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Your search keyword '"Levers, Marloes"' showing total 32 results
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32 results on '"Levers, Marloes"'

3. Hypothermia improves neuronal network recovery in a human-derived in vitro model of oxygen-deprivation.

Author

Voogd, Eva J. H. F., Thijs, Marloes, Levers, Marloes R., Hofmeijer, Jeannette, and Frega, Monica

Subjects
INDUCED pluripotent stem cells, NEURAL circuitry, CEREBRAL anoxia, THERAPEUTIC hypothermia, TISSUE arrays
Abstract

Mild therapeutic hypothermia showed potential neuroprotective properties during and after cerebral hypoxia or ischemia in experimental animal studies. However, in clinical trials, where hypothermia is mainly applied after reperfusion, results were divergent and neurophysiological effects unclear. In our current study, we employed human-derived neuronal networks to investigate how treatment with hypothermia during hypoxia influences neuronal functionality and whether it improves post-hypoxic recovery. We differentiated neuronal networks from human induced pluripotent stem cells on micro-electrode arrays (MEAs). We studied the effect of hypothermia (34°C)–as well hyperthermia (39°C) ‐ on neuronal functionality during and after hypoxia using MEAs. We also studied the effects on the number of synaptic puncta and cell viability by immunocytochemistry. In comparison to neuronal networks under normothermia, we found that hypothermia during hypoxia improved functional neuronal network recovery, expressed as enhanced neuronal network activity. This was associated with prevention of synaptic loss during and after the hypoxic phase. Furthermore, hypothermia improved cell viability after the hypoxic phase. Instead, hyperthermia during hypoxia had detrimental effects, with an irreversible loss of neuronal network function, loss of synaptic puncta and decreased cell viability. Our results show potential neuroprotective properties of hypothermia occurring during hypoxia, indicating that administering hypothermia to bridge the time to reperfusion may be beneficial in clinical settings. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Surface Topography-Mediated Monocyte Binding in vitro Is Not Predictive for the Foreign Body Response in vivo

Author

Diedrich, Chantal M., primary, Riool, Martijn, additional, Birgani, Zeinab Niloofar Tahmasebi, additional, Fekete, Natalie, additional, Konshin, Nikita, additional, Kuijpers, Tim, additional, Zhao, Yiping, additional, Hua, Wei-Shu, additional, Robinson, James, additional, Levers, Marloes R., additional, Vasilevich, Aliaksei, additional, de Boer, Leonie, additional, Florquin, Sandrine, additional, Roovers, Jan-Paul, additional, Papenburg, Bernke, additional, Zaat, Sebastian, additional, and de Boer, Jan, additional

Published
2024
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13. Neuroprotective effect of hypoxic preconditioning and neuronal activation in a in vitro human model of the ischemic penumbra

Author

Pires Monteiro, Sara, primary, Voogd, Eva, additional, Muzzi, Lorenzo, additional, De Vecchis, Gianmarco, additional, Mossink, Britt, additional, Levers, Marloes, additional, Hassink, Gerco, additional, Van Putten, Michel, additional, Le Feber, Joost, additional, Hofmeijer, Jeannette, additional, and Frega, Monica, additional

Published
2021
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14. Ischemic Stroke: Treatments to Improve Neuronal Functional Recovery in vitro

Author

Pires Monteiro, Sara, Covelo, Joana, Levers, Marloes, Hassink, Gerco, le Feber, Joost, Frega, Monica, Blobel, Bernd, Giacomini, Mauro, and Clinical Neurophysiology

Subjects
Ischemic stroke, rodent, iPSCs, neuronal network, penumbra, electrical stimulation
Abstract

The understanding of the neurophysiological processes that occur in the areas that surround the core of a brain infarct is crucial for the creation of new therapies and treatments to improve neuronal recovery. The present study aims to demonstrate that both rodent and human neuronal networks lose their activity under low oxygen conditions and that electrical stimulation can increase the probability of recovery. Hypoxia was induced in rodent and human neurons and the effects of electrical stimulation were assessed in the rat cultures. The results obtained show that neuronal activation, in the form of electrical stimulation, has the potential to maintain the networks at higher levels of activity and, therefore, to improve cell survival. This study will open the way for new treatment strategies based on brain-stimulation to enhance neuronal recovery and will be of large relevance for patients, families, and society.

