36 results on '"Lai, Sheng-Feng"'
Search Results
2. Gold nanoparticles: BSA (Bovine Serum Albumin) coating and X-ray irradiation produce variable-spectrum photoluminescence
- Author
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Lee, Kuo-Hao, Lai, Sheng-Feng, Lin, Yan-Cheng, Chou, Wu-Ching, Ong, Edwin B.L., Tan, Hui-Ru, Tok, Eng Soon, Yang, C.S., Margaritondo, G., and Hwu, Y.
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- 2015
- Full Text
- View/download PDF
3. The Associations Between Psychological Distress and Academic Burnout: A Mediation and Moderation Analysis
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Chen, Hui Ling, primary, Wang, Hui Yuan, additional, Lai, Sheng Feng, additional, and Ye, Zeng Jie, additional
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- 2022
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4. Very small photoluminescent gold nanoparticles for multimodality biomedical imaging
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Lai, Sheng-Feng, Chien, Chia-Chi, Chen, Wen-Chang, Chen, Hsiang-Hsin, Chen, Yi-Yun, Wang, Cheng-Liang, Hwu, Y., Yang, C.S., Chen, C.Y., Liang, K.S., Petibois, Cyril, Tan, Hui-Ru, Tok, Eng-Soon, and Margaritondo, G.
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- 2013
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5. Imaging the cellular uptake of tiopronin-modified gold nanoparticles
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Cai, Xiaoqing, Chen, Hsiang-Hsin, Wang, Cheng-Liang, Chen, Shin-Tai, Lai, Sheng-Feng, Chien, Chia-Chi, Chen, Yi-Yun, Kempson, Ivan M., Hwu, Yeukuang, Yang, C. S., and Margaritondo, G.
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- 2011
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6. Gold nanoparticles as high-resolution X-ray imaging contrast agents for the analysis of tumor-related micro-vasculature
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Chien Chia-Chi, Chen Hsiang-Hsin, Lai Sheng-Feng, Wu Kang-Chao, Cai Xiaoqing, Hwu Yeukuang, Petibois Cyril, Chu Yong, and Margaritondo Giorgio
- Subjects
Synchrotron ,X-rays ,Angiography ,Angiogenesis ,Contrast ,Au Nanoparticles ,Heparin ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Background Angiogenesis is widely investigated in conjunction with cancer development, in particular because of the possibility of early stage detection and of new therapeutic strategies. However, such studies are negatively affected by the limitations of imaging techniques in the detection of microscopic blood vessels (diameter 3-5 μm) grown under angiogenic stress. We report that synchrotron-based X-ray imaging techniques with very high spatial resolution can overcome this obstacle, provided that suitable contrast agents are used. Results We tested different contrast agents based on gold nanoparticles (AuNPs) for the detection of cancer-related angiogenesis by synchrotron microradiology, microtomography and high resolution X-ray microscopy. Among them only bare-AuNPs in conjunction with heparin injection provided sufficient contrast to allow in vivo detection of small capillary species (the smallest measured lumen diameters were 3-5 μm). The detected vessel density was 3-7 times higher than with other nanoparticles. We also found that bare-AuNPs with heparin allows detecting symptoms of local extravascular nanoparticle diffusion in tumor areas where capillary leakage appeared. Conclusions Although high-Z AuNPs are natural candidates as radiology contrast agents, their success is not guaranteed, in particular when targeting very small blood vessels in tumor-related angiography. We found that AuNPs injected with heparin produced the contrast level needed to reveal--for the first time by X-ray imaging--tumor microvessels with 3-5 μm diameter as well as extravascular diffusion due to basal membrane defenestration. These results open the interesting possibility of functional imaging of the tumor microvasculature, of its development and organization, as well as of the effects of anti-angiogenic drugs.
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- 2012
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7. Gold nano-mesh synthesis by continuous-flow X-ray irradiation
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Li, Min-Tsang, primary, Lai, Sheng-Feng, additional, Yang, Shun-Min, additional, Chen, Yu-Sheng, additional, Chen, Ying-Jie, additional, Tok, Eng Soon, additional, Margaritondo, Giorgio, additional, and Hwu, Yeukuang, additional
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- 2019
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8. Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy
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Hua Tzu-En, Lai Sheng-Feng, Chu Yong S, Wang Cheng-Liang, Petibois Cyril, Chien Chia-Chi, Chen Hsiang-Hsin, Chen Yi-Yun, Cai Xiaoqing, Kempson Ivan M, Hwu Yeukuang, and Margaritondo Giorgio
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Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Background Quantitative analysis of nanoparticle uptake at the cellular level is critical to nanomedicine procedures. In particular, it is required for a realistic evaluation of their effects. Unfortunately, quantitative measurements of nanoparticle uptake still pose a formidable technical challenge. We present here a method to tackle this problem and analyze the number of metal nanoparticles present in different types of cells. The method relies on high-lateral-resolution (better than 30 nm) transmission x-ray microimages with both absorption contrast and phase contrast -- including two-dimensional (2D) projection images and three-dimensional (3D) tomographic reconstructions that directly show the nanoparticles. Results Practical tests were successfully conducted on bare and polyethylene glycol (PEG) coated gold nanoparticles obtained by x-ray irradiation. Using two different cell lines, EMT and HeLa, we obtained the number of nanoparticle clusters uptaken by each cell and the cluster size. Furthermore, the analysis revealed interesting differences between 2D and 3D cultured cells as well as between 2D and 3D data for the same 3D specimen. Conclusions We demonstrated the feasibility and effectiveness of our method, proving that it is accurate enough to measure the nanoparticle uptake differences between cells as well as the sizes of the formed nanoparticle clusters. The differences between 2D and 3D cultures and 2D and 3D images stress the importance of the 3D analysis which is made possible by our approach.
