Caenibaculum baiyandianus gen. nov., sp. nov., a New Bacterium Isolated from Sewage Sediment of Baiyangdian

An aerobic, gram-negative-staining, motile and rod-shaped bacterium, designated strain 3-BNT, was isolated from sewage sediment collected from Baiyangdian, located in Xiongan New Area of China. This heterotrophic bacterium grows on glucose, yeast extract and a limited range of defined organic acids including lactic and propionic acids. The major isoprenoid quinone is ubiquinone Q-8. Major polar lipids are phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidyl glycerol (DPG) and three unidentified glycolipids. The major fatty acids are C16:0 (30.9%), C18:1ω7c (18.3%), C17:0 cyclo (16.1%) and C14:0 (7.9%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 3-BNT belongs to the family Burkholderiaceae, and its closest phylogenetic neighbors are members of the genera Burkholderia and Caballeronia. However, strain 3-BNT did not make a coherent clade with members of the currently recognized genera. Based on phylogenetic and chemotaxonomic characteristics of this bacterium, a novel genus and species, Caenibaculum baiyandianus gen. nov., sp. nov., is proposed. The type strain is 3-BNT (=ACCC 60082T=KCTC 62431T).