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131 results on '"Lecourieux, Fatma"'

9. Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells

Author

Kong, Junhua, primary, Garcia, Virginie, additional, Zehraoui, Enric, additional, Stammitti, Linda, additional, Hilbert, Ghislaine, additional, Renaud, Christel, additional, Maury, Stéphane, additional, Delaunay, Alain, additional, Cluzet, Stéphanie, additional, Lecourieux, Fatma, additional, Lecourieux, David, additional, Teyssier, Emeline, additional, and Gallusci, Philippe, additional

Published
2022
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11. The state-of-the-art of grapevine biotechnology and new breeding technologies (NBTS)

Author

Lecourieux, Fatma, Dalla Costa, Lorenza, Malnoy, Mickael, Lecourieux, David, Deluc, Laurent, Ouaked- Lecourieux, Fatma, Thomas, Mark, Torregrosa, Laurent Jean-Marie, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB), Fdn E Mach, Ist Agr San Michele allAdige, Génétique, Reproduction et Développement - Clermont Auvergne (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), and OUAKED-LECOURIEUX, Fatma

Subjects
Vine, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Gene Transfer, Genetic Improvement, Context (language use), 02 engineering and technology, Biotechnologies, Horticulture, Biology, Genome, lcsh:Agriculture, Genome editing, lcsh:Botany, 0202 electrical engineering, electronic engineering, information engineering, Genome Editing, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Selection (genetic algorithm), ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, Classical genetics, business.industry, lcsh:S, food and beverages, 021001 nanoscience & nanotechnology, lcsh:QK1-989, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, Genetically modified organism, Biotechnology, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, Settore AGR/07 - GENETICA AGRARIA, grapevine, biotechnologies, gene transfer, genome editing, genetic improvement, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, 020201 artificial intelligence & image processing, Grapevine, Adaptation, 0210 nano-technology, business, Food Science
Abstract

International audience; Context of the review: The manipulation of the genetic basis controlling grapevine adaptation and phenotypic plasticity can be performed either by classical genetics or biotechnologies. In the last 15 years, considerable knowledge has accumulated about the grapevine genome as well as the mechanisms involved in the interaction of the vine with the environment, pests and diseases. Despite the difficulties associated with genetic mapping in this species (allele diversity, chimerism, long generation intervals.), several major QTLs (quantitative trait loci) controlling important vegetative or reproductive traits have been identified. Considering the huge genotypic and phenotypic diversities existing in Vitis, breeding offers a substantial range of options to improve the performances of cultivars. However, even if marker-assisted selection was largely developed to shorten breeding programs, the selection of improved cultivars, whether for agronomic traits or disease tolerances, is still long and uncertain. Moreover, breeding by crossing does not preserve cultivar genetic background, when the wine industry and market are still based on varietal wines. Significance of the review: In grapevine, pioneering biotechnologies were set up in the 1960s to propagate and/or clean the material from micro-organisms. In the 1990s, the basis of genetic engineering was primary established through biolistic or Agrobacterium with several derived technologies refined in the last 10 years. The latest advance is represented by a group of technologies based on genome editing which allows a much more precise modification of the genome. These technologies, so-called NBTs (new breeding technologies), which theoretically do not deconstruct the phenotype of existing cultivars, could be potentially better accepted by the wine industry and consumers than previous GMO (genetically modified organism) approaches. This paper reviews the current state-of-the-art of the biotechnologies available for grapevine genome manipulation and future prospects for genetic improvement.

Published
2019
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14. Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola

Author

Liu, Guo-Tian, Wang, Bian-Bian, Lecourieux, David, Li, Mei-Jie, Liu, Ming-Bo, Liu, Rui-Qi, Shang, Bo-Xing, Yin, Xiao, Wang, Li-Jun, Lecourieux, Fatma, Xu, Yan, Northwest A and F University, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Chinese Academy of Sciences [Beijing] (CAS)

Subjects
Proteomics, [SDE]Environmental Sciences, Biotic, Article
Abstract

International audience; Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii "LiuBa-8" (LB) and susceptible V. vinifera "Pinot Noir" (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN, respectively. The majority of these DEPs were related to photosynthesis, respiration, cell wall modification, protein metabolism, stress, and redox homeostasis. Compared with PN, LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism. At least a subset of PR proteins (PR10.2 and PR10.3) was upregulated upon inoculation in both genotypes, whereas HSP (HSP70.2 and HSP90.6) and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN, respectively. In the incompatible interaction, ROS signaling was evident by the accumulation of H 2 O 2 , and multiple APX and GST proteins were upregulated. These DEPs may play crucial roles in the grapevine response to downy mildew. Our results provide new insights into molecular events associated with downy mildew resistance in grapevine, which may be exploited to develop novel protection strategies against this disease.

