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For the production of trans-resveratrol-amplified grape juice, both trans-resveratrol quantity and microbial quality are important for the functionality and shelf life of the juice. Therefore, the thermal stability of trans-resveratrol and thermal inactivation of Saccharomyces cerevisiae within the grape juice was investigated at 60°C, 80°C and 100°C. Inactivation of S. cerevisiae was fitted to first-order kinetics, and the inactivation rate (k1) and decimal reduction time (D-value) at each treatment temperature were estimated. The control grape juice had an inactivation rate of 0.0014 (s-1) and a D-value of 11.90 min at 60°C, whereas the trans-resveratrol-amplified grape juice had an inactivation rate of 0.0016 (s-1) and a Dvalue of 10.42 min at the same temperature. Similar inactivation kinetics of the control and trans-resveratrol-amplified grape juice were observed at 80°C and 100°C, which indicates that increased trans-resveratrol content does not affect the thermal inactivation of inoculated S. cerevisiae.

Abstract Background The diagnosis of dry eye and other common ophthalmological conditions can be supported using patient reported symptoms, which is increasingly useful in contexts such as telemedicine. We aim to ascertain visual symptoms that differentiate dry eye from cataract, glaucoma, or glaucoma suspects. Methods Adults with dry eye, glaucoma, cataract, and suspected glaucoma (controls) completed a questionnaire to rate the frequency and severity of 28 visual symptoms. Univariate, followed by multivariable logistic regression with backward stepwise selection (p

This study was designed to examine the role of early bilingual home literacy experiences (HLE) (including parent–child shared reading, parents’ direct teaching in Chinese and English, the availability of books in both languages, and children’s access to digital devices for bilingual learning) in the biliteracy development of 66 Chinese–Canadian first graders during the COVID-19 pandemic. Descriptive analyses reveal that overall, parents report higher engagement in English than in Chinese across the four HLE measures. Parent’s engagement in bilingual HLE differs by gender, SES, and immigration status. Pearson correlational analyses of English reading, decoding, and bilingual oral receptive vocabulary reveal that the four dimensions of HLE are not strongly related to English early literacy skills but are positively related to Chinese receptive vocabulary. Finally, hierarchical regression analyses indicate that the availability of books in Chinese and parent–child shared reading in Chinese are key factors associated with Chinese receptive vocabulary score variance; the amount of time using digital devices is found to be significantly related to English reading comprehension, but not Chinese vocabulary; and parents’ direct teaching is not significant with either English early literacy skills or Chinese receptive vocabulary. These findings have important implications for parental engagement in early bilingual home literacy activities and early literacy instruction in school.

Abstract Woody biomass is an important feedstock for biofuel production. Manipulation of wood properties that enable efficient conversion of biomass to biofuel reduces cost of biofuel production. Wood cell wall composition is regulated at several levels that involve expression of transcription factors such as wood‐/secondary cell wall‐associated NAC domains (WND or SND). In Arabidopsis thaliana, SND1 regulates cell wall composition through activation of its down‐stream targets such as MYBs. The functional aspects of SND1 homologs in the woody Populus have been studied through transgenic manipulation. In this study, we investigated the role of PdWND1B, Populus SND1 sequence ortholog, in wood formation using transgenic manipulation through over‐expression or silencing under the control of a vascular‐specific 4‐coumarate‐CoA ligase (4CL) promoter. As compared with control plants, PdWND1B‐RNAi plants were shorter in height, with significantly reduced stem diameter and dry biomass, whereas there were no significant differences in growth and productivity of PdWND1B over‐expression plants. Conversely, PdWND1B over‐expression lines showed a significant reduction in cellulose and increase in lignin content, whereas there was no significant impact on lignin content of downregulated lines. Stem carbohydrate composition analysis revealed a decrease in glucose, mannose, arabinose, and galactose, but an increase in xylose in the over‐expression lines. Transcriptome analysis revealed upregulation of several downstream transcription factors and secondary cell wall related structural genes in the PdWND1B over‐expression lines, partly explaining the observed phenotypic changes in cell wall chemistry. Relative to the control, glucose release efficiency and ethanol production from stem biomass was significantly reduced in over‐expression lines. Our results show that PdWND1B is an important factor determining biomass productivity, cell wall chemistry and its conversion to biofuels in Populus.

