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Botanical surveys carried out during the last 13 years throughout central and northern Tunisia (North Africa) allow to find first national and continental N-African records of non-native taxa belonging to Bignoniaceae. The genera Catalpa, Podranea, and Tecomaria are new for N-Africa (Catalpa is new for the whole Africa). Tecomaria capensis is here considered as a naturalized alien species, whereas Campsis radicans, Catalpa bignonioides, Jacaranda mimosifolia, Podranea brycei, P. ricasoliana, and Tecoma stans are casuals. Distribution in Tunisia, phenology, and original photographs are provided for each species. A diagnostic key for Tunisian Bignoniaceae is also given. Moreover, the name Tecoma ricasoliana (basionym of Podranea ricasoliana) is lectotypified on a specimen preserved at K (isolectotypes at FI), whereas the holotype indication of the name Bignonia capensis (basionym of Tecomaria capensis), made in Flora of Tropical East Africa, is here corrected according to the Art. 9.10 of Shenzhen Code (lectotype at UPS; isolectotype, here reported for the first time, at LD). [ABSTRACT FROM AUTHOR]

Lamiales, comprising over 23,755 species across 24 families, stands as a highly diverse and prolific plant group, playing a significant role in the cultivation of horticultural, ornamental, and medicinal plant varieties. Whole-genome duplication (WGD) and its subsequent post-polyploid diploidization (PPD) process represent the most drastic type of karyotype evolution, injecting significant potential for promoting the diversity of this lineage. However, polyploidization histories, as well as genome and subgenome fractionation following WGD events in Lamiales species, are still not well investigated. In this study, we constructed a chromosome-level genome assembly of Lindenbergia philippensis (Orobanchaceae) and conducted comparative genomic analyses with 14 other Lamiales species. L. philippensis is positioned closest to the parasitic lineage within Orobanchaceae and has a conserved karyotype. Through a combination of Ks analysis and syntenic depth analysis, we reconstructed and validated polyploidization histories of Lamiales species. Our results indicated that Primulina huaijiensis underwent three rounds of diploidization events following the γ-WGT event, rather than two rounds as reported. Besides, we reconfirmed that most Lamiales species shared a common diploidization event (L-WGD). Subsequently, we constructed the Lamiales Ancestral Karyotype (LAK), comprising 11 proto-chromosomes, and elucidated its evolutionary trajectory, highlighting the highly flexible reshuffling of the Lamiales paleogenome. We identified biased fractionation of subgenomes following the L-WGD event across eight species, and highlighted the positive impacts of non-WGD genes on gene family expansion. This study provides novel genomic resources and insights into polyploidy and karyotype remodeling of Lamiales species, essential for advancing our understanding of species diversification and genome evolution. [ABSTRACT FROM AUTHOR]

Summary: Ruellia taboleirana (Acanthaceae) was originally described from specimens restricted to the tabuleiros litorâneos on the Northeast Brazilian coast. Here, we expand its geographic distribution to coastal areas with Amazonian influence and make important contributions on its morphology and ecology. We also present the first photos in vivo that evidence its morphological variation, along with scanning electron micrographs of pollen grains, an updated conservation status assessment, and a detailed line drawing of this species for the first time. Finally, we provide an identification key for all species of Ruellia that occur in the state of Maranhão. [ABSTRACT FROM AUTHOR]

Summary: Salvia sect. Angulatae and S. sect. Nobiles are among the sections of Salvia L. (Lamiaceae) with most of their Brazilian species represented in the Atlantic Forest and the Cerrado domains, which are important regions of species richness for the genus. After extensive evaluation of the literature, populations in situ and specimens in herbaria, including nomenclatural types, we identified a requirement for taxonomic and nomenclatural changes in S. arenaria and S. sellowiana, and for updated identification keys and taxonomic notes for the other members of these sections, particularly S. rivularis in S. sect. Nobiles. Two names are synonymised, Salvia articulata = S. sellowiana and S. itatiaiensis = S. arenaria and notes on the nomenclature and morphology of these species are provided. A first-step lectotypification (S. arenaria var. sellowii) and a second-step lectotypification (S. itatiaiensis) are also presented, as well as detailed descriptions for S. arenaria and S. sellowiana and preliminary conservation status assessments for both. [ABSTRACT FROM AUTHOR]

Bacopa sessiliflora (Benth.) Edwall (Plantaginaceae) is an aquatic herb that inhabits the banks of watercourses in the Neotropics. In this study, we confirmed the occurrence of the species in the Cerrado following a field collection on the banks of the Preto River, in eastern Maranhão state, Brazil, and the study of herbarium materials. A morphological description, illustrations, phenological data, and a distribution map of B. sessiliflora are provided. The new data highlight the importance of permanent protected areas, especially riverbanks, as crucial habitats for increasing our understanding of plant biodiversity in Maranhão.

AbstractStrobilanthes dalzielii of Acanthaceae is an herb species with potentially extensive applications for its pharmaceutical and ornamental values. Due to taxonomic complications and limited genetic information, the structural characteristics, and phylogenetic relationships of the S. dalzielii chloroplast genome were assembled and characterized here for the first time. The complete chloroplast genome of S. dalzielii was 144,580 bp in length. The genome is quadripartite in structure and consists of a large single-copy region (92,137 bp) and a small single-copy region (17,669 bp), which are separated by a pair of inverted repeats (each 17,387 bp). A total of 125 genes were annotated, including 80 protein-coding, 37 transfer RNA, and eight ribosomal RNA genes. The overall GC content was 36.4%. Phylogenetic analysis based on the complete chloroplast genome sequence of 21 taxa within the tribe Ruellieae of Acanthaceae using the maximum likelihood and Bayesian inference methods revealed that Strobilanthes diverged after Ruellia; S. dalzielii is closely related to S. tonkinensis. The genomic data obtained from this study will serve as valuable information to the species delimitation and genetic classification of Strobilanthes.

