80 results on '"Lin-Zi, Li"'
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2. The anti-hyperlipidemia effect of Atractylodes macrocephala Rhizome increased HDL via reverse cholesterol transfer
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Bo Li, Xian-fang Chen, Han-song Wu, Jie Su, Yan-yan Ding, Ze-hua Zhang, Mei Rong, Ying-jie Dong, Xinglishang He, Lin-zi Li, Gui-yuan Lv, and Su-hong Chen
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Hyperlipidemia ,HDL ,Atractylodes macrocephala Rhizome (AM) ,Network pharmacology ,Reverse cholesterol transport (RCT) ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Aim: Atractylodes macrocephala Rhizome (AM) has been used to treat hyperlipidemia for centuries, but its functional components and mechanisms are not clear. This research aimed to investigate the active components in AM and the mechanisms that underlie its anti-hyperlipidemia effect. Methods: SD rats were fed a high-sucrose high-fat diet in conjunction with alcohol (HSHFDAC) along with different AM extracts (AMW, AMO, AME, and AMP) for 4 weeks. AM's active components were analyzed using multiple databases, and their mechanisms were explored through network pharmacology. The relationship between AM's effect of enhancing serum HDL-c and regulating the expression of reverse cholesterol transport (RCT)-related proteins (Apo-A1, LCAT, and SR-BI) was further validated in the HSHFDAC-induced hyperlipidemic rats. The kidney and liver functions of the rats were measured to evaluate the safety of AM. Results: AMO, mainly comprised of volatile and liposoluble components, contributed the most significant anti-hyperlipidemia effect among the four extracts obtained from AM, significantly improving the blood lipid profile. Network pharmacology analysis also suggested that volatile and liposoluble components, comprise AM's main active components and they might act on signaling pathways associated with elevated HDL-c. Validation experiments found that AMO substantially and dose-dependently increased HDL-c levels, upregulated the expression of Apo-A1, SR-BI, and LCAT, improved the pathological changes in the kidney and liver, and significantly reduced the serum creatinine levels in rats with hyperlipidemia. Conclusion: The main anti-hyperlipidemia active components of AM are its volatile and liposoluble components, which may enhance serum HDL-c by increasing the expression of the RCT-related proteins Apo-A1, LCAT, and SR-BI.
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- 2024
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3. Structural identification of an polysaccharide isolated from Epimedium brevicornum and its beneficial effect on promoting osteogenesis in osteoblasts induced by high glucose
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Shan shan Lei, Bo Li, Xiao wen Huang, Xu pin Wang, Shan Xiong, Rui Duan, and Lin zi Li
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Diabetes osteoporosis ,Epimedium brevicornum polysaccharide ,Structural resolution ,Pharmacological mechanisms ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Aim: Diabetes osteoporosis (DOP) is a chronic bone metabolic disease induced by diabetes, whose morbidity continues to increase. Epimedium brevicornum Maxim (EB), a popular Chinese traditional medicine, has been used to treat bone diseases in China for thousands of years. But its material basis and specific mechanism of action are not clear. Methods: Epimedium brevicornum crude polysaccharide (EPE) is the main component, in this research the characterized the structure of EBPC1 purified from EPE was detected and its effects on cell proliferation, differentiation, and cytoskeletal in osteoblasts induced by high glucose. Results: The molecular weight of EBPC1 was 10.5 kDa. It was mainly comprised of glucose and galactose, and the backbone of EBPC1 was→4)-α-D-Galp-(1→4)-α-D-Galp-(1→6)-β-D-Galp-(1→6)-β-D-Galp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→. The results from in vitro experiments revealed that EBPC1 significantly increased alkaline phosphatase (ALP) activity and mineralized nodule formation in primary osteoblasts, also significantly up-regulated expression of Alp mRNA and Runx2 mRNA in the presence of EBPC1 pretreatment. Moreover, EBPC1 modulated apoptosis via the regulation of Bax/Bcl2. Conclusion: These results indicate that EBPC1 treatment can promote osteogenesis during DOP, which can ameliorate apoptosis by regulating Bax/Bcl2 and accelerating osteogenesis in osteoblasts.
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- 2023
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4. Beneficial effects of Hawthorn extract on metabolic hypertensive rats by regulating Treg/Th17 immune balance
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Bing-Bing Cheng, Ze-Qi Hu, Bo Li, Xiang Zheng, Lin-Zi Li, Ying-Jie Dong, Xing-Li-Shang He, Gui-Yuan Lv, Su-Hong Chen, and Ning-Hua Jiang
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Hawthorn extract ,Metabolic hypertension ,Metabolic abnormalities ,Tregs/Th17 ,Inflammation ,Nutrition. Foods and food supply ,TX341-641 - Abstract
There is an increasing global trend toward unhealthy lifestyles and dietary decisions, like the overconsumption of alcohol and high-sugar and high-fat diets (ACHSFDs). Poor eating habits can cause an imbalance of the Treg/Th17 immune axis, thereby leading to an imbalance of anti-inflammatory and pro-inflammatory factors, which damages target organs and the vascular endothelium causing MH. Hawthorn has been shown to improve gastrointestinal function, lower blood pressure, and regulate metabolic abnormalities. However, whether it acts on MH by regulating the Treg/Th17 immune axis remains unclear. In this study, the hawthorn extract reduced blood pressure[systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean blood pressure (MBP)] and improved dyslipidemia in rats with ACHSFD-induced MH. In addition, the extract reduced serum AST and ALT levels and liver coefficient and improved liver fat deposition and fibrosis. On the other hand, the hawthorn extract downregulated RORγt expression, upregulated Foxp3 expression, increased IL-10 levels in the aorta, and reduced IL-17, IL-6, and TNF-α content. Moreover, the extract upregulated eNOS expression in the aorta and increased NO content in the serum. These findings indicate that hawthorn extract can inhibit aortic inflammatory injury and improve vascular endothelial function by regulating the Treg/Th17 immune imbalance.
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- 2023
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5. Polysaccharide, the Active Component of Dendrobium officinale, Ameliorates Metabolic Hypertension in Rats via Regulating Intestinal Flora-SCFAs-Vascular Axis
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Bo Li, Hui-Ying Wang, Jia-Hui Huang, Wan-Feng Xu, Xiao-Jie Feng, Ze-Ping Xiong, Ying-Jie Dong, Lin-Zi Li, Xinglishang He, Han-Song Wu, Ke Zhang, Jie Su, Qiao-Xian Yu, Ning-Hua Jiang, Gui-Yuan Lv, and Su-Hong Chen
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Dendrobium officinale polysaccharide ,metabolic hypertension ,short chain fatty acids ,G protein-coupled receptor ,strengthened enterogastric function ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Metabolic hypertension (MH) is the most common type of hypertension worldwide because of unhealthy lifestyles, such as excessive alcohol intake and high-sugar/high-fat diets (ACHSFDs), adopted by humans. Poor diets lead to a decrease in the synthesis of short-chain fatty acids (SCFAs), which are produced by intestinal flora and transferred by G protein-coupled receptors (GPCRs), resulting in impaired gastrointestinal function, disrupted metabolic processes, increased blood pressure (BP), and ultimately, MH. It is not clear whether Dendrobium officinale polysaccharide (DOPS) can mediate its effects by triggering the SCFAs-GPCR43/41 pathway. In this study, DOPS, with a content of 54.45 ± 4.23% and composition of mannose, glucose, and galacturonic acid at mass percentages of 61.28, 31.87, and 2.53%, was isolated from Dendrobium officinale. It was observed that DOPS, given to rats by intragastric administration after dissolution, could lower the BP and improve the abnormal lipid metabolic processes in ACHSFD-induced MH rats. Moreover, DOPS was found to increase the production, transportation, and utilization of SCFAs, while improving the intestinal flora and strengthening the intestinal barrier, as well as increasing the intestinal levels of SCFAs and the expression of GPCR43/41. Furthermore, DOPS improved vascular endothelial function by increasing the expression of GPCR41 and endothelial nitric oxide synthase in the aorta and the nitric oxide level in the serum. However, these effects were all reversed by antibiotic use. These findings indicate that DOPS is the active component of Dendrobium officinale, and it can reverse MH in rats by activating the intestinal SCFAs-GPCR43/41 pathway.
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- 2022
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6. Modulating effects of a functional food containing Dendrobium officinale on immune response and gut microbiota in mice treated with cyclophosphamide
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Ying-Jie Dong, Min-Qiu Lin, Xi Fang, Zhi-Yi Xie, Rong Luo, Xi Teng, Bin Li, Bo Li, Lin-Zi Li, Hai-Ying Jin, Qiao-Xian Yu, Gui-Yuan Lv, and Su-Hong Chen
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National Herbal Drink (NHD) ,Cyclophosphamide (CTX) ,Immunity ,Gut microbiota ,Nutrition. Foods and food supply ,TX341-641 - Abstract
National Herbal Drink (NHD) is a functional food approved by the State Administration for Market Regulation for immune enhancement. In this study, the effects of NHD on Cyclophosphamide (CTX)-induced immune regulation and gut microbiota dysregulation in mice were investigated to elucidate whether the attenuation of immunosuppression was related to the regulation of the gut microbiota by NHD. Our study showed that CTX induced immune organ damage in immunosuppressed mice, with reduced levels of serum immunoglobulins IgG, IgM, and complement C3 and disruption of bone marrow hematopoiesis, accompanied by changes in liver toxicity and shortened colonic length. However, all these changes were reversed to varying degrees by NHD intervention. Delayed-type hypersensitivity (DTH) and serum hemolysins in mice also indicated that NHD could effectively regulate cellular and humoral immunity in the body. In addition, it is noteworthy that NHD can also improve intestinal pathology, elevate the expression of intestinal tight junction proteins Occludin and Claudin-1 to restore intestinal barrier function. Furthermore, α-diversity and β-diversity analysis showed that NHD has a positive regulatory effect on the gut microbiota. At the phylum level, NHD could regulate the abundance of three major phyla, namely, Bacteroidetes, Firmicutes, and Actinobacteriota. At the species level, the abundance of the beneficial genus uncultured_bacterium_g__norank_f__ Muribaculaceae increased and the abundance of the harmful genus unclassified_f__Lachnospiraceae decreased after the NHD intervention. These results provide evidence that NHD modulates the immune response in cyclophosphamide-induced immunocompromised mice by restoring intestinal barrier function and regulating gut microbiota disorders.
