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Summary: Hyperfunction of the dopamine system has been implicated in manic episodes in bipolar disorders. How dopaminergic neuronal function is regulated in the pathogenesis of mania remains unclear. Histaminergic neurons project dense efferents into the midbrain dopaminergic nuclei. Here, we present mice lacking dopaminergic histamine H2 receptor (H2R) in the ventral tegmental area (VTA) that exhibit a behavioral phenotype mirroring some of the symptoms of mania, including increased locomotor activity and reduced anxiety- and depression-like behavior. These behavioral deficits can be reversed by the mood stabilizers lithium and valproate. H2R deletion in dopaminergic neurons significantly enhances neuronal activity, concurrent with a decrease in the γ-aminobutyric acid (GABA) type A receptor (GABAAR) membrane presence and inhibitory transmission. Conversely, either overexpression of H2R in VTA dopaminergic neurons or treatment of H2R agonist amthamine within the VTA counteracts amphetamine-induced hyperactivity. Together, our results demonstrate the engagement of H2R in reducing VTA dopaminergic activity, shedding light on the role of H2R as a potential target for mania therapy.

This study extensively investigates 4,557 articles pertaining to immigrants’ cross-cultural adaptation (ICCA) published between 1963 and 2022 in Web of Science (WoS)-core collection database by presenting a state-of-the-art bibliometric analysis and visualizing knowledge maps with a set of analytical tools including VOSviewer, CiteSpace, and Bibliometrix. The collected sample covers 9,791 authors, 1,473 sources, 81 countries and regions, and 2,682 institutions. The major aim of the study is to explore the research status and progress on a global scale in terms of different parameters in a quantitative and visual manner and discern the overall development in research hotspots. The research findings reveal that publications on ICCA have registered marked increases over time and that the degree of research varies remarkably in countries/territories, authors, institutions, and sources. A host of scholars are predominantly focused on “acculturation,”“integration,” and “attitude” in addition to “immigrant” themselves, whereas some started to take up research on emerging topics like “social network,”“national identity,”“Syrian refugee,”“resilience,”“social integration,”“work,” and “employment.” Future research directions on ICCA research have been justified accordingly. To the best of our knowledge, our study is the first review of its kind in the field and offers multiple influential research avenues, which may provide unique and essential perspectives and ideas for scholars to carry out relevant research down the road. It is hoped that this study is expected to stir up more research interest in ICCA research.

Abstract Background A growing body of literature demonstrates that social media usage has witnessed a rapid increase in higher education and is almost ubiquitous among young people. The underlying mechanisms as to how social media usage by university students affects their well-being are unclear. Moreover, current research has produced conflicting evidence concerning the potential effects of social media on individuals' overall well-being with some reporting negative outcomes while others revealing beneficial results. Methods To address the research gap, the present research made an attempt to investigate the crucial role of social media in affecting students’ psychological (PWB) and subjective well-being (SWB) by testing the mediating role of self-esteem and online social support and the moderation effect of cyberbullying. The data in the study were obtained from a sample of 1,004 college students (483 females and 521 males, M age = 23.78, SD = 4.06) enrolled at 135 Chinese universities. AMOS 26.0 and SPSS 26.0 as well as the Process macro were utilized for analyzing data and testing the moderated mediation model. Results Findings revealed that social media usage by university students was positively associated with their PWB and SWB through self-esteem and online social support, and cyberbullying played a moderating role in the first phase of the mediation process such that the indirect associations were weak with cyberbullying reaching high levels. Conclusion These findings highlight the importance of discerning the mechanisms moderating the mediated paths linking social media usage by young adults to their PWB and SWB. The results also underline the importance of implementing measures and interventions to alleviate the detrimental impacts of cyberbullying on young adults’ PWB and SWB.

