANO-SÁNCHEZ, J., MACHADO, M. L., VIZZOTTO, M., LAZZARETTI, M., LEYVA-JIMENEZ, F. J. J., SILVEIRA, T. da, RIES, E. F., BARCIA, M. T., DÉBORA P. MORAES, UFSM, JESÚS LOZANO-SÁNCHEZ, UNIVERSITY OF GRANADA, MARINA L. MACHADO, UFSM, MARCIA VIZZOTTO, CPACT, MICHELI LAZZARETTI, UFSM, FRANCISCO JAVIER J. LEYVA-JIMENEZ, FUNCTIONAL FOOD RESEARCH AND DEVELOPMENT CENTER, HEALTH SCIENCE TECHNOLOGY PARK, TÁSSIA L. DA SILVEIRA, UFSM, EDI F. RIES, UFSM, and MILENE T. BARCIA, UFSM.
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Amora Preta ,Cultivar BRS Xingu - Abstract
The cultivar BRS Xingu was launched by EMBRAPA in 2015 with the intention of presenting higher productivity. Due to the lack of studies on this cultivar, the objective was to present the physical?chemical, centesimal, and phenolic composition of the BRS Xingu blackberry, its antioxidant capacity, protection against ROS generation, and compare it with other commercialized cultivars such as Guarani, Tupy, and Xavante. The BRS Xingu was prominent regarding anthocyanin and condensed tannin content and superior to the other cultivars. Moreover, BRS Xingu presented higher antioxidant capacity, protection of C. elegans from ROS generation, and soluble solid content when compared to Tupy, which is the most cultivated variety in the world. In the new cultivar, five anthocyanins, five phenolic acids, and ten non-anthocyanin flavonoids were identified. BRS Xingu is presented as an alternative blackberry with potential for industrialization and in natura consumption. Made available in DSpace on 2020-12-04T09:05:42Z (GMT). No. of bitstreams: 1 5-MORAES.pdf: 842101 bytes, checksum: 7bf4a8730887faf3e90dccf3de2e6748 (MD5) Previous issue date: 2020
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- 2020
5. Effect of microwave hydrodiffusion and gravity on the extractionof phenolic compounds and antioxidant properties of blackberries (Rubusspp.): scale-Up extraction
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MORAES, D. P., MACHADO, M. L., FARIAS, C. A. A., BARIN, J. S., ZABOT, G. L., LOZANO-SÁNCHEZ, J., FERREIRA, d. f., VIZZOTTO, M., LEYVA-JIMENEZ, F. J., SILVEIRA, T. L. DA, RIES, E. F., BARCIA, M. T., DÉBORA P. MORAES, UNIVERSIDADE FEDERAL DE SANTA MARIA, MARINA L. MACHADO, UNIVERSIDADE FEDERAL DE SANTA MARIA, CARLA A. A. FARIAS, UNIVERSIDADE FEDERAL DE SANTA MARIA, JULIANO S. BARIN, UNIVERSIDADE FEDERAL DE SANTA MARIA, GIOVANI L. ZABOT, UNIVERSIDADE FEDERAL DE SANTA MARIA, JESÚS LOZANO-SÁNCHEZ, HEALTH SCIENCE TECHNOLOGY PARK, FUNCTIONAL FOOD RESEARCH ANDDEVELOPMENT CENTER, GRANADA, SPAIN, DANIELE F. FERREIRA, UNIVERSIDADE FEDERAL DE SANTA MARIA, MARCIA VIZZOTTO, CPACT, FRANCISCO JAVIER LEYVA-JIMENEZ, HEALTH SCIENCE TECHNOLOGY PARK, FUNCTIONAL FOOD RESEARCH ANDDEVELOPMENT CENTER, GRANADA, SPAIN, TÁSSIA L. DA SILVEIRA, UNIVERSIDADE FEDERAL DE SANTA MARIA, EDI F. RIES, UNIVERSIDADE FEDERAL DE SANTA MARIA, and MILENE T. BARCIA, UNIVERSIDADE FEDERAL DE SANTA MARIA.
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Blackberries ,Antioxidante ,Amora Preta ,Composto Fenólico - Abstract
The phenolic compounds of blackberries extracted with organic solvents cause environmental damage. Therefore, the objective of thepresent study was to verify if microwave hydrodiffusion and gravity obtain a blackberry extract with a high concentration of phenoliccompounds and antioxidant capacity without the addition of any solvent. The results showed that it was possible to reach the objectivewith 500 W and 10 min of extraction by employing a method that meets green chemistry principles. The extract has a lower cost thanthe exhaustive method, is microbiologically safe, and is mainly composed of anthocyanins (85%). The presence of 5 anthocyanins and17 non-anthocyanin phenolic compounds were identified, including hydroxyresveratrol, which was first extracted in blackberries bymicrowave hydrodiffusion and gravity. The phenolic compound content and antioxidant capacity were lower in the last fractions,which reduced the extraction time to 8 min. The coproduct showed phenolic, antioxidant capacity, and microbiological quality. Thisstudy presented a fast, efficient, economical, sustainable, and solvent-free method to extract phenolic compounds from blackberries. Made available in DSpace on 2020-12-04T09:04:16Z (GMT). No. of bitstreams: 1 1-MORAES.pdf: 2205348 bytes, checksum: 1842695e7546a6cc52e0f207be127845 (MD5) Previous issue date: 2020 Published online: 20 November 2020.
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- 2020
6. Pressurized GRAS solvents for the green extraction of phenolic compounds from hibiscus sabdariffa calyces
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Pimentel-Moral, S., primary, Borrás-Linares, I., additional, Lozano-Sánchez, J., additional, Alañón, M.E., additional, Arráez-Román, D., additional, and Segura-Carretero, A., additional
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- 2020
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7. Extra-virgin olive oil contains a metabolo-epigenetic inhibitor of cancer stem cells
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Universitat Rovira i Virgili, Corominas-Faja B; Cuyàs E; Lozano-Sánchez J; Cufí S; Verdura S; Fernández-Arroyo S; Borrás-Linares I; Martin-Castillo B; Martin Á; Lupu R; Nonell-Canals A; Sanchez-Martinez M; Micol V; Joven J; Segura-Carretero A; Menendez J, Universitat Rovira i Virgili, and Corominas-Faja B; Cuyàs E; Lozano-Sánchez J; Cufí S; Verdura S; Fernández-Arroyo S; Borrás-Linares I; Martin-Castillo B; Martin Á; Lupu R; Nonell-Canals A; Sanchez-Martinez M; Micol V; Joven J; Segura-Carretero A; Menendez J
- Abstract
Targeting tumor-initiating, drug-resistant populations of cancer stem cells (CSC) with phytochemicals is a novel paradigm for cancer prevention and treatment. We herein employed a phenotypic drug discovery approach coupled to mechanism-of-action profiling and target deconvolution to identify phenolic components of extra virgin olive oil (EVOO) capable of suppressing the functional traits of CSC in breast cancer (BC). In vitro screening revealed that the secoiridoid decarboxymethyl oleuropein aglycone (DOA) could selectively target subpopulations of epithelial-like, aldehyde dehydrogenase (ALDH)-positive and mesenchymal-like, CD44+CD24-/low CSC. DOA could potently block the formation of multicellular tumorspheres generated from single-founder stem-like cells in a panel of genetically diverse BC models. Pretreatment of BC populations with noncytotoxic doses of DOA dramatically reduced subsequent tumor-forming capacity in vivo. Mice orthotopically injected with CSC-enriched BC-cell populations pretreated with DOA remained tumor-free for several months. Phenotype microarray-based screening pointed to a synergistic interaction of DOA with the mTOR inhibitor rapamycin and the DNA methyltransferase (DNMT) inhibitor 5-azacytidine. In silico computational studies indicated that DOA binds and inhibits the ATP-binding kinase domain site of mTOR and the S-adenosyl-l-methionine (SAM) cofactor-binding pocket of DNMTs. FRET-based Z-LYTE™ and AlphaScreen-based in vitro assays confirmed the ability of DOA to function as an ATP-competitive mTOR inhibitor and to block the SAM-dependent methylation activity of DNMTs. Our systematic in vitro, in vivo and in silico approaches establish the phenol-conjugated oleoside DOA as a dual mTOR/DNMT inhibitor naturally occurring in EVOO that functiona
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- 2018
8. AMPK modulatory activity of olive-tree leaves phenolic compounds: Bioassay-guided isolation on adipocyte model and in silico approach
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Jiménez-Sánchez C, Olivares-Vicente M, Rodríguez-Pérez C, Herranz-López M, Lozano-Sánchez J, Segura-Carretero A, Fernández-Gutiérrez A, Encinar JA, and Micol V
- Abstract
Scope Olive-tree polyphenols have demonstrated potential for the management of obesity-related pathologies. We aimed to explore the capacity of Olive-tree leaves extract to modulate triglyceride accumulation and AMP-activated protein kinase activity (AMPK) on a hypertrophic adipocyte model. Methods Intracellular triglycerides and AMPK activity were measured on the hypertrophic 3T3-L1 adipocyte model by AdipoRed and immunofluorescence microscopy, respectively. Reverse phase high performance liquid chromatography coupled to time-of-flight mass detection with electrospray ionization (RP-HPLC-ESI-TOF/MS) was used for the fractionation of the extract and the identification of the compounds. In-silico molecular docking of the AMPK alpha-2, beta and gamma subunits with the identified compounds was performed. Results Olive-tree leaves extract decreased the intracellular lipid accumulation through AMPK-dependent mechanisms in hypertrophic adipocytes. Secoiridoids, cinnamic acids, phenylethanoids and phenylpropanoids, flavonoids and lignans were the candidates predicted to account for this effect. Molecular docking revealed that some compounds may be AMPK-gamma modulators. The modulatory effects of compounds over the alpha and beta AMPK subunits appear to be less probable. Conclusions Olive-tree leaves polyphenols modulate AMPK activity, which may become a therapeutic aid in the management of obesity-associated disturbances. The natural occurrence of these compounds may have important nutritional implications for the design of functional ingredients.
