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Orchestrated action of peptidoglycan (PG) synthetases and hydrolases is vital for bacterial growth and viability. Although the function of several PG synthetases and hydrolases is well understood, the function, regulation, and mechanism of action of PG hydrolases characterised as lysostaphin-like endopeptidases have remained elusive. Many of these M23 family members can hydrolyse glycyl-glycine peptide bonds and show lytic activity against Staphylococcus aureus whose PG contains a pentaglycine bridge, but their exact substrate specificity and hydrolysed bonds are still vaguely determined. In this work, we have employed NMR spectroscopy to study both the substrate specificity and the bond cleavage of the bactericide lysostaphin and the S. aureus PG hydrolase LytM. Yet, we provide substrate-level evidence for the functional role of these enzymes. Indeed, our results show that the substrate specificities of these structurally highly homologous enzymes are similar, but unlike observed earlier both LytM and lysostaphin prefer the D-Ala-Gly cross-linked part of mature peptidoglycan. However, we show that while lysostaphin is genuinely a glycyl-glycine hydrolase, LytM can also act as a D-alanyl-glycine endopeptidase.

This research aims to consider pointed to decide the steps in creating Lectora Inspire learning media and its possibility for arithmetic learning of divisions for third-grade basic school understudies. The investigation was investigation and improvement. The advancement forms concerned the improvement show proposed by Thiagarajan's 4D. The 4D development model included the stages of (1) Define, (2) Design, (3) Development, and (4) Dissemination. The information collection rebellious utilized were master appraisal sheets, instructor evaluation sheets, and understudy appraisal sheets. The result about appeared that Lectora inspire media gotten a rate of 77.3% within the "great" category by media specialists, 88% within the "outstanding" category by material specialists, and 86.6% within the "extraordinary" category by learning specialists. The normal rate from specialists was 83.96%. The result of the teacher's appraisal gotten a rate of 96% within the "exceptional" category. The result of the restricted trial evaluation gotten a rate of 100% within the "exceptional" category. This information demonstrated that the Lectora inspire media for arithmetic learning of divisions for third-grade understudies of the rudimentary school had a fabulous and doable quality. These data indicated that the Lectora Inspire media for mathematics learning of fractions for third-grade students of elementary school had an excellent quality and was feasible.

Abstract Background Falls are common in the elderly and can lead to adverse consequences, like injuries, hospitalization, disability even mortality. Successful ageing emerged in sight to assess physical, psychological and social status of older adults. This study is conducted to explore the association between them in a large Indian community-dwelling population. Methods Data were based on the wave 1 survey of the Longitudinal Ageing Study in India (LASI). People aged 60 and above with complete information were included. The elderly met five standards including absence of chronic diseases, freedom from disability, high cognitive ability, free from depressive symptoms and active social engagement, were classified into successful agers. The assessment of falls, fall-related injuries and multiple falls depended on interview. Multivariate logistic regression was conducted to find the associations between falls, fall-injury, multiple falls and successful ageing after adjusting both socio-demographic and biological covariates. The log-likelihood ratio test was calculated interactions in subgroups. Results 31,345 participants in LASI were finally included in our study. Of them, 20.25% reported fall, and 25% were classified into successful agers. After full adjustment, successful ageing was negatively associated with falls (OR 0.70; 95%CI 0.65–0.76) and multiple falls (OR 0.70; 95%CI 0.63–0.78). And the association did not show the significance in older adults with fall-related injuries (OR 0.86; 95%CI 0.72–1.04). Conclusions Successful ageing was negatively associated with falls and multiple falls, but not fall-related injuries in older people in India. Future studies are demanded to explore the causal relationship and to reveal the underlying mechanism.

Abstract Congenital diaphragmatic hernia (CDH) is a birth defect characterized by incomplete closure of the diaphragm, herniation of abdominal organs into the chest, and compression of the lungs and the heart. Besides complications related to pulmonary hypoplasia, 1 in 4 survivors develop neurodevelopmental impairment, whose etiology remains unclear. Using a fetal rat model of CDH, we demonstrated that the compression exerted by herniated organs on the mediastinal structures results in decreased brain perfusion on ultrafast ultrasound, cerebral hypoxia with compensatory angiogenesis, mature neuron and oligodendrocyte loss, and activated microglia. In CDH fetuses, apoptosis was prominent in the subventricular and subgranular zones, areas that are key for neurogenesis. We validated these findings in the autopsy samples of four human fetuses with CDH compared to age- and sex-matched controls. This study reveals the molecular mechanisms and cellular changes that occur in the brain of fetuses with CDH and creates opportunities for therapeutic targets.