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- 2011
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9. Gold nanoparticles as multimodality imaging agents for brain gliomas
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Lai, Sheng-Feng, primary, Ko, Bai-Hung, additional, Chien, Chia-Chi, additional, Chang, Chia-Ju, additional, Yang, Shun-Ming, additional, Chen, Hsiang-Hsin, additional, Petibois, Cyril, additional, Hueng, Dueng-Yuan, additional, Ka, Shuk-Man, additional, Chen, Ann, additional, Margaritondo, G., additional, and Hwu, Y., additional
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- 2015
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- View/download PDF
10. X-ray irradiation synthesis of PEG-coated Au-Pd nanoparticles
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Li, Min-Tsang, primary, Wang, Chang-Hai, additional, Lai, Sheng-Feng, additional, Ong, Edwin B L, additional, Chen, Y H, additional, Lin, Chung-Kwei, additional, Margaritondo, G, additional, and Hwu, Y, additional
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- 2015
- Full Text
- View/download PDF
11. Optimization of gold nanoparticle photoluminescence by alkanethiolation
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Lai, Sheng-Feng, primary, Tan, Hui-Ru, additional, Tok, Eng Soon, additional, Chen, Yu-Han, additional, Ong, Edwin B. L., additional, Li, Min-Tsang, additional, Chen, Yi-Yun, additional, Chien, Fan-Ching, additional, Chen, Peilin, additional, Margaritondo, G., additional, and Hwu, Y., additional
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- 2015
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- View/download PDF
12. Uncapped Au–Pd colloidal nanoparticles show catalytic enhancement
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Li, Min-Tsang, primary, Wang, Chang-Hai, additional, Lai, Sheng-Feng, additional, Chen, Yu-Han, additional, Ong, Edwin B. L., additional, Lin, Chung-Kwei, additional, Margaritondo, G., additional, and Hwu, Y., additional
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- 2015
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13. X-ray imaging of tumor growth in live mice by detecting gold-nanoparticle-loaded cells
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Chien, Chia-Chi, primary, Chen, Hsiang-Hsin, additional, Lai, Sheng-Feng, additional, Hwu, Y., additional, Petibois, Cyril, additional, Yang, C. S., additional, Chu, Y., additional, and Margaritondo, G., additional
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- 2012
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14. Size control of gold nanoparticles by intense X-ray irradiation: the relevant parameters and imaging applications
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Lai, Sheng-Feng, primary, Chien, Chia-Chi, additional, Chen, Wen-Chang, additional, Chen, Yi-Yun, additional, Wang, Chang-Hai, additional, Hwu, Y., additional, Yang, C. S., additional, and Margaritondo, G., additional
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- 2012
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15. One-Pot Tuning of Au Nucleation and Growth: From Nanoclusters to Nanoparticles
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Lai, Sheng-Feng, primary, Chen, Wen-Chang, additional, Wang, Cheng-Liang, additional, Chen, Hsiang-Hsin, additional, Chen, Shin-Tai, additional, Chien, Chia-Chi, additional, Chen, Yi-Yun, additional, Hung, Wen-Ting, additional, Cai, Xiaoqing, additional, Li, Enrong, additional, Kempson, Ivan M., additional, Hwu, Y., additional, Yang, C. S., additional, Tok, Eng-Soon, additional, Tan, Hui Ru, additional, Lin, Ming, additional, and Margaritondo, G., additional
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- 2011
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16. Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy
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Chen, Hsiang-Hsin, primary, Chien, Chia-Chi, additional, Petibois, Cyril, additional, Wang, Cheng-Liang, additional, Chu, Yong S, additional, Lai, Sheng-Feng, additional, Hua, Tzu-En, additional, Chen, Yi-Yun, additional, Cai, Xiaoqing, additional, Kempson, Ivan M, additional, Hwu, Yeukuang, additional, and Margaritondo, Giorgio, additional
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- 2011
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- View/download PDF
17. One-pot synthesis of AuPt alloyed nanoparticles by intense x-ray irradiation
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Wang, Cheng-Liang, primary, Hsao, Bo-Jun, additional, Lai, Sheng-Feng, additional, Chen, Wen-Chang, additional, Chen, Hsiang-Hsin, additional, Chen, Yi-Yun, additional, Chien, Chia-Chi, additional, Cai, Xiaoqing, additional, Kempson, Ivan M, additional, Hwu, Y, additional, and Margaritondo, G, additional
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- 2011
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18. Tailored Au nanorods: optimizing functionality, controlling the aspect ratio and increasing biocompatibility
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Cai, Xiaoqing, primary, Wang, Cheng-Liang, additional, Chen, Hsiang-Hsin, additional, Chien, Chia-Chi, additional, Lai, Sheng-Feng, additional, Chen, Yi-Yun, additional, Hua, Tzu-En, additional, Kempson, Ivan M, additional, Hwu, Y, additional, Yang, C S, additional, and Margaritondo, G, additional
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- 2010
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19. Enhancement of irradiation effects on cancer cells by cross-linked dextran-coated iron oxide (CLIO) nanoparticles
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Huang, Fu-Kuo, primary, Chen, Wen-Chang, additional, Lai, Sheng-Feng, additional, Liu, Chi-Jen, additional, Wang, Cheng-Liang, additional, Wang, Chang-Hai, additional, Chen, Hsiang-Hsin, additional, Hua, Tzu-En, additional, Cheng, Yi-Yun, additional, Wu, M K, additional, Hwu, Y, additional, Yang, Chung-Shi, additional, and Margaritondo, G, additional
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- 2009
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20. Imaging the cellular uptake of tiopronin-modified gold nanoparticles
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Cai, Xiaoqing, Chen, Hsiang-Hsin, Wang, Cheng-Liang, Chen, Shin-Tai, Lai, Sheng-Feng, Chien, Chia-Chi, Chen, Yi-Yun, Kempson, Ivan M., Hwu, Yeukuang, Yang, C. S., and Margaritondo, G.