Lindernia rotundifolia (L.) Alston (Linderniaceae), Picria fel-terrae Lour. (Linderniaceae), and Limnophila aromatica (Lam.) Merr. (Plantaginaceae) are newly reported from Jalthal forest, eastern Nepal. Picria Lour. is a new generic record for Nepal. Descriptions of all the species based on Nepalese specimens are provided, along with notes on diagnostic features, color photographs of the species, distribution maps, and notes on habitats. [ABSTRACT FROM AUTHOR]

Agalinis (Orobanchaceae, tribe Pedicularideae) consists of approximately 70 hemiparasitic species distributed among North, Central, and South America. Previous phylogenetic studies did not include tropical species nor comprehensive sampling for closely allied genera, limiting our systematic understanding of these lineages. We generated an updated phylogenetic hypothesis from 179 individuals from 51 species of Agalinis and five genera once considered congeneric with Agalinis (Anisantherina, Brachystigma, Dasistoma, Aureolaria, and Esterhazya), particularly focusing on sampling underrepresented Central and South American taxa. Phylogenetic analyses were based on six cpDNA regions (rbcL, matK, trnT(UGU)-trnF(GAA), rps2, rpoB, and psbA-trnH) and four nuclear regions (ITS, PPR-AT1G09680, PPR-AT3G09060, and PPR-AT5G39980) and were conducted using maximum likelihood and Bayesian inference methods. Additionally, our data were added to previously published Orobanchaceae-wide datasets to reveal placement of these lineages in a comprehensive phylogenetic context using maximum likelihood. Our results reveal a strongly supported South American Agalinis clade that is sister to taxa from southwestern North America and Mexico and includes distinct Brazilian and Andean clades. Esterhazya, a hummingbird-pollinated genus in Brazil, is nested within the Brazilian Agalinis clade with strong support. We confirm well-supported incongruences between chloroplast and nuclear partitions, particularly concerning placement of early-diverging lineages within Agalinis. The monotypic genus Anisantherina, once included in Agalinis, is resolved within Orobanchaceae tribe Buchnereae, a placement that is further corroborated by anther morphology. Orobanchaceae consist of numerous species from tropical regions that have not been included in phylogenetic studies, and our study underscores the importance of more comprehensive sampling for the placement of these lineages to clarify taxonomy, biogeography, and character evolution. [ABSTRACT FROM AUTHOR]

Strobilanthes dalzielii of Acanthaceae is an herb species with potentially extensive applications for its pharmaceutical and ornamental values. Due to taxonomic complications and limited genetic information, the structural characteristics, and phylogenetic relationships of the S. dalzielii chloroplast genome were assembled and characterized here for the first time. The complete chloroplast genome of S. dalzielii was 144,580 bp in length. The genome is quadripartite in structure and consists of a large single-copy region (92,137 bp) and a small single-copy region (17,669 bp), which are separated by a pair of inverted repeats (each 17,387 bp). A total of 125 genes were annotated, including 80 protein-coding, 37 transfer RNA, and eight ribosomal RNA genes. The overall GC content was 36.4%. Phylogenetic analysis based on the complete chloroplast genome sequence of 21 taxa within the tribe Ruellieae of Acanthaceae using the maximum likelihood and Bayesian inference methods revealed that Strobilanthes diverged after Ruellia; S. dalzielii is closely related to S. tonkinensis. The genomic data obtained from this study will serve as valuable information to the species delimitation and genetic classification of Strobilanthes. [ABSTRACT FROM AUTHOR]

Utilising both morphological and molecular analyses, this study unveils Mazus jiangshiense sp. nov., a novel addition to the Mazaceae family, discovered in Shaowu County, Fujian Province, China. The comprehensive description and illustrations provided here are a result of a meticulous exploration of its morphological features. While bearing a resemblance to M. gracilis, this new-found species is distinguished by three distinct characteristics: its stems are delicately soft, its leaves possess a membranous quality and the ovary is notably villous at the apex. Integration of molecular evidence, derived from the nuclear ribosomal DNA (nrITS) and three plastid DNA sequences (rps16, rbcL and trnL-trnF), unequivocally supports the classification of M. jiangshiense as a distinct species. Notably, the molecular analysis positions it as a sister species to M. spicatus, underscoring the phylogenetic relationships within the genus Mazus. Our research not only introduces M. jiangshiense as a novel taxonomic entity, but also provides a nuanced understanding of its morphological differences and molecular affinities, enriching our comprehension of the diversity and evolutionary relationships of Mazaceae. [ABSTRACT FROM AUTHOR]

Acanthaceae está entre as 12 famílias de angiospermas mais diversas, representada por 190 gêneros e estimadas 4.900 espécies, com distribuição pantropical. O respectivo trabalho realizou um levantamento florístico das Acanthaceae no município de Vitória da Conquista, Bahia, região de tensão ecológica entre Caatinga e Mata Atlântica. Foram realizadas 28 expedições durante o período de dezembro/2021 a outubro/2023, abrangendo todo o município, além de duas coletas extras em abril/2024. Essas coletas foram quinzenais (fora da época de floração) e semanais (época de floração), e totalizaram 48 espécimes amostrados, depositados nos herbários HUESBVC e HVC. Foram registradas 14 espécies para o município, sendo elas: Aphelandra marginata, Clistax speciosus, Dicliptera ciliaris, Justicia laevilinguis, Lepidagathis nemoralis, Poikilacanthus bahiensis, Ruellia bahiensis, R. brevicaulis, R. brevifolia, R. ciliatiflora, R. geminiflora, R. paniculata, Ruellia sp. e Thyrsacanthus ramosissimus. Das espécies coletadas somente D. ciliaris, R. brevifolia e R. paniculata não são endêmicas do Brasil e nove são novos registros para o município. Nossos resultados agregam conhecimento sobre a flora de Vitória da Conquista e, consequentemente, da Bahia e ampliam o acervo dos herbários locais.

The genus Justicia (Acanthaceae) is represented by 12 species in the Mexican portion of the Yucatan Peninsula (MYP), one of which, Justicia cardinalis, is herein proposed as new to science. The new species is known from sub-xerophytic or seasonally dry forests over calcareous, rocky soils along the northern and northeastern regions in the MYP. This taxon has been confused with J. carthagenensis for more than 100 yr, but there are remarkable differences between the two taxa. The new species has linear to narrowly obovate (vs. oblong-spathulate to spathulate) floral bracts and narrower leaves. The corolla is red with white markings in the lower lip (vs. purple with white markings on the central lower lip and the upper lobes), and the corolla tube is as long as the lobes, whereas it is shorter than the lobes in J. carthagenensis. We provide relevant visual resources to aid in the identification of the new species. The conservation status is preliminarily assessed as Least Concern (LC) using the B criteria of the IUCN. Furthermore, we provide a brief synopsis, a distribution map, and a key to the species of Justicia from the MYP (as well as photographs of most of them). Finally, to be able to apply the names in an unambiguous manner, we designate a lectotype and an epitype for J. carthagenensis, and a lectotype for J. spicigera. [ABSTRACT FROM AUTHOR]

We present a genome assembly from an individual Scutellaria minor (Tracheophyta; Magnoliopsida; Lamiales; Lamiaceae). The genome sequence is 341.8 megabases in span. Most of the assembly is scaffolded into 14 chromosomal pseudomolecules. The mitochondrial and plastid genome assemblies have lengths of 376.64 kilobases and 152.59 kilobases in length, respectively.