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- 2022
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7. Soporific effect of modified Suanzaoren Decoction on mice models of insomnia by regulating Orexin-A and HPA axis homeostasis
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Ying-Jie Dong, Ning-Hua Jiang, Liang-Hui Zhan, Xi Teng, Xi Fang, Min-Qiu Lin, Zhi-Yi Xie, Rong Luo, Lin-Zi Li, Bo Li, Bei-Bei Zhang, Gui-Yuan Lv, and Su-Hong Chen
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Modified Suanzaoren Decoction (MSZRD) ,Insomnia ,5-HT ,OX2R ,Orexin-A ,HPA axis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Aim: Modified Suanzaoren Decoction (MSZRD) is obtained by improving Suanzaoren Decoction (SZRT), a traditional Chinese herbal prescription that has been used to treat insomnia for more than thousands of years. Our previous study showed that MSZRD can improve the gastrointestinal discomfort related insomnia by regulating Orexin-A. This study is the first study to evaluate the effects and possible mechanisms of MSZRD in mice with insomnia caused by p-chlorophenylalanine (PCPA) combined with multifactor random stimulation. Methods: After 14 days of multifactor stimulation to ICR mice, a PCPA suspension (30 mg/mL) was injected intraperitoneally for two consecutive days to establish an insomnia model. Three different doses of MSZRD (3.6, 7.2, and 14.4 g/kg/day) were given to ICR mice for 24 days. The food intake and back temperature were measured, and behavioral tests and pentobarbital sodium-induced sleep tests were conducted. The levels of Orexin-A, corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), and adrenocortical hormones (CORT) in the serum and 5-hydroxytryptamine (5-HT), dopamine (DA), and norepinephrine (NE) in hypothalamus were measured using enzyme-linked immunosorbent assay (ELISA) kits. The levels of γ-aminobutyric acid (GABA) and glutamic acid (Glu) were measured by high-performance liquid chromatography (HPLC). The expression of 5HT1A receptor (5-HTRIA) and orexin receptor 2 antibody (OX2R) was measured by Western blot (WB) and immunohistochemical staining (ICH). Hematoxylin and eosin (H&E) staining and Nissl staining were used to assess the histological changes in hypothalamus tissue. Results: Of note, MSZRD can shorten the sleep latency of insomnia mice (P
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- 2021
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8. Pitongshu Alleviates the Adverse Symptoms in Rats with Functional Dyspepsia Through Regulating Visceral Hypersensitivity Caused by 5-HT Overexpression
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Kun-Gen Wang, Gui-Yuan Lv, Shu-Hua Shen, Su-Hong Chen, Li-Jie Zhu, Yi-Hui Zhi, Han-Song Wu, Lin-Zi Li, and Bo Li
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Organic Chemistry ,Drug Discovery ,General Medicine ,Computer Science Applications - Abstract
Aim: The aim of the study was to explore the efficacy as well as the mechanism of action of Pitongshu (PTS) on rats with functional dyspepsia (FD) induced by iodoacetamide gavage and tail clamping. Methods: The bioactive components of PTS were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), whereas the potential targets of PTS were obtained from the Similarity Ensemble Approach (SEA), TCMSP, and Swiss Target Prediction Database. The disease targets were obtained from the DisGeNET database, whereas Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed using the R Software. The method of iodoacetamide gavage combined with tail clamping was used to establish the FD rat model in this study. Body weight, food intake, gastrointestinal motility, gastric acidity and secretion, and the mechanical pain threshold of rats were measured. The open-field test was also performed. The stomach and duodenum were histologically observed. The levels of serotonin (5-HT), Calcitonin Gene-Related Peptide (CGRP), Motilin (MTL), and Gastrin (GAS) in gastric tissues were detected by ELISA. Results: A total of 139 bioactive components and 17 potential targets of PTS were identified through a network pharmacology approach. The results of GO and KEGG enrichment analyses indicated that PTS could reduce the 5-HT secretion of gastric tissues through the serotonergic synaptic pathway and alleviate the symptoms of FD, indicating that PTS plays a therapeutic role. The results of animal experiments showed that PTS could increase body weight and food intake, improve autonomous activity, and decrease gastric acidity and secretion in FD rats. Furthermore, gastric sensitivity increased in FD rats, and PTS treatment could significantly decrease it. The results of ELISA showed that the overexpression of 5-HT and CGRP was decreased after PTS treatment in FD rats. Lastly, PTS could significantly improve gastrointestinal motility, as well as the levels of GAS and MTL in FD rats. Conclusion: PTS may reduce 5-HT secretion by regulating the serotonergic synaptic pathway, thereby reducing visceral sensitivity and alleviating the symptoms of FD.
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- 2023
9. Effects of a Macroporous Resin Extract of Dendrobium officinale Leaves in Rats with Hyperuricemia Induced by Anthropomorphic Unhealthy Lifestyle
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Lin-Zi Li, Xi-Ming Wang, Xiao-Jie Feng, Kun Liu, Bo Li, Li-Jie Zhu, Wan-Feng Xu, Xiang Zheng, Ying-Jie Dong, Xing-Lishang He, Hao-Ru Guan, Yan-Yan Ding, Han-Song Wu, Chuan-Jie Zhou, Sen-Yu Ye, Bei-Bei Zhang, Gui-Yuan Lv, and Su-Hong Chen
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Article Subject ,Complementary and alternative medicine - Abstract
Aim. Hyperuricemia (HUA) has received increased attention in the last few decades due to its global prevalence. Our previous study found that administration of a macroporous resin extract of Dendrobium officinale leaves (DoMRE) to rats with HUA that was induced by exposure to potassium oxazine combined with fructose and a high-purine diet led to a significant reduction in serum uric acid (SUA) levels. The aim of this study was to explore the effects of DoMRE on hyperuricemia induced by anthropomorphic unhealthy lifestyle and to elucidate its possible mechanisms of action. Methods. Dosages (5.0 and 10.0 g/kg/day) of DoMRE were administered to rats daily after induction of HUA by anthropomorphic unhealthy lifestyle for 12 weeks. The levels of UA in the serum, urine, and feces; the levels of creatinine (Cr) in the serum and urine; and the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum were all measured using an automatic biochemical analyzer. The activities of xanthine oxidase (XOD) and adenosine deaminase (ADA) in the serum, liver, and intestine tissue supernatant were measured using appropriate kits for each biological target. The expressions levels of UA transporters (ABCG2 and GLUT9), tight junction (TJ) proteins (ZO-1 and occludin), and inflammatory factors (IL-6, IL-8, and TNF-α) in the intestine were assayed by immunohistochemical (IHC) staining. Hematoxylin and eosin (H&E) staining was used to assess histological changes in the renal and intestinal tissues. Results. DoMRE treatment significantly reduced SUA levels and concomitantly increased fecal UA (FUA) levels and the fractional excretion of UA (FEUA) in HUA rats. Furthermore, DoMRE significantly reduced both the XOD activity in the serum, liver, and intestine and the ADA activity in the liver and intestine. DoMRE also effectively regulated the expression of GLUT9 and ABCG2 in the intestine, and it significantly upregulated the expression of the intestinal TJ proteins ZO-1 and occludin. Therefore, DoMRE reduced the damage to the intestinal barrier function caused by the increased production of inflammatory factors due to HUA to ensure normal intestinal UA excretion. Conclusion. DoMRE demonstrated anti-HUA effects in the HUA rat model induced by an anthropomorphic unhealthy lifestyle, and the molecular mechanism appeared to involve the regulation of urate transport-related transporters (ABCG2 and GLUT9) in the intestine, protection of the intestinal barrier function to promote UA excretion, and inhibition of XOD and ADA activity in the liver and intestine to inhibit UA production in the HUA-induced rats.
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- 2023
10. Hypertensive Rats Treated Chronically With Nω-Nitro-L-Arginine Methyl Ester (L-NAME) Induced Disorder of Hepatic Fatty Acid Metabolism and Intestinal Pathophysiology
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Bo Li, Xinglishang He, Shan-Shan Lei, Fu-Chen Zhou, Ning-Yu Zhang, Ye-Hui Chen, Yu-Zhi Wang, Jie Su, Jing-Jing Yu, Lin-Zi Li, Xiang Zheng, Rong Luo, Dorota Kołodyńska, Shan Xiong, Gui-Yuan Lv, and Su-Hong Chen
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hypertension ,liver injury ,multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) ,fatty acid ,intestinal pathophysiology ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Nω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) biosynthesis, results in hypertension and liver injury. This study aimed at investigating the changes of liver lipometabonomics and exploring the underlying mechanisms of liver injury in the L-NAME-treated rats. The male Sprague-Dawley (SD) rats were treated with L-NAME (40 mg/kg, p.o.) for 8 weeks. After that, the liver, aorta, fecal, and serum were collected for analysis. The results showed that L-NAME induced hypertension and disordered the endothelial nitric oxide synthase (eNOS)-NO pathway in the treated rats. L-NAME could also increase the levels of serum total cholesterol (TC), triglyceride (TG), alanine transaminase (ALT), and aspartate transaminase (AST). The multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) analysis showed that L-NAME could induce significant changes of the total hepatic lipids and most hepatic triglycerides, as well as fatty acid (FA). A positive correlation was found between the blood pressure and TAG. Immunofluorescence and Western-Blot experiments indicated that the L-NAME treatment significantly influenced some FA β-oxidation, desaturation, and synthesis-related proteins. The increase of intestinal inflammation, decrease of microcirculation and tight junction proteins, as well as alterations of microbial communities were observed in the L-NAME induced hypertensive rats, as well as alterations of microbial communities were notable correlation to TAG and FA species. This study demonstrated that the L-NAME-induced hypertensive rats exhibiting liver injury were the joint action of hepatic abnormal fatty acid metabolism and microcirculation disorder. Furthermore, the gut microflora, as well as the changes of FA β-oxidation (ACOX, CPT1α), desaturation (SCD-1), and synthesis (FAS) may be the potential mechanisms for abnormal fatty acid metabolism.
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- 2020
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11. Molecular mechanism of the effect of Gegen Qinlian decoction on COVID-19 comorbid with diabetes mellitus based on network pharmacology and molecular docking: A review.
