Additional file 2 of Knockout of VvCCD8 gene in grapevine affects shoot branching

Additional file 2: Figure S2. Sequencing results of the two putative off-target sites in VvCCD8 knockout lines. The two off-target sites predicted within exons of other genes were selected for off-target analysis. Two VvCCD8 knockout lines, Plant #1 and Plant #6 were used in the experiment. The amplified fragments containing the off-target sites were amplified and cloned into pLB-Simple vector. At least 6 clones for each site were used for Sanger sequencing.