Phylogenetic relationships of Scrophulariaceae s. l.（sensu lato）and the closely related groups were explored with 129 plastid genomes representing 107 genera. Plastome sequences were downloaded from the GenBank. Based on coding sequences (CDS), phylogenetic trees were reconstructed using maximum likelihood（ML）and Bayesian inference（BI）approaches. The ML and BI analyses shared the same topology and the main clades were consistently recovered with high support. Of the 129 total nodes, 123 were supported by ML bootstrap value ≥70%. The result were as follows：（1）Scrophulariaceae s. l. was confirmed as polyphyletic. Fifty-one species with representatives of 37 genera from the Scrophulariaceae s.l. were assigned to seven families. These were Orobanchaceae, Paulowniaceae, Wightiaceae, Mazaceae, Linderniaceae, Scrophulariaceae s. s.（sensu stricto）, and Plantaginaceae.（2）Scrophulariaceae s.s. was a monophyletic group. In addition to the recognition of eight genera（including Bontia, Calamphoreus, Diocirea, Eremophila, Glycocystis, Leucophyllum, Scrophularia and Verbascum）comprised primarily of former members of Scrophulariaceae s.l., Buddleja which was originally placed in Loganiaceae, and Myoporum, traditionally placed in the Myoporaceae had been demonstrated to belong to this clade.（3）The Lamiales was monophyletic. Fourteen well-supported monophyletic clades were obtained corresponding to each family (Wightiaceae and Pedaliaceae for which only one species had been contained were not included). The relationships among the families were well resolved. Oleaceae formed a well-supported clade that was distinct from core Lamiales. Within the core Lamiales, four clades (Gesneriaceae, Plantaginaceae, Scrophulariaceae, and Linderniaceae) diverged before the remaining families grouping in two sister clades. Within one clade, two moderately to highly supported subclades were recovered. Lentibulariaceae was resolved as sister group to Acanthaceae, and Pedaliaceae was sister to Verbenaceae + Bignoniaceae. The other clade contained six families, with Lamiaceae, Mazaceae, Wightiaceae + Phrymaceae, and Paulowniaceae successively sister to Orobanchaceae. The results of our analyses indicate that the traditional Scrophulariaceae are an unnatural assemblage of plants distributed throughout the phylogenentic tree of Lamiales. Phylogenomics analyses based on plastid organelle have shown good potential for phylogenetic inference in Scrophulariaceae, more taxonomic sampling are needed to enhance our understanding of phylogenetic relationships of Scrophulariaceae in future studies.

Oreocharis argyreia var. angustifolia of Gesneriaceae is widely distributed in South China, including Guangdong, Guangxi, Hunan, and Jiangxi provinces. However, genetic information of this species is limited, further contributing to the taxonomic complications surrounding this species. Thus, in this study, we assembled and characterized the complete chloroplast genome of O. argyreia var. angustifolia as a genomic resource for future studies. The complete plastid genome was 154,675 bp in size, with a pair of inverted repeat regions of 25,329 bp each, separating the 85,977-bp large and 18,040-bp small single copy regions. A total of 131 genes were predicted, consisting of 86 protein-coding, 37 tRNA, and eight rRNA genes. The overall GC content was 37.6%. Phylogenetic analysis based on 79 shared unique CDS resulted in a fully resolved phylogenetic tree using both the maximum likelihood and Bayesian inference methods. Based on current circumscription, both methods indicated that Oreocharis is monophyletic; O. argyreia var. angustifolia diverged after O. chienii, which then followed by the divergence of the other three species included namely, O. continifolia, O. esquirolii, and O. mileensis. The genomic data obtained will be useful for future studies on the phylogenetics and evolution of Gesneriaceae.

Verbascoside and isoverbascoside are two active phenylethanoid glycosides mainly found in plants of the order Lamiales. This study analyzes the verbascoside and isoverbascoside levels and the total phenolic contents in the water and ethanolic extracts of 20 medicinal plants of the order Lamiales commonly used in Thailand. The related bioactivities, including the antioxidant activity via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reduction activity potential assays and anti-tyrosinase and -inflammatory activities via the cyclooxygenase and nitric oxide assays are also investigated. The extracts of several plant species, including Barleria prionitis, B. lupulina, Rhinacanthus nasutus, Orthosiphon aristatus, and Nicoteba betonica, exhibit high verbascoside and isoverbascoside content levels. The correlation analysis between the bioactive activities and the active compounds demonstrates a significant association between the verbascoside level in the water extracts and both the DPPH antioxidant activity and the nitric oxide level in the anti-inflammatory assays. This study provides the first report on the verbascoside and isoverbascoside quantification of several plant samples. The findings provide valuable insights for future research on lesser-studied plants possessing high verbascoside and isoverbascoside levels, which exhibit promising anti-inflammatory activities.

Premise: The genus Calceolaria (Calceolariaceae) is emblematic of the Andes, is hypothesized to have originated as a recent, rapid radiation, and has important taxonomic needs. Additionally, the genus is a model for the study of specialized pollination systems, as its flowers are nectarless and many offer floral oils as a pollination reward collected by specialist bees. Despite their evolutionary and ecological significance, obtaining a resolved phylogeny for the group has proved difficult. To address this challenge, we present a new bait set for targeted sequencing of nuclear loci in Calceolariaceae and close relatives. Methods: We developed a bioinformatic workflow to use incomplete, low‐coverage genomes of 10 Calceolaria species to identify single‐copy loci suitable for phylogenetic studies and design baits for targeted sequencing. Results: Our approach resulted in the identification of 809 single‐copy loci (733 noncoding and 76 coding regions) and the development of 39,937 baits, which we validated in silico (10 specimens) and in vitro (29 Calceolariaceae and six outgroups). In both cases, the data allowed us to recover robust phylogenetic estimates. Discussion: Our results demonstrate the appropriateness of the bait set for sequencing recent and historic specimens of Calceolariaceae and close relatives, and open new doors for further investigation of the evolutionary history of this hyperdiverse genus. [ABSTRACT FROM AUTHOR]

A taxonomic revision of Fernandoa Welw. ex Seem. (Bignoniaceae) in Thailand is presented. Two species, F. adenophylla (Wall. ex G. Don) Steenis and F. collignonii (Dop) Steenis, are enumerated with updated morphological descriptions, illustrations and a taxonomic identification key, together with notes on distributions, distribution maps, habitats and ecology, phenology, conservation assessments, etymology, vernacular names, uses, and specimens examined. The collection of Wallich Cat. 6502A from Myanmar, Ava at G [G00133642] is designated here as the lectotype of F. adenophylla in a second step lectotypification. F. collignonii has a conservation status of Endangered [EN]. The leaf, stem, and wood anatomy and pollen morphology of F. adenophylla are also reported in this study. [ABSTRACT FROM AUTHOR]