Multiple sclerosis (MS) is a devastating immune-mediated disorder of the central nervous system resulting in progressive disability accumulation. As there is no cure available yet for MS, the primary therapeutic objective is to reduce relapses and to slow down disability progression as early as possible during the disease to maintain and/or improve health-related quality of life. However, optimizing treatment for people with MS (pwMS) is complex and challenging due to the many factors involved and in particular, the high degree of clinical and sub-clinical heterogeneity in disease progression among pwMS. In this paper, we discuss these many different challenges complicating treatment optimization for pwMS as well as how a shift towards a more pro-active, data-driven and personalized medicine approach could potentially improve patient outcomes for pwMS. We describe how the ‘Clinical Impact through AI-assisted MS Care’ (CLAIMS) project serves as a recent example of how to realize such a shift towards personalized treatment optimization for pwMS through the development of a platform that offers a holistic view of all relevant patient data and biomarkers, and then using this data to enable AI-supported prognostic modelling.

Background: Men’s access to sexual health care is influenced by the nurses’ symbolic universes, translated into maps of signification and normative idealization that structure their practices. Aim: The aim of the study was to analyze nurses’ social representations of men’s sexual health, considering the barriers and possibilities in accessing health care. Methods: Descriptive study with qualitative approach. Data were collected through an online focus group with 9 Portuguese nurses. Data were subjected to lexicographic analysis with the support of the interface IRaMuTeQ-R. Results: 269 text segments were analyzed, retaining 84.86% of the total in 4 classes: interactions between professionals and men, therapeutic itineraries, “talking” about sexual health, and men’s access to sexual health care. Similarity analysis led to 3 central cores: problem, saying, and patient. Conclusions: The training of professionals in men’s sexual health to demystify stereotypes about masculinity is fundamental. At the organizational level, it is necessary to analyze the possibilities of access and its visibility in electronic records and rethink the planning and provision of primary and differentiated care.

Abstract Background Previous studies have reported that inflammatory responses can promote the onset of cardiovascular diseases; however, its association with cardiac conduction disorders remains unclear. The present community-based cohort study aimed to elucidate the effects of inflammatory responses on the risk of developing cardiac conduction disorders. Methods After the exclusion of participants failing to meet the inclusion criteria, 86,234 eligible participants (mean age: 50.57 ± 11.88 years) were included. The participants were divided into high-sensitivity C-reactive protein (hsCRP) ≤ 3 mg/L, and hsCRP > 3 mg/L groups based on hsCRP values. Multivariate Cox proportional hazard model was used to analyze the relationship between inflammatory responses and various cardiac conduction disorders. Results After adjusting for confounding factors, we observed that compared with the hsCRP ≤ 3 mg/L group, the hsCRP > 3 mg/L group exhibited increased risks of atrioventricular block (hazard ratio [HR]:1.64, 95%confidence interval [CI] 1.44–1.87) and left (HR:1.25, 95% CI 1.07–1.45) and right bundle branch block (HR:1.31, 95% CI 1.17–1.47). Moreover, the risk of various cardiac conduction disorders increased for every 1 standard deviation increase in log (hsCRP). The restricted cubic spline function confirmed a linear relationship between log (hsCRP) and the risk of developing cardiac conduction disorders (All nonlinearity P > 0.05). Conclusions High hsCRP levels are an independent risk factor for cardiac conduction disorders, and hsCRP levels are dose-dependently associated with the risk of conduction disorders. Our study results may provide new strategies for preventing cardiac conduction disorders.

Summary: Background: The Paediatric Difficult Intubation Collaborative identified multiple attempts and persistence with direct laryngoscopy as risk factors for complications in children with difficult tracheal intubations and subsequently engaged in initiatives to reduce repeated attempts and persistence with direct laryngoscopy in children. We hypothesised these efforts would lead to fewer attempts, fewer direct laryngoscopy attempts and decrease complications. Methods: Paediatric patients less than 18 years of age with difficult direct laryngoscopy were enrolled in the Paediatric Difficult Intubation Registry. We define patients with difficult direct laryngoscopy as those in whom (1) an attending or consultant obtained a Cormack Lehane Grade 3 or 4 view on direct laryngoscopy, (2) limited mouth opening makes direct laryngoscopy impossible, (3) direct laryngoscopy failed in the preceding 6 months, and (4) direct laryngoscopy was deferred due to perceived risk of harm or poor chance of success. We used a 5:1 propensity score match to compare an early cohort from the initial Paediatric Difficult Intubation Registry analysis (August 6, 2012–January 31, 2015, 785 patients, 13 centres) and a current cohort from the Registry (March 4, 2017–March 31, 2023, 3925 patients, 43 centres). The primary outcome was first attempt success rate between cohorts. Success was defined as confirmed endotracheal intubation and assessed by the treating clinician. Secondary outcomes were eventual success rate, number of attempts at intubation, number of attempts with direct laryngoscopy, the incidence of persistence with direct laryngoscopy, use of supplemental oxygen, all complications, and severe complications. Findings: First-attempt success rate was higher in the current cohort (42% vs 32%, OR 1.5 95% CI 1.3–1.8, p

