8 results on '"Lambilliotte, Raphaël"'
Search Results
2. Fungal Shaker-like channels beyond cellular K+ homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster
- Author
-
Garcia, Kevin, primary, Guerrero-Galán, Carmen, additional, Frank, Hannah E. R., additional, Haider, Muhammad Zulqurnain, additional, Delteil, Amandine, additional, Conéjéro, Geneviève, additional, Lambilliotte, Raphaël, additional, Fizames, Cécile, additional, Sentenac, Hervé, additional, and Zimmermann, Sabine D., additional
- Published
- 2020
- Full Text
- View/download PDF
3. Two differentially regulated phosphate transporters from the symbiotic fungus Hebeloma cylindrosporum and phosphorus acquisition by ectomycorrhizal Pinus pinaster
- Author
-
Tatry, Marie-Violaine, El Kassis, Elie, Lambilliotte, Raphaël, Corratgé, Claire, van Aarle, Ingrid, Amenc, Laurie K., Alary, Rémi, Zimmermann, Sabine, Sentenac, Hervé, and Plassard, Claude
- Published
- 2009
- Full Text
- View/download PDF
4. Fungal Shaker-like channels beyond cellular K+ homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster.
- Author
-
Garcia, Kevin, Guerrero-Galán, Carmen, Frank, Hannah E. R., Haider, Muhammad Zulqurnain, Delteil, Amandine, Conéjéro, Geneviève, Lambilliotte, Raphaël, Fizames, Cécile, Sentenac, Hervé, and Zimmermann, Sabine D.
- Subjects
CLUSTER pine ,CARRIER proteins ,BIOLOGICAL transport ,HOMEOSTASIS ,ION channels ,VOLTAGE-gated ion channels - Abstract
Potassium (K
+ ) acquisition, translocation and cellular homeostasis are mediated by various membrane transport systems in all organisms. We identified and described an ion channel in the ectomycorrhizal fungus Hebeloma cylindrosporum (HcSKC) that harbors features of animal voltage-dependent Shaker-like K+ channels, and investigated its role in both free-living hyphae and symbiotic conditions. RNAi lines affected in the expression of HcSKC were produced and used for in vitro mycorrhizal assays with the maritime pine as host plant, under standard or low K+ conditions. The adaptation of H. cylindrosporum to the downregulation of HcSKC was analyzed by qRT-PCR analyses for other K+ -related transport proteins: the transporters HcTrk1, HcTrk2, and HcHAK, and the ion channels HcTOK1, HcTOK2.1, and HcTOK2.2. Downregulated HcSKC transformants displayed greater K+ contents at standard K+ only. In such conditions, plants inoculated with these transgenic lines were impaired in K+ nutrition. Taken together, these results support the hypothesis that the reduced expression of HcSKC modifies the pool of fungal K+ available for the plant and/or affects its symbiotic transfer to the roots. Our study reveals that the maintenance of K+ transport in H. cylindrosporum, through the regulation of HcSKC expression, is required for the K+ nutrition of the host plant. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
5. Développement d'outils et de ressources moléculaires pour l'utilisation de l'espèce Hebeloma cylindrosporum comme modèle d'étude de la symbiose ectomycorhizienne
- Author
-
Lambilliotte, Raphaël, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de montpellier 2, Hervé Sentenac, and Sabine Zimmermann
- Subjects
Hebeloma cylindrosporum ,Gene duplication ,Canal potassique ,[SDV]Life Sciences [q-bio] ,Symbiose ,Transporteurs membranaires ,Ressource EST ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,cDNA library ,[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] ,Ectomycorrhiza ,Ectomycorhize ,Membrane transporters ,Banque d'ADNc ,EST database ,Shaker ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Potassium channel ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Symbiosis ,Duplication de gène - Abstract