The guidelines recommend radiofrequency ablation (RFA) for early hepatocellular carcinomas that are less than 3 cm and trans-arterial chemoembolization (TACE) for intermediate-stage tumors. Real-world patient and tumor factors commonly limit strict adherence to the guidelines. We aimed to compare the clinical outcomes for TACE and RFA in early HCC. All consecutive patients from 2010 to 2014 that were treated with locoregional therapy at our institution were enrolled. The decision for TACE or RFA was based on tumor location, stage and technical accessibility for ablation. A subgroup analysis was performed for patients with tumors less than 3 cm. A total of 168 patients underwent TACE while 56 patients underwent RFA. Patients treated with TACE and RFA had 1- and 5-year survival rates of 84.7% and 39.8% versus 91.5% and 51.5%, respectively (p = 0.28). In tumors less than 3 cm, there was no significant difference in overall survival (p = 0.69), time to progression (p = 0.55), or number of treatment sessions required (p = 0.12). Radiofrequency ablation had a significantly higher chance of a complete response (p = 0.004). In conclusion, TACE may be selectively considered for early-stage hepatocellular carcinoma in patients unsuitable for other modalities.

Metasurfaces have received enormous attention thanks to their unique ability to modulate electromagnetic properties of light in various frequency regimes. Recently, exploiting its fabrication ease and modulation strength, unprecedented and unique controlling of light that surpasses conventional optical devices has been suggested and studied a lot. Here, in this paper, we discuss some parts of this trend including holography, imaging application, dispersion control, and multiplexing, mostly operating for optical frequency regime. Finally, we will outlook the future of the devices with recent applications of these metasurfaces.

Plant drought stress causes systematic changes to photosynthesis, metabolism, growth, and potentially the phytobiome. Additionally, drought affects plants in both a species-specific and water-deficit-driven manner, causing the response to drought to be dependent both on how drought is being experienced and on any adaptation to prior drought exposure. Thus, understanding the effect of drought on plants requires assessing drought response in multiple conditions, such as progressive acute drought and recurrent cyclic drought, and at different levels of severity. In this study, we have utilized RNA sequencing to identify changes to the plant transcriptome and the phytobiome during both acute progressive drought and cyclic drought at multiple severities. Co-analysis of the plant and phytobiome, utilizing the same RNAseq data, allows for the identification of novel associations that would not be possible otherwise. We have identified that the drought response ranges from increased transcripts related to photosynthesis and metabolic activity in mild acute drought to decreased transcripts related to photosynthesis and metabolic impairment in severe drought. Moreover, while water deficit is a main driver of transcriptional responses in severe drought, there are increases in reactive oxygen species (ROS) metabolism and photosynthetic transcripts in cyclic severe drought compared with acute severe drought, independent of water deficit. The phytobiome exhibits alternate responses to drought when compared with the transcriptome. Specifically, the phytobiome is affected more by the cyclic or acute nature of the drought rather than the severity of the drought, with the phytobiome having an increase in taxa under cyclic drought that are often reported to have beneficial effects on the plants. Lastly, we have identified associations between taxa in the phytobiome with expression of disease response, ROS metabolism, and photosynthesis transcripts suggesting interplay between the host plant and its phytobiome in response to drought.