Published
2019

18. Designed Surface Topographies Control ICAM-1 Expression in Tonsil-Derived Human Stromal Cells

Author

Vasilevich, Aliaksei S., primary, Mourcin, Frédéric, additional, Mentink, Anouk, additional, Hulshof, Frits, additional, Beijer, Nick, additional, Zhao, Yiping, additional, Levers, Marloes, additional, Papenburg, Bernke, additional, Singh, Shantanu, additional, Carpenter, Anne E., additional, Stamatialis, Dimitrios, additional, Blitterswijk, Clemens van, additional, Tarte, Karin, additional, and Boer, Jan de, additional

Published
2018
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19. Designed surface topographies control ICAM-1 expression in tonsil-derived human stromal cells

Author

Vasilevich, Aliaksei S., Mourcin, Frederic, Mentink, Anouk, Hulshof, Frits, Beijer, Nick, Zhao, Yiping, Levers, Marloes, Papenburg, Bernke, Singh, Shantanu, Carpenter, Anne E., Stamatialis, Dimitrios, van Blitterswijk, Clemens, Tarte, Karin, de Boer, Jan, Vasilevich, Aliaksei S., Mourcin, Frederic, Mentink, Anouk, Hulshof, Frits, Beijer, Nick, Zhao, Yiping, Levers, Marloes, Papenburg, Bernke, Singh, Shantanu, Carpenter, Anne E., Stamatialis, Dimitrios, van Blitterswijk, Clemens, Tarte, Karin, and de Boer, Jan

Abstract

Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in the lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known to be important for the initiation of immune responses, studies about FRCs and their interactions have been impeded because FRCs are limited in availability and lose their function upon culture expansion. To circumvent these limitations, stromal cell precursors can be mechanotranduced to form mature FRCs. Here, we used a library of designed surface topographies to trigger FRC differentiation from tonsil-derived stromal cells (TSCs). Undifferentiated TSCs were seeded on a TopoChip containing 2176 different topographies in culture medium without differentiation factors, then monitored cell morphology and the levels of ICAM-1, a marker of FRC differentiation. We identified 112 and 72 surfaces that upregulated and downregulated, respectively, ICAM-1 expression. By monitoring cell morphology, and expression of the FRC differentiation marker ICAM-1 via image analysis and machine learning, we discovered correlations between ICAM-1 expression, cell shape and design of surface topographies and confirmed our findings by using flow cytometry. Our findings confirmed that TSCs are mechano-responsive cells and identified particular topographies that can be used to improve FRC differentiation protocols.

Published
2018

21. Mining for osteogenic surface topographies: In silico design to in vivo osseo-integration

Author

Hulshof, Frits F. B., Hulshof, Frits F. B., Papenburg, Bernke, Vasilevich, Aliaksei, Hulsman, Marc, Zhao, Yiping, Levers, Marloes, Fekete, Natalie, de Boer, Meint, Yuan, Huipin, Singh, Shantanu, Beijer, Nick, Bray, Mark-Anthony, Reinders, Marcel, Carpenter, Anne E., van Blitterswijk, Clemens, Stamatialis, Dimitrios, de Boer, Jan, Logan, David J., Hulshof, Frits F. B., Hulshof, Frits F. B., Papenburg, Bernke, Vasilevich, Aliaksei, Hulsman, Marc, Zhao, Yiping, Levers, Marloes, Fekete, Natalie, de Boer, Meint, Yuan, Huipin, Singh, Shantanu, Beijer, Nick, Bray, Mark-Anthony, Reinders, Marcel, Carpenter, Anne E., van Blitterswijk, Clemens, Stamatialis, Dimitrios, de Boer, Jan, and Logan, David J.

Abstract

Stem cells respond to the physicochemical parameters of the substrate on which they grow. Quantitative material activity relationships - the relationships between substrate parameters and the phenotypes they induce - have so far poorly predicted the success of bioactive implant surfaces. In this report, we screened a library of randomly selected designed surface topographies for those inducing osteogenic differentiation of bone marrow-derived mesenchymal stem cells. Cell shape features, surface design parameters, and osteogenic marker expression were strongly correlated in vitro. Furthermore, the surfaces with the highest osteogenic potential in vitro also demonstrated their osteogenic effect in vivo: these indeed strongly enhanced bone bonding in a rabbit femur model. Our work shows that by giving stem cells specific physicochemical parameters through designed surface topographies, differentiation of these cells can be dictated. (C) 2017 Elsevier Ltd. All rights reserved.

Published
2017

23. Tumor suppressor BTG1 promotes PRMT1-mediated ATF4 function in response to cellular stress

Author

Yuniati, Laurensia, primary, van der Meer, Laurens T., additional, Tijchon, Esther, additional, van Ingen Schenau, Dorette, additional, van Emst, Liesbeth, additional, Levers, Marloes, additional, Palit, Sander A.L., additional, Rodenbach, Caroline, additional, Poelmans, Geert, additional, Hoogerbrugge, Peter M., additional, Shan, Jixiu, additional, Kilberg, Michael S., additional, Scheijen, Blanca, additional, and van Leeuwen, Frank N., additional

Published
2015
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24. A Germline Mutation in Cathepsin B in a Child with ALL Points towards a Key Role for This Enzyme in L-Asparaginase Pharmacokinetics.