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- 2017
9. Cocoa and grape seed byproducts as a source of antioxidant and anti-inflammatory proanthocyanidins
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Universitat Rovira i Virgili, Cádiz-Gurrea ML, Borrás-Linares I, Lozano-Sánchez J, Joven J, Fernández-Arroyo S, Segura-Carretero A, Universitat Rovira i Virgili, and Cádiz-Gurrea ML, Borrás-Linares I, Lozano-Sánchez J, Joven J, Fernández-Arroyo S, Segura-Carretero A
- Abstract
Phenolic compounds, which are secondary plant metabolites, are considered an integral part of the human diet. Physiological properties of dietary polyphenols have come to the attention in recent years. Especially, proanthocyanidins (ranging from dimers to decamers) have demonstrated potential interactions with biological systems, such as antiviral, antibacterial, molluscicidal, enzyme-inhibiting, antioxidant, and radical-scavenging properties. Agroindustry produces a considerable amount of phenolic-rich sources, and the ability of polyphenolic structures to interacts with other molecules in living organisms confers their beneficial properties. Cocoa wastes and grape seeds and skin byproducts are a source of several phenolic compounds, particularly mono-, oligo-, and polymeric proanthocyanidins. The aim of this work is to compare the phenolic composition of Theobroma cacao and Vitis vinifera grape seed extracts by high pressure liquid chromatography coupled to a quadrupole time-of-flight mass spectrometer and equipped with an electrospray ionization interface (HPLC-ESI-QTOF-MS) and its phenolic quantitation in order to evaluate the proanthocyanidin profile. The antioxidant capacity was measured by different methods, including electron transfer and hydrogen atom transfer-based mechanisms, and total phenolic and flavan-3-ol contents were carried out by Folin-Ciocalteu and Vanillin assays. In addition, to assess the anti-inflammatory capacity, the expression of MCP-1 in human umbilical vein endothelial cells was measured.
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- 2017
10. Effects of ultrasounds treatment integrated in a low-scale plant on the quality of virgin olive oils: preliminary trials
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VALLI, ENRICO, BENDINI, ALESSANDRA, DI LECCE, GIUSEPPE, GALLINA TOSCHI, TULLIA, Lozano Sánchez J., Segura Carretero A., Valli E., Bendini A., Di Lecce G., Lozano Sánchez J., Segura Carretero A., and Gallina Toschi T.
- Abstract
The application of an innovative ultrasounds (US) treatment, fully integrated in a low-scale plant for the production of virgin olive oils was studied. In particular, a prototyped US generator (500 W/L, effective ultrasonic power and 25 kHz, frequency), coupled with a tube-shaped transducer (2.25 L as internal capacity) and a screw-pump, was placed between the malaxator and the two-phases decanter. Two experimental trials were performed by processing separately 60 kg batches of destoned olives from different cultivars, collected in Italy during 2013. The effects of the US on the sensory and chemical quality parameters of the virgin olive oils were evaluated, by comparing the results of the two treated samples (U1, U2) with the respective ones, obtained extracting the oil using the same plant described above, but without the application of the US (C1, C2). The results showed that the application of such a treatment did not affect the basic quality parameters of the oils (free acidity, peroxide values, UV extinction coefficients), but increased the oil yield in respect with the control samples. On the other hand, a decrease of the oxidative stability (OSI test) of both the treated samples was registered, probably due to a reduction in the antioxidant fraction, mainly in terms of the dialdehydic form of ligstroside aglycone and -carotene, whereas the content in -tocopherol seems to be less susceptible. The application of the US treatment led also to a significant increase in the micro-dispersed water content, maybe due to the cavitation phenomena. This higher amount of water probably facilitates the transfer in the oil matrix of microorganisms/enzymes responsible for fermentation/degradation of sugars/protein material. This could explain the higher content of polar volatiles (ethanol, 3-methyl-1-butanol, ethyl acetate, acetic acid) markers of sensory defects, such as winey/fusty, in particular for U1, the richest in water. This study resumes the observations of some preliminary trials in order to verify if this innovative technology could be considered useful for an industrial scale application with a preservation of the product quality. Further tests have to be carry out to find convenient processing parameters.
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- 2014
11. Characterisation of the phenolic compounds retained in different organic and inorganic filter aids used for filtration of extra virgin olive oil
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Lozano-Sánchez, J., primary, Segura-Carretero, A., additional, and Fernández-Gutiérrez, A., additional
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- 2011
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12. Phenolic secoiridoids in extra virgin olive oil impede fibrogenic and oncogenic epithelial-to-mesenchymal transition: extra virgin olive oil as a source of novel antiaging phytochemicals.
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Vazquez-Martin A, Fernández-Arroyo S, Cufí S, Oliveras-Ferraros C, Lozano-Sánchez J, Vellón L, Micol V, Joven J, Segura-Carretero A, Menendez JA, Vazquez-Martin, Alejandro, Fernández-Arroyo, Salvador, Cufí, Sílvia, Oliveras-Ferraros, Cristina, Lozano-Sánchez, Jesús, Vellón, Luciano, Micol, Vicente, Joven, Jorge, Segura-Carretero, Antonio, and Menendez, Javier A
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- 2012
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13. Filtration process of extra virgin olive oil: effect on minor components, oxidative stability and sensorial and physicochemical characteristics
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Lozano-Sánchez, J., Cerretani, L., Bendini, A., Segura-Carretero, A., and Fernández-Gutiérrez, A.
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OLIVE oil , *FILTERS & filtration , *SUSPENDED solids , *HUMIDITY control , *PHOSPHOLIPIDS , *WAXES , *PHENOLS , *TASTE testing of food - Abstract
The aim of this review is to describe different filtration systems of extra virgin olive oil (EVOO): a) to remove suspended solids, b) to eliminate humidity and making the oil brilliant, c) cross-flow filtration and d) new filtration systems based on filter bag and the flow of an inert gas. During filtering operations quantitative and qualitative changes take place, especially on minor components, which are of great value in establishing of the quality of EVOO. The present review focuses on the effect of these different filtration systems in the minor fraction of EVOO with particular emphasis on waxes, carbonyl compounds, pigments, lipophilic and hydrophilic phenols, phospholipids and proteins. [Copyright &y& Elsevier]
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- 2010
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14. A new extraction approach to correct the effect of apparent increase in the secoiridoid content after filtration of virgin olive oil
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Cristiano Augusto Ballus, Jesús Lozano-Sánchez, Abdelhakim Bakhouche, Alberto Fernández-Gutiérrez, Antonio Segura-Carretero, Alessandra Bendini, Tullia Gallina-Toschi, Bakhouche A., Lozano-Sánchez J., Ballus C. A., Bendini A., Gallina Toschi T., Fernández-Gutiérrez A., and Segura-Carretero A.
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Chromatography ,Moisture ,Chemistry ,HPLC-ESI-TOF/MS ,Industrial scale ,Extraction (chemistry) ,Internal Standard ,VOO ,Fraction (chemistry) ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,Oleuropein ,law ,FILTRATION ,PHENOLIC COMPOUNDS ,Water content ,Filtration ,Olive oil - Abstract
In the current study, a new approach has been developed for correcting the effect that moisture reduction after virgin olive oil (VOO) filtration exerts on the apparent increase of the secoiridoid content by using an internal standard during extraction. Firstly, two main Spanish varieties (Picual and Hojiblanca) were submitted to industrial filtration of VOOs. Afterwards, the moisture content was determined in unfiltered and filtered VOOs, and liquid-liquid extraction of phenolic compounds was performed using different internal standards. The resulting extracts were analyzed by HPLC-ESI-TOF/MS, in order to gain maximum information concerning the phenolic profiles of the samples under study. The reduction effect of filtration on the moisture content, phenolic alcohols, and flavones was confirmed at the industrial scale. Oleuropein was chosen as internal standard and, for the first time, the apparent increase of secoiridoids in filtered VOO was corrected, using a correction coefficient (Cc) calculated from the variation of internal standard area in filtered and unfiltered VOO during extraction. This approach gave the real concentration of secoiridoids in filtered VOO, and clarified the effect of the filtration step on the phenolic fraction. This finding is of great importance for future studies that seek to quantify phenolic compounds in VOOs.
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- 2014
15. Misdescription of edible oils: Flowcharts of analytical choices in a forensic view
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Alessandra Bendini, Antonio Segura-Carretero, Tullia Gallina Toschi, Lanfranco S. Conte, Jesús Lozano-Sánchez, Gallina Toschi T., Bendini A., Lozano-Sánchez J., Segura-Carretero A., and Conte L.