Since 2006, retired U.S. admirals and generals have been examining our changing physical world and assessing the impact of those changes on the security of the nation. A Military Advisory Board (MAB, convened by the CNA Corporation, a non-profit research and analysis institution that operates the Center for Naval Analyses and Institute for Public Research) has issued two reports on the changing climate’s national security implications. The first report, published in 2007, stated that the changing climate would be destabilizing in many parts of the world. Climate change, in fact, would be a “threat multiplier”, the report claimed, and result in new and more urgent calls for the American military to provide humanitarian assistance and disaster relief (HADR) as well as help sustain order and ensure conditions that would permit trade and prosperity worldwide. The MAB recommended urgent action by the Defense Department to prepare for new missions in new places; and that the Defense Department explicitly recognize missions stemming from the changing climate. CNA’s MAB then published three reports on energy, relating to climate change, one each on: the coming age of renewables, the nation’s energy dependence, and the future of energy in terms of America’s international competitiveness (CNA-MAB, 2009; CNA-MAB, 2010; CNA-MAB, 2011). In 2014, the Military Advisory Board noted that the climate was changing more quickly than had been forecast in 2007. Undertaking a new climate study, the board concluded that more needed to be done, and done quickly, to prepare for and confront the “catalyst for conflict” that the board now saw in climate change. Guest Editors’ Note: The following commentary derives from a presentation by Vice Admiral Lee Gunn, U.S. Navy (Ret.), delivered by invitation at the American Association for the Advancement of Science Meeting, San Jose, California, February 13, 2015. Prior to his advisory role for the U.S. Military and national security community with the CNA Corporation (https://www.cna.org), he served for 35 years in the U.S. Navy. His last active duty assignment was Inspector General of the Department of the Navy where he was responsible for the Department’s overall inspection program and its assessments of readiness, training, and quality of service. Serving in the Surface Navy in a variety of theaters, Gunn rose through the cruiser/destroyer force to command the Frigate USS Barbey, then commanded the Navy’s anti-submarine warfare tactical and technical evaluation Destroyer squadron, DESRON 31. He later commanded Amphibious Group Three. As Commander of PHIBGRU THREE, he served as the Combined Naval Forces Commander and Deputy Task Force Commander of Combined Task Force United Shield, which conducted the withdrawal of U.N. peacekeeping forces from Somalia. Since retiring from active duty in the Navy, Gunn has been President of The Institute for Public Research at CNA Corporation. Gunn holds a Bachelor’s degree in Experimental and Physiological Psychology from the University of California, Los Angeles and a Master of Science in Operations Research from the Naval Postgraduate School in Monterey, California. His commentary addresses why members of the national security community regard climate change as a clear threat to national security now, and a threat that will only increase if climate change continues unchecked.

Objective: The advent and utility of new endoscopic imaging modalities for predicting the histology of Barrett’s esophagus (BE) in real time with high accuracy appear promising and could potentially obviate the need to perform random biopsies where guidelines are poorly adhered to. We embarked on evaluating the performance characteristics of white-light endoscopy with magnification (WLE-z), narrow-band imaging with magnification (NBI-z) and a combination of both modalities. Design: This was a prospective online study with 28 endoscopists from 11 countries (Asia-Pacific region) participating as assessors. In total, 35 patients with BE were assessed using 150 slides from WLE-z and NBI-z randomly arranged using a simple classification with corresponding histology. The overall Accuracy (Acc), Sensitivity (Sn), Specificity (Sp), Positive Predictive Value (PPV), and Negative Predictive Value (NPV) of WLE-z, NBI-z and a combination of both were calculated. Results: The overall Acc for WLE-z and NBI-z images was 87.1 % and 88.7 %, respectively. When images from the two modalities were placed side by side, the Acc increased to 90.3 %. The Sn, Sp, PPV, and NPV of WLE-z were 48 %, 92 %, 45 %, and 93 % while with NBI-z, these improved to 89 %, 89 %, 56 %, and 98 %, respectively. When both imaging modalities were viewed together, they improved further to 93 %, 90 %, 61 %, and 99 %. Conclusion: The high NPV (99 %) when both WLE-z and NBI-z were used simultaneously indicates that areas with regular appearance that are diagnosed with confidence can effectively be left alone and not biopsied when performed at a skilled resourced center. This approach could potentially lead to a paradigm shift of how patients with BE are assessed.

