1. Novel PCR Assay for Determining the Genetic Sex of Mice
- Author
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Dagmar Wilhelm, Lindsey McFarlane, Vy Truong, and James S. Palmer
- Subjects
Male ,Genetics ,Sex Determination Analysis ,Embryology ,Sexual differentiation ,Base Sequence ,Endocrinology, Diabetes and Metabolism ,Molecular Sequence Data ,Nuclear Proteins ,Mice, Inbred Strains ,Sexing ,Sex reversal ,Amplicon ,Biology ,Y chromosome ,Polymerase Chain Reaction ,Molecular biology ,Mice ,Animals ,Female ,Primer (molecular biology) ,Pseudogenes ,X chromosome ,Developmental Biology - Abstract
A number of studies require the determination of the genetic sex of mouse embryos before sexual differentiation and/or of mutant mice that display partial or complete sex reversal. The majority of current methods for sexing by PCR involve multiplexing of 2 primer pairs. We have developed a novel sexing PCR using a single primer pair that amplifies fragments from the X and the Y chromosome with a clear size difference between the respective amplicons. This assay provides a rapid and reliable method to identify the genetic sex of mice across different mouse strains.
- Published
- 2013