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143 results on '"Liu Shangqin"'

1. Chemotherapy plus therapeutic plasmapheresis with 4% human albumin solution in multiple myeloma patients with acute kidney injury: a prospective, open-label, proof-of-concept study

Author

Wu, Tianzhi, primary, Liu, Dandan, additional, Liu, Shangqin, additional, Xiao, Hui, additional, Xiong, Bei, additional, Zhou, Yi, additional, Xiong, Yafen, additional, Cui, Qin, additional, Wu, Jiang, additional, Liu, Minghui, additional, Liu, Hongli, additional, Li, Yiming, additional, Wang, Meixin, additional, Bao, Xueqin, additional, Li, Ye, additional, and Zhou, Fuling, additional

Published
2024
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8. Infiltrating CD4 and CD8 lymphocytes in HPV infected uterine cervical milieu

Author

Maskey,Ninu, Thapa,Niresh, Maharjan,Muna, Shrestha,Girishma, Maharjan,Narayani, Cai,Hongbing, and Liu,Shangqin

Subjects
Cancer Management and Research, human papilloma virus, immunohistochemistry, CD8, cervical intraepithelial neoplasia, female genital diseases and pregnancy complications, cervical screening test, CD4, Original Research
Abstract

Ninu Maskey,1 Niresh Thapa,2,3 Muna Maharjan,4 Girishma Shrestha,5 Narayani Maharjan,6 Hongbing Cai,2 Shangqin Liu11Department of Hematology, Zhongnan Hospital of Wuhan University, Wuchang, Wuhan, Hubei 430071, People’s Republic of China; 2Department of Gynecological Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan 430071, People’s Republic of China; 3Karnali Academy of Health Sciences, Jumla, Nepal; 4Zhongnan Hospital of Wuhan University, Hope School of Nursing, Wuhan, Hubei, People’s Republic of China; 5Department of Pathology, Patan Hospital, Patan Academy of Health Sciences, Kathmandu, Nepal; 6Department of Clinical Laboratory Science, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of ChinaPurpose: Tumor infiltrating lymphocytes (TILs) have been extensively described in anti-tumor immunity, but their functional alterations in the immunoediting processes during neoplastic progression in the uterine cervix are still not clear. Our aim was to gain insight into cervical tissue T cell populations, determine if there are any differences in the localization and quantity distribution of T lymphocytes, and to evaluate their role in disease regression or progression in the cervical neoplastic milieu.Patients and methods: Serial section analysis of immunohistochemically stained CD4 and CD8 lymphocytes was performed on a total number of 72 samples, categorized into four cohorts: 23 HPV non-infected (HPV-) normal cervix, 20 HPV infected (HPV+) normal cervix, 17 HPV+ low grade cervical intraepithelial neoplasia (CIN), and 12 HPV+ high grade CIN.Results: Low infiltrating lymphocytes (ILs) in normal cervix and high ILs in CIN were observed, while the trend of ILs increased with increasing grade of CIN, which was statistically significant (P

Published
2019

10. PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

Author

Liu Shangqin, Wuhan Xiao, Hongzhu Yan, Ma Zi, Yuanpeng Sun, Zhe Wu, Wu Qian, Ning Zhifeng, Yanling Sun, Yusi Liu, Meichun Hu, and Liu Fuxing

Subjects
0301 basic medicine, Cancer Research, Cell, Biology, medicine.disease_cause, lcsh:RC254-282, 03 medical and health sciences, 0302 clinical medicine, Western blot, oncogene, medicine, PELP1, chlorpromazine, PI3K/AKT/mTOR pathway, Original Research, Oncogene, medicine.diagnostic_test, gastric cancer, therapeutic target, Cancer, master gene, Cell cycle, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, Oncology, Cell culture, 030220 oncology & carcinogenesis, Cancer research, c-Src-PI3K-ERK pathway, Carcinogenesis
Abstract