Published
2020
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18. Additional file 1 of Knockout of VvCCD8 gene in grapevine affects shoot branching

Author

Ren, Chong, Yuchen Guo, Junhua Kong, Lecourieux, Fatma, Zhanwu Dai, Shaohua Li, and Zhenchang Liang

Abstract

Additional file 1: Figure S1. Sequencing results of VvCCD7 and VvCCD8 fragments amplified from 41B cells. a The target sequence of VvCCD7 gene in 41B. b The target sequence of VvCCD8 gene in 41B. The target sites in VvCCD7 and VvCCD8 genes are highlighted in dark blue.

Published
2020
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19. Additional file 2 of Knockout of VvCCD8 gene in grapevine affects shoot branching

Author

Ren, Chong, Yuchen Guo, Junhua Kong, Lecourieux, Fatma, Zhanwu Dai, Shaohua Li, and Zhenchang Liang

Abstract

Additional file 2: Figure S2. Sequencing results of the two putative off-target sites in VvCCD8 knockout lines. The two off-target sites predicted within exons of other genes were selected for off-target analysis. Two VvCCD8 knockout lines, Plant #1 and Plant #6 were used in the experiment. The amplified fragments containing the off-target sites were amplified and cloned into pLB-Simple vector. At least 6 clones for each site were used for Sanger sequencing.

Published
2020
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20. Additional file 3 of Knockout of VvCCD8 gene in grapevine affects shoot branching

Author

Ren, Chong, Yuchen Guo, Junhua Kong, Lecourieux, Fatma, Zhanwu Dai, Shaohua Li, and Zhenchang Liang

Subjects
food and beverages
Abstract

Additional file 3: Figure S3. The original gel image of PCR identification of T-DNA insertions in CCD8-sgRNA plants. The vector plasmid (P1) and the transgenic cells (P2) were used as the positive controls, while wild-type plant was used as the negative control (N). Lanes 1–6 represent individual CCD8-sgRNA plants.

Published
2020
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22. Involvement of a heat-inducible ERF in the control of the sugar/acid balance in grape berries

Author

Lecourieux, David, GOMES, Eric, Moreau, Hortense, Delrot, Serge, Gaillard, Isabelle, Lecourieux, Fatma, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), and Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

23. Characterization of Chromatin Accessibility and Gene Expression upon Cold Stress Reveals that the RAV1 Transcription Factor Functions in Cold Response in Vitis Amurensis.

Author

Ren, Chong, Li, Huayang, Wang, Zemin, Dai, Zhanwu, Lecourieux, Fatma, Kuang, Yangfu, Xin, Haiping, Li, Shaohua, and Liang, Zhenchang

Subjects
GENE expression, TRANSCRIPTION factors, GRAPES, GENE regulatory networks, PLANT cell walls, PHYSIOLOGICAL effects of cold temperatures
Abstract

Cold tolerance is regulated by a variety of transcription factors (TFs) and their target genes. Except for the well-characterized C-repeat binding factors (CBFs)-dependent transcriptional cascade, the mechanisms of cold tolerance mediated by other transcriptional regulatory networks are still largely unknown. Here, we used the assay for transposase-accessible chromatin with sequencing (ATAC-seq) and RNA-seq to identify cold responsive TFs in Vitis amurensis , a grape species with high cold hardiness. Nine TFs, including CBF4, RAV1 and ERF104, were identified after cold treatment. Weighted gene co-expression network analysis (WGCNA) and gene ontology (GO) analysis revealed that these TFs may regulate cold response through different pathways. As a prime candidate TF, overexpression of VaRAV1 in grape cells improved its cold tolerance. The transgenic cells exhibited low electrolyte leakage and malondialdehyde content and high peroxidase activity. Moreover, the TF gene TCP8 and a gene involving in homogalacturonan biosynthesis were found to be regulated by VaRAV1, suggesting that the contribution of VaRAV1 to cold tolerance may be achieved by enhancing the stability of cell membrane and regulating the expression of target genes involved in plant cell wall composition. Our work provides novel insights into plant response to cold stress and demonstrates the utility of ATAC-seq and RNA-seq for the rapid identification of TFs in response to cold stress in grapevine. VaRAV1 may play an important role in adaption to cold stress. [ABSTRACT FROM AUTHOR]

