11 results on '"Latorre-Ossa, Heldmuth"'
Search Results
2. Supersonic Shear Wave Elastography of Response to Anti-cancer Therapy in a Xenograft Tumor Model
- Author
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Chamming's, Foucauld, Le-Frère-Belda, Marie-Aude, Latorre-Ossa, Heldmuth, Fitoussi, Victor, Redheuil, Alban, Assayag, Franck, Pidial, Laetitia, Gennisson, Jean-Luc, Tanter, Mickael, Cuénod, Charles-André, and Fournier, Laure S.
- Published
- 2016
- Full Text
- View/download PDF
3. Mechanical induction of the tumorigenic β-catenin pathway by tumour growth pressure
- Author
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Fernandez-Sanchez, Maria Elena, Barbier, Sandrine, Whitehead, Joanne, Bealle, Gaelle, Michel, Aude, Latorre-Ossa, Heldmuth, Rey, Colette, Fouassier, Laura, Claperon, Audrey, Brulle, Laura, Girard, Elodie, Servant, Nicolas, Rio-Frio, Thomas, Marie, Helene, Lesieur, Sylviane, Housset, Chantal, Gennisson, Jean-Luc, Tanter, Mickael, Menager, Christine, Fre, Silvia, Robine, Sylvie, and Farge, Emmanuel
- Subjects
Epithelium -- Physiological aspects -- Observations ,Tumors -- Growth ,Carcinogenesis -- Research ,Company growth ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
The tumour microenvironment may contribute to tumorigenesis owing to mechanical forces such as fibrotic stiffness or mechanical pressure caused by the expansion of hyper-proliferative cells (1,2). Here we explore the contribution of the mechanical pressure exerted by tumour growth onto non-tumorous adjacent epithelium. In the early stage of mouse colon tumour development in the [Notch.sup.+][Apc.sup.+/1638N] mouse model, we observed mechanistic pressure stress in the non-tumorous epithelial cells caused by hyper-proliferative adjacent crypts overexpressing active Notch, which is associated with increased Ret and β-catenin signalling. We thus developed a method that allows the delivery of a defined mechanical pressure invivo, by subcutaneously inserting a magnet close to the mouse colon. The implanted magnet generated a magnetic force on ultra-magnetic liposomes, stabilized in the mesenchymal cells of the connective tissue surrounding colonic crypts after intravenous injection. The magnetically induced pressure quantitatively mimicked the endogenous early tumour growth stress in the order of 1,200 Pa, without affecting tissue stiffness, as monitored by ultrasound strain imaging and shear wave elastography. The exertion of pressure mimicking that of tumour growth led to rapid Ret activation and downstream phosphorylation of β-catenin on Tyr654, imparing its interaction with the E-cadherin in adherens junctions, and which was followed by β-catenin nuclear translocation after 15 days. As a consequence, increased expression of β-catenin-target genes was observed at 1 month, together with crypt enlargement accompanying the formation of early tumorous aberrant crypt foci. Mechanical activation of the tumorigenic β-catenin pathway suggests unexplored modes of tumour propagation based on mechanical signalling pathways in healthy epithelial cells surrounding the tumour, which may contribute to tumour heterogeneity., To test the tumorous impact of early tumour growth pressure on non-tumorous epithelial tissues in vivo, apart from the mechanical stiffness characteristic of the microenvironment of late tumours (1-6) (see [...]