Background: Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1), a co-activator of estrogen receptors alpha, was confirmed to be directly associated with the oncogenic process of multiple cancers, especially hormone-dependent cancers. The purpose of our research was to explore the biological function, clinical significance, and therapeutic targeted value of PELP1 in gastric cancer (GC). Methods: The expression status of PELP1 in GC cell lines or tissues was analyzed through bioinformatics data mining. Thirty-six GC tissue chip was applied to demonstrate the results of bioinformatics data mining assayed by immunohistochemical method. The expression status of PELP1 in GC cell lines was also analyzed using western blot. Correlation analysis between PELP1 expression and clinicopathological parameter was performed. Kaplan-Meier survival analysis was applied to analyze the relationship between PELP1 expression and total survival time. Three pairs of siRNA were designed to silence the expression of PELP1 in GC. After PELP1 was silenced by siRNA or activated by saRNA, the growth, plate colony formation, migration and invasion ability of the GC cell or normal gastric epithelium cell line was tested in vitro. Cell cycle was tested by flow cytometry. Nude mice xenograft experiment was performed after PELP1 was silenced. The downstream molecular pathway regulated by PELP1 was explored. Molecular docking tool was applied to combine chlorpromazine with PELP1. The inhibitory effect of chlorpromazine in GC was assayed, then it was tested whether PELP1 was a therapeutic target of chlorpromazine in GC. Results: PELP1 expression was elevated in GC cell lines and clinical GC tissue samples. PELP1 silence by siRNA compromised the malignant traits of GC. PELP1 expression positively correlated with tumor invasion depth, lymph node metastasis, tissue grade, TNM stage, but had no correlation with patient age, sex, tumor size, and tumor numbers. Kaplan-Meier survival analysis revealed high PELP1 expression had a shorter survival period in GC patients after follow-up. Q-PCR and western blot revealed PELP1 suppression in GC decreased expression of the c-Src-PI3K-ERK pathway. It was also implied that chlorpromazine (CPZ) can inhibit the malignant traits of GC and downregulate the expression of PELP1. Conclusions: In a word, PELP1 is an oncogene in gastric cancer and c-Src-PI3K-ERK pathway activation may be responsible for its tumorigenesis, PELP1 may be a potential therapeutic target of chlorpromazine in GC.

Published
2020

21. Application of PAD class teaching mode in the teaching of Nursing Psychology.

Author

LI Li, LIU Chang, LIU Hang, ZHAO Qin, and LIU Shangqin

Abstract

Objective To explore the application effect of PAD class teaching mode in the teaching of Nursing Psychology. Methods TtTTwo classes of nursing undergraduates in academic year 2018 were divided into observation group (n =112) and control group (n = 107). The PAD CISSS teaching mode for the teaching of Nursing Psychology was adopted in the observation group, while the traditional teaching mode was adopted in the control group. The independent learning ability of the two groups was evaluated before and after the intervention, and the test scores of the two groups were compared at the end of the term. The students opinion on the PAD class teaching mode was investigated. Results After the intervention, the score of independent learning ability scale in the observation group (98.21 ±11.71) was higher than that of the control group (92.10 ± 13.77), with a significant difference (t = 2.382, P < 0. 05). The final score of the observation group (81.43 ±8.80) was higher than that of the control group (78.50 ±8.36), with a significant difference (5 = 2.527, D < 0.05). Students in the observation group held a positive attitude towards the PAD class teaching mode. Conclusion Compared with the traditional teaching mode, the PAD class teaching mode can optimize the classroom teaching effect and improve the independent learning ability and academic performance of students. [ABSTRACT FROM AUTHOR]

Published
2020
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22. A hospital based retrospective study of factors influencing therapeutic leukapheresis in patients presenting with hyperleukocytic leukaemia

Author

Jin, Yanxia, primary, Guo, Shishang, additional, Cui, Qin, additional, Chen, Sichao, additional, Liu, Xiaoping, additional, Wei, Yongchang, additional, Pan, Yunbao, additional, Tang, Liang, additional, Huang, Tingting, additional, Shen, Hui, additional, Xu, Guanghui, additional, Zuo, Xuelan, additional, Liu, Shangqin, additional, Xiao, Hui, additional, Chen, Fei, additional, Gong, Fayun, additional, and Zhou, Fuling, additional

Published
2018
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27. An integrated technique for identification of differential genes expressed in patients with cancer

Author

Lei Xiaomci, Li Shouxin, and Liu Shangqin

Subjects
Cloning, education.field_of_study, Differential display, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Population, Biomedical Engineering, DNA, Biology, Biochemistry, Molecular biology, Biomaterials, Gene Expression Regulation, Neoplastic, Cell culture, Suppression subtractive hybridization, Complementary DNA, Cell Line, Tumor, Gene expression, Genetics, Humans, Leukocyte Common Antigens, education, Multiple Myeloma, Gene, Earth-Surface Processes
Abstract

To develop a method for identification of differential gene expression between different cell population, several conveninent techniques of molecular biology, including subtractive hybridization, suppression PCR, T/A cloning and sequencing, were used to identify genes expressed differentially in CD45− and CD45− cells isolated from U266 cell line of multiple myeloma. Our results showed that the levels of abundant genes genes scale down 20 times through subtractive hybridization. Plasmid DNA from CD45 cell clones was hybridized with forward or backward cDNA probes synthesized from CD45 and CD45 cells, respectively. A few of differentially expressed genes reconfirmed by RT-PCR were identified from 500 expressed clones of CD45′ cells. It is concluded that a strategy for gene expression identification developed from conventional molecular biological methods can be used in different laboratories.