Phylogenetic relationships of Scrophulariaceae s. l.(sensu lato)and the closely related groups were explored with 129 plastid genomes representing 107 genera. Plastome sequences were downloaded from the GenBank. Based on coding sequences(CDS), phylogenetic trees were reconstructed using maximum likelihood(ML)and Bayesian inference(BI)approaches. The ML and BI analyses shared the same topology and the main clades were consistently recovered with high support. Of the 129 total nodes, 123 were supported by ML bootstrap value ≥70%. The result were as follows:(1)Scrophulariaceae s. l. was confirmed as polyphyletic. Fifty-one species with representatives of 37 genera from the Scrophulariaceae s.l. were assigned to seven families. These were Orobanchaceae, Paulowniaceae, Wightiaceae, Mazaceae, Linderniaceae, Scrophulariaceae s. s.(sensu stricto), and Plantaginaceae.(2)Scrophulariaceae s.s. was a monophyletic group. In addition to the recognition of eight genera(including Bontia, Calamphoreus, Diocirea, Eremophila, Glycocystis, Leucophyllum, Scrophularia and Verbascum)comprised primarily of former members of Scrophulariaceae s.l., Buddleja which was originally placed in Loganiaceae, and Myoporum, traditionally placed in the Myoporaceae had been demonstrated to belong to this clade.(3)The Lamiales was monophyletic. Fourteen well-supported monophyletic clades were obtained corresponding to each family(Wightiaceae and Pedaliaceae for which only one species had been contained were not included). The relationships among the families were well resolved. Oleaceae formed a well-supported clade that was distinct from core Lamiales. Within the core Lamiales, four clades(Gesneriaceae, Plantaginaceae, Scrophulariaceae, and Linderniaceae)diverged before the remaining families grouping in two sister clades. Within one clade, two moderately to highly supported subclades were recovered. Lentibulariaceae was resolved as sister group to Acanthaceae, and Pedaliaceae was sister to Verbenaceae + Bignoniaceae. The other clade contained six families, with Lamiaceae, Mazaceae, Wightiaceae + Phrymaceae, and Paulowniaceae successively sister to Orobanchaceae. The results of our analyses indicate that the traditional Scrophulariaceae are an unnatural assemblage of plants distributed throughout the phylogenentic tree of Lamiales. Phylogenomics analyses based on plastid organelle have shown good potential for phylogenetic inference in Scrophulariaceae, more taxonomic sampling are needed to enhance our understanding of phylogenetic relationships of Scrophulariaceae in future studies. [ABSTRACT FROM AUTHOR]

Plastid molecular phylogenies that broadly sampled angiosperm lineages imply that carnivorous plants evolved at least 11 times independently in 13 families and 6 orders. Within and between these clades, the different prey capture strategies involving flypaper and pitfall structures arose in parallel with the subsequent evolution of snap traps and suction bladders. Attempts to discern the deep ontological history of carnivorous structures using multigene phylogenies have provided a plastid-level picture of sister relationships at the family level. Here, we present a molecular phylogeny of the angiosperms based on nuclear target sequence capture data (Angiosperms-353 probe set), assembled by the Kew Plant Trees of Life initiative, which aims to complete the tree of life for plants. This phylogeny encompasses all carnivorous and protocarnivorous families, although certain genera such as Philcoxia (Plantaginaceae) are excluded. This study offers a novel nuclear gene-based overview of relationships within and between carnivorous families and genera. Consistent with previous broadly sampled studies, we found that most carnivorous families are not affiliated with any single family. Instead, they emerge as sister groups to large clades comprising multiple non-carnivorous families. Additionally, we explore recent genomic studies across various carnivorous clades that examine the evolution of the carnivorous syndrome in relation to whole-genome duplication, subgenome dominance, small-scale gene duplication, and convergent evolution. Furthermore, we discuss insights into genome size evolution through the lens of carnivorous plant genomes. [ABSTRACT FROM AUTHOR]

Oreocharis argyreia var. angustifolia of Gesneriaceae is widely distributed in South China, including Guangdong, Guangxi, Hunan, and Jiangxi provinces. However, genetic information of this species is limited, further contributing to the taxonomic complications surrounding this species. Thus, in this study, we assembled and characterized the complete chloroplast genome of O. argyreia var. angustifolia as a genomic resource for future studies. The complete plastid genome was 154,675 bp in size, with a pair of inverted repeat regions of 25,329 bp each, separating the 85,977-bp large and 18,040-bp small single copy regions. A total of 131 genes were predicted, consisting of 86 protein-coding, 37 tRNA, and eight rRNA genes. The overall GC content was 37.6%. Phylogenetic analysis based on 79 shared unique CDS resulted in a fully resolved phylogenetic tree using both the maximum likelihood and Bayesian inference methods. Based on current circumscription, both methods indicated that Oreocharis is monophyletic; O. argyreia var. angustifolia diverged after O. chienii, which then followed by the divergence of the other three species included namely, O. continifolia, O. esquirolii, and O. mileensis. The genomic data obtained will be useful for future studies on the phylogenetics and evolution of Gesneriaceae. [ABSTRACT FROM AUTHOR]

Allopolyploid plants have long been regarded as possessing genetic advantages under certain circumstances due to the combined effects of their hybrid origins and duplicated genomes. However, the evolutionary consequences of allopolyploidy in lineage diversification remain to be fully understood. Here, we investigate the evolutionary consequences of allopolyploidy using 138 transcriptomic sequences of Gesneriaceae, including 124 newly sequenced, focusing particularly on the largest subtribe Didymocarpinae. We estimated the phylogeny of Gesneriaceae using concatenated and coalescent-based methods based on five different nuclear matrices and 27 plastid genes, focusing on relationships among major clades. To better understand the evolutionary affinities in this family, we applied a range of approaches to characterize the extent and cause of phylogenetic incongruence. We found that extensive conflicts between nuclear and chloroplast genomes and among nuclear genes were caused by both incomplete lineage sorting (ILS) and reticulation, and we found evidence of widespread ancient hybridization and introgression. Using the most highly supported phylogenomic framework, we revealed multiple bursts of gene duplication throughout the evolutionary history of Gesneriaceae. By incorporating molecular dating and analyses of diversification dynamics, our study shows that an ancient allopolyploidization event occurred around the Oligocene–Miocene boundary, which may have driven the rapid radiation of core Didymocarpinae. [ABSTRACT FROM AUTHOR]

Abstract Objectives Petrea volubilis, a member of the Order Lamiales and the Verbenaceae family, is an important horticultural species that has been used in traditional folk medicine. To provide a genome sequence for comparative studies within the Order Lamiales that includes important families such as Lamiaceae (mints), we generated a long-read, chromosome-scale genome assembly of this species. Data description Using a total of 45.5 Gb of Pacific Biosciences long read sequence, we generated a 480.2 Mb assembly of P. volubilis, of which, 93% is chromosome anchored. Representation of genic regions was robust with 96.6% of the Benchmarking of Universal Single Copy Orthologs present in the genome assembly. A total of 57.8% of the genome was annotated as a repetitive sequence. Using a gene annotation pipeline that included refinement of gene models using transcript evidence, 30,982 high confidence genes were annotated. Access to the P. volubilis genome will facilitate evolutionary studies in the Lamiales, a key order of Asterids that includes significant crop and medicinal plant species.