Published
2021
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24. Proteomic and metabolomic profiling underlines the stage‐ and time‐dependent effects of high temperature on grape berry metabolism

Author

Lecourieux, David, primary, Kappel, Christian, additional, Claverol, Stéphane, additional, Pieri, Philippe, additional, Feil, Regina, additional, Lunn, John E., additional, Bonneu, Marc, additional, Wang, Lijun, additional, Gomès, Eric, additional, Delrot, Serge, additional, and Lecourieux, Fatma, additional

Published
2020
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25. Functional characterization of a heat-inducible ethylene response factor and its putative role in the control of the sugar/acid balance in grape berries

Author

Lecourieux, David, Delrot, Serge, MOREAU, Hortense, Gaillard, Isabelle, Gomes, Eric, Lecourieux, Fatma, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

26. DNA methylation regulates anthocyanin accumulation in the grape cell suspension

Author

KONG, Junhua, Teyssier, Emeline, Lecourieux, David, Gallusci, Philippe, Lecourieux, Fatma, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

27. Grapevine adaptation to abiotic stresses: an overview

Author

Lecourieux, Fatma, Ollat, N., Cookson, S.J., Destrac-Irvine, A., Lauvergeat, V., Ouaked-Lecourieux, F., Marguerit, E., Barrieu, F., Dai, Z., Duchêne, E., Gambetta, G.A., Gomès, E., Lecourieux, D., Van Leeuwen, C., Simonneau, T., Torregrosa, L., Vivin, P., Delrot, S., Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB), Centre National de la Recherche Scientifique (CNRS), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Shandong University, UMR5205, Université de Lyon, Université de Strasbourg (UNISTRA)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and ProdInra, Migration

Subjects
[SDE] Environmental Sciences, 0106 biological sciences, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, abiotic stress, [SDV]Life Sciences [q-bio], [SDE.MCG]Environmental Sciences/Global Changes, drought, Horticulture, Biology, 01 natural sciences, Genome, phenology, heat stress, 03 medical and health sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Allele, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 2. Zero hunger, Abiotic component, 0303 health sciences, Abiotic stress, fungi, food and beverages, Interspecific competition, berry composition, 15. Life on land, rootstocks, [SDV] Life Sciences [q-bio], Evolutionary biology, [SDE]Environmental Sciences, Sustainability, Adaptation, Genetic adaptation, 010606 plant biology & botany
Abstract

International audience; Adaptation to abiotic stresses is a complex and challenging biological issue for a genetician, especially for perennial plants such as grapevine. According to Copper and Hammer (1996), adaptation is both a “status” and a “process”. The “status” characterizes a genotype with a specific combination of alleles which allows the plant to survive and perform well in a specific environment. The adapted phenotype may result from a constitutive expression of the genotype or from genotype x environment interactions, in other words from the plasticity of traits. This modification of phenotype is referred to as acclimation. Physiological changes underlying acclimation occur over the short-term or over the life-cycle length of the individual. The “process” of adaptation will result from the new combination of alleles over generations to obtain a phenotype better able to survive or grow under abiotic constraints. By definition, the “process” time-scale is the reproductive cycle length. In addition for a crop, an adapted phenotype will include survival, yield maintenance, and especially for grapevine, optimal fruit composition. Considering the economic importance of grapevine, the ongoing and expected climate changes make the issue of adaptation even more challenging. Given the numerous environments where this plant can be found and the huge intra and interspecific diversity, we can assume that the grapevine genome bears many alleles which could be mobilized to “adapt” this crop and maintain its sustainability. The challenge is to identify these alleles and understand how they can be leveraged in manipulating the phenotype. The diversity of abiotic constraints (thermal stress, drought, salinity, mineral deficiency, etc...) and their characteristics in terms of their timing, duration, and intensity need to be taken into account. On the plant side, the traits underlying adaptation and the stage of sensitivity should be clearly defined. Targeted traits are often complex and under the control of various genetic mechanisms. Over the past ten years, there have been numerous achievements in grapevine in genome sequencing, phenotyping, genetic architecture analyses, gene identification, and modeling. Thanks to this new knowledge and technologies, our understanding of adaptation to abiotic stresses has improved and can now be used to screen for particular behaviors in existing germplasms or to breed new ones. An overview of the work performed in France over the past years aimed at adaptation to temperature and drought will be presented.