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Lin-zi Li, Cong Zhou, Pei Wang, Qing-hua Ke, Jie Zhang, Shan-shan Lei, and Zhi-qiang Li
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- 2023
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12. A specific inhibitor of CDK1, RO-3306, reversibly arrests meiosis during in vitro maturation of porcine oocytes
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Jang, Woo-In, Lin, Zi-Li, Lee, Sung Hyun, Namgoong, Suk, and Kim, Nam-Hyung
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- 2014
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13. Distinct subcellular localization and potential role of LINE1-ORF1P in meiotic oocytes
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Luo, Yi-Bo, Zhang, Li, Lin, Zi-Li, Ma, Jun-Yu, Jia, Jialin, Namgoong, Suk, and Sun, Qing-Yuan
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- 2016
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14. Beneficial effects ofDendrobium officinaleon metabolic hypertensive rats by triggering the enteric-origin SCFA-GPCR43/41 pathway
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Qiao-Xian Yu, Jie Su, Ning-Yu Zhang, Rong Luo, Ning-Hua Jiang, Bo Li, Dong-Ying Jie, Fu-Chen Zhou, Xinglishang He, Su-Hong Chen, Hai-Ying Jin, Xiang Zheng, Gui-Yuan Lv, Lin-Zi Li, and Hui-Ying Wang
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Male ,0301 basic medicine ,medicine.medical_specialty ,Nitric Oxide Synthase Type III ,Blood Pressure ,030204 cardiovascular system & hematology ,Nitric Oxide ,Receptors, G-Protein-Coupled ,Nitric oxide ,Feces ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Enos ,Internal medicine ,medicine ,Animals ,Receptor ,chemistry.chemical_classification ,biology ,Chemistry ,Fatty acid ,General Medicine ,Metabolism ,Fatty Acids, Volatile ,biology.organism_classification ,Diet ,Gastrointestinal Microbiome ,Rats ,Gastrointestinal Tract ,Disease Models, Animal ,Cholesterol ,030104 developmental biology ,Endocrinology ,Blood pressure ,Liver ,Dietary Supplements ,Hypertension ,Dendrobium ,Gastrointestinal function ,Signal Transduction ,Food Science - Abstract
Given the increasing global trend toward unhealthy lifestyles and dietary decisions, such as "over-consumption of alcohol, and high sugar and fat diets" (ACHSFDs), it is not surprising that metabolic hypertension (MH) is now the most common type of hypertension. There is an urgent, global need for effective measures for the prevention and treatment of MH. Improper diet leads to decreased short-chain fatty acid (SCFA) production in the gut, leading to decreased gastrointestinal function, metabolism, and blood pressure as a result of signaling through G-protein-coupled receptors (GPCRs), ultimately causing MH. Previous studies have suggested that Dendrobium officinale (DO) may improve gastrointestinal function, lower blood pressure, and regulate metabolic abnormalities, but it is not clear whether it acts on MH by increasing SCFA and, if so, how. In this research, it was observed that Dendrobium officinale ultrafine powder (DOFP) could lower blood pressure and improve lipid abnormalities in ACHSFD-induced MH model rats. Moreover, DOFP was found to improve the intestinal flora and increased the SCFA level in feces and serum, as well as increased the expressions of GPCR43/41 and eNOS and the nitric oxide (NO) level. An experiment on isolated aorta rings revealed that DOFP improved the vascular endothelial relaxation function in MH rats, and this effect could be blocked by the eNOS inhibitor l-NAME. These experimental results suggest that DOFP improved the intestinal flora and increased the production, transportation, and utilization of SCFA, activated the intestinal-vascular axis SCFA-GPCR43/41 pathway, improved vascular endothelial function, and finally lowered blood pressure in MH model rats. This research provides a new focus for the mechanism of the effect of DOFP against MH by triggering the enteric-origin SCFA-GPCR43/41 pathway.
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- 2021
15. Spindlin 1 is essential for metaphase II stage maintenance and chromosomal stability in porcine oocytes
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Choi, Jeong-Woo, Zhao, Ming-Hui, Liang, Shuang, Guo, Jing, Lin, Zi-Li, Li, Ying-Hua, Jo, Yu-Jin, Kim, Nam-Hyung, and Cui, Xiang-Shun
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- 2017
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16. Dendrobium officinalis Flower Improves Learning and Reduces Memory Impairment by Mediating Antioxidant Effect and Balancing the Release of Neurotransmitters in Senescent Rats
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Su-Hong Chen, Lin-Zi Li, Bo Li, Fu-Chen Zhou, Shan-Shan Lei, Ye-Hui Chen, Jie Su, and Gui-Yuan Lv
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Male ,China ,Antioxidant ,medicine.medical_treatment ,Glutathione reductase ,Morris water navigation task ,Flowers ,Pharmacology ,medicine.disease_cause ,Hippocampus ,Antioxidants ,Rats, Sprague-Dawley ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Polysaccharides ,Drug Discovery ,medicine ,Animals ,Learning ,Hippocampus (mythology) ,030304 developmental biology ,Flavonoids ,Memory Disorders ,Neurotransmitter Agents ,0303 health sciences ,biology ,Organic Chemistry ,General Medicine ,Malondialdehyde ,Rats ,Computer Science Applications ,Oxidative Stress ,chemistry ,biology.protein ,Monoamine oxidase B ,Dendrobium ,030217 neurology & neurosurgery ,Oxidative stress - Abstract
Aim and Objective: The Dendrobium officinalis flower (DOF) is popular in China due to common belief in its anti-aging properties and positive effects on “nourish yin”. However, there have been relatively few confirmatory pharmacological experiments conducted to date. The aim of this work was to evaluate whether DOF has beneficial effects on learning and memory in senescent rats, and, if so, to determine its potential mechanism of effect. Materials and Methods: SD rats were administrated orally DOF at a dose of 1.38, or 0.46 g/kg once a day for 8 weeks. Two other groups included a healthy untreated control group and a senescent control group. During the 7th week, a Morris water maze test was performed to assess learning and memory. At the end of the experiment, serum and brain samples were collected to measure concentrations of antioxidant enzymes, including malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH-Px) in serum, and the neurotransmitters, including γ-aminobutyric acid (γ-GABA), Glutamic (Glu), and monoamine oxidase B (MAO-B) in the brain. Histopathology of the hippocampus was assessed using hematoxylin-eosin (H&E) staining. Results: The results suggested that treatment with DOF improved learning as measured by escape latency, total distance, and target quadrant time, and also increased levels of γ-GABA in the brain. In addition, DOF decreased the levels of MDA, Glu, and MAO-B, and improved SOD and GSHPx. Histopathological analysis showed that DOF also significantly reduced structural lesions and neurodegeneration in the hippocampus relative to untreated senescent rats. Conclusion: DOF alleviated brain aging and improved the spatial learning abilities in senescent rats, potentially by attenuating oxidative stress and thus reducing hippocampal damage and balancing the release of neurotransmitters.
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- 2020
17. The Mechanism of Dendrobium officinale as a Treatment for Hyperlipidemia Based on Network Pharmacology and Experimental Validation
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Lin-Zi Li, Hui-Ying Wang, Jia-Hui Huang, Kun Liu, Xiao-Jie Feng, Xi-Ming Wang, Li-Jie Zhu, Xing-Lishang He, Xiang Zheng, Hai-Long Li, Ying-Jie Dong, Bo Li, Han-Song Wu, Ning-Hua Jiang, Gui-Yuan Lv, and Su-Hong Chen
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Complementary and alternative medicine ,Article Subject ,nutritional and metabolic diseases - Abstract
Aim and Objective. Hyperlipidemia is a public health matter of global scale, contributing to a wide range of diseases that can result in severe complications and significant annual mortality. Dendrobium officinale (DO) is an edible plant with a long medicinal history in China. Our previous studies revealed that DO may have therapeutic benefits in lipid disorders. However, the mechanism of its active compounds is still unclear. This research aimed at uncovering the hidden anti-hyperlipidemia mechanisms of DO through network pharmacology and experimental validation. Materials and Methods. The active compounds in DO, their targets, and targets associated with hyperlipidemia were screened across various databases, and the hidden targets of DO in treating hyperlipidemia were forecast. The compound-target (C-T), protein-protein interaction (PPI), and compound-target-pathway (C-T-P) networks of DO were set up with Cytoscape software. The hub genes and core clusters of DO predicted to be active against hyperlipidemia were calculated by Cytoscape. The DAVID database was adopted for Gene Ontology (GO) analysis and KEGG pathway enrichment analysis. Next, we used the high-sucrose-fat diet and alcohol (HFDA)-induced hyperlipidemia rats to evaluate the hypolipidemic effect of DO. Results. In this study, we obtained 264 compounds from DO, revealed 11 bioactive compounds, and predicted 89 potential targets of DO. The network analysis uncovered that naringenin, isorhamnetin, and taxifolin might be the compounds in DO that are mainly in charge of its roles in hyperlipidemia and might play a role by modulating the targets (including PPARG, ADIPOQ, AKT1, TNF, and APOB). The pathway analysis showed that DO might affect diverse signaling pathways related to the pathogenesis of hyperlipidemia, including PPAR signaling pathway, insulin resistance, AMPK signaling pathway, and non-alcoholic fatty liver disease simultaneously. Meanwhile, in the HFDA-induced hyperlipidemia rat model, DO could significantly decrease the level of TC, TG, LDL-c, and ALT in serum, and increase HDL-c as well. The liver pathological section indicated that DO could ease liver damage and lipid cumulation. Conclusion. In summary, the biological targets of the main bioactive compounds in DO were found to distribute across multiple metabolic pathways. These findings suggest that a mutual regulatory system consisting of multiple components, targets, and pathways is a likely mechanism through which DO may improve hyperlipidemia. Validation experiments indicated that DO may treat hyperlipidemia by affecting NAFLD-related signaling pathways.
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- 2022
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18. The Mechanism of
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Lin-Zi, Li, Hui-Ying, Wang, Jia-Hui, Huang, Kun, Liu, Xiao-Jie, Feng, Xi-Ming, Wang, Li-Jie, Zhu, Xing-Lishang, He, Xiang, Zheng, Hai-Long, Li, Ying-Jie, Dong, Bo, Li, Han-Song, Wu, Ning-Hua, Jiang, Gui-Yuan, Lv, and Su-Hong, Chen
- Abstract
The active compounds in DO, their targets, and targets associated with hyperlipidemia were screened across various databases, and the hidden targets of DO in treating hyperlipidemia were forecast. The compound-target (C-T), protein-protein interaction (PPI), and compound-target-pathway (C-T-P) networks of DO were set up with Cytoscape software. The hub genes and core clusters of DO predicted to be active against hyperlipidemia were calculated by Cytoscape. The DAVID database was adopted for Gene Ontology (GO) analysis and KEGG pathway enrichment analysis. Next, we used the high-sucrose-fat diet and alcohol (HFDA)-induced hyperlipidemia rats to evaluate the hypolipidemic effect of DO.In this study, we obtained 264 compounds from DO, revealed 11 bioactive compounds, and predicted 89 potential targets of DO. The network analysis uncovered that naringenin, isorhamnetin, and taxifolin might be the compounds in DO that are mainly in charge of its roles in hyperlipidemia and might play a role by modulating the targets (including PPARG, ADIPOQ, AKT1, TNF, and APOB). The pathway analysis showed that DO might affect diverse signaling pathways related to the pathogenesis of hyperlipidemia, including PPAR signaling pathway, insulin resistance, AMPK signaling pathway, and non-alcoholic fatty liver disease simultaneously. Meanwhile, in the HFDA-induced hyperlipidemia rat model, DO could significantly decrease the level of TC, TG, LDL-c, and ALT in serum, and increase HDL-c as well. The liver pathological section indicated that DO could ease liver damage and lipid cumulation.In summary, the biological targets of the main bioactive compounds in DO were found to distribute across multiple metabolic pathways. These findings suggest that a mutual regulatory system consisting of multiple components, targets, and pathways is a likely mechanism through which DO may improve hyperlipidemia. Validation experiments indicated that DO may treat hyperlipidemia by affecting NAFLD-related signaling pathways.