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Microscopy ,Cytotoxicity ,X-Ray-Irradiation ,CIBM-PC ,Catalysis ,Different Sizes ,Mammalian-Cells ,Complexes ,Cellular uptake ,Gold nanoparticles ,Au Clusters ,Resolution ,Transmission X-ray microscopy ,X-ray synthesis ,Tomography - Abstract
Well-dispersed gold nanoparticles (NP) coated with tiopronin were synthesized by X-ray irradiation without reducing agents. High-resolution transmission electron microscopy shows that the average core diameters of the NPs can be systematically controlled by adjusting the tiopronin to Au mole ratio in the reaction. Three methods were used to study the NP uptake by cells: quantitative measurements by inductively coupled plasma mass spectrometry, direct imaging with high lateral resolution transmission electron microscopy and transmission X-ray microscopy. The results confirmed that the NP internalization mostly occurred via endocytosis and concerned the cytoplasm. The particles, in spite of their small sizes, were not found to arrive inside the cell nuclei. The synthesis without reducing agents and solvents increased the biocompatibility as required for potential applications in analysis and biomedicine in general.
21. One-Pot Tuning of Au Nucleation and Growth: From Nanoclusters to Nanoparticles
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Lai, Sheng-Feng, Chen, Wen-Chang, Wang, Cheng-Liang, Chen, Hsiang-Hsin, Chen, Shin-Tai, Chien, Chia-Chi, Chen, Yi-Yun, Hung, Wen-Ting, Cai, Xiaoqing, Li, Enrong, Kempson, Ivan M., Hwu, Y., Yang, C. S., Tok, Eng-Soon, Tan, Hui Ru, Lin, Ming, and Margaritondo, G.
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Size Distribution ,Sensitivity ,genetic structures ,X-Ray-Irradiation ,Gold Nanoparticles ,Shape ,Biology ,CIBM-PC ,Reduction ,Model - Abstract
We describe a simple and effective method to obtain colloidal surface-functionalized Au nanoparticles. The method is primarily based on irradiation of a gold solution with high-flux X-rays from a synchrotron source in the presence of 11-mercaptoundecanoic acid (MUA). Extensive tests of the products demonstrated high colloidal density as well as excellent stability, shelf life, and biocompatibility. Specific tests with X-ray diffraction, UV-visible spectrometry, visible microscopy, Fourier transform infrared spectroscopy, dark-field visible-light scattering microscopy, and transmission electron microscopy demonstrated that MUA, being an effective surfactant, not only allows tunable size control of the nanoparticles, but also facilitates functionalization. The nanoparticle sizes were 6.45 +/- 1.58, 1.83 +/- 1.21, 1.52 +/- 0.37 and 1.18 +/- 0.26 nm with no MUA and with MUA-to-Au ratios of 1:2, 1:1, and 3:1. The MUA additionally enabled functionalization with L-glycine. We thus demonstrated flexibility in controlling the nanoparticle size over a large range with narrow size distribution.
22. Tailored Au nanorods: optimizing functionality, controlling the aspect ratio and increasing biocompatibility
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Cai, Xiaoqing, Wang, Cheng-Liang, Chen, Hsiang-Hsin, Chien, Chia-Chi, Lai, Sheng-Feng, Chen, Yi-Yun, Hua, Tzu-En, Kempson, Ivan M., Hwu, Y., Yang, C. S., and Margaritondo, G.
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Gold Nanorods ,Cells ,X-Ray-Irradiation ,Optical-Properties ,Growth ,Therapy ,Shape-Controlled Synthesis ,Composite Nanoparticles ,Tomography ,Electron ,CIBM-PC - Abstract
Monodisperse gold nanorods with high aspect ratio were synthesized by x-ray irradiation. Irradiation was first used to stimulate the creation of seeds. Afterward, nanorod growth was stimulated either by chemical reduction or again by x-ray irradiation. In the last case, the entire process took place without reducing agents. The shape of the final products could be controlled by modulating the intensity of the x-ray irradiation during the seed synthesis. In turn, the nanorod aspect ratio determines the absorption wavelength of the nanorods that can thus be optimized for different applications. Likewise, the aspect ratio influences the uptake of the nanorods by HeLa cells.