Published
2018
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30. Purification and functional characterization of protoplasts and intact vacuoles from grape cells

Author

Gerós Hernâni, Lecourieux Fatma, Vignault Céline, Silva Rui, Fontes Natacha, and Delrot Serge

Subjects
Medicine, Biology (General), QH301-705.5, Science (General), Q1-390
Abstract

Abstract Background During grape berry ripening, the vacuoles accumulate water, sugars and secondary metabolites, causing great impact in plant productivity and wine quality. However, the molecular basis of these compartmentation processes is still poorly understood. As in many species, the major bottleneck to study these aspects in grapevine is to obtain highly purified vacuoles with a good yield. The present paper describes an isolation method of protoplasts and intact vacuoles from grape berry cells and their functional characterization by transport and cytometric assays. Findings Protoplasts were prepared by enzymatic digestion of grape cells, and vacuoles were released and purified by a Ficoll step gradient centrifugation. The tonoplast stained strongly with the fluorescent dye FM1-43 and most vacuoles maintained an internal acidic pH, as assessed by Neutral Red. Flow cytometry analysis of vacuole samples incubated with the calcium-sensitive fluorescent probe Fluo-4 AM revealed a well-defined sub-population of intact vacuoles. As assessed by the pH-sensitive probe ACMA, intact vacuoles generated and maintained a pH gradient through the activity of V-ATPase and V-PPase and were able to transport Ca2+ via a proton-dependent transport system. Conclusions Highly pure, intact and functional protoplast and vacuole populations from grape cells were obtained with the present method, which revealed to be fast and efficient. The capacity of the vacuole population to sequester protons and accumulate Ca2+ strongly suggests the intactness and physiological integrity of these extremely fragile organelles. Grapevine protoplasts and vacuoles may be used as models for both basic research and biotechnological approaches, such as proteomics, solute uptake and compartmentation, toxicological assessments and breeding programs.

Published
2010
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32. Dissecting the Biochemical and Transcriptomic Effects of a Locally Applied Heat Treatment on Developing Cabernet Sauvignon Grape Berries

Author

Lecourieux, Fatma, Kappel, Christian, Pieri, Philippe, Charon, Justine, Pillet, Jérémy, Hilbert, Ghislaine, Renaud, Christel, Gomès, Eric, Delrot, Serge, Lecourieux, David, Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), and Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)

Subjects
microclimat, Biodiversité et Ecologie, [SDV]Life Sciences [q-bio], metabolomics/metabolite profiling, Plant Science, berry development, climate change, grapevine, high temperature, microarrays, microclimate, baie de raisin, Biodiversity and Ecology, vitis vinifera, ddc:570, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Institut für Biochemie und Biologie, ComputingMilieux_MISCELLANEOUS, global change, Original Research, changement climatique, food and beverages, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, vigne, traitement thermique
Abstract

International audience; Reproductive development of grapevine and berry composition are both strongly influenced by temperature. To date, the molecular mechanisms involved in grapevine berries response to high temperatures are poorly understood. Unlike recent data that addressed the effects on berry development of elevated temperatures applied at the whole plant level, the present work particularly focuses on the fruit responses triggered by direct exposure to heat treatment (HT). In the context of climate change, this work focusing on temperature effect at the microclimate level is of particular interest as it can help to better understand the consequences of leaf removal (a common viticultural practice) on berry development. HT (+8 degrees C) was locally applied to clusters from Cabernet Sauvignon fruiting cuttings at three different developmental stages (middle green, veraison and middle ripening). Samples were collected 1, 7, and 14 days after treatment and used for metabolic and transcriptomic analyses. The results showed dramatic and specific biochemical and transcriptomic changes in heat exposed berries, depending on the developmental stage and the stress duration. When applied at the herbaceous stage, HT delayed the onset of veraison. Heating also strongly altered the berry concentration of amino acids and organic acids (e.g., phenylalanine, raminobutyric acid and malate) and decreased the anthocyanin content at maturity. These physiological alterations could be partly explained by the deep remodeling of transcriptome in heated berries. More than 7000 genes were deregulated in at least one of the nine experimental conditions. The most affected processes belong to the categories "stress responses," protein metabolism" and "secondary metabolism," highlighting the intrinsic capacity of grape berries to perceive HT and to build adaptive responses. Additionally, important changes in processes related to "transport," "hormone" and "cell wall" might contribute to the postponing of veraison. Finally, opposite effects depending on heating duration were observed for genes encoding enzymes of the general phenylpropanoid pathway, suggesting that the HI induced decrease in anthocyanin content may result from a combination of transcript abundance and product degradation.