- Published
- 2015
4. Suivi des propriétés élastiques pendant la croissance et la thérapie du cancer
- Author
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Latorre Ossa, Heldmuth, Laboratoire Ondes et Acoustique (UMR 7587) (LOA), Université Paris Diderot - Paris 7 (UPD7)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Institut Langevin - Ondes et Images (UMR7587) (IL), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Diderot - Paris VII, Mickaël TanterJean-Luc Gennisson(mickael.tanter@espci.fr), Sorbonne Université (SU)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
shear wave elastography ,SSI technique ,élastographie ,elastography ,tumeur ,tumour ,technique SSI ,ultrasons ,ultrasound elasticity ,élasticité ,[PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph] ,cancer ,therapeutic treatment - Abstract
Throughout this PhD thesis, a technique developed at the Institut Langevin called the Supersonic Shear Imaging (SSI) technique, capable of quantifying tissue shear elasticity in real time, was employed for the characterization of biological tissues. Pre-clinical and clinical studies were performed to evaluate the potential of the technique in the monitoring of elastic changes during cancer development and therapeutic treatment. A clinical study was performed on 23 patients presenting breast lesions who underwent chemotherapy treatment at the Institut Curie. The goal of the study was to use for the very first time, 3D-Ultrasound (3D-US) and 3D-Shear Wave Elastography (3D-SWE) during the chemotherapy treatment to estimate tumour volume and tumour global elasticity, respectively. The 3D-US estimated tumour volumes were compared with Magnetic Resonance Imaging (MRI) volume estimations and showed good correlation. The results of the study demonstrated that 3D-SWE allowed the monitoring the tumour reaction to the chemotherapy by offering accurate tumour elasticity measurements in three-dimensions throughout the study. Tumour elasticity was found to be well correlated with the 3D-US and MRI tumour volume estimations. The SSI technique was employed for the monitoring of cancerous tissues, in the frame of two pre-clinical studies performed on nude mice. One of the studies, carried out in collaboration with L'hôpital Européen Georges-Pompidou (HEGP), aimed to understand the pathology underlying stiffness. The SSI technique and US imaging were used to monitor the growth and the reaction to chemotherapy of human carcinomas implanted on mice. The results showed that for this particular tumour model, tumour volume and tumour elasticity were very well correlated. Performed histo-pathological analyses revealed that tumour elasticity also was very well correlated with tumour necrosis and fibrosis. The second pre-clinical study, performed in collaboration with the faculty of Pharmacy of L'Universite Paris V Descartes, intended to evaluate the feasibility of using tissue elasticity to assess the effectiveness of an anti-vascular treatment on a particular type of human colon carcinoma implanted in mice. The results did not show a clear elasticity tendency associated to the treatment. In order to study new additional mechanical parameters (apart from tissue elasticity) which allow a better tissue characterization, a method combining quasi-static axial compressions and the SSI technique was developed to retrieve tissue shear nonlinearity. The first known 2D nonlinearity maps were calculated on tissue mimicking phantoms and on ex-vivo beef liver samples with good accuracy. The fact that the developed technique could be easily implemented on standard imaging probes driven by ultrafast US scanners, make it transferable to clinics.; Au cours de cette thèse une technique d'imagerie ultrasonore nommée "Supersonic Shear Imaging (SSI)", capable de quantifier en temps réel l'élasticité de cisaillement des tissus, a été utilisée pour la caractérisation des tissus biologiques. Des études cliniques et précliniques ont été réalisées pour évaluer le potentiel de la technique pour suivre les changements de propriétés élastiques dus au développement de cancer ou encore aux traitements thérapeutiques. Une étude clinique a été réalisée sur 23 patientes présentant des lésions du sein ayant subi un traitement de chimiothérapie à l'Institut Curie. Le but de cette étude était d'utiliser pour la première fois l'élastographie 3D pour estimer le volume et l'élasticité globale de tumeurs en cours de traitement par chimiothérapie. Les volumes tumoraux estimés sur les images échographiques ont été comparés aux estimations d'imagerie par résonnance magnétique (IRM) et ont montré une bonne corrélation. Les résultats de l'étude ont montré que la 3D-SWE permet de suivre la réaction tumorale à la chimiothérapie en offrant des mesures précises d'élasticité tumorale en trois dimensions tout au long de l'étude. La technique SSI a été utilisée pour la surveillance des tissus cancéreux dans le cadre de deux études précliniques, réalisées chez des souris nues. L'une des études, menée en collaboration avec l'Hôpital Européen Georges-Pompidou (HEGP), vise à comprendre la rigidité pathologie sous-jacente. L'imagerie a été utilisée pour surveiller la croissance et la réaction à la chimiothérapie de carcinomes humains implantés sur des souris. Les résultats ont montré que pour ce modèle particulier de tumeur, le volume tumoral et l'élasticité de la tumeur étaient très bien corrélées. Les analyses histo-pathologiques ont révélé que l'élasticité de la tumeur est également très bien corrélée avec la nécrose tumorale et la fibrose. La deuxième étude préclinique, réalisée en collaboration avec la Faculté de Pharmacie de l'Université Paris Descartes V, a permis d'évaluer la faisabilité d'utiliser l'imagerie d'élasticité pour suivre un traitement anti-vasculaire sur un type particulier de carcinome du côlon humain implanté chez des souris. Les résultats ne montrent pas de tendance claire de variation de l'élasticité associée au traitement. Afin d'étudier de nouveaux paramètres mécaniques supplémentaires qui permettent une meilleure caractérisation des tissus, une méthode combinant des compressions quasi-statiques axiales et la technique SSI a été mise au point pour mesurer la non-linéarité de cisaillement des tissus. Les premières cartes connues de non-linéarité 2D ont été calculées sur des fantômes imitant les tissus et des échantillons ex vivo de foie de boeuf avec une bonne précision.
