1. Aluminum in liver cells – the element species matters
- Author
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Holger Sieg, Anna Lena Ellermann, Agnès Burel, Albert Braeuning, Soizic Chevance, Birgitta Maria Kunz, Alfonso Lampen, Kevin Hogeveen, Linda Böhmert, Valérie Fessard, Fabienne Gauffre, Pégah Jalili, Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Biosit : biologie, santé, innovation technologique (SFR UMS CNRS 3480 - INSERM 018), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-13-IS10-0005, Agence Nationale de la Recherche, Deutsche Forschungsgemeinschaft, LA 3411/1-1, German Research Foundation DFG, ANR-13-IS10-0005,SolNanoTOX,Détermination de facteurs de toxicité au niveau intestinal et hépatique de deux nanoparticules de taille similaire utilisées en alimentation et en emballage : Recherches in vitro et in vivo sur l'absorption et les mécanismes impliqués.(2013), Laboratoire de Fougères, Bâtiment Bioagropolis, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)
- Subjects
HepG2 ,Cell Survival ,Biomedical Engineering ,Metal Nanoparticles ,Human metabolism ,Apoptosis ,[SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain ,02 engineering and technology ,010501 environmental sciences ,Toxicology ,medicine.disease_cause ,nanoparticule ,01 natural sciences ,Microscopy, Electron, Transmission ,Aluminum Oxide ,medicine ,Aluminum Chloride ,Humans ,0105 earth and related environmental sciences ,apoptose ,Chemistry ,nanoparticle ,aluminium ,digestive, oral, and skin physiology ,fungi ,toxicologie ,food and beverages ,Biological Transport ,Hep G2 Cells ,cell ,foie ,021001 nanoscience & nanotechnology ,Oxidative Stress ,Liver ,Biochemistry ,Nanoparticles ,cellule ,HepaRG ,0210 nano-technology ,Oxidative stress ,Aluminum ,DNA Damage - Abstract
International audience; Aluminum (Al) can be ingested from food and released from packaging and can reach key organs involved in human metabolism, including the liver via systemic distribution. Recent studies discuss the occurrence of chemically distinct Al-species and their interconversion by contact with biological fluids. These Al species can vary with regard to their intestinal uptake, systemic transport, and therefore could have species-specific effects on different organs and tissues. This work aims to assess the in vitro hepatotoxic hazard potential of three different relevant Al species: soluble AlCl3 and two nanoparticulate Al species were applied, representing for the first time an investigation of metallic nanoparticles besides to mineral bound gamma-Al2O3 on hepatic cell lines. To investigate the uptake and toxicological properties of the Al species, we used two different human hepatic cell lines: HepG2 and differentiated HepaRG cells. Cellular uptake was determined by different methods including light microscopy, transmission electron microscopy, side-scatter analysis, and elemental analysis. Oxidative stress, mitochondrial dysfunction, cell death mechanisms, and DNA damage were monitored as cellular parameters. While cellular uptake into hepatic cell lines occurred predominantly in the particle form, only ionic AlCl3 caused cellular effects. Since it is known, that Al species can convert one into another, and mechanisms including 'trojan-horse'-like uptake can lead to an Al accumulation in the cells. This could result in the slow release of Al ions, for which reason further hazard cannot be excluded. Therefore, individual investigation of the different Al species is necessary to assess the toxicological potential of Al particles.
- Published
- 2019