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Flowchart ,Computer science ,Process (engineering) ,media_common.quotation_subject ,virgin oils ,General Chemistry ,sensorial analysis ,Data science ,forensic approach to food analysis ,Industrial and Manufacturing Engineering ,law.invention ,Forensic science ,authenticity ,virgin oil ,Chemical marker ,origin ,Forensic approach in food analysis ,law ,Quality (business) ,sensory analysi ,Food Science ,Biotechnology ,media_common - Abstract
The first commercial differentiation of edible oils, from a technological point of view, distinguishes them as non-refined (e.g., virgin) and refined, due to the higher commercial value of the former. From this basic quality originates one of the most diffused oils' fraud, the misdeclaration of one or all the phases of the refining process (i). However there are many other ways in which an oil can be misdescribed, such as by: (ii) substituting all or a part of it with a similar but cheaper oil and (iii) declaring a false geographical or botanical origin. The present review addresses the problem of authenticity and misdescriptions of the oils (i–iii) through analytical schemes that can be followed and then used in a forensic view, to provide clues or evidence of fraud. It mentions official methods, known and innovative approaches, from chromatographic or derived methods to spectrometric, spectroscopic, DNA-based, thermogravimetric, and calorimetric procedures for discriminating genuine from defrauded olive and seed oils. Practical applications: The forensic use of a chemical marker of a fraud, or of an analytical method is questionable if a legal limit or range is not yet defined, or when an official procedure has not been published at least in one State. On the other hand, a validated procedure or evidence can be used for legal purposes when it is possible to demonstrate that the scientific knowledge is more advanced than the existing official methods. In this paper known or promising methods, to discover and prove the most common misdescription of edible oils, which could be useful for a forensic application, are reported and reviewed.
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- 2013
16. Corrigendum to "Influence of the crystallinity on the physicochemical properties of spray-dried quercetin-inulin microparticles and their performance during in vitro digestion" [Food Chemistry Volume 434 (2024) 137325].
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Quintriqueo-Cid A, Giménez B, Romero-Hasler P, Soto-Bustamante E, Lozano-Sánchez J, and Robert P
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- 2024
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17. Mass Spectrometry Characterization of Honeydew Honey: A Critical Review.
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Quirantes-Piné R, Sanna G, Mara A, Borrás-Linares I, Mainente F, Picó Y, Zoccatelli G, Lozano-Sánchez J, and Ciulu M
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Honeydew honey is produced by bees ( Apis mellifera ) foraging and collecting secretions produced by certain types of aphids on various parts of plants. In addition to exhibiting organoleptic characteristics that distinguish them from nectar honey, these honeys are known for their functional properties, such as strong antioxidant and anti-inflammatory activities. Despite their importance, they remain poorly characterized in comparison with flower honeys, as most studies on this subject are not only carried out on too few samples but also still focused on traditional chemical-physical parameters, such as specific rotation, major sugars, or melissopalynological information. Since mass spectrometry has consistently been a primary tool for the characterization and authentication of honeys, this review will focus on the application of these methods to the characterization of the minor fraction of honeydew honey. More specifically, this review will attempt to highlight what progress has been made so far in identifying markers of the authenticity of the botanical and/or geographical origin of honeydew honeys by mass spectrometry-based approaches. Furthermore, strategies devoted to the determination of contaminants and toxins in honeydew honeys will be addressed. Such analyses represent a valuable tool for establishing the level of food safety associated with these products. A critical analysis of the presented studies will identify their limitations and critical issues, thereby describing the current state of research on the topic.
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- 2024
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18. Monitoring the Phenolic and Terpenic Profile of Olives, Olive Oils and By-Products throughout the Production Process.
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López-Salas L, Díaz-Moreno J, Ciulu M, Borrás-Linares I, Quirantes-Piné R, and Lozano-Sánchez J
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Olive oil is a food of great importance in the Mediterranean diet and culture. However, during its production, the olive oil industry generates a large amount of waste by-products that can be an important source of bioactive compounds, such as phenolic compounds and terpenes, revalorizing them in the context of the circular economy. Therefore, it is of great interest to study the distribution and abundance of these bioactive compounds in the different by-products. This research is a screening focused on phytochemical analysis, with particular emphasis on the identification and quantification of the phenolic and terpenic fractions. Both the main products of the olive industry (olives, olive paste and produced oil) and the by-products generated throughout the oil production process (leaf, "alpeorujo", liquid and solid residues generated during decanting commonly named "borras" and washing water) were analyzed. For this purpose, different optimized extraction procedures were performed for each matrix, followed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-TOF/MS) analysis. Although no phenolic alcohols were quantified in the leaf and the presence of secoiridoids was low, this by-product was notable for its flavonoid (720 ± 20 µg/g) and terpene (5000 ± 300 µg/g) contents. "Alpeorujo" presented a complete profile of compounds of interest, being abundant in phenolic alcohols (900 ± 100 µg/g), secoiridoids (4500 ± 500 µg/g) and terpenes (1200 ± 100 µg/g), among others. On the other hand, while the solid residue of the borras was the most abundant in phenolic alcohols (3700 ± 200 µg/g) and secoiridoids (680 ± 20 µg/g), the liquid fraction of this waste was notable for its content of elenolic acid derivatives (1700 ± 100 µg/mL) and phenolic alcohols (3000 ± 300 µg/mL). Furthermore, to our knowledge, this is the first time that the terpene content of this by-product has been monitored, demonstrating that it is an important source of these compounds, especially maslinic acid (120 ± 20 µg/g). Finally, the phytochemical content in wash water was lower than expected, and only elenolic acid derivatives were detected (6 ± 1 µg/mL). The results highlighted the potential of the olive by-products as possible alternative sources of a wide variety of olive bioactive compounds for their revalorization into value-added products.
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- 2024
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19. Exploring the Nutritional and Bioactive Potential of Olive Leaf Residues: A Focus on Minerals and Polyphenols in the Context of Spain's Olive Oil Production.
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Ronca CL, Duque-Soto C, Samaniego-Sánchez C, Morales-Hernández ME, Olalla-Herrera M, Lozano-Sánchez J, and Giménez Martínez R
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Lyophilized plant-origin extracts are rich in highly potent antioxidant polyphenols. In order to incorporate them into food products, it is necessary to protect these phytochemicals from atmospheric factors such as heat, light, moisture, or pH, and to enhance their bioavailability due to their low solubility. To address these challenges, recent studies have focused on the development of encapsulation techniques for antioxidant compounds within polymeric structures. In this study, lyophilized olive leaf extracts were microencapsulated with the aim of overcoming the aforementioned challenges. The method used for the preparation of the studied microparticles involves external ionic gelation carried out within a water-oil (W/O) emulsion at room temperature. HPLC analysis demonstrates a high content of polyphenols, with 90% of the bioactive compounds encapsulated. Meanwhile, quantification by inductively coupled plasma optical emission spectroscopy (ICP-OES) reveals that the dried leaves, lyophilized extract, and microencapsulated form contain satisfactory levels of macro- and micro-minerals (calcium, potassium, sodium). The microencapsulation technique could be a novel strategy to harness the polyphenols and minerals of olive leaves, thus enriching food products and leveraging the antioxidant properties of the polyphenolic compounds found in the lyophilized extract.
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- 2024
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20. Influence of the crystallinity on the physicochemical properties of spray-dried quercetin-inulin microparticles and their performance during in vitro digestion.
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Quintriqueo-Cid A, Giménez B, Romero-Hasler P, Soto-Bustamante E, Lozano-Sánchez J, and Robert P
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- Polyphenols, Temperature, Digestion, Inulin chemistry, Quercetin
- Abstract
Encapsulation of quercetin (Q) with inulin (In) by spray-drying was performed applying a Box-Behnken design where the effect of the inlet air temperature, percentage of inulin crystallite dispersion and Q content were studied on the crystallinity index (CI). Three microparticle systems with CI between 2 % and 20 % (Q-In-2 %, Q-In-12 % and Q-In-20 %) were selected to study the CI effect on Q release during an in vitro digestion. The higher the CI of microparticles, the higher the encapsulation efficiency (76.4 %, Q-In-20 %). Surface quercetin was steadily released during the oral, gastric, and intestinal phases of the digestion. The CI of the microparticles did not influence the Q bioaccessibility values (23.1-29.7 %). The highest Q delivery occurred during the simulated colonic phase (44.4-66.4 %) due to the action of the inulinase. The controlled crystallization in spray-dried microparticles is a promising strategy for the designing of polyphenol-based microparticles with specific delivery properties., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
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- 2024
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21. Evaluation of Olive Leaf Phenolic Compounds' Gastrointestinal Stability Based on Co-Administration and Microencapsulation with Non-Digestible Carbohydrates.
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Duque-Soto C, Leyva-Jiménez FJ, Quirantes-Piné R, López-Bascón MA, Lozano-Sánchez J, and Borrás-Linares I
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- Inulin, Antioxidants, Functional Food, Hexoses, Phenols, Anti-Inflammatory Agents pharmacology, Olea
- Abstract
The large generation of olive by-products has motivated their revalorization into high-added-value products. In this regard, olive leaves pose as an interesting source of bioactive compounds, due to their phenolic content with commonly known antioxidant, anti-inflammatory, and immunomodulatory properties, with potential application in non-communicable diseases. However, their effectiveness and applicability into functional foods is limited by their instability under gastrointestinal conditions. Thus, the development of protective formulations is essential. In this study, the spray-drying encapsulation of a phenolic-rich olive leaf extract with inulin as the encapsulating agent was optimized. Then, the behavior of the free extract under gastrointestinal conditions, its co-administration with the encapsulating agent, and the optimized microencapsulated formulation were studied through an in vitro gastrointestinal digestion process following the INFOGEST protocol. Digestion of the free extract resulted in the degradation of most compounds, whereas this was minimized in the co-administration of the non-encapsulated extract with the encapsulating agent. This protective effect, related to its interaction with inulin, was similar to the microencapsulated formulation. Thus, both approaches, co-administration and microencapsulation with inulin, could be promising strategies for the improvement of the stability of these anti-inflammatory and immunomodulatory compounds under gastrointestinal conditions, enhancing their beneficial effect.