Introdução: As representações sociais sobre o que é saúde sexual são produzidas, transformadas e reconfiguradas quotidianamente pela ação dos indivíduos, pelas suas experiências nas relações com os outros, e pelas interações sociais em diversos contextos mediados por normas e papéis sociais (Moscovici, 1984; Herzlich, 1992; Jodelet, 1989). Os universos simbólicos dos enfermeiros traduzem-se em mapas de significação e idealização normativa, que estruturam as suas práticas e influenciam o acesso dos homens aos cuidados de saúde sexual. Objetivo: Analisar as representações sociais dos enfermeiros sobre saúde sexual dos homens considerando as possibilidades e os constrangimentos destes no acesso aos cuidados de saúde através da utilização do IRaMuTeQ. Material e Métodos: Estudo qualitativo e descritivo com a realização de um Focus Group online (Morgan, 2019). Na procura de heterogeneidade no grupo de participantes, foram convidados 5 enfermeiras e 4 enfermeiros portugueses com diferentes tempos de exercício profissional nos cuidados de saúde primários e diferentes tipos de serviços dos cuidados de saúde diferenciados. A entrevista de grupo foi transcrita e preparada em corpus textual para a análise lexicográfica, com recurso ao IRaMuTeQ - Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires, um software gratuito, com fonte aberta para análise de dados textuais (Camargo & Justo, 2013; 2018). Foi solicitado parecer ao Conselho de Ética da ESEL e foi obtido o consentimento informado de todos os participantes. Resultados: Análise de 269 segmentos de texto, retendo 84,86% do total para 4 classes: interações entre os profissionais e os homens, itinerários terapêuticos, “falar” sobre saúde sexual e acesso dos homens aos cuidados de saúde sexual. A análise de similitude originou 3 núcleos centrais: problema, dizer e doente. Conclusões: Possibilitar o acesso dos homens aos cuidados de saúde sexual, requer o planeamento e a realização de formação dos profissionais em saúde sexual em geral e sobre os homens em particular. Requer, ainda, uma abordagem positiva da saúde sexual e uma convergência de esforços das políticas de saúde, das instituições formativas, dos investigadores, dos profissionais de saúde e das comunidades em geral para desmistificar crenças e estereótipos sobre a sexualidade, a masculinidade e clarificar a inclusão dos homens como sujeitos de cuidados.

This research aimed to investigate the translation strategies employed by 5th semester students of the English Language Study Program at Sembilanbelas November Kolaka University when translating narrative texts. The authors sought to understand the students' approaches to translating these texts, as well as identify the errors they made throughout the process. By doing so, the authors hoped to provide valuable insights to students regarding the most effective translation strategies and assist them in recognizing and correcting their translation errors. A qualitative research design was utilized to examine the translation strategies of narrative texts. Data collection involved a translation test taken by 30 students, which served as the foundation for the authors' analysis. Based on the findings, the authors concluded that the students mainly implemented three translation strategies to translate narrative texts. The first strategy was literal translation which was used 84% of the time. Literal translation strategy means that the translators translate texts as is. The second strategy that was linguistic amplification. It was used 2.41% of the time. Linguistic amplification is a translation strategy where translators add more details into the TL. The third strategy that was used was generalization at 2.29%. Using this strategy means that the translators decide to use the general term of a word instead of translating it specifically based on the SL. The authors also found out that the most common errors that the students made while translating the texts they were given was incorrect word errors. In other words, the students incorrectly translated the words from the SL to the TL which may cause confusion for the readers. By doing this research it is expected become an insight about language teaching especially on the translation related to translation strategi that used to translate. The appropriate implementation of translation method in translating process will produce accurate translation. The findings of this study underscore the importance of providing targeted instruction on translation strategies for EFL students. By doing so, educators can help students develop the skills and knowledge necessary to produce high-quality translations that effectively convey the meaning and nuances of the source text.

Wooden breast (WB) myopathy is a pathology of the pectoralis major muscle. Wooden breast is caused by multiple factors. The exact etiopathogenesis of this myodegenerative pathology is still unclear. Fast-growing commercial lines of broilers that are selected for high breast muscle yields are more susceptible to this myopathy. The biochemical analysis of blood is used to diagnose pathologies and understand disease processes. Therefore, the objective of this research was to determine and compare the changes in the blood serum biochemical parameters of Ross 308 chicken broilers without myopathy and those affected by WB myopathy. Blood samples were collected from male and female Ross 308 broilers that were 43 days old, with an average live weight of 2.98–3.09 kg. Representative blood samples were selected from broilers with WB (n = 33) and without WB (n = 33). In the laboratory, the blood was centrifugated, and biochemical tests were performed with an automated computerized biochemistry analyzer. The research results showed that broilers with WB had elevated blood serum levels of creatine kinase (CK) (p = 0.018), potassium (p = 0.010), and alanine aminotransferase (ALT) (p = 0.012). In conclusion, elevated serum levels of CK and potassium indicated that skeletal muscle cells were damaged. Moreover, increased ALT levels suggested a possible association between WB myopathy and liver damage. Additionally, these research findings underscore the diagnostic significance of CK and hint at its potential as a WB biomarker.