Most plant species establish mycorrhizal symbiosis, which represents a major biological phenomenonin terrestrial ecosystems. Improvement of host plant mineral nutrition appears as an essential functionof this interaction. The fungal partner absorbs mineral nutrients from the soil and transfers part ofthem to the host plant. While membrane transport systems play thereby a key role in the symbioticinteraction, very few of them are characterised on a molecular level. The objective of this project is toidentify such transport systems in the ectomycorrhizal fungus Hebeloma cylindrosporum and then tocharacterise their function in the exchange of solutes with the plant host. Hebeloma cylindrosporumwill possibly become an interesting model as genetic analysis are feasible (e.g. the whole cycle, fromspore to spore, can be achieved in vitro). Our first goal was to produce a cDNA library, and then toestablish, by systematic sequencing and in silico analysis, an EST database (Expressed Sequence Tag).We identified approximately 2700 transcripts after in silico clustering. This database and its analysis isaccessible to the scientific community via a web site. Bioinformatic analysis revealed a large numberof putative transport systems in this database. We focalised our interest on one gene encoding a Shakerpotassium channel named HcSKC (for H. cylindrosporum Shaker K+ Channel). After screening of agenomic DNA library from Hebeloma h1 strain, several copies of this gene were identified andanalysed regarding their structure. We show that multicopy HcSKC sequences (that hamperedexperimental and bioinformatic analyses) are typical of h1 strain, whereas other Hebeloma strains andspecies possess a single copy. Based on sequence analysis, HcSKC code for an outward channel, thatmight thus be involved in fungal K+ secretion towards the host plant. This hypothesis will be verifiedby functional expression in heterologous systems.; La symbiose mycorhizienne concerne la majorité des espèces végétales et constitue un phénomènebiologique majeur dans les écosystèmes terrestres. Une de ses fonctions essentielles est l'améliorationde la nutrition minérale de la plante hôte. Le partenaire fongique prélève des nutriments minéraux dansle sol et les transmet à la plante. Bien qu'ils soient au cœur de l'interaction symbiotique, lesmécanismes de transport membranaire responsables de ces échanges sont encore peu caractérisés surle plan moléculaire. Le programme dans lequel s'inscrit notre travail a pour objectif d'identifier de telstransporteurs chez le champignon ectomycorhizien Hebeloma cylindrosporum, puis de préciser lafonction que ces systèmes jouent dans les échanges de solutés avec la plante hôte. H. cylindrosporumest susceptible de devenir un modèle très intéressant parce qu'il se prête aux analyses génétiques, avecun cycle biologique entièrement maîtrisé in vitro. Notre premier objectif a été de produire une banqued'ADNc puis, par séquençage systématique et analyse in silico, une ressource d'EST ("ExpressedSequenced Tags"). Nous avons ainsi identifié environ 2700 transcrits (après assemblage in silico desséquences), et mis cette ressource ainsi que son analyse bioinformatique à la disposition de lacommunauté scientifique sur un site web. L'analyse bioinformatique a révélé un grand nombre desystèmes de transport membranaire potentiels au sein de cette ressource. Nous nous sommes intéressésen particulier à un gène codant un canal potassique de type Shaker, que nous avons appelé HcSKC(pour H. cylindrosporum Shaker K+ Channel). Après criblage d'une banque d'ADN génomiqueprovenant de la souche h1 d'Hebeloma, nous avons identifié plusieurs copies de ce gène et analysé leurstructure. Nous montrons que la présence de plusieurs copies d'HcSKC (qui accroît la difficulté desanalyses expérimentales et bioinformatiques) est une caractéristique de la souche h1 puisque d'autressouches ainsi que d'autres espèces d'hébélomes ne présentent qu'une seule copie de ce gène. Sur labase de sa séquence, le canal codé par HcSKC est susceptible de jouer un rôle dans la sécrétion de K+par le champignon en direction de la plante. Cette hypothèse devra être vérifiée par expressionfonctionnelle en système hétérologue.
- Published
- 2004
6. Molecular and Functional Characterization of a Na+-K+ Transporter from the Trk Family in the Ectomycorrhizal Fungus Hebeloma cylindrosporum
- Author
-
Corratgé, Claire, primary, Zimmermann, Sabine, additional, Lambilliotte, Raphaël, additional, Plassard, Claude, additional, Marmeisse, Roland, additional, Thibaud, Jean-Baptiste, additional, Lacombe, Benoît, additional, and Sentenac, Hervé, additional
- Published
- 2007
- Full Text
- View/download PDF
7. Large-scale identification of genes in the fungusHebeloma cylindrosporumpaves the way to molecular analyses of ectomycorrhizal symbiosis.