Author

van der Meer, Laurens T., primary, Waanders, Esmé, additional, Levers, Marloes, additional, Venselaar, Hanka, additional, Roeleveld, Debbie, additional, Boos, Joachim, additional, Lanvers-Kaminsky, Claudia, additional, Kuiper, Roland P., additional, Hoogerbrugge, Peter M., additional, van Leeuwen, Frank N., additional, and te Loo, D. Maroeska W.M., additional

Published
2012
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27. BTG1, a Gene Frequently Deleted in Pre-B ALL, Controls Glucocorticoid Receptor-Mediated Gene Expression.

Author

van Leeuwen, Frank N., primary, van Galen, Joost Casper, additional, Kuiper, Roland P., additional, van Emst, Liesbeth, additional, Levers, Marloes R, additional, Tijchon, Esther, additional, Scheijen, Blanca, additional, Waanders, Esmee, additional, van Reijmersdal, Simon V, additional, Gillissen, Christian, additional, van Kessel, Ad Geurts, additional, and Hoogerbrugge, Peter M., additional

Published
2009
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31. Ischemic Stroke: Treatments to Improve Neuronal Functional Recovery in vitro.

Author

Pires Monteiro S, Covelo J, Levers M, Hassink G, le Feber J, and Frega M

Subjects
Animals, Humans, Hypoxia, Neurons, Rats, Brain Ischemia rehabilitation, Recovery of Function, Stroke
Abstract

The understanding of the neurophysiological processes that occur in the areas that surround the core of a brain infarct is crucial for the creation of new therapies and treatments to improve neuronal recovery. The present study aims to demonstrate that both rodent and human neuronal networks lose their activity under low oxygen conditions and that electrical stimulation can increase the probability of recovery. Hypoxia was induced in rodent and human neurons and the effects of electrical stimulation were assessed in the rat cultures. The results obtained show that neuronal activation, in the form of electrical stimulation, has the potential to maintain the networks at higher levels of activity and, therefore, to improve cell survival. This study will open the way for new treatment strategies based on brain-stimulation to enhance neuronal recovery and will be of large relevance for patients, families, and society.

Published
2019

32. Tumor suppressor BTG1 promotes PRMT1-mediated ATF4 function in response to cellular stress.

Author

Yuniati L, van der Meer LT, Tijchon E, van Ingen Schenau D, van Emst L, Levers M, Palit SA, Rodenbach C, Poelmans G, Hoogerbrugge PM, Shan J, Kilberg MS, Scheijen B, and van Leeuwen FN

Subjects
Animals, Apoptosis physiology, B-Lymphocytes cytology, Cell Line, Tumor, Fibroblasts, Humans, Methylation, Mice, Mice, Inbred C57BL, Mice, Knockout, Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics, Activating Transcription Factor 4 metabolism, Neoplasm Proteins metabolism, Precursor Cell Lymphoblastic Leukemia-Lymphoma pathology, Protein-Arginine N-Methyltransferases metabolism, Repressor Proteins metabolism, Stress, Physiological physiology
Abstract

Cancer cells are frequently exposed to physiological stress conditions such as hypoxia and nutrient limitation. Escape from stress-induced apoptosis is one of the mechanisms used by malignant cells to survive unfavorable conditions. B-cell Translocation Gene 1 (BTG1) is a tumor suppressor that is frequently deleted in acute lymphoblastic leukemia and recurrently mutated in diffuse large B cell lymphoma. Moreover, low BTG1 expression levels have been linked to poor outcome in several solid tumors. How loss of BTG1 function contributes to tumor progression is not well understood. Here, using Btg1 knockout mice, we demonstrate that loss of Btg1 provides a survival advantage to primary mouse embryonic fibroblasts (MEFs) under stress conditions. This pro-survival effect involves regulation of Activating Transcription Factor 4 (ATF4), a key mediator of cellular stress responses. We show that BTG1 interacts with ATF4 and positively modulates its activity by recruiting the protein arginine methyl transferase PRMT1 to methylate ATF4 on arginine residue 239. We further extend these findings to B-cell progenitors, by showing that loss of Btg1 expression enhances stress adaptation of mouse bone marrow-derived B cell progenitors. In conclusion, we have identified the BTG1/PRMT1 complex as a new modifier of ATF4 mediated stress responses.

Published
2016
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