Abstract Premise The genus Calceolaria (Calceolariaceae) is emblematic of the Andes, is hypothesized to have originated as a recent, rapid radiation, and has important taxonomic needs. Additionally, the genus is a model for the study of specialized pollination systems, as its flowers are nectarless and many offer floral oils as a pollination reward collected by specialist bees. Despite their evolutionary and ecological significance, obtaining a resolved phylogeny for the group has proved difficult. To address this challenge, we present a new bait set for targeted sequencing of nuclear loci in Calceolariaceae and close relatives. Methods We developed a bioinformatic workflow to use incomplete, low‐coverage genomes of 10 Calceolaria species to identify single‐copy loci suitable for phylogenetic studies and design baits for targeted sequencing. Results Our approach resulted in the identification of 809 single‐copy loci (733 noncoding and 76 coding regions) and the development of 39,937 baits, which we validated in silico (10 specimens) and in vitro (29 Calceolariaceae and six outgroups). In both cases, the data allowed us to recover robust phylogenetic estimates. Discussion Our results demonstrate the appropriateness of the bait set for sequencing recent and historic specimens of Calceolariaceae and close relatives, and open new doors for further investigation of the evolutionary history of this hyperdiverse genus.

We present a genome assembly from a specimen of Ballota nigra (black horehound; Tracheophyta; Magnoliopsida; Lamiales; Lamiaceae). The genome sequence is 1186.8 megabases in span. Most of the assembly is scaffolded into 11 chromosomal pseudomolecules. Three mitochondrial chromosomes were assembled, with lengths of 148,17, 121,67 and 125,74 kilobases. The chloroplast genome has been assembled and is 151.91 kilobases in length.

In this paper we describe and illustrate Schlegelia longirachis a new species from montane forest remnants (1200--1900 m) in the Western slope of the Eastern Cordillera of Colombia (“Serranía de Las Quinchas” and Virolín county) in the Departments of Boyacá and Santander. A root-climbing liana, the new species is contrasted to S. fuscata, S. monachinoi and S. parviflora, the three most morphologically similar species of Schlegelia. This new species is differentiated from its putative close relatives by vegetative (texture, colour, pubescence and shape in leaves, bracts, bracteoles pedicel, calyx and corolla), inflorescences as well as floral characters (staminode absent). We provide an updated key to 24 known species of Schlegelia. For the identification key, S. fuscata and S. roseiflora are regarded here as different from S. parviflora. S. urbaniana is considered a synonym of S. axillaris, whereas S. fastigiata is separated from S. sulphurea as a recognizable species. Schlegelia has its center of distribution in Colombia, where 17 of the species are known to occur. [ABSTRACT FROM AUTHOR]

Our previous study was the first to confirm that the predominant conformation of mitochondrial genome (mitogenome) sequence of Salvia species contains two circular chromosomes. To further understand the organization, variation, and evolution of Salvia mitogenomes, we characterized the mitogenome of Salvia officinalis. The mitogenome of S. officinalis was sequenced using Illumina short reads and Nanopore long reads and assembled using a hybrid assembly strategy. We found that the predominant conformation of the S. officinalis mitogenome also had two circular chromosomes that were 268,341 bp (MC1) and 39,827 bp (MC2) in length. The S. officinalis mitogenome encoded an angiosperm-typical set of 24 core genes, 9 variable genes, 3 rRNA genes, and 16 tRNA genes. We found many rearrangements of the Salvia mitogenome through inter- and intra-specific comparisons. A phylogenetic analysis of the coding sequences (CDs) of 26 common protein-coding genes (PCGs) of 11 Lamiales species and 2 outgroup taxa strongly indicated that the S. officinalis was a sister taxon to S. miltiorrhiza, consistent with the results obtained using concatenated CDs of common plastid genes. The mapping of RNA-seq data to the CDs of PCGs led to the identification of 451 C-to-U RNA editing sites from 31 PCGs of the S. officinalis mitogenome. Using PCR amplification and Sanger sequencing methods, we successfully validated 113 of the 126 RNA editing sites from 11 PCGs. The results of this study suggest that the predominant conformation of the S. officinalis mitogenome are two circular chromosomes, and the stop gain of rpl5 was found through RNA editing events of the Salvia mitogenome. [ABSTRACT FROM AUTHOR]

Objectives : Petrea volubilis, a member of the Order Lamiales and the Verbenaceae family, is an important horticultural species that has been used in traditional folk medicine. To provide a genome sequence for comparative studies within the Order Lamiales that includes important families such as Lamiaceae (mints), we generated a long-read, chromosome-scale genome assembly of this species. Data description: Using a total of 45.5 Gb of Pacific Biosciences long read sequence, we generated a 480.2 Mb assembly of P. volubilis, of which, 93% is chromosome anchored. Representation of genic regions was robust with 96.6% of the Benchmarking of Universal Single Copy Orthologs present in the genome assembly. A total of 57.8% of the genome was annotated as a repetitive sequence. Using a gene annotation pipeline that included refinement of gene models using transcript evidence, 30,982 high confidence genes were annotated. Access to the P. volubilis genome will facilitate evolutionary studies in the Lamiales, a key order of Asterids that includes significant crop and medicinal plant species. [ABSTRACT FROM AUTHOR]

We present a genome assembly from a Linaria vulgaris specimen (the common toadflax; Streptophyta; Magnoliopsida; Lamiales; Plantaginaceae). The genome sequence is 760.5 megabases in span. Most of the assembly is scaffolded into six chromosomal pseudomolecules. Two mitochondrial genomes were assembled, which were 330.8 and 144.0 kilobases long. The plastid genome was also assembled and is 156.7 kilobases in length.

While native populations are often adapted to historical biotic and abiotic conditions at their home site, populations from other locations in the range may be better adapted to current conditions due to changing climates or extreme conditions in a single year. We examine whether local populations of a widespread species maintain a relative advantage over distant populations that have evolved at sites better matching the current climate. Specifically, we grew lines derived from low- and high-elevation annual populations in California and Oregon of the common monkeyflower (Erythranthe guttata) and conducted phenotypic selection analyses in low- and high-elevation common gardens in Oregon to examine relative fitness and the traits mediating relative fitness. Californian low-elevation populations have the highest relative fitness at the low-elevation site, and Californian high-elevation populations have the highest relative fitness at the high-elevation site. Relative fitness differences are mediated by selection for properly timed transitions to flowering, with selection favoring more rapid growth rates at the low-elevation site and greater vegetative biomass prior to flowering at the high-elevation site. Fitness advantages for Californian plants occur despite incurring higher herbivory at both sites than the native Oregonian plants. Our findings suggest that a lag in adaptation causes maladaptation in extreme years that may be more prevalent in future climates, but local populations still have high growth rates and thus are not yet threatened.

