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Sulfonic acid groups with C3ν symmetry can coordinate with metal ions to produce multidimensional structures due to their flexible coordination modes. They can also penetrate into supramolecular structures through an intriguing bridging pattern and weak interactions. Herein, a novel heterocyclic sodium sulfonate supramolecular structure, namely [Na(Pyr-SO3)(H2O)]n where Pyr-SO3H is pyrazine sulfonic acid, is synthesized by utilizing NaBF4 to coordinate with the P–SO3H ligand through the solvent evaporation method. The single crystal X-ray diffraction (XRD) data indicate that the as-formed structure belongs to the Pbca space group. Additional properties are characterized by powder XRD, thermal analysis, and solid-state fluorescence. In particular, the introduction of a soft alkali metal ion can coordinate with the oxygen atom of the sulfonate ligand and form a Na–O bridging configuration that can not only significantly improve the pyrazine sulfonic acid ligand coordination ability, but also provide a reference for the extended study of functional sulfonate polymers in the future. Electronic supplementary material Supplementary material is available for this article at 10.1134/S0022476621110172 and is accessible for authorized users.

Background Renal hyperparathyroidism is a disease entity that is complex and poorly understood. Although there are guidelines regarding how to manage this patient group, evidence is scarce. Therefore, this survey-based study aims to map the physicians’ attitude in terms of preference for management of renal hyperparathyroidism and the influence of patient and respondent factors. Methods A survey was sent to Dutch societies of nephrology, endocrinology, and surgeons with interest in endocrine surgery. The survey consisted of eight case vignettes of renal hyperparathyroidism patients who were on hemodialysis and suitable for kidney transplantation, and varied in one of three patient variables import for decision making: age (40 vs. 65 years), parathyroid hormone (40 vs. 90 pmol/L), and serum calcium level (2.25 vs. 2.8 mmol/L). For each case, respondents could choose between maintaining conservative treatment (active vitamin D metabolites), calcimimetics, or subtotal parathyroidectomy as their treatment of choice. Categorical multilevel logistic models were used to investigate the association of patient and respondent variables with treatment preference. The influence of patient variables was determined independently of each other and by means of logistic regression the probabilities of treatment choice were calculated. Results In total, 115 surveys were included in the analysis. In 6 out of 8 cases, less than two-thirds of respondents agreed on the most favoured treatment. Among patient characteristics, the main disincentive for respondents not to choose conservative therapy was an elevated serum calcium level (subtotal parathyroidectomy vs conservative OR 93.1, 95%-CI: 48.39–179.07 and calcimimetics vs conservative OR 31.2 95%-CI: 18.58–52.30). Additionally, the most significant treatment differences were found between medical specialties and the experience of the respondents, expressed as the amount of cases the physician was involved in during the past year. Conclusions Elevated serum calcium levels were widely recognized and the prime reason for respondents to abandon conservative treatment. However, considerable disagreement in treatment preferences remained throughout the cases, demonstrating the current literature available being inconclusive in guiding physicians. Therefore, a high-quality trial comparing subtotal parathyroidectomy to medical treatment is needed to determine optimal treatment.

本文报道1例临床分期为cT(4a)N(2)M(0)的微卫星稳定的61岁男性胃体腺癌患者，术前给予免疫检查点抑制剂（PD-1单抗）联合XELOX（奥沙利铂+卡培他滨）化疗治疗3次后，行标准的全胃切除术+D(2)淋巴结清扫术，肿瘤达到病理完全缓解。随访9个月，未见复发。.

Systemic immune cell dynamics during coronavirus disease 2019 (COVID-19) are extensively documented, but these are less well studied in the (upper) respiratory tract, where severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates

The current study was performed to investigate the prevalence, associated risk factors exploration and phylogenetic analysis of Echinococcus granulosus (E. granulosus) genotypes isolated from Tibetan pigs. A total 373 Tibetan pigs were examined during 2014 and 2015, and the variables potentially associated with E. granulosus infection were explored with a multivariable logistic regression model. E. granulosus cysts (n=37) were collected from Tibetan pigs (lungs or livers). Fragments amplification of mitochondrial (mt) DNA of cox1 (shorter and longer) and atp6 were employed. The genotype of E. granulosus were identified by sequence and phylogenetic analysis. Results showed the prevalence of E. granulosus in Tibetan pigs was 9.9%. The prevalence of E. granulosus in male and female Tibetan pigs was 6.8% and 13.3%, with a significant difference in the two genders (P0.05). In different seasons, the infection rate of E. granulosus in Tibetan pigs were ranged from 5.8% to 12.3%. E. granulosus infection rates in different growing stages of Tibetan pigs were ranged from 4.4% to 15.9%, with a statistical difference in the three stages (P0.01). The prevalence of E. granulosus in Tibetan pigs were 7.9% in 2014 and 13.0% in 2015. Genders and growing stages were demonstrated to be risk factors to influence the prevalence significantly through multivariable logistic regression model. All the three fragments were successfully amplified from each of the 37 cysts. E. granulosus genotypes of G4 and G6 were identified by comparing with reference sequences of E. genotypes available at NCBI database and phylogenetic analysis by using MEGA software.

本例患者为肠癌术后局部复发，由于原手术粘连以及患者体质指数大（初诊为重度肥胖），预计手术难度较大。本例患者基于多学科诊疗模式进行治疗决策，并在术前通过三维重建技术，确认了肿瘤位于结肠系膜内，避免了无效探查；明确了肠系膜下动脉的走行以及肠系膜下静脉的引流区域，判断原手术清扫范围存在的不足。.

胎盘母体血管灌注不良（maternal vascular malperfusion，MVM）包括一系列在母体蜕膜血管中所表现的病理改变，其本质是妊娠过程中子宫螺旋动脉重塑异常导致的绒毛间隙氧合和血流动力学的异常。MVM在胎盘病理检查中是很容易被识别的一系列病理表现，但是国内病理医师对胎盘病理诊断的重视程度不够。该文就MVM的大体表现、镜下表现和临床意义做一介绍。.

胃肠道间质瘤（GIST）转移至浆膜腔者非常罕见。现就1例原发于胃的GIST且胸腔积液中查见GIST肿瘤细胞的病例进行分析，观察患者胸腔积液涂片的细胞学形态、细胞块及相应组织学切片的形态特点，总结GIST在浆膜腔积液中的细胞学诊断要点及鉴别诊断要点。.

Mutations in phosphatase and tensin homologue deleted on chromosome 10 (PTEN), a tumor suppressor gene, lead to altered sensitivity to drugs and radiation in various types of cancer. Restoring PTEN expression in tumor cells can increase radiosensitivity by inhibiting the repair of DNA double-strand breaks (DSBs). Thus, determining the mechanism of action of this protein may lead to novel therapeutic strategies.In this study, we transduced U251 cells with a lentiviral vector expressing PTEN to examine the mechanism of radiosensitization. Specifically, we examined the formation of radiation-induced DNA DSBs and apoptosis, as well as the expression of several proteins involved in repairing DSBs (p53, ataxia-telangiectasia mutated, DNA-dependent protein kinase C, Ku70-80).Our results showed that PTEN transduction sensitized U251 cells to X-rays, increasing the number of DSBs per cell and fraction of cells undergoing apoptosis. Additionally, the average size of γH2AX nuclear foci was increased following irradiation. These findings were accompanied by a PTEN-dependent irradiation-independent increase in p53 levels and decrease in phosphorylated Ku70/80 levels.Our results suggest that PTEN affects radiosensitivity by reducing DSB repair and by enhancing the p53 pathway, leading to increased apoptosis.

Summary The effective pressure law for the intrinsic formation factor of low permeability sandstones from the Ordos Basin (northwest of China) was studied experimentally by measuring the electrical resistivity of brine saturated rock samples while cycling the pore and confining pressures, pp and pc. We used a 100,000 ppm NaCl solution so that the results could be directly interpreted in terms of the intrinsic formation factor F. The Response Surface method was used to construct a quadratic function of pp and pc fitting the experimental data, from which the coefficient α of the effective pressure law, peff=pc – αpp, was determined. We found that the coefficient α generally had low values, mainly from 0.2 to 0.4, thus contradicting the theoretical prediction that α should be equal to 1 for scale-invariant properties, such as F, in linear elastic materials having a homogeneous solid matrix. Since the two underlying assumptions, linear elasticity and homogeneity of the solid matrix, are likely violated in reservoir rocks, we tried to assess their effects on α using simple analytical models, based on idealized geometries of the pore and solid phases. Analysis of these models suggests that non-linear elasticity associated with the formation of solid-solid contacts in the compressed rocks (e.g., during closure of rough cracks or crack-like pores along the grain boundaries), may be more effective in producing the low α values observed than inhomogeneity of the solid matrix.