23. Very small photoluminescent gold nanoparticles for multimodality biomedical imaging
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Lai, Sheng-Feng, Chien, Chia-Chi, Chen, Wen-Chang, Chen, Hsiang-Hsin, Chen, Yi-Yun, Wang, Cheng-Liang, Hwu, Y., Yang, C. S., Chen, C. Y., Liang, K. S., Petibois, Cyril, Tan, Hui-Ru, Tok, Eng-Soon, and Margaritondo, G.
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CIBM-PC - Abstract
An original synthesis method based on X-ray irradiation produced gold nanoparticles (AuNPs) with two important properties for biomedical research: intense visible photoluminescence and very high accumulation in cancer cells. The nanoparticles, coated with MUA (11-mercaptoundecanoid acid), are very small (1.4 nm diameter); the above two properties are not present for even slightly larger sizes. The small MUA-AuNPs are non-cytotoxic (except for very high concentrations) and do not interfere with cancer cell proliferation. Multimodality imaging using visible light fluorescence and X-ray microscopy is demonstrated by tracing the nanoparticle-loaded tumor cells. (C) 2012 Elsevier Inc. All rights reserved.
24. One-pot synthesis of AuPt alloyed nanoparticles by intense x-ray irradiation
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Wang, Cheng-Liang, Hsao, Bo-Jun, Lai, Sheng-Feng, Chen, Wen-Chang, Chen, Hsiang-Hsin, Chen, Yi-Yun, Chien, Chia-Chi, Cai, Xiaoqing, Kempson, Ivan M., Hwu, Y., and Margaritondo, G.
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Simulations ,Clusters ,Catalysts ,technology, industry, and agriculture ,Gold Nanoparticles ,macromolecular substances ,equipment and supplies ,CIBM-PC ,Bimetallic Nanoparticles - Abstract
We synthesized AuPt alloyed nanoparticles in colloidal solution by a one-pot procedure based on synchrotron x-ray irradiation in the presence of PEG (polyethylene glycol). The exclusive presence of alloyed nanoparticles with fcc structure was confirmed by several different experiments including UV-vis spectroscopy, x-ray diffraction (XRD) and transmission electron microscopy (TEM). The composition of the AuPt alloyed nanoparticles can be varied in a continuous fashion by simply varying the feed ratios of Au and Pt precursors. The nanoparticles exhibited colloidal stability and biocompatibility, important for potential applications.
25. Gold nanoparticles: BSA (Bovine Serum Albumin) coating and X-ray irradiation produce variable-spectrum photoluminescence
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Lee, Kuo-Hao, Lai, Sheng-Feng, Lin, Yan-Cheng, Chou, Wu-Ching, Ong, Edwin B. L., Tan, Hui-Ru, Tok, Eng Soon, Yang, C. S., Margaritondo, G., and Hwu, Y.
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Luminescence ,Visible and ultraviolet spectrometers ,sense organs ,Nanostructures ,Irradiation effect - Abstract
We show that by using different x-ray irradiation times of BSA-coated Au nanoparticles (NPs) we can change their ultraviolet-stimulated photoluminescence and shift the spectral weight over the visible spectral range. This is due to the interplay of two emission bands, one due to BSA and the other related to gold. The emission properties did not change with time over a period of several months. (C) 2014 Published by Elsevier B.V.
26. Size control of gold nanoparticles by intense X-ray irradiation: the relevant parameters and imaging applications
- Author
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Lai, Sheng-Feng, Chien, Chia-Chi, Chen, Wen-Chang, Chen, Yi-Yun, Wang, Chang-Hai, Hwu, Y., Yang, C. S., and Margaritondo, G.
- Subjects
Cellular Uptake ,Microscopy ,Accumulation ,Enhancement ,Cells ,Therapeutics ,Mechanism ,Resolution ,CIBM-PC ,Particle-Size ,Reduction - Abstract
We report the analysis of a reducing-agent-free synthetic method based on intense X-ray irradiation in the presence of 11-mercaptoundecanoic acid (MUA) and their application in biomedical imaging. The method is very effective in controlling the size and size distribution of Au nanoparticles in colloidal solutions: gold nanoparticles (AuNPs) as small as similar to 1.2 +/- 0.25 nm are synthesized in a one-pot irradiation procedure without reducing agents. We found two coexisting properties of these small AuNPs: a strong visible fluorescence and very high accumulation in cells, which enabled a new type of multimodality imaging using X-ray and visible light microscopies. These properties come as a direct consequence of the precise size control; specifically, they exist only when the particle size is smaller than similar to 1.4 nm. We found that this process critically depends on several parameters including the solution pH, the solution temperature and the irradiation time. Most important is the fact that the increase of the irradiation time is not necessarily beneficial, since an optimum value exists to obtain small particles with a narrow size distribution. The 1.4 nm AuNPs are not toxic and significantly accumulate in tumor tissues in animal tests, as confirmed by X-ray imaging and fluorescence microscopy. This biodistribution could open the way for the use of these nanoparticles in X-ray imaging.