Abstract Verbenaceae includes 32 genera and approximately 800 species distributed mainly in the Neotropical region, especially diversified in Brazil, where the campo rupestre stands out as an important vegetation type for the family. The Itacolomi State Park (ISP) is located in the southeast of the Quadrilátero Ferrífero, Minas Gerais state (MG), Brazil. Vegetation at the Park is composed of campo rupestre and forest remnants among degraded areas. The present research provides a floristic treatment of Verbenaceae in this protected area. Data were obtained from fieldwork and herbarium study. A total of 13 species were recorded: Glandularia phlogiflora, Lantana camara, Lantan fucata, Lantana tiliaefolia, Lantana trifolia, Lippia brasiliensis, Lippia hermannioides, Lippia origanoides, Petrea volubilis, Stachytarpheta cayennensis, Stachytarpheta commutata, Verbena litoralis and Verbena rigida. Among them, six are new records for the ISP. Stachytarpheta glabra, endemic to MG, was found in an area of canga very close to the boundaries of the Park. Additionally, S. viscidula, whose type locality is close to the Park, is proposed as a new synonym for S. commutata, whose type specimen comes from the Park. We provide an identification key, descriptions, photographs and comments on taxonomy, ecology and distribution for each species.

Phlomis lychnitis se registra por primera vez en el continente africano. Hasta el momento, sólo se conoce de Marruecos en el Alto Atlas Oriental. Se proporciona una descripción de la especie, así como los primeros datos sobre su ecología en Marruecos junto con su distribución geográfica y caracteres diagnósticos con la especie morfológicamente más cercana que comparte flores de color amarillo. También se proporcionan fotografías y una clave actualizada de las especies de Phlomis en Marruecos.

Abstract This study presents a taxonomic survey of the species of Acanthaceae in the Cuiabá lowlands, an area encompassing several habitats, from rock outcrops in Chapada dos Guiamarães to the periodically flooded forest areas in the Pantanal. Herbarium collections were analyzed and 13 field expeditions were carried out to visit different phytophysiognomies and ecosystems. The family is represented by 31 species and 12 genera, the most diverse being Justicia (10 species) and Ruellia (eight species). Additionally, Dicliptera squarrosa, Dyschoriste schottiana, Ruellia blechum, R. jussieuoides, and R. trachyphylla are newly recorded in the state of Mato Grosso. The number of species is higher if compared with other taxonomic studies of Acanthaceae in Central-Western Brazil. Despite the area being overlooked by the botanical community, we expect our results will instigate further taxonomic studies in the area. This study provides an identification key, short descriptions, comments on taxonomy and distribution, and photographs.

With the disintegration of the traditionally circumscribed Scrophulariaceae, the phylogenetic placements of most genera formerly placed in that family have been resolved. However, the familial placement of the monotypic genus Mimulicalyx has not been included in any published molecular phylogenetic study. Here, we adopt a two-step approach to resolve the phylogenetic placement of Mimulicalyx using three datasets: (1) a 71 619 bp plastome alignment of Lamiales; (2) a dataset of six plastid DNA regions (atpB, matK, ndhF, rbcL, rps16 and trnL-trnF) for Phrymaceae; and (3) a dataset of the nuclear ribosomal internal and external transcribed spacer regions (nrITS, ETS) for Phrymaceae. Our analyses reveal that Mimulicalyx is a member of the tribe Mimuleae in Phrymaceae. Generic level phylogenetic relationships within Phrymaceae are further discussed, and a dichotomous key to the 15 genera currently recognized is provided. [ABSTRACT FROM AUTHOR]

This study presents a taxonomic survey of the species of Acanthaceae in the Cuiabá lowlands, an area encompassing several habitats, from rock outcrops in Chapada dos Guiamarães to the periodically flooded forest areas in the Pantanal. Herbarium collections were analyzed and 13 field expeditions were carried out to visit different phytophysiognomies and ecosystems. The family is represented by 31 species and 12 genera, the most diverse being Justicia (10 species) and Ruellia (eight species). Additionally, Dicliptera squarrosa, Dyschoriste schottiana, Ruellia blechum, R. jussieuoides, and R. trachyphylla are newly recorded in the state of Mato Grosso. The number of species is higher if compared with other taxonomic studies of Acanthaceae in Central-Western Brazil. Despite the area being overlooked by the botanical community, we expect our results will instigate further taxonomic studies in the area. This study provides an identification key, short descriptions, comments on taxonomy and distribution, and photographs. [ABSTRACT FROM AUTHOR]

Verbenaceae includes 32 genera and approximately 800 species distributed mainly in the Neotropical region, especially diversified in Brazil, where the campo rupestre stands out as an important vegetation type for the family. The Itacolomi State Park (ISP) is located in the southeast of the Quadrilátero Ferrífero, Minas Gerais state (MG), Brazil. Vegetation at the Park is composed of campo rupestre and forest remnants among degraded areas. The present research provides a floristic treatment of Verbenaceae in this protected area. Data were obtained from fieldwork and herbarium study. A total of 13 species were recorded: Glandularia phlogiflora, Lantana camara, Lantan fucata, Lantana tiliaefolia, Lantana trifolia, Lippia brasiliensis, Lippia hermannioides, Lippia origanoides, Petrea volubilis, Stachytarpheta cayennensis, Stachytarpheta commutata, Verbena litoralis and Verbena rigida. Among them, six are new records for the ISP. Stachytarpheta glabra, endemic to MG, was found in an area of canga very close to the boundaries of the Park. Additionally, S. viscidula, whose type locality is close to the Park, is proposed as a new synonym for S. commutata, whose type specimen comes from the Park. We provide an identification key, descriptions, photographs and comments on taxonomy, ecology and distribution for each species. [ABSTRACT FROM AUTHOR]

Plastid molecular phylogenies that broadly sampled angiosperm lineages imply that carnivorous plants evolved at least 11 times independently in 13 families and 6 orders. Within and between these clades, the different prey capture strategies involving flypaper and pitfall structures arose in parallel with the subsequent evolution of snap traps and suction bladders. Attempts to discern the deep ontological history of carnivorous structures using multigene phylogenies have provided a plastid-level picture of sister relationships at the family level. Here, we present a molecular phylogeny of the angiosperms based on nuclear target sequence capture data (Angiosperms-353 probe set), assembled by the Kew Plant Trees of Life initiative, which aims to complete the tree of life for plants. This phylogeny encompasses all carnivorous and protocarnivorous families, although certain genera such as Philcoxia (Plantaginaceae) are excluded. This study offers a novel nuclear gene-based overview of relationships within and between carnivorous families and genera. Consistent with previous broadly sampled studies, we found that most carnivorous families are not affiliated with any single family. Instead, they emerge as sister groups to large clades comprising multiple non-carnivorous families. Additionally, we explore recent genomic studies across various carnivorous clades that examine the evolution of the carnivorous syndrome in relation to whole-genome duplication, subgenome dominance, small-scale gene duplication, and convergent evolution. Furthermore, we discuss insights into genome size evolution through the lens of carnivorous plant genomes.