This study hypothesizes that heat stress adversely affects dairy cows, resulting in reduced rumination, altering eating and drinking behaviors, changes in their locomotory patterns, and significant variations in their acid–base balance. The aim of this study was to investigate the impacts of heat stress on rumination, drinking, and locomotory behavior, as registered by innovative technologies, and acid–base balance in fresh multiparous dairy cows. This study was conducted during the summer, from 15 June to 8 July 2023, on a Lithuanian commercial dairy farm. We assessed 350 German Holstein cows that produced an average of 11,400 kg of milk annually throughout their second and subsequent lactation periods. We used the temperature–humidity index (THI) to divide the cows under investigation into three periods: I. high HS—THI >78 (period: 15–23 June 2023); II. medium HS—THI 72–78 (period: 24–30 June 2023); and III. low HS—THI p < 0.01); high mean differences were detected for rumination, which was 17.67% higher in the high HS period and 13.80% higher in the medium HS period compared to the low HS period (p < 0.01); and the change in activity was 12.82% higher in the low HS compared to the medium HS period (p < 0.01). Cows under high HS had higher blood urea nitrogen (BUN) levels compared with cows under medium HS (p < 0.01). The observed alterations in the rumination, drinking, and locomotory behaviors, in addition to the acid–base balance, highlight the multifaceted impacts of varying heat stress on the physiological and behavioral responses of dairy cows. This suggests that the utilization of advanced technologies may assist dairy farmers in effectively monitoring and controlling heat stress in cows. Additionally, regularly assessing blood urea nitrogen levels can enable farmers to modify their feeding practices, thus promoting optimal cow well-being and productivity.

Benign prostatic hyperplasia (BPH) is an androgen-related non-neoplastic enlargement of the prostate gland that commonly affects both reproductive capabilities and the general health of intact dogs. The subclinical form of BPH can be challenging to diagnose due to a lack of clinical signs, even if rectal palpation is performed. Left untreated, this condition poses risks to the dogs’ health and breeding status. This study, involving 65 male dogs, aimed to investigate subclinical BPH through rectal palpation, ultrasonography, and analysis of canine prostatic-specific esterase (CPSE). Of the participants, 35 had subclinical BPH, and 30 served as a healthy control group. Dogs suspected of subclinical BPH, as determined by examination results from ultrasonography and CPSE analysis, underwent fine needle aspiration (FNA) guided by ultrasound to enhance diagnostic precision. Findings revealed distinct differences in rectal palpation and ultrasonography between subclinical BPH and healthy dogs. This study established diagnostic thresholds based on prostatic volume and CPSE values and proposed new thresholds for subclinical BPH. Additionally, results showed that prostate gland volume depended on the weight and the age of the dog. In conclusion, early detection of this condition is possible through various examinations, such as changes in ultrasound features, CPSE levels, and rectal palpation. All together, these methods can aid practitioners in early detection of BPH and assist with scheduling screening programs for dogs, ultimately promoting their overall health and reproductive well-being. In conclusion, we advocate for routine, non-invasive prostate screenings in breeding males, underlining the effectiveness of a combination of various multiple techniques for early subclinical BPH detection.

This systematic review aims to assess the effectiveness of virtual reality (VR) and gamification interventions in addressing anxiety and depression. The review also seeks to identify gaps in the current VR treatment landscape and provide guidelines for future research and development. A systematic literature search was conducted using Scopus, Web of Science, and PubMed databases, focusing on studies that utilized VR and gamification technology to address anxiety and depression disorders. A total of 2,664 studies were initially identified, 15 of those studies fulfilled the inclusion criteria for this systematic review. The efficacy of VR in addressing anxiety and depression was evident across all included studies. However, the diversity among VR interventions highlights the need for further investigation. It is advised to incorporate more diverse participant samples and larger cohorts and explore a broader spectrum of therapeutic approaches within VR interventions for addressing anxiety and depression to enhance the credibility of future research. Additionally, conducting studies in varying socioeconomic contexts would contribute to a more comprehensive understanding of their real-world applicability.

Purpose The aim of this study was to conduct a scoping review of knowledge and information delivery modes related to preconception care (PCC) among adolescent girls and women. Methods A scoping review was performed on studies selected from five electronic databases (Cochrane Library, PubMed, Science Direct, CINAHL/EBSCO, and ProQuest), published between 2012 and 2022, with predetermined keywords and criteria. We included English-language research articles available in full text and excluded irrelevant articles. Results This study included eight articles, comprising seven quantitative studies and one qualitative study conducted among adolescent girls and women. Five were from low- and middle-income countries and three were from high-income countries. The synthesized themes generated from the data were PCC knowledge and PCC information delivery modes and effectiveness. In general, adolescent girls and women were found to have basic PCC knowledge, including risk prevention and management and a healthy lifestyle, although more extensive knowledge was found in higher-income countries than in lower-income countries. The delivery modes of PCC information have grown from individual face-to-face conventional methods, which are used predominantly in lower-income countries, to more effective digital mass media. Conclusion Globally, many women still have insufficient knowledge regarding PCC, as not all of them receive access to PCC information and support. PCC promotion efforts should be initiated earlier by involving a wider group of reproductive-age women and combining individual, in-group, face-to-face, and electronic delivery modes.