27. Optimization of Gold Nanoparticle Photoluminescence by Alkanethiolation
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Lai, Sheng-Feng, Tan, Hui Ru, Tok, Eng Soon, Chen, Yu-Han, Ong, Edwin B. L., Li, Min-Tsang, Chen, Yi-Yun, Chien, Fan-Ching, Chen, Peilin, Margaritondo, Giorgio, and Hwu, Yeukuang
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CIBM-PC - Abstract
Surface thiolation was recently found to affect two important properties of gold nanoparticles: their size and the presence of visible luminescence under UV stimulation. We explored these phenomena by systematically analysing alkanethiolate coatings with different carbon chain lengths, from 3-mercaptopropionic acid to 16-mercaptohexadecanoic acid. In all cases, the coating was achieved by rapid reduction of metal ions stimulated by intense x-ray irradiation. For small surfactant-to-metal ratios in the solution, the nanoparticle size monotonically decreases with the length. Photoluminescence is present for the smallest nanoparticles, but its intensity becomes more intense as the carbon chain length increases. With 16-mercaptohexadecanoic acid coating, the nanoparticles achieved a 28% quantum efficiency which make them very bright fluorophores with a large Stokes shift, both strongly desired performances for biomedical imaging. These particles also yield strong luminescence with multiphoton excitation which could find imaging application with standard experimental setups. Besides their fundamental interest in clarifying the mechanism for luminescence, these results could find interesting practical applications in nanoparticle production and in the use of fluorescence for imaging in biology and other areas.
28. X-ray irradiation synthesis of PEG-coated Au-Pd nanoparticles
- Author
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Li, Min-Tsang, Wang, Chang-Hai, Lai, Sheng-Feng, Ong, Edwin B. L., Chen, Y. H., Lin, Chung-Kwei, Margaritondo, G., and Hwu, Y.
- Subjects
catalytic property ,alloy nanoparticle ,core-shell structure ,Au-Pd ,PEG ,CIBM-PC - Abstract
We demonstrate that the combination of x-ray irradiation and capping by polyethylene glycol (PEG) produces excellent flexibility in controlling the structure of Au-Pd nanoparticles while preserving their catalytic performance. We specifically adopted two different fabrication methods: co-reduction and seed-assisted reduction. In both cases, precursor composition plays an important role in controlling the phases and size of the bimetallic nanoparticles. The optimal catalytic performance is obtained with the highest Pd concentration and when the nanoparticles consist of a Au core and a Pd shell.
29. Uncapped Au-Pd colloidal nanoparticles show catalysis enhancement
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Li, Min-Tsang, Wang, Changhai, Lai, Sheng-Feng, Chen, Yu-Han, Ong, Edwin B. L., Lin, Chung-Kwei, Margaritondo, Giorgio, and Hwu, Yeukuang
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CIBM-PC - Abstract
The catalytic properties of Pd trigger a strong interest on the related catalysis by Au-Pd nanoparticles. However, the analysis of such phenomena was complicated so far by the presence of capping. Using x-ray irradiation, we could produce uncapped Au-Pd nanoparticles and study their catalytic features, finding in particular their relation to the Pd content. Furthermore, the fabrication process is per se interesting, yielding excellent and flexibly controllable nanoparticles with a rather simple procedure.
30. One-Pot Tuning of Au Nucleation and Growth: From Nanoclusters to Nanoparticles
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Ivan M. Kempson, Eng Soon Tok, S.-Y. Chen, Yi-Yun Chen, Giorgio Margaritondo, Sheng-Feng Lai, Cheng-Liang Wang, Wen-Chang Chen, Chung-Shi Yang, Ming Lin, Wen-Ting Hung, Enrong Li, Chia-Chi Chien, Xiaoqing Cai, Yeukuang Hwu, Hui Ru Tan, Hsiang-Hsin Chen, Lai, Sheng-Feng, Chen, Wen-Chang, Wang, Cheng-Liang, Chen, Hsiang-Hsin, Chen, Shin-Tai, Chien, Chia-Chi, Chen, Yi-Yun, Hung, Wen-Ting, Cai, Xiaoqing, Li, Enrong, Kempson, Ivan M, Hwu, Y, Yang, CS, Tok, Eng-Soon, Tan, Hui Ru, Lin, Ming, and Margaritondo, G
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genetic structures ,Cell Survival ,Surface Properties ,Analytical chemistry ,Nucleation ,Metal Nanoparticles ,Nanoparticle ,Infrared spectroscopy ,Biocompatible Materials ,Nanoclusters ,Mice ,Structure-Activity Relationship ,Cell Line, Tumor ,size distribution ,Microscopy ,Electrochemistry ,Animals ,General Materials Science ,Colloids ,Sulfhydryl Compounds ,Particle Size ,Fourier transform infrared spectroscopy ,Spectroscopy ,irradiation ,Dose-Response Relationship, Drug ,Chemistry ,Fatty Acids ,Surfaces and Interfaces ,Condensed Matter Physics ,Transmission electron microscopy ,Colloidal gold ,Gold ,Au nanoparticles - Abstract
We describe a simple and effective method to obtain colloidal surface-functionalized Au nanoparticles. The method is primarily based on irradiation of a gold solution with high-flux X-rays from a synchrotron source in the presence of 11-mercaptoundecanoic acid (MUA). Extensive tests of the products demonstrated high colloidal density as well as excellent stability, shelf life, and biocompatibility. Specific tests with X-ray diffraction, UV-visible spectrometry, visible microscopy, Fourier transform infrared spectroscopy, dark-field visible-light scattering microscopy, and transmission electron microscopy demonstrated that MUA, being an effective surfactant, not only allows tunable size control of the nanoparticles, but also facilitates functionalization. The nanoparticle sizes were 6.45 ± 1.58, 1.83 ± 1.21, 1.52 ± 0.37 and 1.18 ± 0.26 nm with no MUA and with MUA-to-Au ratios of 1:2, 1:1, and 3:1. The MUA additionally enabled functionalization with l-glycine. We thus demonstrated flexibility in controlling the nanoparticle size over a large range with narrow size distribution. Refereed/Peer-reviewed