Abstract Background An outstanding question in evolutionary biology is how genetic interactions defining novel traits evolve. They may evolve either by de novo assembly of previously non-interacting genes or by en bloc co-option of interactions from other functions. We tested these hypotheses in the context of a novel phenotype—Lamiales flower monosymmetry—defined by a developmental program that relies on regulatory interaction among CYCLOIDEA, RADIALIS, DIVARICATA, and DRIF gene products. In Antirrhinum majus (snapdragon), representing Lamiales, we tested whether components of this program likely function beyond their previously known role in petal and stamen development. In Solanum lycopersicum (tomato), representing Solanales which diverged from Lamiales before the origin of Lamiales floral monosymmetry, we additionally tested for regulatory interactions in this program. Results We found that RADIALIS, DIVARICATA, and DRIF are expressed in snapdragon ovaries and developing fruit, similar to their homologs during tomato fruit development. In addition, we found that a tomato CYCLOIDEA ortholog positively regulates a tomato RADIALIS ortholog. Conclusion Our results provide preliminary support to the hypothesis that the developmental program defining floral monosymmetry in Lamiales was co-opted en bloc from a function in carpel development. This expands our understanding of novel trait evolution facilitated by co-option of existing regulatory interactions.

Lamiaceae Martinov é a sexta maior família das Angiospermas, contendo cerca de 240 gêneros e 7.200 espécies. No Brasil, também apresenta alta riqueza, representada por 70 gêneros e aproximadamente 590 espécies. Apesar disso, é uma família relativamente pouco representada em estudos florísticos regionais. O objetivo deste trabalho foi realizar a florística e taxonomia das espécies de Lamiaceae ocorrentes no município de Alagoinhas, Bahia, Brasil. O levantamento foi realizado tanto por coletas do material botânico, quanto por consulta em materiais depositados em acervos de herbários da região como o HUNEB e o HUEFS. A família está representada no município por 16 espécies distribuídas em 13 gêneros e 4 tribos. Dentre essas espécies, se destaca Mesosphaerum caatingense, que teve seu primeiro registro para o estado da Bahia e para a Mata Atlântica realizado neste trabalho. São apresentadas descrições, fotografias de campo, comentários sobre as espécies e uma chave de identificação.

Salvia miltiorrhiza has been an economically important medicinal plant. Previously, an S. miltiorrhiza mitochondrial genome (mitogenome) assembled from Illumina short reads, appearing to be a single circular molecule, has been published. Based on the recent reports on the plant mitogenome structure, we suspected that this conformation does not accurately represent the complexity of the S. miltiorrhiza mitogenome. In the current study, we assembled the mitogenome of S. miltiorrhiza using the PacBio and Illumina sequencing technologies. The primary structure of the mitogenome contained two mitochondrial chromosomes (MC1 and MC2), which corresponded to two major conformations, namely, Mac1 and Mac2, respectively. Using two approaches, including (1) long reads mapping and (2) polymerase chain reaction amplification followed by Sanger sequencing, we observed nine repeats that can mediate recombination. We predicted 55 genes, including 33 mitochondrial protein-coding genes (PCGs), 3 rRNA genes, and 19 tRNA genes. Repeat analysis identified 112 microsatellite repeats and 3 long-tandem repeats. Phylogenetic analysis using the 26 shared PCGs resulted in a tree that was congruent with the phylogeny of Lamiales species in the APG IV system. The analysis of mitochondrial plastid DNA (MTPT) identified 16 MTPTs in the mitogenome. Moreover, the analysis of nucleotide substitution rates in Lamiales showed that the genes atp4, ccmB, ccmFc, and mttB might have been positively selected. The results lay the foundation for future studies on the evolution of the Salvia mitogenome and the molecular breeding of S. miltiorrhiza. [ABSTRACT FROM AUTHOR]

Lamiaceae are one of the largest and most economically important families of flowering plants. Despite focused study on relationships within subclades, higher-level relationships have been under-studied. Moreover, the herbaceous habit of much of the family has resulted in a poor fossil record and has hampered estimates of divergence times. Using a new dataset of five plastid loci from 178 members of Lamiaceae representing all subfamilies and nearly all tribes, we clarify major infrafamilial relationships and present a robust set of divergence times. We use this phylogenetic hypothesis as a platform to test previous hypotheses regarding the historical biogeography and evolution of major traits in the family. We confirm the placement of subfamily Nepetoideae, show continued uncertainty in the placement of subfamilies Ajugoideae and Premnoideae and highlight extreme discordance with recent results from nuclear data. Lamiaceae originated during the Late Cretaceous as woody plants with nutlet fruits and four stamens, probably in South-East Asia. Most subfamilies diverged during the Eocene, perhaps facilitated by climatic cooling. Our results provide a valuable set of secondary dates for Lamiaceae and highlight the need for focused study of subfamilies Callicarpoideae and Viticoideae. Our results also provide several hypotheses regarding trait or range-dependent diversification. [ABSTRACT FROM AUTHOR]

RESUMO Apresentamos o tratamento florístico de Verbenaceae no Parque Estadual do Biribiri, localizado no Planalto de Diamantina, Cadeia do Espinhaço, Estado de Minas Gerais. Com extensão atual de 16.998,66 ha, essa Unidade de Conservação estadual integra uma área de importância biológica especial, com vegetação representada predominantemente por campos rupestres. Após o estudo de campo e consulta a herbários nós encontramos 14 táxons de Verbenaceae incluídos em três gêneros: Lantana fucata, Lippia alba, Lippia corymbosa, Lippia diamantinensis, Lippia hederifolia, Lippia hermannioides, Lippia lacunosa, Lippia origanoides, Lippia pseudothea, Lippia raoniana, Lippia rotundifolia, Lippia stachyoides var. martiana, Stachytarpheta glabra e Stachytarpheta reticulata. Destacamos a ocorrência de Lippia raoniana, preliminarmente avaliada como criticamente ameaçada, dentro dos limites do Parque, expandindo o conhecimento sobre sua distribuição geográfica. Para reconher cada táxon, nós fornecemos descrições morfológicas, chave de identificação, fotografias, juntamente com comentários taxonômicos e ecológicos.