OBJECTIVE: Epstein-Barr virus associated gastric cancer (EBVaGC) is different from the traditional gastric cancer (Epstein-Barr virus non-associated gastric cancer, EBVnGC), and has unique clinicopathological features. This study investigated the largest single center cancer series so as to establish the clinicopathological and molecular characteristics of EBVaGC in China. METHODS: A retrospective analysis was conducted on EBVaGC and EBVnGC patients diagnosed at Peking University Cancer Hospital from 2003 to 2018 by comparing their clinicopathological features and prognosis. The gastric cancer (GC) dataset of public database was analyzed to obtain differentially expressed genes. The expression of important genes and their association with prognosis of GC were verified in GC tissues from our hospital. RESULTS: In this study, 3 241 GC patients were included, and a total of 163 EBVaGC (5.0%) patients were identified. Compared with EBVnGC, EBVaGC was higher in male and younger patients, and positively associated with remnant GC, poorly differentiated adenocarcinoma, and mixed type GC. EBVaGC was inversely related to lymph node metastasis. The 5-year survival rate of EBVnGC and EBVaGC was 59.6% and 63.2% respectively (P

OBJECTIVE: To investigate the prevalence and related risk factors of laryngopharyngeal reflux in otolaryngology. METHOD: During January 2019 to March 2019, the inpatients in otolaryngology were investigated by the questionnaire of reflux symptom index scale and the laryngopharyngeal reflux related risk factors were analyzed. RESULT: Among the 227 patients, 33 patients with suspected LPR contained 19 patients(20.7%) of 92 patients in the laryngopharyngeal group, 10 patients(16.1%) of 62 patients in the nasal group, and 4 patients(5.5%) of 73 patients in the ear group. LPR prevalence in the laryngopharyngeal group was statistically different from that in the ear group(P < 0.05). Univariate logistic regression analysis showed that age, gender, smoking, drinking and BMI were risk factors of LPR(P < 0.01). Multivariate logistic regression analysis indicated that BMI was an independent risk factor of LPR(P < 0.01). CONCLUSION: LPR had a high prevalence rate in otolaryngology related diseases, and appropriate synergistic anti-LPR treatment could be carried out while treating otolaryngology related diseases.

In this paper, a test bench was built to study the thermal performance of three typical air collectors. Based on the test data, this paper conducted a comprehensive study on the physical and economic performance of the solar air heating systems including indoor temperature, solar energy guarantee rate, investment payback period and life cycle cost, and gave recommendations for their applications. The research showed that under the specified air flow rate range, the efficiency of the three typical collectors all reached above 45%. When the flow rate increased twice, the efficiencies of the V-groove collector, S-type spoiler collector, seams penetration collector were increased by 16%, 20%, 19%, respectively. This research recommended the ideal flow rate for solar air heating in China was 36 m3/(h.m2) ~ 54 m3/(h.m2). The simulation results in15 typical cities showed that the solar energy guarantee rate was generally above 20%, and in more than half of cities the solar energy guarantee rate reached 30% or above, with the highest in Lhasa, reached 62%. Economic analysis indicated more than 70% of the cities in northern China have a simple payback period of less than 6 years. The results indicated that solar air heating technology not only has a wide range of adaptability but also yield great financial benefits in northern China. This paper will have a significant impact on guiding the application of solar air heating in rural areas of China.