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- 2023
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22. Non-Digestible Carbohydrates: Green Extraction from Food By-Products and Assessment of Their Effect on Microbiota Modulation.
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Expósito-Almellón X, Duque-Soto C, López-Salas L, Quirantes-Piné R, de Menezes CR, Borrás-Linares I, and Lozano-Sánchez J
- Subjects
- Humans, Hexoses, Phenols, Noncommunicable Diseases, Microbiota, Gastrointestinal Microbiome
- Abstract
The nature and composition of the waste produced by food industrial processing make its abundance and accumulation an environmental problem. Since these by-products may present a high potential for revalorization and may be used to obtain added-value compounds, the main goals of the technological advancements have been targeted at reducing the environmental impact and benefiting from the retrieval of active compounds with technological and health properties. Among the added-value substances, nondigestible carbohydrates have demonstrated promise. In addition to their well-known technological properties, they have been discovered to modify the gut microbiota and enhance immune function, including the stimulation of immune cells and the control of inflammatory reactions. Furthermore, the combination of these compounds with other substances such us phenols could improve their biological effect on different noncommunicable diseases through microbiota modulation. In order to gain insight into the implementation of this combined strategy, a broader focus concerning different aspects is needed. This review is focused on the optimized green and advanced extraction system applied to obtain added-value nondigestible carbohydrates, the combined administration with phenols and their beneficial effects on microbiota modulation intended for health and/or illness prevention, with particular emphasis on noncommunicable diseases. The isolation of nondigestible carbohydrates from by-products as well as in combination with other bioactive substances could provide an affordable and sustainable source of immunomodulatory chemicals.
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- 2023
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23. Ocular involvement in sphenopalatin ganglion pulsed radiofrequency.
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Temblador Barba I, Lozano Sánchez JJ, and Rodríguez Suárez AH
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- Humans, Treatment Outcome, Face, Eye, Pulsed Radiofrequency Treatment methods, Trigeminal Neuralgia therapy
- Abstract
Pulsed radiofrequency (PRF) treatment of the sphenopalatine ganglion is an important interventional treatment in refractory cases of trigeminal neuralgia (TN) or atypical facial pain, given the easy access to its location. Despite the fact that complications from this technique are rare and it is a fairly safe procedure, ophthalmologists should know about it due to the anatomical relations of this ganglion., (Copyright © 2023 Sociedad Española de Oftalmología. Published by Elsevier España, S.L.U. All rights reserved.)
- Published
- 2023
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24. Effect of micronization on olive pomace biotransformation in the static model of colonic fermentation.
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Monteiro CS, Bortolazzo PC, Bonini CAA, Dluzniewski LT, da Silva DT, Baranzelli J, Smaniotto FA, Ballus CA, Lozano-Sánchez J, Somacal S, and Emanuelli T
- Subjects
- Humans, Fermentation, Phenols analysis, Biotransformation, Olive Oil, Olea metabolism
- Abstract
The effect of granulometric fractionation and micronization of olive pomace (OP) on the biotransformation of phenolic compounds by intestinal microbiota was investigated in vitro. Three types of powdered OP samples were incubated with human feces to simulate colonic fermentation, after a sequential static digestion: non-fractionated OP (NF), granulometrically fractionated OP (GF) and granulometrically fractionated and micronized OP (GFM). GF and GFM favored the release of hydroxytyrosol, oleuropein aglycone, apigenin and phenolic acid metabolites in the first hours of colonic fermentation compared to NF (up to 41-fold higher). GFM caused higher release of hydroxytyrosol than GF. GFM was the only sample to release tyrosol and sustained tyrosol levels up to 24 h of fermentation. Micronization associated with granulometric fractionation was more efficient than granulometric fractionation alone to increase the release of phenolic compounds from the OP matrix during simulated colonic fermentation and can be further studied for nutraceutical purposes., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
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- 2023
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25. Bioactive Potential of Aqueous Phenolic Extracts of Spices for Their Use in the Food Industry-A Systematic Review.
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Duque-Soto C, Ruiz-Vargas A, Rueda-Robles A, Quirantes-Piné R, Borrás-Linares I, and Lozano-Sánchez J
- Abstract
The interest on the use of natural sources in the food industry has promoted the study of plants' phenolic compounds as potential additives. However, the literature has been focusing on essential oils, with very few studies published regarding aqueous extracts, their phenolic composition, and bioactivity. A systematic review was conducted on different databases following PRISMA guidelines to evaluate the relevance of the phenolic content of different aromatic spices (oregano, rosemary, thyme, ginger, clove, and pepper), as related to their bioactivity and potential application as food additives. Although different extraction methods have been applied in the literature, the use of green approaches using ethanol and deep eutectic solvents has increased, leading to the development of products more apt for human consumption. The studied plants present an interesting phenolic profile, ranging from phenolic acids to flavonoids, establishing a correlation between their phenolic content and bioactivity. In this sense, results have proven to be very promising, presenting those extracts as having similar if not higher bioactivity than synthetic additives already in use, with associated health concerns. Nevertheless, the study of spices' phenolic extracts is somehow limited to in vitro studies. Therefore, research in food matrices is needed for more understanding of factors interfering with their preservation activity.
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- 2023
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26. Effect of Microwave and Ultrasound-Assisted Extraction on the Phytochemical and In Vitro Biological Properties of Willow ( Salix alba) Bark Aqueous and Ethanolic Extracts.
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Aleman RS, Marcia J, Duque-Soto C, Lozano-Sánchez J, Montero-Fernández I, Ruano JA, Hoskin RT, and Moncada M
- Abstract
White willow ( Salix alba ) is a medicinal plant used in folk medicine. In this study, aqueous and ethanolic willow bark extracts were obtained via ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE), and analyzed regarding their phytochemical (total phenolics, phenolic acids, flavonoids, and tannins) content and in vitro biological properties (antibacterial and antifungal activity, acetylcholinesterase AChE inhibitory activity and anti-inflammatory effects). The highest phenolic, tannin, and flavonoid contents were found for willow bark extracts obtained via microwave-assisted extraction using ethanol as a solvent (SA-ME). The polyphenol load of all MAE and UAE extracts was higher when conventional solid-liquid extraction was applied ( ρ < 0.05). The antioxidant capacities were stronger for microwave-assisted ethanolic extracts, with the lowest IC
50 values of 12 μg/mL for DPPH• and a value of 16 μg/mL for ABTS•+, whereas the conventional extraction had the highest IC50 values (22 μg/mL and 28 μg/mL, respectively). Willow bark extract showed antibacterial activity against Gram-positive bacteria S. aureus and P. aeruginosa. AChE inhibitory activity was dependent on the extraction method and solvent used, and the highest inhibition among samples was observed for SA-ME. Taken altogether, our findings suggest that willow ( Salix alba) bark extract obtained via ethanolic microwave-assisted extraction is a phytochemical-rich resource with in vitro, anti-inflammatory, and AchE inhibitory properties and, therefore, potential multiple medicinal end-uses.- Published
- 2023
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27. Reduced-sodium roasted chicken: Physical/technological characteristics, optimized KCl-seasoning mixture, consumer perception, liking, emotions, and purchase intent.
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Aleman RS, Delarca Ruiz F, Pournaki SK, Marcia J, Montero I, Rueda-Robles A, Borrás-Linares I, and Lozano-Sánchez J
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- Animals, Chickens, Potassium Chloride, Emotions, Taste, Meat, Perception, Consumer Behavior, Sodium, Sodium Chloride
- Abstract
For thousands of years, sodium chloride (NaCl) has been used as a preservative and flavor enhancer. In the organism, NaCl plays a role in nerve functions, osmotic pressure, and nutrient absorption. However, high consumption of NaCl could lead to health issues, such as hypertension and heart-related problems. For these reasons, potassium chloride (KCl) has been considered a salt substitute in foods, but KCl could be limited to food matrixes because of its unwanted bitterness and metallic aftertaste. As a result, the objective of this study was to analyze KCl-reduced-sodium roasted chicken in physical/technological characteristics, KCl-seasoning mixture, consumer perception, liking, emotions, and purchase intent (PI). An extreme vertice mixture design decided granulated garlic (74.09%), black pepper (9.95%), smoked paprika (14.47%), and KCl (1.39%) ratio of good seasoning-KCl mixture for roasted chicken based on sensory attributes used on the desirability function methodology. After optimizing the KCl-seasoning blend, NaCl/KCl replacement levels (0%, 25%, 50%, 75%, and 100%) were established and evaluated consumer perception, liking, emotions, and PI. Adding 25% and 50% of KCl showed no significant (p > 0.05) impact on the sensory attributes. Likewise, PI significantly (p < 0.05) increased when utilizing 25% and 50% of KCl after panelists received information about sodium health risks (SHR). Regarding emotional responses, unsafe and worried significantly (p < 0.05) decreased among the highest KCl replacement levels (75% and 100%) after panelists obtained the SHR. Overall liking, gender, age, salt user, and positive emotional responses (satisfied and pleased) were decisive predictors concerning PI among panelists., (© 2023 Institute of Food Technologists.)
- Published
- 2023
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28. Co-encapsulation of Lactobacillus plantarum and bioactive compounds extracted from red beet stem (Beta vulgaris L.) by spray dryer.