Introduction There is uncertainty about the advantages and disadvantages of laparoscopic hysterectomy compared with abdominal hysterectomy, particularly the relative rate of complications of the two procedures. While uptake of laparoscopic hysterectomy has been slow, the situation is changing with greater familiarity, better training, better equipment and increased proficiency in the technique. Thus, a large, robust, multicentre randomised controlled trial (RCT) is needed to compare contemporary laparoscopic hysterectomy with abdominal hysterectomy to determine the safest and most cost-effective technique.Methods and analysis A parallel, open, non-inferiority, multicentre, randomised controlled, expertise-based surgery trial with integrated health economic evaluation and an internal pilot with an embedded qualitative process evaluation. A within trial-based economic evaluation will explore the cost-effectiveness of laparoscopic hysterectomy compared with open abdominal hysterectomy. We will aim to recruit 3250 women requiring a hysterectomy for a benign gynaecological condition and who were suitable for either laparoscopic or open techniques. The primary outcome is major complications up to six completed weeks postsurgery and the key secondary outcome is time from surgery to resumption of usual activities using the personalised Patient-Reported Outcomes Measurement Information System Physical Function questionnaire. The principal outcome for the economic evaluation is to be cost per QALY at 12 months’ postsurgery. A secondary analysis is to be undertaken to generate costs per major surgical complication avoided and costs per return to normal activities.Ethics and dissemination The study was approved by the West Midlands-Edgbaston Research Ethics Committee, 18 February 2021 (Ethics ref: 21/WM/0019). REC approval for the protocol version 2.0 dated 2 February 2021 was issued on 18 February 2021.We will present the findings in national and international conferences. We will also aim to publish the findings in high impact peer-reviewed journals. We will disseminate the completed paper to the Department of Health, the Scientific Advisory Committees of the RCOG, the Royal College of Nurses (RCN) and the BSGE.Trial registration number ISRCTN14566195.

Abstract Cognitive impairment is a term that refers to the impairment of one or more cognitive domains to varying degrees caused by a variety of reasons. It is under a high prevalence, many risk factors, complex etiology, and great harm to the elderly population. Early screening, diagnosis, and intervention for cognitive impairment in the elderly are of great importance. However, at present, the recognition rate of cognitive impairment for the elderly in China is low, the rate of missed diagnosis is high, and the evaluation is not standardized. This consensus integrates the commonly used cognitive function assessment scales in China and abroad, and aims to popularize the screening of cognitive impairment, standardize the evaluation methods and procedures of cognitive impairment in the elderly, and establish clinical diagnoses, interventions, and follow‐up plans in a timely manner.

This research was conducted at 4th Semester Students of English Language Education Study Program of Sembilanbelas November University Kolaka. The objectives of this research were to find out the translation methods that used to translate a report text by 4th semester students and the most dominant translation method used to translate a report text by 4th semester students. Kinds of translation method in this research were word for word translation, literal translation, and free translation. The most dominant method used to translate a report text in this research was Literal Translation. The design of this research was a qualitative content analysis. The subject was 4th Semester Students of English Language Education Study Program of Sembilanbelas November University Kolaka. The data collection was conducted through documentation. The data of this research were taken from the studentsâ translation work. The instrument of this research was translation test. The data of studentsâ translation work were analyzed using content analysis. The findings showed that there were 435 sentences of studentsâ translation work which divided into three kinds of translation methods according to Newmarkâs theory, namely word for word translation had 60 data with the percentage 14%. Literal translation had 304 data with the percentage 70%. The last free translation had 71 data with the percentage 16%. It showed that the most dominant translation method which was used by the students was literal translation that there were 304 or 70% data from 435 data of studentsâ translation work. Keywords: translation; translation method; and report text. Â

The pandemic of Covid-19 bring poor influence in various aspects such as the economy, health and finance. Increased problems and stress have an impact on domestic violence against women and children. It was reported the incidence of domestic violence increased during the pandemic as a result of stresses and uncertainty condition, especially when there is an imbalance of authority between men and women at home. There were 80% of female respondents in the middle to lower economic level reported receiving psychological and economic violence, and the implementation of Community Activity Restrictions (PPKM) in DKI Jakarta forced victims to live in the same house as the perpetrators of violence. For this reason, a program for an anti-violence campaign against women and children is needed with the aim to increase the awareness of women/wives due to domestic violence impact both for themselves, children and families. This program is carried out by academics through some events such as webinars, sharing messages through social media and launching the SAPA Pos (Friends of Women and Children) as a service forum for residents who need services and support. With a good understanding of violence, it is expected that DKI Jakarta residents will be more concerned in preventing violence experienced by women and children and have the courage to speak up/report it.