AIM To determine the optimal thresholds for assessing intervertebral segment stenosis through haemodynamic parameters of colour Doppler ultrasound compared with computed tomography (CT) angiography. MATERIALS AND METHODS Fifty-four patients diagnosed with intervertebral stenosis on colour Doppler imaging were included. Twenty patients with normal vertebral arteries constituted a control group. Peak systolic velocity at the intervertebral stenosis (PSVIV-S) and the intervertebral segment distal to the stenosis (PSVIV-D), end diastolic velocity at the intervertebral stenosis (EDVIV-S), and the intervertebral segment distal to the stenosis (EDVIV-D) were measured, and the ratios of PSVIV-S/PSVIV-D and EDVIV-S/EDVIV-D were calculated. Cut-off values for the diagnosis of

The origin of cancers is associated with etiology as well as therapeutics. Several studies reveal that malignancies in children can originate in utero. However, a diagnostic approach to distinguish between cancers initiated pre- or postnatally is absent. Here we identified a transcriptional factor FEV (fifth Ewing variant) that was expressed in fetal hematopoietic cells and became silent after birth. We characterized that FEV was essential for the self-renewal of hematopoietic stem cells (HSCs). We next found that FEV was expressed in most infant leukemia samples, but seldom in adult samples, in accord with the known prenatal origins of the former. We further determined the majority of pediatric acute lymphoid leukemia (ALL) and acute myeloid leukemia (AML) were FEV positive. Moreover, FEV knockdown markedly impaired the leukemia-propagating ability of leukemic stem cells. We therefore identified FEV is unique to fetal HSCs and stably expressed in leukemic cells of prenatal origin. It may also provide a tractable therapeutic target.

This research presents a numerical approach to address the moving load problem of functionally graded (FG) beams with rotational elastic edge constraints, in which the regularized Dirac-delta function is used to describe a time-dependent moving load source. The governing partial differential equations of the system, derived in accordance with the classical Euler–Bernoulli beam theory, are approximated by the discrete singular convolution (DSC) method. The resulting set of algebraic equations can then be solved by the Newmark-β integration scheme. Such a singular Dirac-delta formulation is also employed as the kernel function of the DSC method. In this work, the material properties of FG beams are assumed to be changed in the thickness direction. A convergence study is performed to validate the accuracy and reliability of the numerical results. In addition, the effects of moving load velocity and material distribution on the dynamic behavior of elastically restrained FG beams are also studied to serve as new benchmark solutions. By comparing with the available results in the existing literature, the present results show good agreement. More importantly, the major finding of this work indicates that the DSC regularized Dirac-delta approach is a good candidate for moving load problems, since the equally spaced grid system adopted in the DSC scheme can achieve a preferable representation of moving load sources.

To explore the role of microRNA-203 in laryngeal cancer and its underlying mechanism in regulating cell invasion and apoptosis.MicroRNA-203 expression in laryngeal cancer tissues and paracancerous tissues was detected by quantitative real time-polymerase chain reaction (qRT-PCR). The regulatory effects of microRNA-203 on the invasion and apoptosis of laryngeal cancer cells were detected by transwell assay and flow cytometry, respectively. Dual-Luciferase reporter gene assay was performed to access the binding condition of microRNA-203 and LASP1. Both mRNA and protein levels of LASP1 in laryngeal cancer cells were detected after transfection with microRNA-203 mimic or microRNA-203 inhibitor by qRT-PCR and Western blot, respectively. Rescue experiments were finally performed to detect whether microRNA-203 regulates laryngeal cancer development via targeting LASP1.MicroRNA-203 was lowly expressed in laryngeal cancer tissues and cell lines. MicroRNA-203 knockdown in Hep-2 cells can promote the invasion and inhibit the apoptosis of laryngeal cancer cells. Subsequently, LASP1 was predicted to be the target gene of microRNA-203, which was further verified by the Dual-Luciferase reporter gene assay. LASP1 expression was negatively regulated by microRNA-203. Furthermore, rescue experiments showed that the regulatory effects of microRNA-203 on the invasion and apoptosis of laryngeal cancer cells were reversed by LASP1.We showed that lowly expressed microRNA-203 could promote the invasion and inhibit apoptosis of laryngeal cancer cells via inhibiting LASP1.