- Author
-
Lambilliotte, Raphaël, Cooke, Richard, Samson, Delphine, Fizames, Cécile, Gaymard, Frédéric, Plassard, Claude, Tatry, Marie-Violaine, Berger, Christel, Laudié, Michèle, Legeai, Fabrice, Karsenty, Emmanuelle, Delseny, Michel, Zimmermann, Sabine, and Sentenac, Hervé
- Subjects
- *
GENES , *ECTOMYCORRHIZAL fungi , *SYMBIOSIS , *PLANT growth , *MOLECULAR genetics - Abstract
• In an attempt to advance the understanding of ectomycorrhizal symbiosis, which improves mineral nutrition and resistance to abiotic and biotic stresses of most woody plants from temperate and boreal regions, we produced a cDNA library of the ectomycorrhizal fungusHebeloma cylindrosporumin a yeast expression vector. The library was used for functional complementation cloning and for sequencing of 4036 expressed sequence tags (ESTs).• Expression of the library in a yeastade2andtrp1mutant strain allowed cloning of twoHebelomagenes,HcADE2andHcTRP1by functional complementation. This experiment highlighted both the efficiency of the complementation strategy and the quality of the library.• All sequence data available onHebeloma(4596) were analysed and contiged. The whole EST resource is accessibleviaa web interface, which allows searching and navigation through clusters and tentative contigs (TCs).• Analysis of the database revealed a low level of counterparts already identified in other ectomycorrhizal fungi. For example, it allowed identification of a large set of membrane transporters, such as phosphate, potassium, sulphate or micronutrient transporters. Thus, this EST resource provides a valuable tool for research on ectomycorrhiza and symbiotic interactions.New Phytologist(2004)doi: 10.1111/j.1469-8137.2004.01185.x© New Phytologist(2004) [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
8. Molecular and Functional Characterization of a Na+-K+ Transporter from the Trk Family in the Ectomycorrhizal Fungus Hebeloma cylindrosporum.
- Author
-
Corratgé, Claire, Zimmermann, Sabine, Lambilliotte, Raphaël, Plassard, Claude, Marmeisse, Roland, Thibaud, Jean-Baptiste, Lacombe, Benoit, and Sentenac, Hervé
- Subjects
- *
ECTOMYCORRHIZAS , *ECTOMYCORRHIZAL fungi , *HEBELOMA , *SACCHAROMYCES cerevisiae , *MYCORRHIZAL fungi , *SYMBIOSIS - Abstract
Ectomycorrhizal symbiosis between fungi and woody plants strongly improves plant mineral nutrition and constitutes a major biological process in natural ecosystems. Molecular identification and functional characterization of fungal transport systems involved in nutrient uptake are crucial steps toward understanding the improvement of plant nutrition and the symbiotic relationship itself. In the present report a transporter belonging to the Trk family is identified in the model ectomycorrhizal fungus Hebeloma cylindrosporum and named HcTrk1. The Trk family is still poorly characterized, although it plays crucial roles in K+ transport in yeasts and filamentous fungi. In Saccharomyces cerevisiae K+ uptake is mainly dependent on the activity of Trk transporters thought to mediate H+:K+ symport. The ectomycorrhizal HcTrk1 transporter was functional when expressed in Xenopus oocytes, enabling the first electrophysiological characterization of a transporter from the Trk family. HcTrk1 mediates instantaneously activating inwardly rectifying currents, is permeable to both K+ and Na+, and displays channel-like functional properties. The whole set of data and particularly a phenomenon reminiscent of the anomalous mole fraction effect suggest that the transport does not occur according to the classical alternating access model. Permeation appears to occur through a single-file pore, where interactions between Na+ and K+ might result in Na+:K+ co-transport activity. HcTrk1 is expressed in external hyphae that explore the soil when the fungus grows in symbiotic condition. Thus, it could play a major role in both the K+ and Na+ nutrition of the fungus (and of the plant) in nutrient-poor soils. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.