Fenomena pernikahan dini meningkatkan risiko masalah kesehatan gangguan psikologis anak, gangguan reproduksi, kanker serviks, penyakit menular seksual dan memicu stunting serta pengabaian pada anak yang dilahirkan. Kader dalam hal ini memegang peranan penting sebagai ujung tombak promosi kesehatan pada masyarakat. Penelitian ini dilakukan untuk menggali persepsi kader terhadap fenomena pernikahan dini pada remaja di wilayah Kabupaten Bandung Barat. Penelitian ini menggunakan metode penelitian kualitatif dengan pendekatan analisis konten. Teknik convenience sampling digunakan untuk proses seleksi partisipan. Hasil penelitian didapatkan 4 tema yaitu : sosial media dan pornografi, kondisi ekonomi keluarga, pola pikir orangtua, dan penggunaan teknologi untuk aktifitas positif pada remaja. Hasil penelitian ini menyarankan bahwa perawat komunitas perlu melakukan promosi kesehatan terkait pernikahan dini dengan cara yang inovatif bagi remaja

White shrimp, Penaeus vannamei, farming represents one of the most important aquaculture activities in the world with a high growth rate. However, intensification processes induce negative side effects on the health of the organism, associated with a dysbiosis phenomenon. Consequently, illnesses, mainly attributable to Vibrio genus bacteria, have been reported in shrimp ponds. Studying the diversity and ecology of the associated bacteria in aquaculture systems is essential to prevent and control diseases. Therefore, the present study analyzes the bacterial load and microbial population variation in P. vannamei hepatopancreases infected with a pathogenic Vibrio parahaemolyticus strain (so-called CVP2) associated with acute hepatopancreatic necrosis disease (AHPND) under controlled conditions. The results showed an important change in the microbial community structure of the P. vannamei hepatopancreas. Furthermore, the presence of the Vibrio genus considerably increased and clearly dominated compared with the control. Dysbiosis of the hepatopancreatic microbiota and constrictions in the hepatopancreatic tubules (characteristic signs of in the early stage of AHPND) could be observed before the visible manifestation of the disease.

Social media has been used as a marketing tool by architecture firms to generate purchase in­ten­tion. However, inspiration as a motivating internal response is needed to achieve the marketing ob­jec­tive. This study aims to examine the effect of utilitarian content, hedonic content, and openness as ante­cedents of customer inspiration, the effect of customer inspiration on purchase intention, and the me­diating role of customer inspiration between antecedents and purchase intention. A total of 395 res­pondents were taken as samples using simple random sampling, and the data was then analyzed with SmartPLS. Results show that utilitarian content, hedonic content, and openness positively affect cus­tomer inspiration, and customer inspiration positively affects purchase intentions. Additionally, cus­tomer inspiration also mediates the effect of utilitarian content, hedonic content, and openness on pur­chase intentions. This study contributes to the literature on customer inspiration from a customer-firm relationship perspective. Moreover, this study can give insight for architectural firms to effectively prac­tice social media marketing by focusing on creating customer inspiration through the content provided and targeting the right potential customers in order to generate architectural design service purchase intention.

The aim of this study was to investigate the influence of crossbreeding and the LPL genotype on goat milk yield, composition and quality indicators. This research was carried out in a herd of pure-breed Saanen, Anglo-Nubian, and crossbred Saanen and Anglo-Nubian goats (n=137) in Lithuania. Saanen x Anglo-Nubian crossbred goats and Saanen had a significantly (P

The authors report here the structure-function analysis of highly divergent actin from Leishmania parasite. The study reveals remarkably rapid dynamics of parasite actin as well as the underlying molecular basis, thus providing insight into evolution of the actin cytoskeleton.

Midazolam (MDZ) is used for sedation in surgical procedures; its clinical effect is related to its receptor affinity and the dose administered. Therefore, a pharmacokinetic–pharmacodynamic (PK-PD) population model of MDZ in pediatric patients undergoing minor surgery is proposed. A descriptive, observational, prospective, and longitudinal, study that included patients of both sexes, aged 2–17 years, ASA I/II, who received MDZ in IV doses (0.05 mg/kg) before surgery. Three blood samples were randomly taken between 5–120 min; both sedation by the Bispectral Index Scale (BIS) and its adverse effects were recorded. The PK-PD relationship was determined using a nonlinear mixed-effects, bicompartmental first-order elimination model using Monolix Suite™. Concentrations and the BIS were fitted to the sigmoid Emax PK-PD population and sigmoid Emax PK/PD indirect binding models, obtaining drug concentrations at the effect site (biophase). The relationship of concentrations and BIS showed a clockwise hysteresis loop, probably indicating time-dependent protein binding. Of note, at half the dose used in pediatric patients, adequate sedation without adverse effects was demonstrated. Further PK-PD studies are needed to optimize dosing schedules and avoid overdosing or possible adverse effects.