Published
2017
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33. Grapevine adaptation to climate warning: new ways to breed varieties better adapted to elevated temperature

Author

Torregrosa, Laurent, Bigard, Antoine, Doligez, Agnes, Lecourieux, David, RIENTH, Markus, Luchaire, Nathalie, Pieri, Philippe, Chatbanyong, Ratthaphon, SHAHOOD, Resk, Farnos, Marc, Roux, Catherine, Adiveze, Angelique, Pillet, Jérémy, SIRE, Yannick, Zumstein, Emmanuelle, Veyret, Mélanie, Le Cunff, Loïc, Lecourieux, Fatma, Saurin, Nicolas, Muller, Bertrand, Ojeda, Hernan, Houel, Cléa, Peros, Jean-Pierre, This, Patrice, Pellegrino, Anne, Romieu, Charles, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Unité expérimentale de Pech-Rouge (PECH ROUGE), Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

34. Direct impact of high temperatures on grapevine berry development: a merge transcriptomic, proteomic and metabolomic survey

Author

LECOURIEUX, Fatma, Kappel, C., Pieri, Philippe, Charon, Justine, Pillet, Jérémy, Claverol, S., Lunn, J., Hilbert, Ghislaine, Renaud, Christel, Bonneu, Marc, GOMES, Eric, Delrot, Serge, Lecourieux, David, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), University of Potsdam, Université de Bordeaux Ségalen [Bordeaux 2], Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, and Institut National de Recherche Agronomique (INRA). UMR Ecophysiologie et Génomique Fonctionnelle de la Vigne (1287).

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

35. Developmental, molecular and genetic studies on grapevine response to temperature open breeding strategies for adaptation to warming

Author

Torregrosa, Laurent, primary, Bigard, Antoine, additional, Doligez, Agnes, additional, Lecourieux, David, additional, Rienth, Markus, additional, Luchaire, Nathalie, additional, Pieri, Philippe, additional, Chatbanyong, Ratthaphon, additional, Shahood, Rezth, additional, Farnos, Marc, additional, Roux, Catherine, additional, Adiveze, Angélique, additional, Pillet, Jérémie, additional, Sire, Yannick, additional, Zumstein, Emmanuelle, additional, Veyret, Mélanie, additional, Le Cunff, Loic, additional, Lecourieux, Fatma, additional, Saurin, Nicolas, additional, Muller, Bertrand, additional, Ojeda, Hernán, additional, Houel, Cléa, additional, Péros, Jean-Pierre, additional, This, Patrice, additional, Pellegrino, Anne, additional, and Romieu, Charles, additional

Published
2017
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36. VvGOLS1 and VvHsfA2 are Involved in the Heat Stress Responses in Grapevine Berries

Author

Pillet, Jérémy, Egert, Aurélie, Pieri, Philippe, Lecourieux, Fatma, Kappel, Christian, Charon, Justine, Gomès, Eric, Keller, Felix, Delrot, Serge, Lecourieux, David, Pillet, Jérémy, Egert, Aurélie, Pieri, Philippe, Lecourieux, Fatma, Kappel, Christian, Charon, Justine, Gomès, Eric, Keller, Felix, Delrot, Serge, and Lecourieux, David