- Published
- 2011
31. Imaging the cellular uptake of tiopronin-modified gold nanoparticles
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Xiaoqing Cai, S.-Y. Chen, Hsiang-Hsin Chen, Yeukuang Hwu, Ivan M. Kempson, Yi-Yun Chen, Giorgio Margaritondo, Sheng-Feng Lai, Cheng-Liang Wang, Chia-Chi Chien, Chung-Shi Yang, Cai, Xiaoqing, Chen, Hsiang-Hsin, Wang, Cheng-Liang, Chen, Shin-Tai, Lai, Sheng-Feng, Chien, Chia-Chi, Chen, Yi-Yun, Kempson, Ivan M, Hwu, Yeukuang, Yang, C S, and Margaritondo, G
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Resolution (mass spectrometry) ,Biocompatibility ,Cell Survival ,Reducing agent ,Cells ,Analytical chemistry ,Metal Nanoparticles ,Biochemistry ,Mass Spectrometry ,Analytical Chemistry ,Microscopy, Electron, Transmission ,Microscopy ,medicine ,Humans ,Inductively coupled plasma mass spectrometry ,Cells, Cultured ,Chemistry ,X-Rays ,Tiopronin ,Endocytosis ,Radiography ,Transmission electron microscopy ,Colloidal gold ,Gold ,Synchrotrons ,medicine.drug ,Nuclear chemistry - Abstract
Well-dispersed gold nanoparticles (NP) coated with tiopronin were synthesized by X-ray irradiation without reducing agents. High-resolution transmission electron microscopy shows that the average core diameters of the NPs can be systematically controlled by adjusting the tiopronin to Au mole ratio in the reaction. Three methods were used to study the NP uptake by cells: quantitative measurements by inductively coupled plasma mass spectrometry, direct imaging with high lateral resolution transmission electron microscopy and transmission X-ray microscopy. The results confirmed that the NP internalization mostly occurred via endocytosis and concerned the cytoplasm. The particles, in spite of their small sizes, were not found to arrive inside the cell nuclei. The synthesis without reducing agents and solvents increased the biocompatibility as required for potential applications in analysis and biomedicine in general Refereed/Peer-reviewed
- Published
- 2011
32. Gold nanoparticles as multimodality imaging agents for brain gliomas
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Giorgio Margaritondo, Chia-Chi Chien, Hsiang Hsin Chen, Ann Chen, Sheng Feng Lai, Shun‑Ming Yang, Cyril Petibois, Dueng-Yuan Hueng, Bai‑Hung Ko, Shuk-Man Ka, Chia Ju Chang, Yeukuang Hwu, Lai, Sheng-Feng, Ko, Bai-Hung, Chien, Chia-Chi, Chang, Chia-Ju, Yang, Shun-Ming, Chen, Hsiang-Hsin, Petibois, Cyril, Hueng, Dueng-Yuan, Ka, Shuk-Man, Chen, Ann, Margaritondo, G, and Hwu, Y
- Subjects
fiber optic sensors ,Fluorescence-lifetime imaging microscopy ,Pharmaceutical Science ,Medicine (miscellaneous) ,Nanoparticle ,Contrast Media ,Metal Nanoparticles ,02 engineering and technology ,Applied Microbiology and Biotechnology ,Mice ,Microscopy ,Medicine ,metal nanoparticles ,Internalization ,media_common ,0303 health sciences ,Brain Neoplasms ,Optical Imaging ,Brain ,Glioma ,021001 nanoscience & nanotechnology ,Endocytosis ,3. Good health ,Colloidal gold ,Molecular Medicine ,Science & Technology - Other Topics ,fluorescence ,0210 nano-technology ,media_common.quotation_subject ,Biomedical Engineering ,Bioengineering ,Nanotechnology ,03 medical and health sciences ,In vivo ,Animals ,Nanoscience & Nanotechnology ,030304 developmental biology ,business.industry ,Research ,gold ,medicine.disease ,imaging techniques ,Targeted drug delivery ,Biotechnology & Applied Microbiology ,cytology ,cells ,nanoparticles ,Gold ,business ,Tomography, X-Ray Computed ,Neoplasm Transplantation ,Biomedical engineering - Abstract
Background Nanoparticles can be used for targeted drug delivery, in particular for brain cancer therapy. However, this requires a detailed analysis of nanoparticles from the associated microvasculature to the tumor, not easy because of the required high spatial resolution. The objective of this study is to demonstrate an experimental solution of this problem, based in vivo and post-mortem whole organ imaging plus nanoscale 3-dimensional (3D) X-ray microscopy. Results The use of gold nanoparticles (AuNPs) as contrast agents paved the way to a detailed high-resolution three dimensional (3D) X-ray and fluorescence imaging analysis of the relation between xenografted glioma cells and the tumor-induced angiogenic microvasculature. The images of the angiogenic microvessels revealed nanoparticle leakage. Complementary tests showed that after endocytotic internalization fluorescent AuNPs allow the visible-light detection of cells. Conclusions AuNP-loading of cells could be extended from the case presented here to other imaging techniques. In our study, they enabled us to (1) identify primary glioma cells at inoculation sites in mice brains; (2) follow the subsequent development of gliomas. (3) Detect the full details of the tumor-related microvasculature; (4) Finding leakage of AuNPs from the tumor-related vasculature, in contrast to no leakage from normal vasculature. Electronic supplementary material The online version of this article (doi:10.1186/s12951-015-0140-2) contains supplementary material, which is available to authorized users.