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- 2021
19. Soporific effect of modified Suanzaoren Decoction on mice models of insomnia by regulating Orexin-A and HPA axis homeostasis
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Ning-Hua Jiang, Su-Hong Chen, Min-Qiu Lin, Xi Teng, Xi Fang, Lin-Zi Li, Zhi-Yi Xie, Rong Luo, Gui-Yuan Lv, Bo Li, Bei-Bei Zhang, Ying-Jie Dong, and Liang-Hui Zhan
- Subjects
Male ,medicine.medical_specialty ,Hypothalamo-Hypophyseal System ,Insomnia ,Orexin-A ,5-HT ,Stimulation ,RM1-950 ,Adrenocorticotropic hormone ,Modified Suanzaoren Decoction (MSZRD) ,Orexin Receptors ,Internal medicine ,Sleep Initiation and Maintenance Disorders ,Adrenal Glands ,medicine ,Animals ,Circadian rhythm ,5-HT receptor ,Pharmacology ,Mice, Inbred ICR ,Neurotransmitter Agents ,Orexins ,Behavior, Animal ,business.industry ,OX2R ,HPA axis ,General Medicine ,Orexin receptor ,Disease Models, Animal ,Endocrinology ,Hypothalamus ,Sleep Aids, Pharmaceutical ,Therapeutics. Pharmacology ,business ,Sleep ,Hormone ,Drugs, Chinese Herbal ,Signal Transduction - Abstract
Aim: Modified Suanzaoren Decoction (MSZRD) is obtained by improving Suanzaoren Decoction (SZRT), a traditional Chinese herbal prescription that has been used to treat insomnia for more than thousands of years. Our previous study showed that MSZRD can improve the gastrointestinal discomfort related insomnia by regulating Orexin-A. This study is the first study to evaluate the effects and possible mechanisms of MSZRD in mice with insomnia caused by p-chlorophenylalanine (PCPA) combined with multifactor random stimulation. Methods: After 14 days of multifactor stimulation to ICR mice, a PCPA suspension (30 mg/mL) was injected intraperitoneally for two consecutive days to establish an insomnia model. Three different doses of MSZRD (3.6, 7.2, and 14.4 g/kg/day) were given to ICR mice for 24 days. The food intake and back temperature were measured, and behavioral tests and pentobarbital sodium-induced sleep tests were conducted. The levels of Orexin-A, corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), and adrenocortical hormones (CORT) in the serum and 5-hydroxytryptamine (5-HT), dopamine (DA), and norepinephrine (NE) in hypothalamus were measured using enzyme-linked immunosorbent assay (ELISA) kits. The levels of γ-aminobutyric acid (GABA) and glutamic acid (Glu) were measured by high-performance liquid chromatography (HPLC). The expression of 5HT1A receptor (5-HTRIA) and orexin receptor 2 antibody (OX2R) was measured by Western blot (WB) and immunohistochemical staining (ICH). Hematoxylin and eosin (H&E) staining and Nissl staining were used to assess the histological changes in hypothalamus tissue. Results: Of note, MSZRD can shorten the sleep latency of insomnia mice (P
- Published
- 2021
20. Effect of anti-PMSG on distribution of estrogen receptor alpha and progesterone receptor in mouse ovary, oviduct and uterus
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Lin, Zi Li, Ni, He Min, Liu, Yun Hai, Sheng, Xi Hui, Cui, Xiang Shun, Kim, Nam Hyung, and Guo, Yong
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- 2015
- Full Text
- View/download PDF
21. Based on the expression of HSP60 and HSP90, to explore the effect of Xuduan Zhongzi prescription on semen quality of oligoasthenospermia model rats.
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ZHANG Ya-guang, WANG Quan-sheng, HUANG Zi-yan, Chen Lu, SONG Ji-xiang, and LIN Zi-li
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SEMEN analysis ,SERTOLI cells ,EXFOLIATION syndrome ,HEAT shock proteins ,CELL death ,MALE infertility ,SEMINIFEROUS tubules ,LEVOTHYROXINE - Abstract
Objective: To investigate the effect of Xuduan Zhongzi prescription on the expression of HSP60 and HSP90 in testicular spermatogenic cells and semen quality of oligozoospermia and asthenospermia model rats. Methods: A total of 40 SPF male rats were randomly divided into 10 blank control group and 30 model group. After successful modeling, the model group was divided into model control group, levocarnitine group and Xuduan Zhongzi prescription group with 10 rats in each group. The rats in levocarnitine group and Xuduan Zhongzi prescription group were given corresponding drugs by gavage according to the equivalent measurement of humanbody, the blank control group and model control group were given the same amount of normal saline by gavage, and the rats in each group were killed after 8 weeks of continuous administration. Take the left epididymis of each group for sperm quality detection, take the left testis of each group, observe the histomorphological changes by HE staining, and detect the expression of HSP60 and HSP90 in testicular tissue by immunohistochemistry and Western blot. Results: HE staining showed that compared with the blank control group, the convoluted tubules in the model group were significantly atrophic, degenerated and irregularly arranged, the spermatogenic epithelium showed a large number of vacuoles, Sertoli cell degeneration, obvious inflammatory infiltration in stromal cells, stromal cell proliferation, scattered rows of epithelial cells, a large number of exfoliation and degeneration, and sperm were rare. After the intervention of Xuduan Zhongzi prescription, the above lesions were significantly improved, the structure of seminiferous tubules was relatively regular and orderly, denser than that of the model group, sertoli cells and stromal cells increased significantly, the seminiferous epithelium was thicker than that of the model group, with rich layers, and a large number of sperm could be seen in the lumen. Immunohistochemistry and Western Blot showed that the expression of HSP60 and HSP90 increased significantly after the intervention of Xuduan Zhongzi prescription in model rats (P<0.01). The sperm quality test results showed that compared with the blank control group, the sperm concentration and activity rate in the model group decreased significantly, (P<0.01). After continuous seed cutting, the sperm concentration and activity rate of model rats were significantly increased, (P<0.01). Conclusion: Xuduan Zhongzi prescription may inhibit the apoptosis of testicular spermatogenic cells in oligoasthenospermia model rats by increasing the expression of HSP60 and HSP90, so as to improve sperm quality. [ABSTRACT FROM AUTHOR]
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- 2022
22. Dendrobium officinale Regulates Fatty Acid Metabolism to Ameliorate Liver Lipid Accumulation in NAFLD Mice
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Bo Li, Fu-Chen Zhou, Hai-Ying Jin, Su-Hong Chen, Qiao-Xian Yu, Ying-Jie Dong, Hui-Ying Wang, Gui-Yuan Lv, Rong Luo, Xiang Zheng, Lin-Zi Li, Ning-Yu Zhang, Shan-Shan Lei, and Xinglishang He
- Subjects
medicine.medical_specialty ,Article Subject ,03 medical and health sciences ,chemistry.chemical_compound ,Other systems of medicine ,0302 clinical medicine ,Internal medicine ,Nonalcoholic fatty liver disease ,medicine ,Carnitine ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,biology ,Fatty acid metabolism ,Triglyceride ,Fatty acid ,Metabolism ,Shotgun lipidomics ,medicine.disease ,Fatty acid synthase ,Endocrinology ,Complementary and alternative medicine ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,lipids (amino acids, peptides, and proteins) ,RZ201-999 ,Research Article ,medicine.drug - Abstract
Dendrobium officinale (DOF) is a traditional Chinese edible and officinal plant. Ultrafine DOF powder (DOFP) can regulate lipids and histopathology in the liver, but the underlying mechanisms of hepatic fatty acid (FA) metabolism, which is generally correlated with the development of nonalcoholic fatty liver disease (NAFLD), remain unclear. The purpose of the present study was to investigate whether DOFP treatment alters hepatic FA metabolism in NAFLD mice by using multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) and analyse the underlying mechanisms. A 3-week DOFP treatment prevented lipid deposition and improved hepatic histopathology in NAFLD mice after withdrawal from the high-sucrose, high-fat (HSHF) diet, and it decreased triglyceride and FA content in the liver. Furthermore, the C16 : 0/C14 : 0 and C18 : 1/18 : 0 ratios in FAs were significantly decreased in the DOFP treatment group, and the C20 : 4/C20 : 3 and C22 : 4/C22 : 3 ratios were increased, and saturated FA was inhibited. Additionally, DOFP treatment significantly increased the content of two FA β-oxidation-related proteins (carnitine palmitoyltransferase 1-α and acyl-coenzyme A oxidase 1). It also decreased the content of a FA synthesis-related protein (fatty acid synthase), a FA desaturation-related protein (stearoyl-coenzyme A desaturase-1), and a FA uptake-related protein (fatty acid transport protein 2). Moreover, DOFP treatment improved dysregulated levels of major phospholipids in the livers of model mice. The results of this study confirm that DOFP treatment in NAFLD mice has liver recovery effects by regulating FA metabolism.
