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Novel PCR Assay for Determining the Genetic Sex of Mice

Authors :
Dagmar Wilhelm
Lindsey McFarlane
Vy Truong
James S. Palmer
Source :
Sexual Development. 7:207-211
Publication Year :
2013
Publisher :
S. Karger AG, 2013.

Abstract

A number of studies require the determination of the genetic sex of mouse embryos before sexual differentiation and/or of mutant mice that display partial or complete sex reversal. The majority of current methods for sexing by PCR involve multiplexing of 2 primer pairs. We have developed a novel sexing PCR using a single primer pair that amplifies fragments from the X and the Y chromosome with a clear size difference between the respective amplicons. This assay provides a rapid and reliable method to identify the genetic sex of mice across different mouse strains.

Details

ISSN :
16615433 and 16615425
Volume :
7
Database :
OpenAIRE
Journal :
Sexual Development
Accession number :
edsair.doi.dedup.....0c29bc52a1138b0f89fe1be52240e224