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de Deus C, Eduardo de Souza Brener C, Marques da Silva T, Somacal S, Queiroz Zepka L, Jacob Lopes E, de Bona da Silva C, Teixeira Barcia M, Lozano Sánchez J, and Ragagnin de Menezes C
- Subjects
- Microbial Viability, Preservation, Biological, Beta vulgaris, Lactobacillus plantarum, Probiotics
- Abstract
Probiotic bacteria and bioactive compounds obtained from plant origin stand out as ingredients with the potential to increase the healthiness of functional foods, as there is currently a recurrent search for them. Probiotics and bioactive compounds are sensitive to intrinsic and extrinsic factors in the processing and packaging of the finished product. In this sense, the present study aims to evaluate the co-encapsulation by spray dryer (inlet air temperature 120 °C, air flow 40 L / min, pressure of 0.6 MPa and 1.5 mm nozzle diameter) of probiotic bacteria (L.plantarum) and compounds extracted from red beet stems (betalains) in order to verify the interaction between both and achieve better viability and resistance of the encapsulated material. When studying the co-encapsulation of L.plantarum and betalains extracted from beet stems, an unexpected influence was observed with a decrease in probiotic viability in the highest concentration of extract (100 %), on the other hand, the concentration of 50 % was the best enabled and maintained the survival of L.plantarum in conditions of 25 °C (63.06 %), 8 °C (88.80 %) and -18 °C (89.28 %). The viability of the betalains and the probiotic was better preserved in storage at 8 and -18 °C, where the encapsulated stability for 120 days was successfully achieved. Thus, the polyfunctional formulation developed in this study proved to be promising, as it expands the possibilities of application and development of new foods., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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29. Extraction, Characterization, and Bioactivity of Phenolic Compounds-A Case on Hibiscus Genera.
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Duque-Soto C, Expósito-Almellón X, García P, Pando ME, Borrás-Linares I, and Lozano-Sánchez J
- Abstract
Phenolic compounds have recently gained interest, as they have been related to improvements in health and disease prevention, such as inflammatory intestinal pathologies and obesity. However, their bioactivity may be limited by their instability or low concentration in food matrices and along the gastrointestinal tract once consumed. This has led to the study of technological processing with the aim of optimizing phenolic compounds' biological properties. In this sense, different extraction systems have been applied to vegetable sources for the purpose of obtaining enriched phenolic extracts such as PLE, MAE, SFE, and UAE. In addition, many in vitro and in vivo studies evaluating the potential mechanisms of these compounds have also been published. This review includes a case study of the Hibiscus genera as an interesting source of phenolic compounds. The main goal of this work is to describe: (a) phenolic compound extraction by designs of experiments (DoEs) applied to conventional and advanced systems; (b) the influence of the extraction system on the phenolic composition and, consequently, on the bioactive properties of these extracts; and (c) bioaccessibility and bioactivity evaluation of Hibiscus phenolic extracts. The results have pointed out that the most used DoEs were based on response surface methodologies (RSM), mainly the Box-Behnken design (BBD) and central composite design (CCD). The chemical composition of the optimized enriched extracts showed an abundance of flavonoids, as well as anthocyanins and phenolic acids. In vitro and in vivo studies have highlighted their potent bioactivity, with particular emphasis on obesity and related disorders. This scientific evidence establishes the Hibiscus genera as an interesting source of phytochemicals with demonstrated bioactive potential for the development of functional foods. Nevertheless, future investigations are needed to evaluate the recovery of the phenolic compounds of the Hibiscus genera with remarkable bioaccessibility and bioactivity.
- Published
- 2023
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30. Evaluation of Different Advanced Approaches to Simulation of Dynamic In Vitro Digestion of Polyphenols from Different Food Matrices-A Systematic Review.
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Duque-Soto C, Quintriqueo-Cid A, Rueda-Robles A, Robert P, Borrás-Linares I, and Lozano-Sánchez J
- Abstract
Phenolic compounds have become interesting bioactive antioxidant compounds with implications for obesity, cancer and inflammatory gastrointestinal pathologies. As the influence of digestion and gut microbiota on antioxidant behavior is yet to be completely elucidated, and due to limitations associated to in vivo studies, dynamic in vitro gastrointestinal models have been promoted. A systematic review was conducted of different databases (PubMed, Web of Science and Scopus) following PRISMA guidelines to assess different dynamic digestion models and assay protocols used for phenolic compound research regarding bioaccesibility and interaction with colonic microbiota. Of 284 records identified, those including dynamic multicompartmental digestion models for the study of phenolic compound bioaccesibility, bioactivity and the effects of microbiota were included, with 57 studies meeting the inclusion criteria. Different conditions and experimental configurations as well as administered doses, sample treatments and microbiological assays of dynamic digestion studies on polyphenols were recorded and compared to establish their relevance for the dynamic in vitro digestion of phenolic compounds. While similarities were observed in certain experimental areas, a high variability was found in others, such as administered doses. A description of considerations on the study of the digestion of phenolic compounds is proposed to enhance comparability in research.
- Published
- 2022
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31. Analysis and Screening of Commercialized Protein Supplements for Sports Practice.
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Rodriguez-Lopez P, Rueda-Robles A, Sánchez-Rodríguez L, Blanca-Herrera RM, Quirantes-Piné RM, Borrás-Linares I, Segura-Carretero A, and Lozano-Sánchez J
- Abstract
Recent years have seen a rise in the popularity of the consumption of sports-related supplements. However, the hypothesis is raised that it is necessary to analyze the quality aspects of these supplements in relation to the information provided on the label, to avoid associated risks and obtain the greatest possible benefit from their consumption. Therefore, the aim of this study has been to carry out an analysis or screening of the protein supplements that are currently marketed in Spain. We analyzed the labels of 52 protein sports supplements available both in physical stores and online. The analysis consisted of addressing three relevant aspects considering the labeling: (a) the legislative framework in which the supplements are marketed, (b) the quality of the protein, and (c) the presence of other ingredients according to the specifications of the label. In the legislative context, there do not seem to be any specific regulations to guarantee consumer protection, which can lead to unfair practices and misleading advertising. Most of the supplements analyzed to comply with the requirements of their current regulations. However, claims about their benefits that are not allowed under European legislation have been found in some of them. Regarding composition and according to label information, the supplements have been found to provide a sufficient dose of protein in terms of recommended protein intake per serving. Regarding the presence of other ingredients and according to the information on the label, most of them, except for egg supplements, contain other ingredients. Colostrum was also found in one of the supplements evaluated. The conclusions of the study reveal that, due to a lack of knowledge or misleading advertising practices, supplements are often not used properly. The information provided is essential for both professionals and consumers to avoid the risks associated with consumption, such as unintentional doping, interactions between ingredients that reduce the quality of the supplement, and consumption of supplements inappropriately, among others.
- Published
- 2022
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32. Use of red onion (Allium cepa L.) residue extract in the co-microencapsulation of probiotics added to a vegan product.
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Carine Raddatz G, Sonza Pinto V, Queiroz Zepka L, Smanioto Barin J, José Cichoski A, de Bona da Silva C, Lozano-Sánchez J, Gomes da Cruz A, and Ragagnin de Menezes C
- Subjects
- Alginates chemistry, Anthocyanins, Humans, Plant Extracts, Vegans, Onions, Probiotics chemistry
- Abstract
This study aimed to develop a functional strawberry pulp containing the combination of Lactobacillus casei and bioactive compounds from red onion peel extract into the microparticles formulations to improve bacteria survival during storage and product consumption. To achieve this goal, the addition of different concentrations of red onion peel extract added to the microparticles was evaluated: 5, 20 and 40 %. Microparticles were morphologically characterized and the encapsulation efficiency of the bioactive compounds were evaluated. The physicochemical and microbiological characteristics of the fruit pulp were within the required standards, regardless of the formulation evaluated. As for the pulp added from the microparticles, their physicochemical and microbiological features and probiotic survival under simulated gastrointestinal conditions and storage were analyzed; the size of the microparticles ranged from 136.00 to 305.00 µm. The encapsulation efficiency of both, probiotics and compounds was satisfactory over the different treatments. Indeed, the results pointed out values in the range from 77.77 to 92.11 % for probiotic bacteria; from28.88 to 50.18 % for reducing compounds; 35.72 to 69.01 % for flavonoids; and 25.39 to 60.00 % for total monomeric anthocyanins. The formulations of alginate microparticles and alginate +5 % extract had the best results of L. casei probiotic viability in strawberry pulp under simulated gastrointestinal conditions and during storage at -18 °C for 60 days. In conclusion, red onion peel extract at low concentrations can help the survival of the probiotic L. casei under different conditions., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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33. Enhancing the Production of the Phenolic Extracts of Asparagus Using an Advanced Green Process.
