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Gold nanoparticles as multimodality imaging agents for brain gliomas
- Source :
- Journal of Nanobiotechnology
- Publication Year :
- 2015
- Publisher :
- UK : BioMed Central, 2015.
-
Abstract
- Background Nanoparticles can be used for targeted drug delivery, in particular for brain cancer therapy. However, this requires a detailed analysis of nanoparticles from the associated microvasculature to the tumor, not easy because of the required high spatial resolution. The objective of this study is to demonstrate an experimental solution of this problem, based in vivo and post-mortem whole organ imaging plus nanoscale 3-dimensional (3D) X-ray microscopy. Results The use of gold nanoparticles (AuNPs) as contrast agents paved the way to a detailed high-resolution three dimensional (3D) X-ray and fluorescence imaging analysis of the relation between xenografted glioma cells and the tumor-induced angiogenic microvasculature. The images of the angiogenic microvessels revealed nanoparticle leakage. Complementary tests showed that after endocytotic internalization fluorescent AuNPs allow the visible-light detection of cells. Conclusions AuNP-loading of cells could be extended from the case presented here to other imaging techniques. In our study, they enabled us to (1) identify primary glioma cells at inoculation sites in mice brains; (2) follow the subsequent development of gliomas. (3) Detect the full details of the tumor-related microvasculature; (4) Finding leakage of AuNPs from the tumor-related vasculature, in contrast to no leakage from normal vasculature. Electronic supplementary material The online version of this article (doi:10.1186/s12951-015-0140-2) contains supplementary material, which is available to authorized users.
- Subjects :
- fiber optic sensors
Fluorescence-lifetime imaging microscopy
Pharmaceutical Science
Medicine (miscellaneous)
Nanoparticle
Contrast Media
Metal Nanoparticles
02 engineering and technology
Applied Microbiology and Biotechnology
Mice
Microscopy
Medicine
metal nanoparticles
Internalization
media_common
0303 health sciences
Brain Neoplasms
Optical Imaging
Brain
Glioma
021001 nanoscience & nanotechnology
Endocytosis
3. Good health
Colloidal gold
Molecular Medicine
Science & Technology - Other Topics
fluorescence
0210 nano-technology
media_common.quotation_subject
Biomedical Engineering
Bioengineering
Nanotechnology
03 medical and health sciences
In vivo
Animals
Nanoscience & Nanotechnology
030304 developmental biology
business.industry
Research
gold
medicine.disease
imaging techniques
Targeted drug delivery
Biotechnology & Applied Microbiology
cytology
cells
nanoparticles
Gold
business
Tomography, X-Ray Computed
Neoplasm Transplantation
Biomedical engineering
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Nanobiotechnology
- Accession number :
- edsair.doi.dedup.....3ea538ad3f5dd821d31b5abcd80cc874