Abstract

Among various environmental factors, temperature is a major regulator affecting plant growth, development and fruit composition. Grapevine is the most cultivated fruit plant throughout the world, and grapes are used for wine production and human consumption. The molecular mechanisms involved in grapevine tolerance to high temperature, especially at the fruit level, are poorly understood. To better characterize the sensitivity of berries to the microenvironment, high temperature conditions were locally applied to Vitis vinifera Cabernet Sauvignon clusters. Two genes, VvGOLS1 and VvHsfA2, up-regulated by this treatment, were identified and further characterized. The expression profile of VvGOLS1 correlated positively with galactinol accumulation in heat-stressed berries. However, no galactinol derivatives, such as raffinose and stachyose, accumulated upon heat stress. Heterologous expression of VvGOLS1 in Escherichia coli showed that it encodes a functional galactinol synthase. Transient expression assays showed that the heat stress factor VvHsfA2 transactivates the promoter of VvGOLS1 in a heat stress-dependent manner. Taken together, our results highlight the intrinsic capacity of grape berries to perceive heat stress and to initiate adaptive responses, suggesting that galactinol may play a signaling role in these responses

Published
2017

37. Phosphoproteins Involved in the Signal Transduction of Cryptogein, an Elicitor of Defense Reactions in Tobacco

Author

Lecourieux, Fatma, Lecourieux-Ouaked, Fatma, Pugin, Alain, Lebrun-Garcia, Angela, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB), and Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Physiology, Phosphatase, Biology, 01 natural sciences, Fungal Proteins, 03 medical and health sciences, chemistry.chemical_compound, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Tobacco, Phosphoprotein Phosphatases, medicine, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Staurosporine, Protein phosphorylation, Enzyme Inhibitors, Phosphorylation, Protein Kinase Inhibitors, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Fungal protein, Ion Transport, Algal Proteins, General Medicine, Phosphoproteins, Elicitor, Plants, Toxic, chemistry, Biochemistry, Calcium, Signal transduction, Agronomy and Crop Science, Signal Transduction, 010606 plant biology & botany, Calyculin, medicine.drug
Abstract

We previously reported that the signal transduction of cryptogein, an elicitor of defense reactions in Nicotiana tabacum cells, involves upstream protein phosphorylation. In the present study, induction of these early physiological events was further investigated with inhibitors of protein phosphatase (PP), okadaïc acid, and calyculin A. Calyculin A mimicked the effects of cryptogein, inducing an influx of calcium, an extracellular alkalinization, and the production of active oxygen species (AOS), suggesting that during cryptogein signal transduction the balance between specific protein kinase (PK) and PP activities was modified. To identify the phosphorylated proteins that could be involved early in the elicitor signaling pathway, we analyzed by 2-D electrophoresis the in vivo phosphorylation status of proteins after cryptogein, staurosporine, and calyculin A treatments of tobacco cells (5 min). Of about 100 phospholabeled polypeptides, 19 showed increased 32P incorporation after 5 min of cryptogein treatment. Phosphorylation of 12 of the 19 polypeptides depended upon calcium influx. Staurosporine inhibited the phosphorylations induced by cryptogein whereas calyculin A activated the phosphorylation of 18 of these polypeptides. This study highlighted the role of PKs and/or constitutive active PPs whose activation and inhibition, respectively, resulted in an increased phosphorylation of proteins that may be involved in cryptogein signal transduction. Identification of the phosphoproteins is in progress and will increase our knowledge of signal transduction pathways implicated in plant defense responses.

Published
2000
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38. The basic leucine zipper transcription factor abscisic acid responseelement-binding factor 2 is an important transcriptional regulator ofabscisic acid-dependent grape berry ripening processes

Author

Nicolas, Philippe, Lecourieux, David, Kappel, Christian, Cluzet, Stephanie, Cramer, Grant, Delrot, Serge, and Lecourieux, Fatma

Subjects
fungi, food and beverages, Institut für Biochemie und Biologie
Abstract

In grape (Vitis vinifera), abscisic acid (ABA) accumulates during fruit ripening and is thought to play a pivotal role in this process, but the molecular basis of this control is poorly understood. This work characterizes ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 (VvABF2), a grape basic leucine zipper transcription factor belonging to a phylogenetic subgroup previously shown to be involved in ABA and abiotic stress signaling in other plant species. VvABF2 transcripts mainly accumulated in the berry, from the onset of ripening to the harvesting stage, and were up-regulated by ABA. Microarray analysis of transgenic grape cells overexpressing VvABF2 showed that this transcription factor up-regulates and/or modifies existing networks related to ABA responses. In addition, grape cells overexpressing VvABF2 exhibited enhanced responses to ABA treatment compared with control cells. Among the VvABF2-mediated responses highlighted in this study, the synthesis of phenolic compounds and cell wall softening were the most strongly affected. VvABF2 overexpression strongly increased the accumulation of stilbenes that play a role in plant defense and human health (resveratrol and piceid). In addition, the firmness of fruits from tomato (Solanum lycopersicum) plants overexpressing VvABF2 was strongly reduced. These data indicate that VvABF2 is an important transcriptional regulator of ABA-dependent grape berry ripening.