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López-Salas L, Borrás-Linares I, Quirantes-Piné R, Emanuelli T, Segura-Carretero A, and Lozano-Sánchez J
- Abstract
Asparagus officinalis L. is a common vegetable widely consumed due to its high consumer acceptance. In addition to its flavor, green asparagus contains a high amount of bioactive compounds with health-promoting effects. In this sense, the growing concern of the public health system to promote a diet with a higher consumption of vegetables makes research on phytochemicals from this food of interest. In order to study the content of bioactive compounds from plant matrices, the combination of advanced extraction and analytical techniques within the context of green chemistry is an indispensable working model in today's research. In the present experimental work, the composition of the phytochemicals of green asparagus from the Protected Geographical Indication (PGI) located in Huétor Tájar, Granada (Spain), was evaluated by environmentally friendly extraction techniques. In order to carry out this work, the recovery of bioactive compounds was evaluated by pressurized liquid extraction (PLE) using GRAS (Generally Recognized As Safe) solvents (mixtures of water and ethanol). The extraction was optimized using a Response Surface Methodology (RSM) based on a 2
4 factorial Central Composite Design (CCD). The experimental model was followed by high-performance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOF-MS) analytical methodology for a comprehensive characterization. The optimized methodology was compared with conventional solid-liquid extraction protocols using ethanol and water. The results highlighted the potential of advanced PLE techniques compared to conventional systems for the recovery of green asparagus phytochemicals. Moreover, the analytical characterization allowed the identification and quantitation of major phenolic compounds belonging to phenolic acids and flavonoids families. Therefore, an easy, fast, and novel methodology to optimize the extraction of bioactive compounds from green asparagus has been optimized, using Green and GRAS methodology, which enables a better understanding of the bioactive composition of this widely consumed food.- Published
- 2022
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34. Application of Response Surface Methodologies to Optimize High-Added Value Products Developments: Cosmetic Formulations as an Example.
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Leyva-Jiménez FJ, Fernández-Ochoa Á, Cádiz-Gurrea ML, Lozano-Sánchez J, Oliver-Simancas R, Alañón ME, Castangia I, Segura-Carretero A, and Arráez-Román D
- Abstract
In recent years, green and advanced extraction technologies have gained great interest to revalue several food by-products. This by-product revaluation is currently allowing the development of high value-added products, such as functional foods, nutraceuticals, or cosmeceuticals. Among the high valued-added products, cosmeceuticals are innovative cosmetic formulations which have incorporated bioactive natural ingredients providing multiple benefits on skin health. In this context, the extraction techniques are an important step during the elaboration of cosmetic ingredients since they represent the beginning of the formulation process and have a great influence on the quality of the final product. Indeed, these technologies are claimed as efficient methods to retrieve bioactive compounds from natural sources in terms of resource utilization, environmental impact, and costs. This review offers a summary of the most-used green and advanced methodologies to obtain cosmetic ingredients with the maximum performance of these extraction techniques. Response surface methodologies may be applied to enhance the optimization processes, providing a simple way to understand the extraction process as well as to reach the optimum conditions to increase the extraction efficiency. The combination of both assumes an economic improvement to attain high value products that may be applied to develop functional ingredients for cosmetics purposes.
- Published
- 2022
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35. Potential Antioxidant and Antiviral Activities of Hydroethanolic Extracts of Selected Lamiaceae Species.
- Author
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Duque-Soto C, Borrás-Linares I, Quirantes-Piné R, Falcó I, Sánchez G, Segura-Carretero A, and Lozano-Sánchez J
- Abstract
Medicinal and aromatic plants (MAPs) are potential sources of natural bioactive phytochemical compounds of an incredible worth for the food industry, such as polyphenols. Lamiaceae medicinal and aromatic plants from Granada's high plateau, concretely Origanum bastetanum , Thymus zygis gracilis , Thymus longiflorus , Thymus membranaceus and Ziziphora hispanica , were evaluated under different conventional solid-liquid extraction conditions to obtain extracts enriched in bioactive compounds. Phenolic profile was detected by HPLC-QTOF-MS, identifying a high abundance of bioactive constituents. Furthermore, antioxidant and antiviral activities of the mentioned plants were studied as biological properties of interest for the improvement of food shelf-life. Thus, Origanum bastetanum showed the highest antioxidant potential for all assays. Antiviral activity was also tested against some important foodborne viruses, feline calicivirus (FCV), murine norovirus (MNV) and hepatitis A virus (HAV), with the highest activity obtained for Ziziphora hispanica , Thymus longiflorus and Origanum bastetanum . This research proposes the studied plants as rich sources of bioactive compounds with potential use as preservatives in the food industry.
- Published
- 2022
- Full Text
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36. Characterization and Influence of Static In Vitro Digestion on Bioaccessibility of Bioactive Polyphenols from an Olive Leaf Extract.
- Author
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Duque-Soto C, Quirantes-Piné R, Borrás-Linares I, Segura-Carretero A, and Lozano-Sánchez J
- Abstract
Olive leaves, one of the most abundant olive production by-products, have shown incredible potential for their characteristic bioactive compound composition, with unique compounds such as the polyphenol oleuropein. In order to evaluate the bioaccessibility of bioactive compounds present in an olive leaf extract, samples were submitted to an in vitro digestion process following INFOGEST protocol, and qualitative and quantitative characterization of the original extract and digestive samples at different times were carried out using HPLC-ESI-TOF-MS. The analyzed extract presented an abundance of phenolic compounds, such as secoiridoids, with oleuropein being the main identified compound. The in vitro digestion process showed an effect on the phenolic profile of the extract, with a lower recovery in the gastric phase and an increase at the beginning of the intestinal phase. Most of the studied compounds showed high bioaccessibility at the end of the digestion, with oleuropein, ligstroside, and quercetin-3- O -galactoside being among the ones with higher value. These findings show the potential for future use of olive leaf polyphenols. However, further research is needed in order to evaluate the absorption, delivery, and interaction of these compounds with the colon.
- Published
- 2022
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37. RP-HPLC-ESI-QTOF-MS Qualitative Profiling, Antioxidant, Anti-Enzymatic, Anti-Inflammatory, and Non-Cytotoxic Properties of Ephedra alata Monjauzeana.
- Author
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Khattabi L, Boudiar T, Bouhenna MM, Chettoum A, Chebrouk F, Chader H, Lozano-Sánchez J, Segura-Carretero A, Nieto G, and Akkal S
- Abstract
An investigation was conducted to study the beneficial effects of Ephedra alata monjauzeana crude extract (EamCE). The chemical profile was determined using RP-HPLC-ESI-QTOF-MS analysis, revealing the presence of twenty-one flavonoids and phenolic acids. A series of antioxidant assays was carried out using ten different methods. The EamCE has demonstrated a significant antioxidant potential, with interesting IC
50 values not exceeding 40 µg/mL in almost activities. Likewise, a significant inhibition of key enzymes, involved in some health issues, such as Alzheimer's disease, diabetes, hyperpigmentation, dermatological disorders, gastric/urinary bacterial infections, and obesity, was observed for the first time. The IC50 values ranged from 22.46 to 54.93. The anti-inflammatory and non-cytotoxic activities were assessed by heat-induced hemolysis and cell culture methods, respectively; the EamCE has shown a prominent effect in both tests, notably for the anti-inflammatory effect that was superior to the reference compound "diclofenac" (IC50 : 71.03 ± 1.38 > 70.23 ± 0.99 (µg/mL)). According to these results, this plant could be used in a large spectrum as a food supplement, as a natural remedy for various physiological disorders and pathologies; and it might serve as a preventive and health care agent.- Published
- 2022
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38. Extraction of the antioxidant phytocomplex from wine-making by-products and sustainable loading in phospholipid vesicles specifically tailored for skin protection.
- Author
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Perra M, Lozano-Sánchez J, Leyva-Jiménez FJ, Segura-Carretero A, Pedraz JL, Bacchetta G, Muntoni A, De Gioannis G, Manca ML, and Manconi M
- Subjects
- 3T3 Cells, Animals, Antioxidants administration & dosage, Antioxidants isolation & purification, Fibroblasts drug effects, Fibroblasts pathology, Hydrogen Peroxide, Liposomes, Mice, Oxidative Stress drug effects, Particle Size, Phospholipids chemistry, Phytochemicals administration & dosage, Phytochemicals isolation & purification, Phytochemicals pharmacology, Plant Extracts administration & dosage, Plant Extracts chemistry, Skin pathology, Wine analysis, Antioxidants pharmacology, Plant Extracts pharmacology, Skin drug effects, Vitis chemistry
- Abstract
The present study is aimed at valorizing grape pomace, one of the most abundant winery-making by-products of the Mediterranean area, through the extraction of the main bioactive compounds from the skin of grape pomace and using them to manufacture innovative nanoformulations capable of both avoiding skin damages and promoting skincare. The phytochemicals were recovered through maceration in hydroethanolic solution. Catechin, quercetin, fisetin and gallic acid, which are known for their antioxidant power, were detected as the main compounds of the extract. Liposomes and phospholipid vesicles modified with glycerol or Montanov 82® or a combination of both, were used as carriers for the extract. The vesicles were small (~183 nm), slightly polydispersed (PI ≥ 0.28), and highly negatively charged (~-50 mV). The extract was loaded in high amounts in all vesicles (~100%) irrespective of their composition. The antioxidant activity of the extract, measured by using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, was 84 ± 1%, and slightly increased when loaded into the vesicles (~89%, P < 0.05). The grape pomace extract loaded vesicles were highly biocompatible and able to protect fibroblasts (3T3) from the oxidative stress induced by hydrogen peroxide., (Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Published
- 2021
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39. Functional ingredient from avocado peel: Microwave-assisted extraction, characterization and potential applications for the food industry.