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- 2021
23. Benefit Effect of Dendrobium officinale Ultrafine Powder on DSS-Induced Ulcerative Colitis Rats by Improving Colon Mucosal Barrier
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Xiang Zheng, Tao-Xiu Xiong, Ke Zhang, Fu-Chen Zhou, Hui-Ying Wang, Bo Li, Ying-Jie Dong, Xinglishang He, Lin-Zi Li, Qiao-Xian Yu, Gui-Yuan Lv, and Su-Hong Chen
- Subjects
Other systems of medicine ,Article Subject ,Complementary and alternative medicine ,RZ201-999 ,Research Article - Abstract
Aim and Objective. To study the effect of Dendrobium officinale ultrafine powder (DOFP) on the intestinal mucosal barrier in rats with ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). Materials and Methods. After intragastric administration of DOFP for 3 weeks, the rat UC model was made by the administration of 4% oral DSS solution for one week, and the drug was given at the same time. During the experiment, the disease activity index (DAI) score of the rats was regularly computed. At the end of the experiment, the blood routine indexes of rats were obtained. The histopathological changes in the colon were monitored by hematoxylin-eosin (H&E) and PAS staining and observation of ultrastructural changes in the colon by transmission electron microscope. Occludin expression in the colon was monitored by Western blot, the expression of claudin-1 and ZO-1 in the colon was detected by immunofluorescence, and the expression of TNF-α, IL-6, and IL-1β in the colon was detected by immunohistochemistry. Results. The results firstly indicated that DOFP could significantly alleviate the signs and symptoms of the DSS-induced rats UC model, which manifested as improvement of body weight loss, increase of colon length, and improvement of the symptoms of diarrhea and hematochezia. Then, results from histopathology, blood routine examination, and transmission electron microscope analysis further implied that DOFP could dramatically reduce inflammatory cell infiltration and restore intestinal epithelial barrier integrity. In addition, the experiments of Western Blot analysis, immunofluorescence, and PAS staining also further confirmed that DOFP could markedly increase related protein expressions of the intestinal barrier and mucus barrier, as the expression of occludin, claudin-1, and ZO-1 in the colon significantly decreased. The experiments of immunohistochemistry confirmed that DOFP could markedly decrease protein expression levels of inflammatory cytokines TNF-α, IL-6, and IL-1β. Conclusion. DOFP notably alleviated inflammatory lesions, repaired the colon mucosa damage by promoting the expression of tight junction proteins occludin, claudin-1, and ZO-1 and inhibiting the release of inflammatory factors TNF-α, IL-6, and IL-1β, and finally achieved the purpose of treating UC.
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- 2021
24. Effects of Macroporous Resin Extract of Dendrobium officinale Leaves in Rats with Hyperuricemia Induced by Fructose and Potassium Oxonate
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Xinglishang He, Yu-Zhi Wang, Bo Li, Qiao-Xian Yu, Cong Zhou, Wan-Feng Xu, Su-Hong Chen, Xiang Zheng, Ying-Jie Dong, Lin-Zi Li, Li-Jie Zhu, and Gui-Yuan Lv
- Subjects
Xanthine Oxidase ,Potassium ,chemistry.chemical_element ,Fructose ,Hyperuricemia ,Pharmacology ,Kidney ,Excretion ,chemistry.chemical_compound ,Adenosine Triphosphate ,Drug Discovery ,medicine ,Animals ,Xanthine oxidase ,Plant Extracts ,Organic Chemistry ,Glucose transporter ,General Medicine ,medicine.disease ,Computer Science Applications ,Rats ,Uric Acid ,Plant Leaves ,Oxonic Acid ,medicine.anatomical_structure ,chemistry ,Uric acid ,Dendrobium - Abstract
Aim and Objective: Fructose, as a ubiquitous monosaccharide, can promote ATP consumption and elevate circulating Uric acid (UA) levels. Our previous studies have confirmed that the macroporous resin extract of Dendrobium officinale leaves (DoMRE) could reduce the UA level of rats with hyperuricemia induced by a high-purine diet. This study aimed to investigate whether DoMRE had a UA-lowering effect on rats with hyperuricemia caused by fructose combined with potassium oxonate, so as to further clarify the UA-lowering effect of DoMRE, and to explore the UAlowering effect of DoMRE on both UA production and excretion. Materials and Methods: Rats with hyperuricemia induced by fructose and potassium oxonate were administered with DoMRE and vehicle control, respectively, to compare the effects of the drugs. At the end of the experiment, the Serum Uric Acid (SUA) and Creatinine (Cr) levels were measured using an automatic biochemical analyzer, the activities of xanthine oxidase (XOD) were measured using an assay kit, and the protein expressions of Urate Transporter 1 (URAT1), glucose transporter 9 (GLUT9), and ATP-Binding Cassette Superfamily G member 2 (ABCG2) were assessed using immune-histochemical and western blot analyses. Hematoxylin and eosin staining was used to assess the histological changes in the kidney, liver, and intestine. Results: Fructose and potassium induced hyperuricemia in rats. Meanwhile, the activities of XOD were markedly augmented, the expression of URAT1 and GLUT9 was promoted, and the expression of ABCG2 was reduced, which were conducive to the elevation of UA. However, exposure to DoMRE reversed these fructose- and potassium oxonate-induced negative alternations in rats. The activities of XOD were recovered to the normal level, reducing UA formation; the expressions of URAT1, ABCG2, and GLUT9 returned to the normal level, resulting in an increase in renal urate excretion. Conclusion: DoMRE reduces UA levels in rats with hyperuricemia induced by fructose combined with potassium oxonate by inhibiting XOD activity and regulating the expression of ABCG2, URAT1, and GLUT9. DoMRE is a potential therapeutic agent for treating hyperuricemia through inhibiting UA formation and promoting UA excretion.
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- 2020
25. Hypertensive Rats Treated Chronically With Nω-Nitro-L-Arginine Methyl Ester (L-NAME) Induced Disorder of Hepatic Fatty Acid Metabolism and Intestinal Pathophysiology
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Ye-Hui Chen, Jing-Jing Yu, Jie Su, Yu-Zhi Wang, Lin-Zi Li, Shan-Shan Lei, Gui-Yuan Lv, Su-Hong Chen, Rong Luo, Bo Li, Xinglishang He, Dorota Kołodyńska, Fu-Chen Zhou, Shan Xiong, Ning-Yu Zhang, and Xiang Zheng
- Subjects
0301 basic medicine ,medicine.medical_specialty ,hypertension ,Aspartate transaminase ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) ,intestinal pathophysiology ,Internal medicine ,medicine ,Pharmacology (medical) ,chemistry.chemical_classification ,Liver injury ,Pharmacology ,biology ,Fatty acid metabolism ,Triglyceride ,lcsh:RM1-950 ,Fatty acid ,Shotgun lipidomics ,medicine.disease ,030104 developmental biology ,Endocrinology ,lcsh:Therapeutics. Pharmacology ,Alanine transaminase ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,fatty acid ,liver injury - Abstract
Nω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) biosynthesis, results in hypertension and liver injury. This study aimed at investigating the changes of liver lipometabonomics and exploring the underlying mechanisms of liver injury in the L-NAME-treated rats. The male Sprague-Dawley (SD) rats were treated with L-NAME (40 mg/kg, p.o.) for 8 weeks. After that, the liver, aorta, fecal, and serum were collected for analysis. The results showed that L-NAME induced hypertension and disordered the endothelial nitric oxide synthase (eNOS)-NO pathway in the treated rats. L-NAME could also increase the levels of serum total cholesterol (TC), triglyceride (TG), alanine transaminase (ALT), and aspartate transaminase (AST). The multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) analysis showed that L-NAME could induce significant changes of the total hepatic lipids and most hepatic triglycerides, as well as fatty acid (FA). A positive correlation was found between the blood pressure and TAG. Immunofluorescence and Western-Blot experiments indicated that the L-NAME treatment significantly influenced some FA β-oxidation, desaturation, and synthesis-related proteins. The increase of intestinal inflammation, decrease of microcirculation and tight junction proteins, as well as alterations of microbial communities were observed in the L-NAME induced hypertensive rats, as well as alterations of microbial communities were notable correlation to TAG and FA species. This study demonstrated that the L-NAME-induced hypertensive rats exhibiting liver injury were the joint action of hepatic abnormal fatty acid metabolism and microcirculation disorder. Furthermore, the gut microflora, as well as the changes of FA β-oxidation (ACOX, CPT1α), desaturation (SCD-1), and synthesis (FAS) may be the potential mechanisms for abnormal fatty acid metabolism.
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- 2020
26. Hypertensive Rats Treated Chronically With N
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Bo, Li, Xinglishang, He, Shan-Shan, Lei, Fu-Chen, Zhou, Ning-Yu, Zhang, Ye-Hui, Chen, Yu-Zhi, Wang, Jie, Su, Jing-Jing, Yu, Lin-Zi, Li, Xiang, Zheng, Rong, Luo, Dorota, Kołodyńska, Shan, Xiong, Gui-Yuan, Lv, and Su-Hong, Chen
- Subjects
Pharmacology ,hypertension ,multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) ,intestinal pathophysiology ,fatty acid ,Original Research ,liver injury - Abstract
Nω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) biosynthesis, results in hypertension and liver injury. This study aimed at investigating the changes of liver lipometabonomics and exploring the underlying mechanisms of liver injury in the L-NAME-treated rats. The male Sprague-Dawley (SD) rats were treated with L-NAME (40 mg/kg, p.o.) for 8 weeks. After that, the liver, aorta, fecal, and serum were collected for analysis. The results showed that L-NAME induced hypertension and disordered the endothelial nitric oxide synthase (eNOS)-NO pathway in the treated rats. L-NAME could also increase the levels of serum total cholesterol (TC), triglyceride (TG), alanine transaminase (ALT), and aspartate transaminase (AST). The multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) analysis showed that L-NAME could induce significant changes of the total hepatic lipids and most hepatic triglycerides, as well as fatty acid (FA). A positive correlation was found between the blood pressure and TAG. Immunofluorescence and Western-Blot experiments indicated that the L-NAME treatment significantly influenced some FA β-oxidation, desaturation, and synthesis-related proteins. The increase of intestinal inflammation, decrease of microcirculation and tight junction proteins, as well as alterations of microbial communities were observed in the L-NAME induced hypertensive rats, as well as alterations of microbial communities were notable correlation to TAG and FA species. This study demonstrated that the L-NAME-induced hypertensive rats exhibiting liver injury were the joint action of hepatic abnormal fatty acid metabolism and microcirculation disorder. Furthermore, the gut microflora, as well as the changes of FA β-oxidation (ACOX, CPT1α), desaturation (SCD-1), and synthesis (FAS) may be the potential mechanisms for abnormal fatty acid metabolism.