- Published
- 2012
5. In vivo monitoring of elastic changes during cancer development and therapeutic treatment
- Author
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Latorre Ossa, Heldmuth, Laboratoire Ondes et Acoustique (UMR 7587) (LOA), Université Paris Diderot - Paris 7 (UPD7)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Institut Langevin - Ondes et Images (UMR7587) (IL), Sorbonne Université (SU)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Diderot - Paris VII, and Mickaël TanterJean-Luc Gennisson(mickael.tanter@espci.fr)
- Subjects
shear wave elastography ,SSI technique ,élastographie ,elastography ,tumeur ,tumour ,technique SSI ,ultrasons ,ultrasound elasticity ,élasticité ,[PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph] ,cancer ,therapeutic treatment - Abstract
Throughout this PhD thesis, a technique developed at the Institut Langevin called the Supersonic Shear Imaging (SSI) technique, capable of quantifying tissue shear elasticity in real time, was employed for the characterization of biological tissues. Pre-clinical and clinical studies were performed to evaluate the potential of the technique in the monitoring of elastic changes during cancer development and therapeutic treatment. A clinical study was performed on 23 patients presenting breast lesions who underwent chemotherapy treatment at the Institut Curie. The goal of the study was to use for the very first time, 3D-Ultrasound (3D-US) and 3D-Shear Wave Elastography (3D-SWE) during the chemotherapy treatment to estimate tumour volume and tumour global elasticity, respectively. The 3D-US estimated tumour volumes were compared with Magnetic Resonance Imaging (MRI) volume estimations and showed good correlation. The results of the study demonstrated that 3D-SWE allowed the monitoring the tumour reaction to the chemotherapy by offering accurate tumour elasticity measurements in three-dimensions throughout the study. Tumour elasticity was found to be well correlated with the 3D-US and MRI tumour volume estimations. The SSI technique was employed for the monitoring of cancerous tissues, in the frame of two pre-clinical studies performed on nude mice. One of the studies, carried out in collaboration with L'hôpital Européen Georges-Pompidou (HEGP), aimed to understand the pathology underlying stiffness. The SSI technique and US imaging were used to monitor the growth and the reaction to chemotherapy of human carcinomas implanted on mice. The results showed that for this particular tumour model, tumour volume and tumour elasticity were very well correlated. Performed histo-pathological analyses revealed that tumour elasticity also was very well correlated with tumour necrosis and fibrosis. The second pre-clinical study, performed in collaboration with the faculty of Pharmacy of L'Universite Paris V Descartes, intended to evaluate the feasibility of using tissue elasticity to assess the effectiveness of an anti-vascular treatment on a particular type of human colon carcinoma implanted in mice. The results did not show a clear elasticity tendency associated to the treatment. In order to study new additional mechanical parameters (apart from tissue elasticity) which allow a better tissue characterization, a method combining quasi-static axial compressions and the SSI technique was developed to retrieve tissue shear nonlinearity. The first known 2D nonlinearity maps were calculated on tissue mimicking phantoms and on ex-vivo beef liver samples with good accuracy. The fact that the developed technique could be easily implemented on standard imaging probes driven by ultrafast US scanners, make it transferable to clinics.; Au cours de cette thèse une technique d'imagerie ultrasonore nommée "Supersonic Shear