Orobanche dominae El Mokni (section Orobanche L.) is described and illustrated as a new species to science, endemic to central-eastern Tunisia, North Africa. The new species is known only from a single population within olive groves of Mahdia area, where it grows on sandy loam soil and parasiting on the Tuniso-Libyan endemic Onopordum espinae Bonnet. It is a medium almost brownish-purple plant characterized by a precocious and very short blooming period. It has a cylindrical, long inflorescence with erecto-patent and long sized flowers. The corolla exhibits varying colors along with short and long glandular hairs in addition to an almost glabrous style on the lower part. The macromorphological features suggest a closer relationship of this new species to the members of the subsections Minores Teryokhin and Speciosae Novopokr., in particular with Or. crenata Forssk., Or. minor Sm., and Or. artemisiae-campestris Gaudin s.l. (so far the only known Orobanche that was mentioned parasitizing one of the Onopordum species, On. illyricum L.). Relationships and discriminating morphological features of the new species with its closest species are examined. Data on its ecological and conservation status together with its main host(s) and distributive area are also presented.

RESUMO Nós apresentamos o tratamento taxonômico para a família Orobanchaceae da Serra de São José e da Serra do Lenheiro, no Estado de Minas Gerais, Brasil. Seis espécies e quatro gêneros foram registrados. Chave de identificação, descrições morfológicas, fotos e comentários sobre a distribuição geográfica, ecologia e taxonomia das espécies são apresentados.

Verbenaceae are a distinctive element from the Americas, and even when there are numerous useful species in the family until now they have not been listed. In line with this, we present the first checklist of Verbenaceae employed for food purposes in the continent. Seventy two bibliographical sources mentioning at least one edible Verbenaceae were selected from more than 500 references analyzed; as a result, 46 edible species have been registered. The family stands in the Americas regarding the use of aromatic plants. In fact, ca. 40% of the edible species are employed because of their aromatic traits, followed by edible fruits (ca. 33%). This collaborative effort represents a baseline for future research on this valuable lineage of flowering plants. [ABSTRACT FROM AUTHOR]

Acanthaceae is a family of tropical flowering plants with approximately 4900 species. Despite remarkable variation in morphological traits, research on patterns of character evolution has been limited by uncertain relationships among some of the major lineages. We sampled 16 taxa from these major lineages to estimate a phylogenomic framework using a combination of five newly sequenced shotgun genome skims plus seven new and four publicly available transcriptomes. We used OrthoFinder2 to infer a species tree with strong branch support. Except for the placement of Crabbea, our results corroborate the most recent chloroplast and nrITS sequence-based topology. Of 587 single copy loci, 10 were recovered for all 16 species; a RAxML tree estimated from these 10 loci resulted in the same topology as other datasets assembled in this study, with the exception of relationships among three sampled species of Barleria; however, branch support was lower compared to the tree reconstructed using more data. ABBA-BABA tests were conducted to investigate patterns of introgression involving Crabbea; few nucleotides supported alternative topologies. SplitsTree networks of the 587 loci and 6136 orthogroup trees revealed conflict among the branches leading to Andrographideae, Whitfieldieae, and Neuracanthus. A principal components analysis in treespace found no distinct clusters of trees. Our results based on combined genome skim and transcriptome sequences strongly corroborate the previously published chloroplast and nr-ITS-based phylogeny of Acanthaceae with increased resolution among Barlerieae, Andrographideae, Whitfieldieae, and Neuracanthus. This advance in our knowledge of Acanthaceae relationships will allow us to investigate character evolution and other phenomena within this diverse group of plants in studies with increased taxon sampling. [ABSTRACT FROM AUTHOR]

Mazus danxiacola, a new species endemic to Danxia landform in east Jiangxi Province, eastern China, is described and illustrated. The systematic placement of this new species was confirmed by molecular phylogenetic analyses based on four plastid markers (matK, rbcL, rps16 and trnL-trnF) and nuclear ribosome ITS sequence, and its specific relationships within Mazus were discussed. Morphologically, the new species is clearly different from other Mazus species by having a series of uncommon traits, i.e., annual habit, without stolons and basal leaves, single, erect and unbranched stems, long petiolate leaves abaxially grayish green to silver gray, truncate to broadly cuneate leaf bases, racemes extremely elongated up to 35 cm long, white corolla, and palate densely covered by conspicuous clavate gland-like hairs. The new species is assigned to Critically Endangered (CR) according to the IUCN Red List Categories and Criteria. [ABSTRACT FROM AUTHOR]

Premise: Phylogenomic datasets using genomes and transcriptomes provide rich opportunities beyond resolving bifurcating phylogenetic relationships. Monkeyflower (Phrymaceae) is a model system for evolutionary ecology. However, it lacks a well‐supported phylogeny as a basis for a stable taxonomy and for macroevolutionary comparisons. Methods: We sampled 24 genomes and transcriptomes in Phrymaceae and closely related families, including eight newly sequenced transcriptomes. We reconstructed the phylogeny using IQ‐TREE and ASTRAL, evaluated gene tree discordance using PhyParts, Quartet Sampling, and a cloudogram, and carried out reticulation analyses using PhyloNet and HyDe. We searched for whole genome duplication (WGD) events using chromosome numbers, synonymous distances, and gene duplication events as evidence. Results: Most gene trees support the monophyly of Phrymaceae and each of its tribes. Most gene trees also support tribe Mimuleae being sister to Phrymeae + Diplaceae + Leucocarpeae, with extensive gene tree discordance among the latter three. Despite the discordance, the monophyly of Mimulus s.l. is rejected, and no individual reticulation event among the Phrymaceae tribes is well‐supported. Reticulation likely occurred among Erythranthe bicolor and closely related species. No ancient WGD was detected in Phrymaceae. Instead, small‐scale duplications are among potential drivers of macroevolutionary diversification of Phrymaceae. Conclusions: We show that analysis of reticulate evolution is sensitive to taxon sampling and methods used. We also demonstrate that phylogenomic datasets using genomes and transcriptomes present rich opportunities to investigate gene family evolution and genome duplication events involved in lineage diversification and adaptation. [ABSTRACT FROM AUTHOR]

Background and aims – The last comprehensive study that estimated the number of Verbenaceae genera and species was published in 2004, and included 34 genera and around 1200 species. Since then, several publications based on morphology and/or molecular data have proposed important changes within the family. Due to the lack of updated literature to cite when referring to the number of Verbenaceae taxa, a review of these estimates is necessary.Key results and conclusion – We present a detailed list of genera currently accepted in Verbenaceae with the number of species contained in each and compare our numbers with the previous estimate. In addition, we indicate the geographic distribution and the most recent important taxonomic or phylogenetic works for each genus. Our compilation shows that Verbenaceae have 32 genera and 800 species currently accepted.This work provides up-to-date numbers and brings a holistic view of the family.