Published
2014

39. The Basic Leucine Zipper Transcription Factor ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 Is an Important Transcriptional Regulator of Abscisic Acid-Dependent Grape Berry Ripening Processes1[W][OPEN]

Author

Nicolas, Philippe, Lecourieux, David, Kappel, Christian, Cluzet, Stéphanie, Cramer, Grant, Delrot, Serge, and Lecourieux, Fatma

Subjects
fungi, Molecular Sequence Data, food and beverages, Plants, Genetically Modified, Article, Basic-Leucine Zipper Transcription Factors, Solanum lycopersicum, Gene Expression Regulation, Plant, Fruit, Stilbenes, Vitis, Amino Acid Sequence, Phylogeny, Abscisic Acid, Oligonucleotide Array Sequence Analysis, Plant Proteins
Abstract

In grape (Vitis vinifera), abscisic acid (ABA) accumulates during fruit ripening and is thought to play a pivotal role in this process, but the molecular basis of this control is poorly understood. This work characterizes ABSCISIC ACID RESPONSE ELEMENT-BINDING FACTOR2 (VvABF2), a grape basic leucine zipper transcription factor belonging to a phylogenetic subgroup previously shown to be involved in ABA and abiotic stress signaling in other plant species. VvABF2 transcripts mainly accumulated in the berry, from the onset of ripening to the harvesting stage, and were up-regulated by ABA. Microarray analysis of transgenic grape cells overexpressing VvABF2 showed that this transcription factor up-regulates and/or modifies existing networks related to ABA responses. In addition, grape cells overexpressing VvABF2 exhibited enhanced responses to ABA treatment compared with control cells. Among the VvABF2-mediated responses highlighted in this study, the synthesis of phenolic compounds and cell wall softening were the most strongly affected. VvABF2 overexpression strongly increased the accumulation of stilbenes that play a role in plant defense and human health (resveratrol and piceid). In addition, the firmness of fruits from tomato (Solanum lycopersicum) plants overexpressing VvABF2 was strongly reduced. These data indicate that VvABF2 is an important transcriptional regulator of ABA-dependent grape berry ripening.

Published
2013

40. Direct impact of high temperature on the grapevine berry proteome

Author

LECOURIEUX, Fatma, KAPPEL, Christian, PIERI, Philippe, CHARON, Justine, GOMES, Eric, DELROT, Serge, LECOURIEUX, David, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

41. Direct impact of high temperature on Cabernet Sauvignon berries development and metabolism

Author

Lecourieux, David, Pillet, Jérémy, Lecourieux, Fatma, Kappel, Christian, Pieri, Philippe, Charon, Justine, Hilbert, Ghislaine, Renaud, Christel, Gomes, Eric, Delrot, Serge, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2013

42. Grape berry development and composition: recent data and issues

Author

Berdeja, Mariam, Dai, Zhanwu, Destrac, Agnes, Gomes, Eric, Hilbert, Ghislaine, Lecourieux, David, Lecourieux, Fatma, van Leeuwen, Cornelis, Martinez Luscher, Johann, Ollat, Nathalie, Parker, Amber, Pillet, Jérémy, Pieri, Philippe, Vivin, Philippe, Delrot, Serge, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2013

43. VvCEB1, a grape bHLH transcription factor affects cell size through the auxin balance

Author

Lecourieux, David, Gomes, Eric, Lecourieux, Fatma, Delrot, Serge, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2012

44. Source/Sink Relationships and Molecular Biology of Sugar Accumulation in Grape Berries

Author

Davies, C., Boss, P.K., Geros, Hernani, Lecourieux, Fatma, Delrot, Serge, Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), and Hernâni Gerós, M. Manuela Chaves and Serge Delrot