Imaging (SSI)", capable de quantifier en temps réel l'élasticité de cisaillement des tissus, a été utilisée pour la caractérisation des tissus biologiques. Des études cliniques et précliniques ont été réalisées pour évaluer le potentiel de la technique pour suivre les changements de propriétés élastiques dus au développement de cancer ou encore aux traitements thérapeutiques. Une étude clinique a été réalisée sur 23 patientes présentant des lésions du sein ayant subi un traitement de chimiothérapie à l'Institut Curie. Le but de cette étude était d'utiliser pour la première fois l'élastographie 3D pour estimer le volume et l'élasticité globale de tumeurs en cours de traitement par chimiothérapie. Les volumes tumoraux estimés sur les images échographiques ont été comparés aux estimations d'imagerie par résonnance magnétique (IRM) et ont montré une bonne corrélation. Les résultats de l'étude ont montré que la 3D-SWE permet de suivre la réaction tumorale à la chimiothérapie en offrant des mesures précises d'élasticité tumorale en trois dimensions tout au long de l'étude. La technique SSI a été utilisée pour la surveillance des tissus cancéreux dans le cadre de deux études précliniques, réalisées chez des souris nues. L'une des études, menée en collaboration avec l'Hôpital Européen Georges-Pompidou (HEGP), vise à comprendre la rigidité pathologie sous-jacente. L'imagerie a été utilisée pour surveiller la croissance et la réaction à la chimiothérapie de carcinomes humains implantés sur des souris. Les résultats ont montré que pour ce modèle particulier de tumeur, le volume tumoral et l'élasticité de la tumeur étaient très bien corrélées. Les analyses histo-pathologiques ont révélé que l'élasticité de la tumeur est également très bien corrélée avec la nécrose tumorale et la fibrose. La deuxième étude préclinique, réalisée en collaboration avec la Faculté de Pharmacie de l'Université Paris Descartes V, a permis d'évaluer la faisabilité d'utiliser l'imagerie d'élasticité pour suivre un traitement anti-vasculaire sur un type particulier de carcinome du côlon humain implanté chez des souris. Les résultats ne montrent pas de tendance claire de variation de l'élasticité associée au traitement. Afin d'étudier de nouveaux paramètres mécaniques supplémentaires qui permettent une meilleure caractérisation des tissus, une méthode combinant des compressions quasi-statiques axiales et la technique SSI a été mise au point pour mesurer la non-linéarité de cisaillement des tissus. Les premières cartes connues de non-linéarité 2D ont été calculées sur des fantômes imitant les tissus et des échantillons ex vivo de foie de boeuf avec une bonne précision.
- Published
- 2012
6. Feasibility of Imaging and Treatment Monitoring of Breast Lesions with Three-Dimensional Shear Wave Elastography
- Author
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Athanasiou, Alexandra, additional, Latorre-Ossa, Heldmuth, additional, Criton, Aline, additional, Tardivon, Anne, additional, Gennisson, Jean-Luc, additional, and Tanter, Mickael, additional
- Published
- 2015
- Full Text
- View/download PDF
7. A protocol for in vivo magnetisation of mice colon and application of physiological mechanical deformations to study mechanotransduction in tumour growth pressure
- Author
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Maria Elena, Fernandez Sanchez, primary, Fernandez-Sanchez, Maria Elena, additional, Barbier, Sandrine, additional, Latorre-Ossa, Heldmuth, additional, Marie, Hélène, additional, Rey, Colette, additional, Fouassier, Laura, additional, Claperon, Audrey, additional, Housset, Chantal, additional, Lesieur, Sylviane, additional, Gennisson, Jean-Luc, additional, Tanter, Mickaël, additional, and Farge, Emmanuel, additional