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- 2019
27. Associations of sleep duration and sleep quality with life satisfaction in elderly Chinese: The mediating role of depression
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Min-Jie Xu, Xuefeng Chu, Shu-Juan Li, Ting-Fan Zhi, Qun-Shan Wang, Xun-Ming Sun, Yan-xun Li, Yong Wang, Jian Cai, Lin-zi Li, Zhengdong Wang, and Xiaoyan Jiang
- Subjects
Male ,China ,Aging ,Mediation (statistics) ,medicine.medical_specialty ,Time Factors ,Health (social science) ,Cross-sectional study ,Personal Satisfaction ,Logistic regression ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,medicine ,Humans ,030212 general & internal medicine ,Psychiatry ,Depression (differential diagnoses) ,Aged ,Aged, 80 and over ,Sleep quality ,Depression ,Life satisfaction ,Sleep in non-human animals ,Cross-Sectional Studies ,Female ,Geriatrics and Gerontology ,Sleep ,Psychology ,Gerontology ,030217 neurology & neurosurgery ,Demography ,Sleep duration - Abstract
This study investigated whether sleep duration and quality were related to life satisfaction (LS) among older Chinese adults and whether depression mediated those relationships. Cross-sectional data from the aging arm of the Rugao Longevity and Aging Study were used. Sleep duration, sleep quality, depression, LS and covariates were analyzed using logistic regressions. To assess the potential mediation of depression on the association between sleep duration and quality and LS, Aroian tests were used. Of 1756 older Chinese adults aged 70-84 years, 90.7% of the men and 83.3% of the women reported being satisfied with their lives. After adjusting for covariates, older adults who slept ≤6h per night were more likely to suffer from life dissatisfaction compared with those who slept 7-8h (OR=2.67, 95% CI 1.86-3.79), and individuals who slept poorly were almost 2 times (OR=2.91, 95% CI 2.16-3.91) more likely to have life dissatisfaction. The Aroian tests confirmed that these relationships were partially mediated by depression (p
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- 2016
28. A Maternal Transcription Factor, Junction Mediating and Regulatory Protein is Required for Preimplantation Development in the Mouse
- Author
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Lin, Zi-Li, primary, Li, Ying-Hua, additional, Jin, Yong-Xun, additional, and Kim, Nam-Hyung, additional
- Published
- 2019
- Full Text
- View/download PDF
29. The Impact Analysis of Revolute Pair Clearance to the End Pose Accuracy of Welding Robot
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Yin Lin Pu, Hui Lin, Lin Zi Li, Zhu Xin, and Fa Jun Zhang
- Subjects
Engineering ,business.industry ,Hinge ,General Medicine ,Structural engineering ,Welding ,Revolute joint ,Displacement (vector) ,law.invention ,Robot welding ,Control theory ,Position (vector) ,law ,business ,Joint (geology) ,Robotic arm - Abstract
Various uncertain factors affect the movement of the welding robot, thus welding gun tend to deviate from the theory of welding position which reduces the welding accuracy, of which the revolute pair clearance have an greater effect on the movement of the welding robot. In order to study the influence of revolute pair clearance to the end pose accuracy of welding robot, the mathematical model of revolute pair clearance was established, and the software SolidWorks was used for establishing the welding robot model, making simulations of the mechanical arm with joint clearance and no joint clearance. At last, the movement characteristic of the hinge shaft is attained. The simulation results showed that the shaft velocity and displacement of mechanical arm with joint clearance has a certain degree of fluctuation, which affecting the end pose accuracy of welding robot , and reducing the movement stability and the welding accuracy of welding robot.
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- 2015
30. Cleaner Production Potential of Industries in Liao River Basin
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Peng Shen, Ze Qiang Fu, and Lin Zi Li
- Subjects
Pollutant ,geography ,geography.geographical_feature_category ,General Engineering ,Environmental engineering ,Drainage basin ,Production (economics) ,Environmental science ,Cleaner production ,Structural basin - Abstract
Through the key industries identification process, this study identified seven of key industries in Liao River basin, to evaluate the cleaner production potential of industries in the basin. The results indicated that, without the adverse impact on the economic growth, by 2020, under the high cost cleaner production solution, COD and NH3-N production would be 17.02% and 19.17% less than that of 2012, so that industrial emissions of major pollutants in the basin could be 15% less in comparison with that in 2012, as long as the end-of-pipe treatment ratio maintains the level in 2012.
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- 2014
31. Retinoic acid protects from experimental cerebral infarction by upregulating GAP-43 expression
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Ying-fei Li, Hailiang Liu, Yalong Yang, Qiaoxuan Wang, Xiaoya Gao, Lin Zi Li, and Biliang Zhang
- Subjects
Male ,0301 basic medicine ,Time Factors ,Physiology ,medicine.medical_treatment ,Retinoic acid ,Gene Expression ,Infarction ,Biochemistry ,Brain Ischemia ,Rats, Sprague-Dawley ,Random Allocation ,chemistry.chemical_compound ,GAP-43 Protein ,0302 clinical medicine ,General Pharmacology, Toxicology and Pharmaceutics ,lcsh:QH301-705.5 ,lcsh:R5-920 ,Cerebral infarction ,General Neuroscience ,Infarction, Middle Cerebral Artery ,General Medicine ,Immunohistochemistry ,Up-Regulation ,Neuroprotective Agents ,lcsh:Medicine (General) ,medicine.medical_specialty ,Immunology ,Immunocytochemistry ,Intraperitoneal injection ,Biophysics ,Ischemia ,Tretinoin ,Ocean Engineering ,Growth associate protein 43 ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Downregulation and upregulation ,Internal medicine ,medicine ,Animals ,cardiovascular diseases ,Messenger RNA ,business.industry ,Reproducibility of Results ,Biomedical Sciences ,Cell Biology ,medicine.disease ,Focal cerebral infarction ,Nerve regeneration ,030104 developmental biology ,Endocrinology ,lcsh:Biology (General) ,chemistry ,business ,030217 neurology & neurosurgery - Abstract
The aim of this study was to investigate whether exogenous retinoic acid (RA) can upregulate the mRNA and protein expression of growth-associated protein 43 (GAP-43), thereby promoting brain functional recovery in a rat distal middle cerebral artery occlusion (MCAO) model of ischemia. A total of 216 male Sprague Dawley rats weighing 300–320 g were divided into 3 groups: sham-operated group, MCAO+vehicle group and MCAO+RA group. Focal cortical infarction was induced with a distal MCAO model. The expression of GAP-43 mRNA and protein in the ipsilateral perifocal region was assessed using qPCR and immunocytochemistry at 1, 3, 7, 14, 21, and 28 days after distal MCAO. In addition, an intraperitoneal injection of RA was given 12 h before MCAO and continued every day until the animal was sacrificed. Following ischemia, the expression of GAP-43 first increased considerably and then decreased. Administration of RA reduced infarction volume, promoted neurological functional recovery and upregulated expression of GAP-43. Administration of RA can ameliorate neuronal damage and promote nerve regeneration by upregulating the expression of GAP-43 in the perifocal region after distal MCAO.
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- 2017
32. Study on Dynamic Response of Joints Accuracy to the Slider Crank Mechanism of Internal Combustion Engine
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Yin Lin Pu, Hui Lin, Fa Jun Zhang, Xin Zhu, Shan Shan Wang, and Lin Zi Li
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Engineering ,Crank ,business.industry ,General Medicine ,Structural engineering ,Stability (probability) ,Mechanism (engineering) ,Nonlinear system ,Internal combustion engine ,Spring (device) ,Control theory ,Slider ,business ,Reliability (statistics) - Abstract
Nonlinear equivalent spring resistance model and Coulomb friction model is used to construct a dynamic mathematical model for a multi-body system, which contains clearance. In the paper an internal combustion engine dynamic model has created for simulated analysis. It shows that joints accuracy and driver speed have obvious effects on crank slider dynamic disturbance. Also joints clearance and the increase of driver speed lead to lower positional accuracy, worse stability and reliability, which has very important theoretical significance and practical value on the analysis of the performance of internal combustion engine.
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- 2014
33. FoxD3-regulated microRNA-137 suppresses tumour growth and metastasis in human hepatocellular carcinoma by targeting AKT2
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Shi Xun Lu, Wen Hu, Min Li, Chris Zhiyi Zhang, Li Li Liu, Jia Fu, Rong Zhen Luo, Jing Ping Yun, Yuan Zhong Yang, and Lin Zi Li
- Subjects
Male ,Carcinoma, Hepatocellular ,Blotting, Western ,Mice, Nude ,FoxD3 ,AKT2 ,miR-137 ,Metastasis ,Cell Movement ,microRNA ,medicine ,metastasis ,Animals ,Humans ,Gene silencing ,HCC ,Neoplasm Metastasis ,3' Untranslated Regions ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Mice, Inbred BALB C ,Base Sequence ,Reverse Transcriptase Polymerase Chain Reaction ,business.industry ,Cell growth ,Liver Neoplasms ,Forkhead Transcription Factors ,Middle Aged ,Prognosis ,medicine.disease ,Xenograft Model Antitumor Assays ,Tumor Burden ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,mir-137 ,Oncology ,Hepatocellular carcinoma ,embryonic structures ,Cancer research ,Female ,RNA Interference ,business ,Proto-Oncogene Proteins c-akt ,hormones, hormone substitutes, and hormone antagonists ,Research Paper - Abstract
// Li-Li Liu 1,2,* , Shi-Xun Lu 1,2,* , Min Li 1,2 , Lin-Zi Li 1,2 , Jia Fu 1,2 , Wen Hu 1,2 , Yuan-Zhong Yang 1,2 , Rong-Zhen Luo 1,2 , Chris Zhiyi Zhang 1,2 , Jing-Ping Yun 1,2 1 Sun Yat-sen University Cancer Center; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine 2 Department of Pathology, Sun Yat-sen University Cancer Center * These authors contributed equally to this work Correspondence: Chris Zhiyi Zhang, email: // Jing-Ping Yun, email: // Keywords : miR-137; AKT2; FoxD3; metastasis; HCC Received : May 3, 2014 Accepted : June 9, 2014 Published : June 10, 2014 Abstract microRNAs, frequently deregulated in human cancer, have been implicated in the progression of hepatocarcinogenesis. Here, we show that microRNA (miR)-137 is significantly down-regulated in hepatocellular carcinoma (HCC). Its decreased expression is associated with vein invasion, incomplete involucrum, and distant metastasis. Multivariate analysis suggests that miR-137 is an independent indicator for poor survival. We next show that over-expression of miR-137 suppresses cell proliferation, migration and invasion in vitro . Conversely, miR-137 inhibition promotes HCC cell growth. We also identify AKT2 as a key target of miR-137 in this context. Statistical data reveal a reverse correlation of AKT2 and miR-137 expression in HCC patients. Silencing of AKT2 phenotypically copied miR-137-induced phenotypes, whereas re-expression of AKT2 reversed the suppressive effects of miR-137. Further investigations showed that miR-137 exerted its anti-tumour activity via inhibiting the AKT2/mTOR pathway. Moreover, we demonstrate that FoxD3 directly binds to the promoter of miR-137 and activates its transcription. In vivo studies confirm that FoxD3-regulated miR-137 inhibited HCC growth and metastasis via targeting AKT2. Together, our findings indicate that miR-137 is a valuable biomarker for HCC prognosis and the FoxD3/miR-137/AKT2 regulatory network plays an important role in HCC progression.