Published
2005

29. Autophagy Pathway Is Required for IL-6 Induced Neuroendocrine Differentiation and Chemoresistance of Prostate Cancer LNCaP Cells

Author

Chang, Pei-Ching, primary, Wang, Tao-Yeuan, additional, Chang, Yi-Ting, additional, Chu, Cheng-Ying, additional, Lee, Chin-Ling, additional, Hsu, Hung-Wei, additional, Zhou, Tyng-An, additional, Wu, Zhaoju, additional, Kim, Randie H., additional, Desai, Sonal J., additional, Liu, Shangqin, additional, and Kung, Hsing-Jien, additional

Published
2014
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38. Dehydroepiandrosterone Can Inhibit the Proliferation of Myeloma Cells and the Interleukin-6 Production of Bone Marrow Mononuclear Cells from Patients with Myeloma

Author

Liu, Shangqin, primary, Ishikawa, Hideaki, additional, Li, Fu-Jun, additional, Ma, Zi, additional, Otsuyama, Ken-ichiro, additional, Asaoku, Hideki, additional, Abroun, Saeid, additional, Zheng, Xu, additional, Tsuyama, Naohiro, additional, Obata, Masanori, additional, and Kawano, Michio M., additional

Published
2005
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46. Baicalein, a component of Scutellariaradix from Huang-Lian-Jie-Du-Tang (HLJDT), leads to suppression of proliferation and induction of apoptosis in human myeloma cells

Author

Ma, Zi, Otsuyama, Ken-ichiro, Liu, Shangqin, Abroun, Saeid, Ishikawa, Hideaki, Tsuyama, Naohiro, Obata, Masanori, Li, Fu-Jun, Zheng, Xu, Maki, Yasuko, Miyamoto, Koji, and Kawano, Michio M.

Abstract

In the search for a more effective adjuvant therapy to treat multiple myeloma (MM), we investigated the effects of the traditional Chinese herbal medicines Huang-Lian-Jie-Du-Tang (HLJDT), Gui-Zhi-Fu-Ling-Wan (GZFLW), and Huang-Lian-Tang (HLT) on the proliferation and apoptosis of myeloma cells. HLJDT inhibited the proliferation of myeloma cell lines and the survival of primary myeloma cells, especially MPC-1-immature myeloma cells, and induced apoptosis in myeloma cell lines via a mitochondria-mediated pathway by reducing mitochondrial membrane potential and activating caspase-9 and caspase-3. Further experiments confirmed that Scutellariaradix was responsible for the suppressive effect of HLJDT on myeloma cell proliferation, and the baicalein in Scutellariaradix showed strong growth inhibition and induction of apoptosis in comparison with baicalin or wogonin. Baicalein as well as baicalin suppressed the survival in vitro of MPC-1-immature myeloma cells rather than MPC-1+myeloma cells from myeloma patients. Baicalein inhibited the phosphorylation of IkB-α, which was followed by decreased expression of the IL-6and XIAPgenes and activation of caspase-9 and caspase-3. Therefore, HLJDT and Scutellariaradix have an antiproliferative effect on myeloma cells, especially MPC-1-immature myeloma cells, and baicalein may be responsible for the suppressive effect of Scutellariaradix by blocking IkB-α degradation. (Blood. 2005;105:3312-3318)

Published
2005
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50. An integrated technique for identification of differential genes expressed in patients with cancer.

Author

Li S, Liu S, and Lei X

Subjects
Cell Line, Tumor, DNA genetics, Gene Expression Profiling, Humans, Multiple Myeloma genetics, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Regulation, Neoplastic, Leukocyte Common Antigens genetics, Multiple Myeloma pathology
Abstract

To develop a method for identification of differential gene expression between different cell populations, several convenient techniques of molecular biology, including subtractive hybridization, suppression PCR, T/A cloning and sequencing, were used to identify genes expressed differentially in CD45+ and CD45- cells isolated from U266 cell line of multiple myeloma. Our results showed that the levels of abundant genes scale down 20 times through subtractive hybridization. Plasmid DNA from CD45- cell clones was hybridized with forward or backward cDNA probes synthesized from CD45- and CD45- cells, respectively. A few of differentially expressed genes reconfirmed by RT-PCR were identified from 500 expressed clones of CD45+ cells. It is concluded that a strategy for gene expression identification developed from conventional molecular biological methods can be used in different laboratories.

Published
2004
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