- Author
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Figueroa JG, Borrás-Linares I, Del Pino-García R, Curiel JA, Lozano-Sánchez J, and Segura-Carretero A
- Subjects
- Anti-Bacterial Agents pharmacology, Antioxidants pharmacology, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Plant Extracts pharmacology, Anti-Bacterial Agents isolation & purification, Antioxidants isolation & purification, Food Industry, Microwaves, Persea chemistry, Plant Extracts isolation & purification
- Abstract
Avocado peel is a by-product obtained in high amounts in the food industry with no further applications despite its richness in bioactive compounds. In this context, an efficient "green" microwave assisted extraction (MAE) was optimized to maximize the extraction of bioactive polyphenols. Moreover, the phenolic composition of the developed green avocado extract was characterized by HPLC coupled to MS analysers and the potential applications for the food industry were studied assaying different bioactivities. Thus, the matrix metalloproteinases inhibition, the antioxidant capacity and the antimicrobial activity against gram-positive and gram-negative bacteria, yeast and mold were tested. The results pointed out both, high matrix metalloproteinases inhibitory capacity and antioxidant activity of avocado peel MAE extract. These findings suggest the potential food industry applications of this extract as natural food preservative, functional food ingredient or nutraceuticals with antioxidant and anti-aging activities., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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40. Preliminary Investigation of Different Drying Systems to Preserve Hydroxytyrosol and Its Derivatives in Olive Oil Filter Cake Pressurized Liquid Extracts.
- Author
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López-Salas L, Cea I, Borrás-Linares I, Emanuelli T, Robert P, Segura-Carretero A, and Lozano-Sánchez J
- Abstract
Phenolic compounds present in extra virgin olive oil (EVOO) could be retained in its byproducts during processing. Among them, hydroxytyrosol and its derivatives deserve special attention due to their health benefits recognized by The European Food Safety Authority (EFSA). In the present research, the presence of these compounds in the filter cake byproduct was studied by combining pressurized liquid extraction (PLE) and high-performance liquid chromatography coupled to time-of-flight mass spectrometry (HPLC-TOF-MS). The applied optimum extraction parameters were 1500 psi, 120 °C and aqueous ethanol (50:50, v / v). The influence of different drying methods (vacuum-, freeze- and spray-drying) in the recovery of phenolic compounds was also evaluated. A total of 16 compounds from EVOO were identified in the extracts, 3 of them being hydroxytyrosol-related compounds, 6 substances of oleoside and elenolic acid derivatives, together with 6 secoiridoids and 1 lignan. The results highlighted the great number of phenolic compounds recovered from filter cake with these techniques, being even higher than the reported content in EVOO and other byproducts. The combination of PLE and freeze-drying resulted in being the best procedure for the recovery of phenolic compounds from filter cake byproduct.
- Published
- 2021
- Full Text
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41. Micronization increases the bioaccessibility of polyphenols from granulometrically separated olive pomace fractions.
- Author
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Sefrin Speroni C, Rigo Guerra D, Beutinger Bender AB, Stiebe J, Ballus CA, Picolli da Silva L, Lozano-Sánchez J, and Emanuelli T
- Subjects
- Antioxidants chemistry, Caffeic Acids analysis, Chromatography, High Pressure Liquid, Dietary Supplements analysis, Digestion, Iridoid Glucosides, Iridoids analysis, Olea metabolism, Olive Oil chemistry, Plant Extracts chemistry, Principal Component Analysis, Tandem Mass Spectrometry, Food Handling methods, Olea chemistry, Polyphenols analysis
- Abstract
The effect of micronization of granulometrically fractionated olive pomace (OP) on the bioaccessibility of polyphenols and the antioxidant capacity was investigated during sequential in vitro static digestion. Crude OP was fractionated in a 2-mm sieve (F1: > 2 mm; F2: < 2 mm) and then micronized (300 r min
-1 , 5 h) generating F1AG (17.8 μm) and F2AG (15.6 μm). Micronization increased the release of hydroxytyrosol, oleuropein, caffeic acid, and decarboxymethyl oleuropein aglycone (3,4-DHPEA-EDA) in the salivary and gastric phase, beyond luteolin in the gastric phase. Micronization also increased the intestinal bioaccessibility of hydroxytyrosol, 3,4-DHPEA-EDA, oleuropein, luteolin, and apigenin; it was more effective for F2AG than F1AG. Micronized samples increased antioxidant capacity in the gastric phase. F2AG exhibited the highest antioxidant capacity in the insoluble intestinal fraction. Thus, micronization can be further exploited to improve the nutraceutical properties of OP by increasing the bioaccessibility and antioxidant capacity of phenolic compounds., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2021
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42. Antioxidant activity and characterization of flavonoids and phenolic acids of Ammoides atlantica by RP-UHPLC-ESI-QTOF-MS n .
- Author
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Benteldjoune M, Boudiar T, Bakhouche A, Del Mar Contreras M, Lozano-Sánchez J, Bensouici C, Kabouche Z, and Segura-Carretero A
- Subjects
- Antioxidants chemistry, Chromatography, High Pressure Liquid, Coumaric Acids chemistry, Coumaric Acids pharmacology, Flavonoids chemistry, Hydroxybenzoates chemistry, Phenols analysis, Phytochemicals chemistry, Phytochemicals pharmacology, Plant Extracts chemistry, Tandem Mass Spectrometry, Antioxidants pharmacology, Apiaceae chemistry, Flavonoids pharmacology, Hydroxybenzoates pharmacology, Spectrometry, Mass, Electrospray Ionization
- Abstract
In this study, phytochemical profiling of hydro-alcoholic extract of Ammoides atlantica aerial parts has been carried out using RP-UHPLC-ESI-QTOF-MS in negative ionization mode Chemical characterization was established according to the MS and MS/MS spectra. A total of 66 chemical compounds were detected. Among these, 45 compounds were identified: hydroxycinnamic acid and derivatives (26), hydroxybenzoic acids (4), flavones (11), flavonols (3), and a lignan. Total phenolics (371.57 mg/g) and total flavonoids (41.02 mg/g) contents were also determined. Moreover, the antioxidant activity of A. atlantica extract was also studied by six methods: DPPH (IC
50 : 23.31 µg/mL), ABTS+ (IC50 : 11.31 µg/mL), O2 - DMSO alkaline (IC50 : 3.19 µg/mL), ferrous ions chelating assays (IC50 : 102.35 µg/mL), reducing power (A0.50 : 92.70 µg/mL) and CUPRAC (A0.50 : 13.56 µg/mL) assays. These results suggest that the antioxidant activity of the hydroalcoholic extract was comparable to common antioxidant additives in most of the tests, representing a good alternative.- Published
- 2021
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43. Olive oil varieties and ripening stages containing the antioxidants hydroxytyrosol and derivatives in compliance with EFSA health claim.
- Author
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López-Huertas E, Lozano-Sánchez J, and Segura-Carretero A
- Subjects
- Food Safety, Phenylethyl Alcohol analysis, Antioxidants analysis, Insurance Benefits, Olive Oil chemistry, Phenylethyl Alcohol analogs & derivatives
- Abstract
Virgin olive oils (VOO) and extra virgin olive oils (EVOO) contain a specific fraction of polyphenols (hydroxytyrosol and its derivatives) that produce beneficial physiological effects. The European Food Safety Authority (EFSA) authorised a health claim for olive oil (OO) containing at least 250 mg/Kg of those polyphenols. The specific polyphenol content of twelve varieties of EVOO extracted at three different maturation stages was investigated. The total concentration of specific polyphenols changed depending on the olive oil variety. The varieties showing the highest specific polyphenol content (all above 250 mg/Kg) were Lechín Sevilla (429.5 ± 5), Manzanilla Sevilla (407.6 ± 6) and Cornezuelo (394.0 ± 6) in the green phase; Cornicabra (362.0 ± 8), Nevadillo Negro (326.5 ± 4) and Picual 296.0 ± 6) in the turning phase; and Lechin Granada (382.8 ± 4), Picual (317.7 ± 3), Lechin Sevilla (294.4 ± 5) and Manzanilla Sevilla (278.0 ± 2) in the mature phase. These results could have potential application for the industrial production of a category of healthy antioxidant OO., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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44. Identification of Bioactive Compounds of Asparagus officinalis L.: Permutation Test Allows Differentiation among "Triguero" and Hybrid Green Varieties.
- Author
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Jiménez-Sánchez C, Pedregosa F, Borrás-Linares I, Lozano-Sánchez J, and Segura-Carretero A
- Subjects
- Chromatography, High Pressure Liquid methods, Flavonoids chemistry, Flavonols chemistry, Saponins chemistry, Spectrometry, Mass, Electrospray Ionization methods, Asparagus Plant chemistry, Phytochemicals chemistry, Plant Extracts chemistry
- Abstract
In this study, we determined the phytochemical profile of the Spanish "triguero" asparagus landrace "verde-morado" ( Asparagus officinalis L.), a wild traditional landrace, and the improved "triguero" HT-801, together with two commercial green asparagus varieties. For comparison, we used reverse-phase high-performance liquid chromatography coupled with diode array electrospray time-of-flight mass spectrometry (RP-HPLC-DAD-ESI-TOF/MS) followed by a permutation test applied using a resampling methodology valid under a relaxed set of assumptions, such as i.i.d. errors (not necessarily normal) that are exchangeable under the null hypothesis. As a result, we postulate that "triguero" varieties (the improved HT-801 followed by its parent "verde-morado") have a significantly different phytochemical profile from that of the other two commercial hybrid green varieties. In particular, we found compounds specific to the "triguero" varieties, such as feruloylhexosylhexose isomers, or isorhamnetin-3- O -glucoside, which was found only in the "triguero" variety HT-801. Although studies relating the phytochemical content of "triguero" asparagus varieties to its health-promoting effects are required, this characteristic phytochemical profile can be used for differentiating and revalorizating these asparagus cultivars.
- Published
- 2021
- Full Text
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45. Development of an Innovative Pressurized Liquid Extraction Procedure by Response Surface Methodology to Recover Bioactive Compounds from Carao Tree Seeds.