L-asparaginases from bacterial sources have been used in antineoplastic treatments and the food industry. A type II L-asparaginase encoded by the N-truncated gene ansZP21 of halotolerant Bacillus subtilis CH11 isolated from Chilca salterns in Peru was expressed using a heterologous system in Escherichia coli BL21 (DE3)pLysS. The recombinant protein was purified using one-step nickel affinity chromatography and exhibited an activity of 234.38 U mg−1 and a maximum catalytic activity at pH 9.0 and 60 °C. The enzyme showed a homotetrameric form with an estimated molecular weight of 155 kDa through gel filtration chromatography. The enzyme half-life at 60 °C was 3 h 48 min, and L-asparaginase retained 50% of its initial activity for 24 h at 37 °C. The activity was considerably enhanced by KCl, CaCl2, MgCl2, mercaptoethanol, and DL-dithiothreitol (p-value < 0.01). Moreover, the Vmax and Km were 145.2 µmol mL−1 min−1 and 4.75 mM, respectively. These findings evidence a promising novel type II L-asparaginase for future industrial applications.

The aim of this research was to determine the impact of pectoralis major myopathies on the physical parameters, fatty acid (FA) profile, and volatile organic compound (VOC) composition of chicken breast meat. Samples were collected from pectoralis major of broilers with varying severity scores (normal, mild, and severe) of wooden breast (WB) and white striping (WS) myopathies. Chicken breast meat affected by severe myopathies expressed higher cooking loss, drip loss (p < 0.001), and yellowness (p < 0.05) compared to those of samples that were taken from broilers without myopathies (normal). The amount of monounsaturated fatty acids (MUFAs) was significantly higher in samples affected by mild and severe myopathies than in those without myopathies (p < 0.05). There was significantly more aldehyde hexanal in muscles affected by mild and severe myopathies than in muscles without myopathies (p < 0.05). In conclusion, WB and WS myopathies of the breast muscle not only affected the physical parameters of broiler meat but also may have influenced its FA profile and VOC composition. Additionally, an elevated amount of hexanal in muscles affected by WB together with WS suggests that oxidative stress could be important in the etiopathogenesis of WB and WS myopathies. Therefore, poultry meat affected by myopathies have the potential to alter breast meat flavor and composition.

Pre-vaccination SARS-CoV-2 infection can boost protection elicited by COVID-19 vaccination and post-vaccination breakthrough SARS-CoV-2 infection can boost existing immunity conferred by COVID-19 vaccination. Such ‘hybrid immunity’ is effective against SARS-CoV-2 variants. In order to understand ‘hybrid immunity’ at the molecular level we studied the complementarity determining regions (CDR) of anti-RBD (receptor binding domain) antibodies isolated from individuals with ‘hybrid immunity’ as well as from ‘naive’ (not SARS-CoV-2 infected) vaccinated individuals. CDR analysis was done by liquid chromatography/mass spectrometry-mass spectrometry. Principal component analysis and partial least square differential analysis showed that COVID-19 vaccinated people share CDR profiles and that pre-vaccination SARS-CoV-2 infection or breakthrough infection further shape the CDR profile, with a CDR profile in hybrid immunity that clustered away from the CDR profile in vaccinated people without infection. Thus, our results show a CDR profile in hybrid immunity that is distinct from the vaccination-induced CDR profile.

BackgroundChocolate, as a cocoa-derived product rich in flavanols, has been used for medical and anti-inflammatory purposes. Therefore, the aim of this study was to investigate if the ingestion of different percentages of cocoa products affects the experimentally induced pain caused by intramuscular hypertonic saline injections in the masseter muscle of healthy men and women.MethodsThis experimental randomized, double-blind, and controlled study included 15 young, healthy, and pain-free men and 15 age-matched women and involved three visits with at least a 1-week washout. Pain was induced twice at each visit with intramuscular injections of 0.2 mL hypertonic saline (5%), before and after intake of one of the different chocolate types: white (30% cocoa content), milk (34% cocoa content), and dark (70% cocoa content). Pain duration, pain area, peak pain, and pressure pain threshold (PPT) were assessed every fifth minute after each injection, up until 30 min after the initial injection. Descriptive and inferential statistics were performed using IBM® SPSS (Version 27); significance level was set to pResultsThis study showed that intake of chocolate, no matter the type, reduced the induced pain intensity significantly more than no intake of chocolate (pConclusionIntake of chocolate before a painful stimulus had a pain-reducing effect no matter the cocoa concentration. The results indicate that perhaps it is not the cocoa concentration (e.g., flavanols) alone that explains the positive effect on pain, but likely a combination of preference and taste-experience. Another possible explanation could be the composition of the chocolate, i.e. the concentration of the other ingredients such as sugar, soy, and vanilla. ClinicalTrials.gov Identifier: NCT05378984.