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- 2015
33. One-pot synthesis of AuPt alloyed nanoparticles by intense x-ray irradiation
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Bo-Jun Hsao, Giorgio Margaritondo, Ivan M. Kempson, Xiaoqing Cai, Chia-Chi Chien, Cheng-Liang Wang, Yeukuang Hwu, Hsiang-Hsin Chen, Yi-Yun Chen, Wen-Chang Chen, Sheng-Feng Lai, Wang, Cheng-Liang, Hsao, Bo-Jun, Lai, Sheng-Feng, Chen, Wen-Chang, Chen, Hsiang-Hsin, Chen, Yi-Yun, Chien, Chia-Chi, Cai, Xiaoqing, Kempson, Ivan M, Hwu, Y, and Margaritondo, G
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feed ratios ,Nanostructure ,Materials science ,Biocompatibility ,Mechanical Engineering ,technology, industry, and agriculture ,Nanoparticle ,Bioengineering ,macromolecular substances ,General Chemistry ,Polyethylene glycol ,equipment and supplies ,Colloid ,Crystallography ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,Mechanics of Materials ,Colloidal gold ,Transmission electron microscopy ,X-ray crystallography ,nanoparticles ,General Materials Science ,colloidal stability ,Electrical and Electronic Engineering - Abstract
We synthesized AuPt alloyed nanoparticles in colloidal solution by a one-pot procedure based on synchrotron x-ray irradiation in the presence of PEG (polyethylene glycol). The exclusive presence of alloyed nanoparticles with fcc structure was confirmed by several different experiments including UV–vis spectroscopy, x-ray diffraction (XRD) and transmission electron microscopy (TEM). The composition of the AuPt alloyed nanoparticles can be varied in a continuous fashion by simply varying the feed ratios of Au and Pt precursors. The nanoparticles exhibited colloidal stability and biocompatibility, important for potential applications. Refereed/Peer-reviewed
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- 2011
34. Tailored Au nanorods: optimizing functionality, controlling the aspect ratio and increasing biocompatibility
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Hsiang-Hsin Chen, Yi-Yun Chen, Yeukuang Hwu, Xiaoqing Cai, Cheng-Liang Wang, Sheng-Feng Lai, Giorgio Margaritondo, Chia-Chi Chien, Chung-Shi Yang, Tzu-En Hua, Ivan M. Kempson, Cai, Xianoqing, Wang, Cheng-Liang, Chen, Hsiang-Hsin, Chien, Chia-Chi, Lai, Sheng-Feng, Chen, Yi-Yun, Hua, Tzu-En, Kempson, Ivan M, Hwu, Y, Yang, C S, and Margaritondo, G
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fiber optic sensors ,Nanostructure ,Materials science ,Biocompatibility ,Reducing agent ,Dispersity ,Au nanorods ,Biocompatible Materials ,Bioengineering ,Nanotechnology ,Microscopy, Electron, Transmission ,Humans ,General Materials Science ,HeLa cells ,Irradiation ,metal nanoparticles ,Particle Size ,Electrical and Electronic Engineering ,Absorption (electromagnetic radiation) ,Nanotubes ,Aspect ratio (aeronautics) ,X-Rays ,Mechanical Engineering ,General Chemistry ,Mechanics of Materials ,gold nanoparticles ,Silver Nitrate ,Spectrophotometry, Ultraviolet ,Nanorod ,Gold ,HeLa Cells - Abstract
Monodisperse gold nanorods with high aspect ratio were synthesized by x-ray irradiation. Irradiation was first used to stimulate the creation of seeds. Afterward, nanorod growth was stimulated either by chemical reduction or again by x-ray irradiation. In the last case, the entire process took place without reducing agents. The shape of the final products could be controlled by modulating the intensity of the x-ray irradiation during the seed synthesis. In turn, the nanorod aspect ratio determines the absorption wavelength of the nanorods that can thus be optimized for different applications. Likewise, the aspect ratio influences the uptake of the nanorods by HeLa cells. Refereed/Peer-reviewed
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- 2010
35. Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy
- Author
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Hsiang-Hsin Chen, Yong S. Chu, Xiaoqing Cai, Cyril Petibois, Sheng-Feng Lai, Ivan M. Kempson, Yeukuang Hwu, Tzu-En Hua, Cheng-Liang Wang, Chia-Chi Chien, Giorgio Margaritondo, Yi-Yun Chen, Chen, Hsiang-Hsin, Chien, Chia-Chi, Petibois, Cyril, Wang, Cheng-Liang, Chu, Yong S, Lai, Sheng-Feng, Hua, Tzu-En, Chen, Yi-Yun, Cai, Xiaoqing, Kempson, Ivan M, Hwu, Yeukuang, and Margaritondo, Giorgio