- Published
- 2014
34. The Empirical Study on Industrial Park Circular Economy Transformation - Binxi Industrial Park
- Author
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Jing Guo, Ze Qiang Fu, Lin Zi Li, and Peng Shen
- Subjects
Engineering ,Resource (biology) ,Process (engineering) ,business.industry ,Circular economy ,General Engineering ,Environmental economics ,Civil engineering ,Empirical research ,Agriculture ,Industrial park ,Production (economics) ,Industrial ecology ,business - Abstract
Circular economy transformation of industrial park refers to reforming and upgrading the traditional industrial park in accordance with the concept of circular economy, with the focus on the transformation and construction of the industrial structure, infrastructure, production technology, management mechanism and so on, thus improving the utilization efficiency of resource and energy, and reducing pollution emissions. Taking Binxi industrial Park as an example, based on the problems existing in the development process, this paper determines the main tasks of circular economy transformation and constructs the main recycling industry chains, providing a model for the circular economy transformation of the industrial parks that are agriculture and Forestry based.
- Published
- 2014
35. Analysis and Optimization of Industrial Structure Based on the Relative Efficiency Index
- Author
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Bao Gao, Yuan Yuan Xie, Peng Shen, Ze Qiang Fu, Na Wu, and Lin Zi Li
- Subjects
Engineering ,Index (economics) ,Efficiency ,Textile ,Waste management ,business.industry ,Manufacturing ,General Engineering ,Production (economics) ,Electricity ,Eco-efficiency ,Raw material ,business - Abstract
Based on the theory and method of eco-efficiency, the relative efficiency index (Ri) was constructed in the paper. According to the range of the relative efficiency index, the industrial sectors can be divided into four categories. When Ri≥R+0.5δ , the industry should be encouraged. When R≤Ri
- Published
- 2014
36. Can Biological Fuels Replace Fossil Fuels? - A Critical Thinking
- Author
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Ze Qiang Fu and Lin Zi Li
- Subjects
Competition (economics) ,Engineering ,Biodiversity conservation ,Critical thinking ,Natural resource economics ,business.industry ,Biofuel ,Fossil fuel ,General Engineering ,Environmental engineering ,business ,Social equality - Abstract
Biological fuels have triggered the heated controversy in recent years. This paper critically examined the potentials of biological fuels through the lens of Multi-Criteria Analysis (MCA) and Integrated Conservation and Development Projects (ICDP). Although MCA framework may possess the capability in making the right decision on land diversion to handle the intensifying competition for land between biofuels and food production, it is difficult to reach a balance among sufficient biofuels supply, biodiversity conservation and social equity from the perspective of ICDP. Despite biofuels could not totally displace fossil fuels in the near future, there might still be some possible policy designs that can help realizing the smooth transition to the biofuels era.
- Published
- 2014
37. Knockdown of nucleophosmin induces S-phase arrest in HepG2 cells
- Author
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Jing Ping Yun, Jian Yong Xiao, Zhi Yi Zhang, Chun Yi, Lin Zi Li, Qing Qing Wang, and Yan Huang
- Subjects
p53 ,Cyclin-Dependent Kinase Inhibitor p21 ,Biology ,S Phase ,Ribosome assembly ,Flow cytometry ,medicine ,Humans ,Centrosome duplication ,RNA, Small Interfering ,Cell Proliferation ,Nucleophosmin ,Gene knockdown ,Antibiotics, Antineoplastic ,integumentary system ,medicine.diagnostic_test ,Cell growth ,Cell Cycle ,p21Cip1/WAF1 ,Nuclear Proteins ,Hep G2 Cells ,Cell cycle ,Molecular biology ,Oncology ,Apoptosis ,Gene Knockdown Techniques ,Dactinomycin ,actinomycin D ,Original Article ,Tumor Suppressor Protein p53 - Abstract
Nucleophosmin/B23 (NPM) is a universally expressed nucleolar phosphoprotein that participates in proliferation, apoptosis, ribosome assembly, and centrosome duplication; however, the role of NPM in cell cycle regulation is not well characterized. We investigated the mechanism by which NPM is involved in cell cycle regulation. NPM was knocked down using siRNA in HepG2 hepatoblastoma cells. NPM translocation following actinomycin D (ActD) treatment was investigated using immunofluorescent staining. Expression of NPM and other factors involved in cell cycle regulation was examined by Western blotting. Cell cycle distribution was measured using flow cytometry to detect 5-ethynyl-2'-deoxyuridine (EdU) incorporation. Cell proliferation was quantified by the MTT assay. Knockdown of NPM increased the percentage of HepG2 cells in S phase and led to decreased expression of P53 and P21Cip1/WAF1. S-phase arrest in HepG2 cells was significantly enhanced by ActD treatment. Furthermore, knockdown of NPM abrogated ActD-induced G2/M phase cell cycle arrest. Taken together, these data demonstrate that inhibition of NPM has a significant effect on the cell cycle.
- Published
- 2011
38. Association of CRP gene polymorphisms with CRP levels, frailty and co-morbidity in an elderly Chinese population: results from RuLAS
- Author
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Zuyun Liu, Yong Wang, Lin-zi Li, Jianming Shi, Zhengdong Wang, Xiaofeng Wang, Li Jin, Yinsheng Zhu, Xuejuan Xie, and Xuefeng Chu
- Subjects
Male ,medicine.medical_specialty ,Aging ,China ,Population ,Longevity ,Single-nucleotide polymorphism ,Comorbidity ,Polymorphism, Single Nucleotide ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Internal medicine ,Genotype ,Confidence Intervals ,Odds Ratio ,Medicine ,Humans ,030212 general & internal medicine ,education ,Geriatric Assessment ,Aged ,Aged, 80 and over ,education.field_of_study ,business.industry ,General Medicine ,Odds ratio ,Survival Analysis ,Confidence interval ,Minor allele frequency ,C-Reactive Protein ,Population study ,Female ,Disease Susceptibility ,Geriatrics and Gerontology ,business ,030217 neurology & neurosurgery ,Cohort study - Abstract
OBJECTIVES to examine the associations of two common CRP gene polymorphisms with CRP levels, frailty and co-morbidity in an elderly Chinese population. DESIGN a population-based cohort study. SETTING AND PARTICIPANTS we obtained data on 1,723 elderly participants aged 70-84 from the ageing arm of the Rugao Longevity and Ageing study (RuLAS), a population-based observational cohort study conducted in Rugao, Jiangsu province, China. MEASUREMENTS the genotyping of two common CRP gene polymorphisms (rs1205 and rs3093059) was performed. Items concerning the frailty index and co-morbidity were collected. RESULTS the mean age of the study population was 75.3 ± 3.9 years, and 53.5% (n = 922) were women. The minor allele frequencies of rs1205 and rs3093059 were 42.4% (C allele) and 16.9% (C allele), respectively. The polymorphisms rs1205 and rs3093059 were significantly associated with CRP levels (β = 0.113 and 0.222, all P < 0.001). Non-significant association between rs1205 and rs3093059 and frailty, as well as between rs3093059 and co-morbidity was observed. However, SNP rs1205 CC genotype had an increased odds of co-morbidity compared with the TT genotype (odds ratio (OR):1.53; 95% confidence interval (CI): 1.16-2.02). Each additional copy of the C allele of SNP rs1205 was associated with 1.23 times (95% CI: 1.07-1.41) odds of co-morbidity. The significance remained after controlling for covariates such as education level, etc. CONCLUSIONS among elderly Chinese individuals, two CRP gene polymorphisms were significantly associated with CRP levels. However, none of them was associated with frailty. The preliminary findings warrant further validations.