Perencanaan keuangan merupakan hal penting yang harus dilakukan oleh setiap orang agar uang yang diperoleh dapat dimanfaatkan secara maksimal untuk meningkatkan kesejahteraan hidup, tanpa adanya perencanaan keuangan yang baik maka tidak menutup kemungkinan akan terjadi pengeluaran yang lebih besar daripada pemasukkan. Di Indonesia ternyata baru 12,6 % masyarakat yang telah melakukan perencanaan keuangan. Oleh karena itu sangat penting untuk memperoleh pengetahuan mengenai perencanaan keuangan yang mempelajari cara mengelola utang, investasi dan mempersiapkan pensiun melalui suatu kegiatan pengabdian kepada masyarakat. Tujuan kegiatan pengabdian masyarakat ini adalah untuk memberikan pengetahuan kepada karyawan PT Atrya Swascipta Rekayasa mengenai perencanaan keuangan, cara mengelola utang dan persiapan pensiun agar dapat meningkatkan kesejahteraan karyawan PT Atrya Swascipta Rekayasa. Metode yang digunakan dalam kegiatan pengabdian masyarakat di PT Atrya Swascipta Rekayasa adalah mengadakan talkshow singkat dan workshop mengenai perencanaan keuangan, mengelola utang dan persiapan pensiun. Hasil yang diharapkan dari kegiatan pengabdian kepada masyarakat ini adalah karyawan PT Atrya Swascipta Rekayasa dapat memperoleh pengetahuan dan berusaha menerapkan pengetahuan mengenai perencanaan keuangan, cara mengelola utang dan persiapan pensiun sehingga kesejahteraan karyawan tersebut dapat meningkat dari waktu ke waktu.

Penelitian ini bertujuan menyelidiki perbedaan hasil belajar matematika dalam pembelajaran dengan metode Inquiry dan Ekspositori siswa kelas X SMKN 12 Samarinda tahun pelajaran 2019/2020. Penelitian eksperimen semu ini menggunakan Nonequivalent Group Pretest Posttest Design. Populasi penelitian mencakup semua siswa kelas X SMKN 12 Samarinda. Sampel penelitian ditentukan dengan teknik Purposive Sampling dan diperoleh dua kelas, yaitu X TKJ & X AK1 SMKN 12 Samarinda sebagai sampel penelitian. Pengambilan data menggunakan tiga instrumen, yaitu tes, observasi, dan dokumentasi. Sebelum untuk pengambilan data, soal tes diujicobakan kepada 32 siswa kelas X jurusan TKJ SMK Muhammadiyah di Loa Janan. Analisi data penelitian menggunakan teknik Independent Sample t-test pada α=0,05. Hasil penelitian menunjukkan rata-rata skor hasil belajar matematika siswa kelompok eksperimen (63,12) lebih tinggi daripada siswa kelompok kontrol (50,84). Hasil uji t menunjukkan nilai Sig.=0.04 dan tenyata lebih kecil dari taraf α=0.05 sehingga H0 ditolak. Dengan demikian, terdapat perbedaan hasil belajar matematika siswa dalam pembelajaran menggunakan metode Inquiry dan Ekspositori kelas X SMKN 12 Samarinda tahun pelajaran 2019/2020. Berdasarkan Uji Satu Pihak (uji pihak kanan) diperoleh Sig.=0.004 sehingga H0 ditolak, berarti pembelajaran yang menggunakan metode Inquiry menghasilkan hasil belajar yang lebih tinggi dan signifikan daripada menggunakan metode Ekspositori.

Three burdens of malnutrition, namely undernutrition, overnutrition, and lack of macronutrients are problems faced by adolescents in Indonesia. Nutritional problems related to development and growth are often experienced by adolescents because the adolescent's body requires more energy and nutrients than children. Previous studies on nutritional intake and nutritional status in adolescents reported inconsistent results. This study aims to determine the relationship between nutritional intake and nutritional status in adolescent girls in Buleleng Bali. This study uses a cross-sectional analytic observational design conducted in two high schools in Buleleng Regency with 163 respondents. The instruments used in this study were a questionnaire, 2x24 hour food recall, weight measurement with a weight scale, height measurement with a mechanical meter, and the World Health Organization (WHO) AnthroPlus software to calculate the body mass index by age (z-score). Spearman correlation test was performed for bivariate analysis and multiple linear regression for multivariate analysis. Bivariate analysis showed that the variables of fat intake (p-value=0,001) and total energy intake (p-value=0,008) showed a significant correlation with the z-score. In multivariate analysis, the variable that showed a significant relationship with the z-score was fat intake (β=0,023, 95%CI=0,013-0,033, p-value=0,001). Nutritional intake that has a significant relationship with nutritional status in adolescent girls in Buleleng Regency is fat intake. Limitation of fat consumption needs to be done.

Knowledge is the most important strategic resource for knowledge-based organizations such as higher education to achieve sustainable competitive advantage. Facing dynamic and unpredictable competition, organizations not only rely solely on a workforce training system that focuses on selecting workers who have specific knowledge, skills, abilities, or competencies, but also organizations must start thinking about how the knowledge sharing process can be carried out in organization. This study is conducted to investigate factors influencing knowledge sharing activities among academics in Indonesia. These factors include individual factors, group factors, organizational factors, and technological factors. This study involved academics from public and private universities in Indonesia and data collected through surveys. This study involved 141 respondents from 17 study programs which include 31 public and private universities in Indonesia. The results showed that individual factors, organizational factors, and technological factors had a significant influence on knowledge sharing activities among academics in higher education, while group factors did not have a significant effect on knowledge sharing activities between academics in universities in Indonesia