As the volume of research on intercultural adaptation of international students (IAIS) increases significantly over the past decades, a systematic review of the diverse literature on a global scale and its development process becomes essential. This study conducted a thorough bibliometric analysis for the knowledge domain of IAIS research using relevant articles ( N = 1,126) from 1990 to 2022 in Thompson Reuter’s Web of Science (WoS) database-Core Collection. This work aims to analyze the advances and trends on the subject using a set of bibliometric analytical tools, including Bibliometrix, VOSviewer, and BibExcel. The research findings demonstrate that the number of published articles projects an ostensible upward trend in terms of temporal distribution, especially since 2007. The USA occupies a leading position in terms of the largest publication output and a greater influence therewith. The most productive journal, author(s), and institution(s) are International Journal of Intercultural Relations , Brown L and Wang KT, and the University of Hong Kong and the University of Queensland, respectively. Collaboration in this field has become mainstream but still needs to be enhanced among researchers and at national and institutional levels. The frequency and co-occurrence analysis of keywords reveals that the main keywords for discussion include international students, adjustment, acculturation, adaptation, acculturative stress, identity, social support, stress, experiences, and depression, while cultural intelligence and intercultural sensitivity, culture shock and intercultural challenges, and integration and migration serve as the emerging themes. The study would aid academic researchers and policymakers in understanding the intricacies of IAIS and identifying areas that require further investigation.

Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%.

Grass carp hemorrhagic disease caused by grass carp reovirus (GCRV) results in significant economic losses to the global grass carp aquaculture industry. Oral vaccination is an ideal choice for disease precaution in aquaculture. However, oral vaccine can be degraded in the gut. Therefore, the selection of loading materials is essential. In this study, the S6 and S7 fragments (encoding the outer capsid protein VP4 and fibronectin VP56 of GCRV) and grass carp interferons (IFNs), including IFN1, IFN3, and IFNγ2 were used to create DNA vaccines and adjuvants based on pcDNA3.1, respectively. The oral DNA vaccine was encapsulated in poly(lactic-co-glycolic acid) (PLGA) and polyvinyl alcohol (PVA) with IFNs. The PLGA-PVA (PP) nano-microspheres were prepared by double emulsion-solvent evaporation technique. Using transmission electron microscopy and dynamic light scattering assays, it was determined the vaccines had a spherical structure with uniform particle size (643.5 ​± ​35.3 ​nm). The nano-microspheres possessed excellent encapsulation efficiency (81.6 ​± ​2.6%) and loading rate (0.54 ​± ​0.02%), and simultaneously exhibited negligible hemolytic activity and cell toxicity. The protection rate and tissue viral loads post-GCRV challenge in grass carp were assessed. The oral PP nano-microsphere with pVP4 ​+ ​pIFN1 (PP41) vaccine increased protection rate by 44% compared with the control group and was correlated with relatively low viral loads in the spleen, head kidney, and hindgut. Further, three crucial serum biochemical indexes, total superoxide dismutase (TSOD), complement C3 (C3), and lysozyme (LZM), were also dramatically increased. Furthermore, mRNA expressions of representative immune-related genes (IgM, IFN1, IFNγ2, MHC-Ⅰ, and CD8α) in the head kidney and spleen were significantly enhanced. In addition, mRNA expression of IgT was significantly boosted in the hindgut. The results indicate that DNA vaccine capsulated with PP is effective against GCRV infection. The present study provides insights into a prospective strategy for oral vaccine development in aquaculture.

International students studying and living in a foreign context often complain about difficulties establishing friendships with host nationals. This study investigates host country nationals’ (HCNs) willingness to develop intercultural friendships with international students who are sojourning in China by exploring the effects of face-to-face and online intergroup contact, HCNs’ attitudes, intercultural communication competence (ICC), and perceived intergroup threats. Survey data from 469 HCNs indicate that (a) face-to-face and online contact are indirectly and positively related to their willingness to form intercultural friendships, (b) face-to-face contact can moderate the relationships of online contact with HCNs’ intergroup attitudes and perceived intergroup threats, and (c) both ICC and intergroup attitudes can positively predict friendship formation whereas perceived intergroup threats act as a negative predictor. The implications of our findings for future research and practice are presented.