Subjects
0106 biological sciences, 2. Zero hunger, 0303 health sciences, [SDV]Life Sciences [q-bio], fungi, food and beverages, 15. Life on land, 01 natural sciences, Assimilates, Disaccharide transporter, fructose, glucose, invertase, monosaccharide transporter, phloem transport, phloem unloading, sucrose, sugars, veraison, 03 medical and health sciences, [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, 030304 developmental biology, 010606 plant biology & botany
Abstract

International audience; Phloem transport of assimilates provides the materials needed for the growth and development of reproductive structures, storage and developing organs, and has long been recognised as a major determinant in crop yield. Thus, the understanding of the mechanisms and regulations of sugar transport into sink tissues has an important basic and applied relevance. The Grapevine is a good example of a crop where sugar accumulation in the fruit has an important economic role. Massive sugar transport and compartmentation into the grape berry mesocarp cells (up to 1 M glucose and fructose) start at veraison and continues until the harvest. Sucrose transported in the phloem is cleaved into hexoses by invertases and stored in the vacuole. The Sugar content determines the sweetness of table grapes, wine alcohol content, and regulates gene expression, including, for example, several genes involved in the synthesis of secondary compounds which contribute to grape and wine quality. Many viticultural practices affect source/sink relationships, thus altering sugar concentration in the berry. For instance, the rootstock used, which is a potential sink, has a strong impact on source activity, by affecting the morphology and activity of the aerial part of the plant. Molecular approaches have also provided major advances in grapevine research. Monosaccharide and disaccharide transporter genes have been recently identified and their products studied in heterologous systems. The sequencing of the grapevine genome and the development of grape microarrays have made a valuable contribution to the study of the biochemistry of grape berry development and ripening, for example, low affinity glucose uniporters identified in the genome may also be involved in the sugar uptake. In the present chapter, the routes of sugar import and storage in the grape cells are updated and discussed and a model with the main transport steps and biochemical pathways is proposed.

Published
2012
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45. Identification and functional characterization of potential targets for genetic improvement of grape berry quality

Author

Hichri, Imene, Kappel, Christian, Mahjoub, Aly, Pillet, Jérémy, Barrieu, Francois, Destrac, Agnes, Guillaumie, Sabine, Hilbert, Ghislaine, Lecourieux, Fatma, Lecourieux, David, Lauvergeat, Virginie, Léon, Céline, Pieri, Philippe, Trossat, Claudine, Gomes, Eric, Delrot, Serge, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

46. Identification and functional characterization of a bHLH transcription factor involved in the ripening of fleshy fruits

Author

Nicolas, Philippe, LECOURIEUX, Fatma, Lecourieux, David, GOMES, Eric, Delrot, Serge, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

47. Trancriptomic analysis to decipher the molecular basis of grapevine primary metabolism regulations

Author

Camps, Céline, Kappel, Christian, LECOURIEUX, Fatma, Lecourieux, David, Lecomte, Pascal, Coutos-Thévenot, P., GOMES, Eric, Delrot, Serge, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) (UMRSV), Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut des Sciences de la Vigne et du Vin (ISVV), Equipe Physiologie Moléculaire du Transport de Sucres (PhyMoTS), Laboratoire de catalyse en chimie organique (LACCO), and Université de Poitiers-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

48. Transcriptomics and molecular biology of grapevine ripening

Author

Kappel, Christian, Pillet, Jérémy, Guillaumie, Sabine, Hichri, Imene, Mahjoub, Aly, Lecourieux, David, LECOURIEUX, Fatma, Pieri, Philippe, Barrieu, Francois, Lauvergeat, Virginie, GOMES, Eric, Delrot, Serge, ProdInra, Migration, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

49. Molecular and systems biology of grapevine ripening

Author

Kappel, Christian, Pillet, Jérémy, Lecourieux, David, LECOURIEUX, Fatma, Hilbert, Ghislaine, Moing, Annick, GOMES, Eric, Ollat, Nathalie, Delrot, Serge, ProdInra, Migration, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Station de physiologie végétale, and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2010

50. Identification and functional characterization of a bHLH transcription factor involved in fleshy fruit ripening

Author

Nicolas, Philippe, Lecourieux, Fatma, Lecourieux, David, Delrot, Serge, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Universidade do Minho, and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)

Subjects
vitis vinifera, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, grappe de raisin, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, viticulture, génotype, transcriptome, composition des baies, ComputingMilieux_MISCELLANEOUS, qualité du fruit
Abstract

National audience

Published
2009

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