- Published
- 2014
- Full Text
- View/download PDF
8. Feasibility of Imaging and Treatment Monitoring of Breast Lesions with Three-Dimensional Shear Wave Elastography.
- Author
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Athanasiou, Alexandra, Latorre-Ossa, Heldmuth, Criton, Aline, Tardivon, Anne, Gennisson, Jean-Luc, and Tanter, Mickael
- Published
- 2017
- Full Text
- View/download PDF
9. Evaluation of Nonradiative Clinical Imaging Techniques for the Longitudinal Assessment of Tumour Growth in Murine CT26 Colon Carcinoma
- Author
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Seguin, Johanne, primary, Doan, Bich-Thuy, additional, Latorre Ossa, Heldmuth, additional, Jugé, Lauriane, additional, Gennisson, Jean-Luc, additional, Tanter, Mickaël, additional, Scherman, Daniel, additional, Chabot, Guy G., additional, and Mignet, Nathalie, additional
- Published
- 2013
- Full Text
- View/download PDF
10. Quantitative imaging of nonlinear shear modulus by combining static elastography and shear wave elastography.
- Author
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Latorre-Ossa, Heldmuth, Gennisson, Jean-Luc, De Brosses, Emilie, and Tanter, Mickael
- Subjects
- *
QUANTITATIVE research , *ULTRASONIC imaging , *NONLINEAR theories , *STRAINS & stresses (Mechanics) , *SHEAR waves , *TISSUE engineering , *IMAGE compression , *IMAGING phantoms , *ELASTICITY - Abstract
The study of new tissue mechanical properties such as shear nonlinearity could lead to better tissue characterization and clinical diagnosis. This work proposes a method combining static elastography and shear wave elastography to derive the nonlinear shear modulus by applying the acoustoelasticity theory in quasi-incompressible soft solids. Results demonstrate that by applying a moderate static stress at the surface of the investigated medium, and by following the quantitative evolution of its shear modulus, it is possible to accurately and quantitatively recover the local Landau (A) coefficient characterizing the shear nonlinearity of soft tissues. [ABSTRACT FROM PUBLISHER]
- Published
- 2012
- Full Text
- View/download PDF
11. Feasibility of Imaging and Treatment Monitoring of Breast Lesions with Three-Dimensional Shear Wave Elastography.
- Author
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Athanasiou A, Latorre-Ossa H, Criton A, Tardivon A, Gennisson JL, and Tanter M
- Subjects
- Adult, Aged, Breast Neoplasms drug therapy, Chemotherapy, Adjuvant, Feasibility Studies, Humans, Magnetic Resonance Imaging methods, Mammography methods, Middle Aged, Neoadjuvant Therapy, Prospective Studies, Treatment Outcome, Tumor Burden physiology, Breast Neoplasms diagnostic imaging, Elasticity Imaging Techniques methods, Imaging, Three-Dimensional methods, Ultrasonography, Mammary methods
- Abstract
Purpose Firstly to evaluate the feasibility and diagnostic performance of three-dimensional (3 D) shear wave elastography (SWE) volume measurements in patients with breast lesions compared to breast dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) lesion volumes and 3D-US B-mode volumes. Secondly to assess the treatment monitoring performance of 3D-SWE in patients under neoadjuvant chemotherapy for breast cancer by comparing it to 3D-US lesion volume. Materials and Methods This prospective study was approved by the institutional review board. Informed consent was provided. 33 patients with 33 lesions were included. The feasibility of 3D-SWE was evaluated in 23 patients. In the 10 remaining patients receiving neoadjuvant chemotherapy, 3D-SWE was evaluated before and during treatment. Tumor volume and qualitative and quantitative elasticity analysis measurements were performed and compared to the tumor volume as estimated by 3D-US and DCE-MRI. Statistical analysis was performed using the Pearson correlation coefficient. Results 3D-SWE was feasible in patients with breast lesions. Tumor volume calculated with 3D-US and 3D-SWE showed very good and moderate concordances with DCE-MRI volume, respectively (Pearson correlation coefficients equal to ρ = r = 0.88, p < 0.00 002 and ρ = r = 0.5, p = 0.32, respectively). Modification of tumor elasticity and heterogeneity was correlated with response to treatment. In good responders, elasticity and elasticity heterogeneity diminished. Conclusion Tumor 3D-US volume measurements showed very good concordance with DCE-MRI volume. 3D-SWE can provide valuable information: reduction of tissue stiffness during treatment could be a potential indicator of response. These preliminary results should be confirmed on a larger number of patients., (© Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2017
- Full Text
- View/download PDF
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