Objective : This study is perfonned for the purpose of examining the efficacy of the scolopendrid (Scolopendra morsitans L.) which has been used to low back pain as a folk remedy. Methods & Results : Seventy patients with H.I.V.D. treated with the scolopendrid aquacupuncture and examined 4 tests; D.I.T.L., physical test, Oswestry disability index, and self-conscious pain rate were studied. There was 50~100% of efficacy rate in each tests. Moreover, statistically valuable change in 5 to 7 D.I.T.I. points and in self-conscious pain rate was showed. Conclusion : The scolopendrid aquacupuncture is possible to be effective treating the H.I.V.D patients. So we suggest the possibility using this new remedy to the H.I.V.D. patients.

Bacterial and fungal communities associated with plant roots are central to the host health, survival and growth. However, a robust understanding of the root-microbiome and the factors that drive host associated microbial community structure have remained elusive, especially in mature perennial plants from natural settings. Here, we investigated relationships of bacterial and fungal communities in the rhizosphere and root endosphere of the riparian tree species Populus deltoides, and the influence of soil parameters, environmental properties (host phenotype and aboveground environmental settings), host plant genotype (Simple Sequence Repeat (SSR) markers), season (Spring vs. Fall) and geographic setting (at scales from regional watersheds to local riparian zones) on microbial community structure. Each of the trees sampled displayed unique aspects to its associated community structure with high numbers of Operational Taxonomic Units (OTUs) specific to an individual trees (bacteria >90%, fungi >60%). Over the diverse conditions surveyed only a small number of OTUs were common to all samples within rhizosphere (35 bacterial and 4 fungal) and endosphere (1 bacterial and 1 fungal) microbiomes. As expected, Proteobacteria and Ascomycota were dominant in root communities (>50%) while other higher-level phylogenetic groups (Chytridiomycota, Acidobacteria) displayed greatly reduced abundance in endosphere compared to the rhizosphere. Variance partitioning partially explained differences in microbiome composition between all sampled roots on the basis of seasonal and soil properties (4% to 23%). While most variation remains unattributed, we observed significant differences in the microbiota between watersheds (Tennessee vs. North Carolina) and seasons (Spring vs. Fall). SSR markers clearly delineated two host populations associated with the samples taken in TN vs. NC, but overall host genotypic distances did not have a significant effect on corresponding communities that could be separated from other measured effects.

In this study, we established a comprehensive genetic map with a large number of progeny from a three-generation hybrid Populus intercross, and phenotyped the lignin content, S/G ratio and 28 cell wall subcomponents both in stems and roots for the mapping individuals. Phenotypic analysis revealed that lignin content and syringyl-to-guaiacyl (S/G) ratio using pyrolysis molecular beam mass spectroscopy (pyMBMS) varied among mapping individuals. Phenotypic analysis revealed that stem lignin content is significantly higher than that in root and the quantified traits can be classified into four distinct groups, with strong correlations observed among components within organs. Altogether, 179 coordinating QTLs were detected, and they were co-localized into 49 genetic loci, 27 of which appear to be pleiotropic. Many of the detected genetic loci were detected differentially in stem and root. This is the first report of separate genetic loci controlling cell wall phenotypes above and below ground. These results suggest that it may be possible to modify lignin content and composition via breed and/or engineer as a means of simultaneously improving Populus for cellulosic ethanol production and carbon sequestration.

Abstract A multiobjective fractional optimization problem (MFP), which consists of more than two fractional objective functions with convex numerator functions and convex denominator functions, finitely many convex constraint functions, and a geometric constraint set, is considered. Using parametric approach, we transform the problem (MFP) into the non-fractional multiobjective convex optimization problem (NMCP) v with parametric v ∈ ℝ p , and then give the equivalent relation between (weakly) ε-efficient solution of (MFP) and (weakly) -efficient solution of . Using the equivalent relations, we obtain ε-optimality conditions for (weakly) ε-efficient solution for (MFP). Furthermore, we present examples illustrating the main results of this study. 2000 Mathematics Subject Classification: 90C30, 90C46.