- Published
- 2015
39. Low local blood perfusion, high white blood cell and high platelet count are associated with primary tumor growth and lung metastasis in a 4T1 mouse breast cancer metastasis model
- Author
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Xin Ji, Ji-Zhong Yan, Qiao‑Li Qiu, Su‑Hong Chen, Jie Su, Gui‑Yuan Lyu, Han‑Ting Xu, Jian-Li Gao, Qi Zhang, Yue‑Li Zhang, Ying Ge Chen, Lin‑Zi Li, and Chuan Wang
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Cancer ,Mean corpuscular hemoglobin ,Blood flow ,Articles ,medicine.disease ,Primary tumor ,Metastasis ,medicine.anatomical_structure ,Breast cancer ,Oncology ,White blood cell ,medicine ,Blood test ,business - Abstract
It was originally thought that no single routine blood test result would be able to indicate whether or not a patient had cancer; however, several novel studies have indicated that the median survival and prognosis of cancer patients were markedly associated with the systemic circulation features of cancer patients. In addition, certain parameters, such as white blood cell (WBC) count, were largely altered in malignant tumors. In the present study, routine blood tests were performed in order to observe the change of blood cells in tumor-bearing mice following the implantation of 4T1 breast cancer cells into the mammary fat pad; in addition, blood flow in breast tumor sites was measured indirectly using laser Doppler perfusion imaging (LDPI), in an attempt to explain the relevance between the blood circulation features and the growth or metastasis of breast cancer in mice model. The LDPI and blood test results indicated that the implantation of 4T1 breast cancer cells into BALB/c mice led to thrombosis as well as high WBC count, high platelet count, high plateletcrit and low blood perfusion. Following implantation of the 4T1 cells for four weeks, the lung metastatic number was determined and the Pearson correlation coefficient revealed that the number of visceral lung metastatic sites had a marked negative association with the ratio of basophils (BASO%; r=-0.512; P
- Published
- 2014
40. Inhibition of the Arp2/3 complex impairs early embryo development of porcine parthenotes
- Author
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Li, Ying-Hua, primary, Xu, Yong-Nan, additional, Lin, Zi-Li, additional, Kwon, JeongWoo, additional, Cui, Xiang-Shun, additional, and Kim, Nam-Hyung, additional
- Published
- 2016
- Full Text
- View/download PDF
41. miR-720 inhibits tumor invasion and migration in breast cancer by targeting TWIST1
- Author
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Shi Xun Lu, Jing Ping Yun, Lin Zi Li, Zhao Jie Zhang, Yi Han Peng, Li Li Liu, Chris Zhiyi Zhang, Xuan Zhou, Chun Yi, and Yuan Zhong Yang
- Subjects
Cancer Research ,Lung Neoplasms ,Cell ,Blotting, Western ,Mice, Nude ,Vimentin ,Apoptosis ,Breast Neoplasms ,Real-Time Polymerase Chain Reaction ,Metastasis ,Immunoenzyme Techniques ,Twist transcription factor ,Mice ,Breast cancer ,Downregulation and upregulation ,Cell Movement ,microRNA ,medicine ,Tumor Cells, Cultured ,Animals ,Humans ,Neoplasm Invasiveness ,RNA, Messenger ,Cell Proliferation ,Retrospective Studies ,Mice, Inbred BALB C ,biology ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,Twist-Related Protein 1 ,Cancer ,Nuclear Proteins ,General Medicine ,medicine.disease ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,medicine.anatomical_structure ,Lymphatic Metastasis ,Cancer research ,biology.protein ,Female ,business - Abstract
Breast cancer is the leading cause of cancer death among females, with tumor metastasis being primarily responsible for breast cancer-associated mortality. Current literatures have shown that microRNAs (miRNAs) are implicated in tumor metastasis. In this study, we found that the expression of miR-720 was significantly downregulated in primary breast cancer, with greater downregulation in metastatic tumors. Statistical analysis of 105 cases of primary human breast cancer demonstrated that decreased expression of miR-720 was correlated with lymph node metastasis. Furthermore, reexpression of miR-720 in breast cancer cells remarkably inhibited cell invasiveness and migration both in vitro and in vivo. Mechanistically, downregulation of TWIST1, a promoter of metastasis that was identified as a direct functional target of miR-720, was attributed to the inhibition of metastasis. Consistent with the reduced TWIST1 levels in breast cancer, reexpression of miR-720 upregulated epithelial markers (E-cadherin and β-catenin) and downregulated mesenchymal markers (N-cadherin, fibronectin, vimentin and matrix metalloproteinase-2). Expression of miR-720 was inversely associated with TWIST1 in human breast cancer tissues. Knockdown of TWIST1 expression by small interfering RNA exhibited similar effects to reintroduction of miR-720, whereas overexpression of TWIST1 (without the 3'-untranslated region) abrogated miR-720-mediated metastasis inhibition. Collectively, our data indicate that miR-720 is frequently decreased in breast cancer and manifests antimetastatic activity by downregulating TWIST1, presenting a novel mechanism of miRNA-mediated regulation of tumor metastasis.
- Published
- 2013
42. Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis
- Author
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Yan Li, Yan Huang, Mu Yan Cai, Chun Yi, Jing Ping Yun, Guo Bing Xie, Li Li Liu, Xuan Zhou, Chris Zhiyi Zhang, and Lin Zi Li
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Hepatocellular carcinoma ,Down-Regulation ,lcsh:Medicine ,AZGP1 ,Biology ,Real-Time Polymerase Chain Reaction ,Zn-Alpha-2-Glycoprotein ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Metastasis ,Downregulation and upregulation ,Recurrence ,medicine ,Carcinoma ,Humans ,RNA, Messenger ,DNA Primers ,Medicine(all) ,Base Sequence ,Biochemistry, Genetics and Molecular Biology(all) ,Research ,Liver Neoplasms ,lcsh:R ,Seminal Plasma Proteins ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Immunohistochemistry ,digestive system diseases ,Real-time polymerase chain reaction ,Tissue Array Analysis ,Cancer research ,Female ,Carcinogenesis - Abstract
Background Zinc-alpha2-glycoprotein (AZGP1, ZAG) was recently demonstrated to be an important factor in tumor carcinogenesis. However, AZGP1 expression in hepatocellular carcinoma (HCC) and its significance remain largely unknown. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to determine mRNA level of AZGP1 in 20 paired fresh HCC tissues. Clinical and pathological data of 246 HCC patients were collected. Tissue-microarray-based immunohistochemistry (IHC) was performed to examine AZGP1 expression in HCC samples. Relationship between AZGP1 expression and clinicopathological features was analyzed by Chi-square test, Kaplan-Meier analysis and Cox proportional hazards regression model. Results AZGP1 expression was significantly lower in 80.0% (16/20) of tumorous tissues than that in the corresponding adjacent nontumorous liver tissues (P P P = 0.013), liver cirrhosis (P = 0.002) and tumor differentiation (P = 0.025). Moreover, HCC patients with high AZGP1 expression survived longer, with better overall survival (P = 0.006) and disease-free survival (P = 0.025). In addition, low AZGP1 expression associated with worse relapse-free survival (P = 0.046) and distant metastatic progression-free survival (P = 0.036). Conclusion AZGP1 was downregulated in HCC and could be served as a promising prognostic marker for HCC patients.
- Published
- 2012
43. Distinct subcellular localization and potential role of LINE1-ORF1P in meiotic oocytes
- Author
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Luo, Yi-Bo, primary, Zhang, Li, additional, Lin, Zi-Li, additional, Ma, Jun-Yu, additional, Jia, Jialin, additional, Namgoong, Suk, additional, and Sun, Qing-Yuan, additional
- Published
- 2015
- Full Text
- View/download PDF
44. Computational Prediction of Alzheimer’s and Parkinson’s Disease MicroRNAs in Domestic Animals
- Author
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Wang, Hai Yang, primary, Lin, Zi Li, additional, Yu, Xian Feng, additional, Bao, Yuan, additional, Cui, Xiang-Shun, additional, and Kim, Nam-Hyung, additional
- Published
- 2015
- Full Text
- View/download PDF
45. Developing Water Pollution Accident Emergency Warning System for Chu River Downstream Basin
- Author
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Lin-zi Li, Yu Qian, and Yuchao Zhang
- Subjects
Water resources ,Geographic information system ,Warning system ,Downstream (manufacturing) ,business.industry ,Industrial park ,Environmental engineering ,Environmental science ,Water quality ,Water resource management ,business ,Water pollution ,Hazardous substance - Abstract
The occurrence of sudden water pollution accident threatens water quality as well as human health and social stabilization. A typical water pollution accident emergency warning system for Chu River downstream basin was developed to aid responsible authorities, water suppliers and emergency responders making impactful rescue measures at the first time in order to reduce influence and loss of the accident effectively. Consisted of the fundamental database, the GIS platform, and the surface water quality model WASP, the system realized the capability with near real-time simulation at temporal and spatial level following accident, toward the release for most of hazardous substance. The functionality of the system was illustrated through a scenario simulation where nitrobenzene assumed to be released accidentally from an enterprise in the Nanjing Chemical Industrial Park.
- Published
- 2010
46. Role of ataxia-telangiectasia mutated (ATM) in porcine oocyte in vitro maturation
- Author
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Lin, Zi-Li, primary and Kim, Nam-Hyung, additional
- Published
- 2015
- Full Text
- View/download PDF
47. Junction-mediating and regulatory protein (JMY) is essential for early porcine embryonic development
- Author
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LIN, Zi Li, primary, CUI, Xiang-Shun, additional, NAMGOONG, Suk, additional, and KIM, Nam-Hyung, additional
- Published
- 2015
- Full Text
- View/download PDF
48. Effect of anti-PMSG on distribution of estrogen receptor alpha and progesterone receptor in mouse ovary, oviduct and uterus
- Author
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Lin, Zi Li, primary, Ni, He Min, additional, Liu, Yun Hai, additional, Sheng, Xi Hui, additional, Cui, Xiang Shun, additional, Kim, Nam Hyung, additional, and Guo, Yong, additional
- Published
- 2014
- Full Text
- View/download PDF
49. The Association of Mitochondrial Potential and Copy Number with Pig Oocyte Maturation and Developmental Potential
- Author
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LEE, Seul-Ki, primary, ZHAO, Ming-Hui, additional, KWON, Jung-Woo, additional, LI, Ying-Hua, additional, LIN, Zi-Li, additional, JIN, Yong-Xun, additional, KIM, Nam-Hyung, additional, and CUI, Xiang-Shun, additional
- Published
- 2014
- Full Text
- View/download PDF
50. Association of CRP gene polymorphisms with CRP levels, frailty and co-morbidity in an elderly Chinese population: results from RuLAS.
- Author
-
ZU-YUN LIU, ZHENG-DONG WANG, LIN-ZI LI, XUE-FENG CHU, YIN-SHENG ZHU, JIAN-MING SHI, XUE-JUAN XIE, LI JIN, YONG WANG, and XIAO-FENG WANG
- Subjects
C-reactive protein ,CHI-squared test ,CONFIDENCE intervals ,FRAIL elderly ,GENETIC polymorphisms ,LONGITUDINAL method ,SCIENTIFIC observation ,RESEARCH funding ,DATA analysis software ,DESCRIPTIVE statistics ,ODDS ratio ,KRUSKAL-Wallis Test - Abstract
Objectives: to examine the associations of two common CRP gene polymorphisms with CRP levels, frailty and comorbidity in an elderly Chinese population. Design: a population-based cohort study. Setting and participants: we obtained data on 1,723 elderly participants aged 70-84 from the ageing arm of the Rugao Longevity and Ageing study (RuLAS), a population-based observational cohort study conducted in Rugao, Jiangsu province, China. Measurements: the genotyping of two common CRP gene polymorphisms (rs1205 and rs3093059) was performed. Items concerning the frailty index and comorbidity were collected. Results: the mean age of the study population was 75.3 ± 3.9 years, and 53.5% (n = 922) were women. The minor allele frequencies of rs1205 and rs3093059 were 42.4% (C allele) and 16.9% (C allele), respectively. The polymorphisms rs1205 and rs3093059 were significantly associated with CRP levels (β=0.113 and 0.222, all P< 0.001). Non-significant association between rs1205 and rs3093059 and frailty, as well as between rs3093059 and co-morbidity was observed. However, SNP rs1205 CC genotype had an increased odds of co-morbidity compared with the TT genotype (odds ratio (OR):1.53; 95% confidence interval (CI): 1.16--2.02). Each additional copy of the C allele of SNP rs1205 was associated with 1.23 times (95% CI: 1.07--1.41) odds of co-morbidity. The significance remained after controlling for covariates such as education level, etc. Conclusions: among elderly Chinese individuals, two CRP gene polymorphisms were significantly associated with CRP levels. However, none of them was associated with frailty. The preliminary findings warrant further validations. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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