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Aluminum in liver cells – the element species matters
- Source :
- Nanotoxicology, Nanotoxicology, 2019, 13 (7), pp.909-922. ⟨10.1080/17435390.2019.1593542⟩, Nanotoxicology, Taylor & Francis, 2019, 13 (7), pp.909-922. ⟨10.1080/17435390.2019.1593542⟩
- Publication Year :
- 2019
- Publisher :
- Informa UK Limited, 2019.
-
Abstract
- International audience; Aluminum (Al) can be ingested from food and released from packaging and can reach key organs involved in human metabolism, including the liver via systemic distribution. Recent studies discuss the occurrence of chemically distinct Al-species and their interconversion by contact with biological fluids. These Al species can vary with regard to their intestinal uptake, systemic transport, and therefore could have species-specific effects on different organs and tissues. This work aims to assess the in vitro hepatotoxic hazard potential of three different relevant Al species: soluble AlCl3 and two nanoparticulate Al species were applied, representing for the first time an investigation of metallic nanoparticles besides to mineral bound gamma-Al2O3 on hepatic cell lines. To investigate the uptake and toxicological properties of the Al species, we used two different human hepatic cell lines: HepG2 and differentiated HepaRG cells. Cellular uptake was determined by different methods including light microscopy, transmission electron microscopy, side-scatter analysis, and elemental analysis. Oxidative stress, mitochondrial dysfunction, cell death mechanisms, and DNA damage were monitored as cellular parameters. While cellular uptake into hepatic cell lines occurred predominantly in the particle form, only ionic AlCl3 caused cellular effects. Since it is known, that Al species can convert one into another, and mechanisms including 'trojan-horse'-like uptake can lead to an Al accumulation in the cells. This could result in the slow release of Al ions, for which reason further hazard cannot be excluded. Therefore, individual investigation of the different Al species is necessary to assess the toxicological potential of Al particles.
- Subjects :
- HepG2
Cell Survival
Biomedical Engineering
Metal Nanoparticles
Human metabolism
Apoptosis
[SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain
02 engineering and technology
010501 environmental sciences
Toxicology
medicine.disease_cause
nanoparticule
01 natural sciences
Microscopy, Electron, Transmission
Aluminum Oxide
medicine
Aluminum Chloride
Humans
0105 earth and related environmental sciences
apoptose
Chemistry
nanoparticle
aluminium
digestive, oral, and skin physiology
fungi
toxicologie
food and beverages
Biological Transport
Hep G2 Cells
cell
foie
021001 nanoscience & nanotechnology
Oxidative Stress
Liver
Biochemistry
Nanoparticles
cellule
HepaRG
0210 nano-technology
Oxidative stress
Aluminum
DNA Damage
Subjects
Details
- ISSN :
- 17435404 and 17435390
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Nanotoxicology
- Accession number :
- edsair.doi.dedup.....bf3e9bfba886e0c63635097f9a4bb369