1. Hypoxia-Activated, Small-Molecule-Induced Gene Expression
- Author
-
Laure C. Bouchez, Jaideep Saha, Ester M. Hammond, Stuart J. Conway, and Sarah L. Collins
- Subjects
Isopropyl Thiogalactoside ,0301 basic medicine ,Nitrofurans ,Green Fluorescent Proteins ,Gene Expression ,Biochemistry ,Green fluorescent protein ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,Oxazines ,Gene expression ,Escherichia coli ,medicine ,Humans ,Prodrugs ,Inducer ,Anaerobiosis ,Chemistry ,Biofilm ,General Medicine ,Prodrug ,Hypoxia (medical) ,Small molecule ,3. Good health ,Cell biology ,030104 developmental biology ,Cell culture ,030220 oncology & carcinogenesis ,Molecular Medicine ,medicine.symptom - Abstract
Hypoxia, conditions of reduced oxygen, occur in a wide variety of biological contexts, including solid tumours and bacterial biofilms, which are relevant to human health. Consequently, the development of chemical tools to study hypoxia is vital. Here we report a hypoxia-activated small molecule-mediated gene expression system using a bioreductive prodrug of the inducer isopropyl 1-thio-β-D-galactopyranoside (IPTG). As a proof-of-concept we have placed the production of a green fluorescent protein under the control of hypoxia. Our system has the potential to be extended to regulate the production any given protein of choice.
- Published
- 2018