- Author
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Fuentes JAM, López-Salas L, Borrás-Linares I, Navarro-Alarcón M, Segura-Carretero A, and Lozano-Sánchez J
- Abstract
Nowadays there are evidences from several studies which have revealed the protective effects of food against chronic diseases. These healthy properties have been related to bioactive compounds. Among bioactive substances, the scientific interest in phenolic compounds has stimulated multidisciplinary research on the composition of plant phenolic compounds. The aim of this work has been to determine the bioactive composition of Carao tree seeds ( Cassia grandis ) and to optimize the recovering of these compounds for developing functional ingredients. To achieve this goal, pressurized liquid extraction (PLE) has been applied to recover these phytochemicals. The optimization of this innovative extraction procedure was performed by a response surface methodology (RSM) based on a central composite design 2
3 model to address the bioactive compounds extraction. Phenolic compounds recovered by PLE were characterized using reversed-phase high-performance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOF-MS). Analytical characterization allowed the identification and quantitation of phenolic compounds belonging to hydroxybenzoic acids and flavonoids (flavonols, flavanols, flavanones and proanthocyanidins). Phytochemical concentrations were used as response variable in order to get the best extraction conditions. These results pointed out that Carao tree seeds can be a potential source of bioactive compounds and PLE extracts could be used as functional ingredients.- Published
- 2021
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46. Development of advanced phospholipid vesicles loaded with Lippia citriodora pressurized liquid extract for the treatment of gastrointestinal disorders.
- Author
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Leyva-Jiménez FJ, Manca ML, Manconi M, Caddeo C, Vázquez JA, Carbone C, Lozano-Sánchez J, Arráez-Román D, and Segura-Carretero A
- Subjects
- Caco-2 Cells, Drug Compounding, Gastrointestinal Diseases metabolism, Humans, Liposomes, Oxidative Stress drug effects, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Plant Leaves chemistry, Gastrointestinal Diseases drug therapy, Lippia chemistry, Phospholipids chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Pressure
- Abstract
Pressurized liquid extraction was performed to obtain a phytocomplex from Lippia citriodora leaves rich in bioactive compounds. The extract was loaded in phospholipid vesicles to improve its protective effect against oxidative stress in the intestine. The phytochemicals were identified and quantified by HPLC-ESI-TOF-MS. The extract was incorporated in liposomes and penetration enhancer-containing vesicles (PEVs) modified with glucidex, a dextrin, and a biopolymer obtained from Chimaera monstrosa. The PEVs were smaller than liposomes (~150 vs 370 nm) and more stable, according to accelerated aging tests. The integrity of the vesicles in acidic or neutral pH and high ionic strength or in milk whey was assessed. The cytocompatibility of the formulations and their ability to protect Caco-2 cells against oxidative stress were confirmed in vitro and compared with two commercial extracts of L. citriodora. The results confirmed the suitability of formulations to be used in functional foods to protect the intestine from oxidative stress., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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47. Recovery of Bioactive Compounds from Pomegranate ( Punica granatum L.) Peel Using Pressurized Liquid Extraction.
- Author
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García P, Fredes C, Cea I, Lozano-Sánchez J, Leyva-Jiménez FJ, Robert P, Vergara C, and Jimenez P
- Abstract
Pressurized liquid extraction (PLE) is a clean and environmentally friendly alternative for the recovery of bioactive compounds from fruit by-products. Herein we focused on PLE for the extraction of bioactive compounds from pomegranate peel using a combination of pressurized water and ethanol. The main aim was to determine the optimal PLE conditions, i.e., ethanol percentage and process temperature, to obtain a pomegranate peel extract (PPE) with maximum total phenolic content (TPC), punicalagin content, and antimicrobial activity (AMA). The experimental design was conducted using a central composite design with axial points. Response surface methodology was applied to optimize the response variables using the desirability function. Multiple response optimization indicated a process temperature of 200 °C and ethanol of 77% as optimal conditions. The TPC and the punicalagin content of PPE-PLE obtained under optimal conditions were 164.3 ± 10.7 mg GAE/g DW and 17 ± 3.6 mg/g DW, respectively. Our findings support the efficacy of PLE on TPC recovery but not in punicalagin recovery. The AMA against S. aureus was 14 mm. The efficacy of PPE-PLE in food applications must continue to be studied in order to achieve adequate information on its potential for developing new food additives.
- Published
- 2021
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48. Revalorization of Broccoli By-Products for Cosmetic Uses Using Supercritical Fluid Extraction.
- Author
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Borja-Martínez M, Lozano-Sánchez J, Borrás-Linares I, Pedreño MA, and Sabater-Jara AB
- Abstract
The agri-food industry is currently one of the main engines of economic development worldwide. The region of Murcia is a reference area in Europe for the cultivation of fruits and vegetables and produces the bulk of Spanish exports of broccoli ( Brassica oleracea var. italica). The processing of fresh produce generates a huge number of by-products that represent an important economic and environmental problem when discarded. In this work, an advanced extraction technique using environmentally friendly solvents was applied to assess the revalorization of broccoli by-products, by performing a comparative analysis with conventional extraction. To achieve this goal, supercritical fluid extraction based on response surface methodology was performed using CO
2 and ethanol as solvents. The results obtained showed that the supercritical fluid extracts were rich in β-carotene, phenolic compounds, chlorophylls and phytosterols. Moreover, in bioactivity assays, the supercritical fluid extracts exhibited a high antioxidant activity and a cytoprotective effect in a non-tumorigenic keratinocyte cell line exposed to ultraviolet B light. The results indicate that supercritical fluid extracts from broccoli by-products could potentially serve as an ingredient for cosmetic purposes.- Published
- 2020
- Full Text
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49. Mimetics of extra virgin olive oil phenols with anti-cancer stem cell activity.
- Author
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Cuyàs E, Gumuzio J, Lozano-Sánchez J, Segura-Carretero A, Verdura S, Bosch-Barrera J, Martin-Castillo B, Nonell-Canals A, Llebaria A, Cabello S, Serra C, Sanchez-Martinez M, Martin ÁG, and Menendez JA
- Subjects
- Breast Neoplasms drug therapy, Cell Line, Tumor, DNA (Cytosine-5-)-Methyltransferases antagonists & inhibitors, DNA Methyltransferase 3A, Drug Discovery, Humans, TOR Serine-Threonine Kinases antagonists & inhibitors, Antineoplastic Agents administration & dosage, Antineoplastic Agents chemical synthesis, Biomimetic Materials administration & dosage, Biomimetic Materials chemical synthesis, Neoplastic Stem Cells drug effects, Olive Oil chemistry, Phenols chemistry
- Abstract
The extra virgin olive oil (EVOO) dihydroxy-phenol oleacein is a natural inhibitor of multiple metabolic and epigenetic enzymes capable of suppressing the functional traits of cancer stem cells (CSC). Here, we used a natural product-inspired drug discovery approach to identify new compounds that phenotypically mimic the anti-CSC activity of oleacein. We coupled 3D quantitative structure-activity relationship-based virtual profiling with phenotypic analysis using 3D tumorsphere formation as a gold standard for assessing the presence of CSC. Among the top 20 computationally-predicted oleacein mimetics, four fulfilled the phenotypic endpoint of specifically suppressing the tumorsphere-initiating capacity of CSC, in the absence of significant cytotoxicity against differentiated cancer cells growing in 2D cultures in the same low micromolar concentration range. Of these, 3,4-dihydrophenetyl butyrate -a lipophilic ester conjugate of the hydroxytyrosol moiety of oleacein- and (E)-N -allyl-2-((5-nitrofuran-2-yl)methylene)hydrazinecarbothioamide) -an inhibitor of Trypanosoma cruzi triosephosphate isomerase- were also highly effective at significantly reducing the proportion of aldehyde dehydrogenase (ALDH)-positive CSC-like proliferating cells. Preservation of the mTOR/DNMT binding mode of oleacein was dispensable for suppression of the ALDH
+ -CSC functional phenotype in hydroxytyrosol-unrelated mimetics. The anti-CSC chemistry of complex EVOO phenols such as oleacein can be phenocopied through the use of mimetics capturing its physico-chemical properties.- Published
- 2020
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- View/download PDF
50. Spray-Drying Microencapsulation of Bioactive Compounds from Lemon Verbena Green Extract.
- Author
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Leyva-Jiménez FJ, Lozano-Sánchez J, Cádiz-Gurrea ML, Fernández-Ochoa Á, Arráez-Román D, and Segura-Carretero A
- Abstract
Lippia citriodora has been demonstrated to have a wide variety of phytochemicals which provide benefits to human health acting as antioxidants or anti-obesogenics. In this study, these phytochemicals were recovered using a microwave-assisted technology and applying optimal conditions and microencapsulated using spray drying. In this study, two different carbohydrates, maltodextrin (MD) and inulin (IN), were compared as carriers in the encapsulation procedure. The spray drying process was optimized by using a response surface methodology (RSM) based on a central composite design 2
2 , where air inlet temperature and the sample:encapsulating agent ratio (S:EA) were selected as independent variables. Both designs were analyzed equally to evaluate differences between each carrying agent on polar compounds' encapsulation (process yield (Y%), encapsulation efficiency (EE%) and recovery of compounds (R%)) during the spray drying. The EE% and R% of each polar compound was monitored by High Performance Liquid Chromatography coupled to Time-of-Flight mass spectrometer by electrospray interface (HPLC-ESI-TOF-MS). The results showed that the use of IN as a carrier increased the powder recovered and the recovery of polar compounds after the spray dry process, whereas MD achieved a higher encapsulation efficiency.- Published
- 2020
- Full Text
- View/download PDF
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