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nanoparticle internalization ,Cytotoxicity ,Nanoparticle ,Metal Nanoparticles ,Medicine (miscellaneous) ,Pharmaceutical Science ,Apoptosis ,Cancer-Cells ,02 engineering and technology ,CIBM-PC ,Applied Microbiology and Biotechnology ,Polyethylene Glycols ,chemistry.chemical_compound ,Microscopy ,Gold Nanoparticles ,Microscopy, Phase-Contrast ,X-ray microscopy ,mammalian cells ,0303 health sciences ,Enhancement ,Magnetic Nanoparticles ,021001 nanoscience & nanotechnology ,Endocytosis ,lcsh:R855-855.5 ,Colloidal gold ,Drug delivery ,Nanomedicine ,Molecular Medicine ,0210 nano-technology ,Materials science ,lcsh:Medical technology ,Refractive-Index Radiology ,lcsh:Biotechnology ,Biomedical Engineering ,Nanotechnology ,Bioengineering ,Polyethylene glycol ,Cellular Uptake ,03 medical and health sciences ,Imaging, Three-Dimensional ,Microscopy, Electron, Transmission ,lcsh:TP248.13-248.65 ,Cell Line, Tumor ,Humans ,030304 developmental biology ,Staining and Labeling ,Research ,Drug-Delivery ,X-Ray Microtomography ,chemistry ,Magnetic nanoparticles ,Irradiation ,Gold ,Synchrotron-Radiation ,Quantitative analysis (chemistry) - Abstract
Background Quantitative analysis of nanoparticle uptake at the cellular level is critical to nanomedicine procedures. In particular, it is required for a realistic evaluation of their effects. Unfortunately, quantitative measurements of nanoparticle uptake still pose a formidable technical challenge. We present here a method to tackle this problem and analyze the number of metal nanoparticles present in different types of cells. The method relies on high-lateral-resolution (better than 30 nm) transmission x-ray microimages with both absorption contrast and phase contrast -- including two-dimensional (2D) projection images and three-dimensional (3D) tomographic reconstructions that directly show the nanoparticles. Results Practical tests were successfully conducted on bare and polyethylene glycol (PEG) coated gold nanoparticles obtained by x-ray irradiation. Using two different cell lines, EMT and HeLa, we obtained the number of nanoparticle clusters uptaken by each cell and the cluster size. Furthermore, the analysis revealed interesting differences between 2D and 3D cultured cells as well as between 2D and 3D data for the same 3D specimen. Conclusions We demonstrated the feasibility and effectiveness of our method, proving that it is accurate enough to measure the nanoparticle uptake differences between cells as well as the sizes of the formed nanoparticle clusters. The differences between 2D and 3D cultures and 2D and 3D images stress the importance of the 3D analysis which is made possible by our approach.
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36. Enhancement of irradiation effects on cancer cells by cross-linked dextran-coated iron oxide (CLIO) nanoparticles.
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Huang FK, Chen WC, Lai SF, Liu CJ, Wang CL, Wang CH, Chen HH, Hua TE, Cheng YY, Wu MK, Hwu Y, Yang CS, and Margaritondo G
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- Animals, Antineoplastic Agents administration & dosage, Biological Transport drug effects, Cell Line, Tumor, Cell Survival drug effects, Cell Survival radiation effects, Coculture Techniques, Dextrans administration & dosage, Dose-Response Relationship, Drug, Ferric Compounds administration & dosage, HeLa Cells, Humans, Metal Nanoparticles administration & dosage, Mice, Microscopy, Electron, Transmission, X-Ray Therapy methods, Antineoplastic Agents therapeutic use, Carcinoma radiotherapy, Carcinoma therapy, Dextrans therapeutic use, Ferric Compounds therapeutic use, Metal Nanoparticles therapeutic use
- Abstract
We investigated iron oxide nanoparticles with two different surface modifications, dextran coating and cross-linked dextran coating, showing that their different internalization affects their capability to enhance radiation damage to cancer cells. The internalization was monitored with an ultrahigh resolution transmission x-ray microscope (TXM), indicating that the differences in the particle surface charge play an essential role and dominate the particle-cell interaction. We found that dextran-coated iron oxide nanoparticles cannot be internalized by HeLa and EMT-6 cells without being functionalized with amino groups (the cross-linked dextran coating) that modify the surface potential from -18 mV to 13.4 mV. The amount of cross-linked dextran-coated iron oxide nanoparticles uptaken by cancer cells reached its maximum, 1.33 x 10(9) per HeLa cell, when the co-culture concentration was 40 microg Fe mL(-1) or more. Standard tests indicated that these internalized nanoparticles increased the damaging effects of x-ray irradiation, whereas they are by themselves biocompatible. These results could lead to interesting therapy applications; furthermore, iron oxide also produces high contrast for magnetic resonance imaging (MRI) in the diagnosis and therapy stages.
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- 2010
- Full Text
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