The aim of this study was to explore whether Dl-3-n-butylphthalide (DBT) could protect blood-brain barrier (BBB) of mice with experimental cerebral infarction and the relevant mechanism.Adult male CD-1 mice were selected as the study objects. The permanent middle cerebral artery occlusion (MCAO) model was prepared by Longa's modified suture-occluded method. The mice were randomly divided into 3 groups: the sham operation group (Sham group), the cerebral infarction model group (CI group) and the DBT (120 mg/kg) intervention group (DBT group). Neurologic function deficits were evaluated by Longa's modified scoring method after 24 h of permanent MCAO. The wet and dry weight method was used for measuring water content in brain tissues. 2% 2,3,5-triphenyltetrazolium chloride (TTC) staining method was applied to determine the volume of cerebral infarction. Changes in the protein and messenger ribonucleic acid (mRNA) expression levels of matrix metallopeptidase 9 (MMP-9), claudin-5, vascular endothelial growth factor (VEGF), glial fibrillary acidic protein (GFAP), NF-E2 related factor 2 (Nrf-2) and heme oxygenase 1 (HO-1) in ischemic brain tissues were detected using immunohistochemistry, Western blotting and quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). Ultrastructure changes in BBBs were observed under an electron microscope.DBT improved the neurologic function deficits of mice and reduced the infarction volume of mice with cerebral infarction. DBT alleviated edema and decreased the permeability of BBBs of mice with cerebral infarction. DBT down-regulated the expression of MMP-9 and up-regulated the expression of claudin-5 in brain tissues of mice with cerebral infarction. DBT increased the expressions of VEGF and GFAP. DBT improved the ultrastructure in capillary endothelial cells of BBBs and increased the expressions of Nrf-2 and HO-1.DBT may protect BBB by activating the Nrf-2/HO-1 signaling pathway, thus achieving its protective effect on the brain.

The electrical conductivity of brine-saturated rock is predominantly dependent on the geometry and topology of the pore space. When a resistive second phase (e.g., air in the vadose zone and oil/gas in hydrocarbon reservoirs) displaces the brine, the geometry and topology of the pore space occupied by the electrically conductive phase are changed. We investigated the effect of these changes on the electrical conductivity of rock partially saturated with brine. We simulated drainage and imbibition as invasion and bond percolation processes, respectively, in pipe networks assumed to be perfectly water-wet. The simulations included the formation of a water film in the pipes invaded by the nonwetting fluid. During simulated drainage/imbibition, we measured the changes in resistivity index as well as a number of relevant microstructural parameters describing the portion of the pore space saturated with water. Except Euler topological number, all quantities considered here showed a significant level of “universality,” i.e., insensitivity to the type of lattice used (simple cubic, body-centered cubic, or face-centered cubic). Hence, the coordination number of the pore network appears to be a more effective measure of connectivity than Euler number. In general, the simulated resistivity index did not obey Archie's simple power law. In log-log scale, the resistivity index curves displayed a substantial downward or upward curvature depending on the presence or absence of a water film. Our network simulations compared relatively well with experimental data sets, which were obtained using experimental conditions and procedures consistent with the simulations. Finally, we verified that the connectivity/heterogeneity model proposed by Bernabe et al. (2011) could be extended to the partial brine saturation case when water films were not present.

Brain iron levels are significantly increased in Parkinson's disease (PD) and iron deposition is observed in the substantia nigra (SN) of PD patients. It is unclear whether iron overload is an initial cause of dopaminergic neuronal death or merely a byproduct that occurs in the SN of PD patients. In this study, ceruloplasmin knockout (CP-/-) mice and mice receiving an intracerebroventricular injection of ferric ammonium citrate (FAC) were selected as mouse models with high levels of brain iron. These mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) by intraperitoneal injection. Their behavior and the dopaminergic neuron damage to their substantia nigra pars compacta (SNpc) were assessed. These findings suggest that the injection of FAC or the absence of the CP gene may exacerbate both the observed apoptosis of TH-positive neurons and the behavioral symptoms of the MPTP-treated mice. The intracerebroventricular injection of deferoxamine (DFO) significantly alleviated the neuronal damage caused by MPTP in CP-/- mice. Furthermore, our findings suggest that the increased nigral iron content exacerbates the oxidative stress levels, promoting apoptosis through the Bcl-2/Bax pathway and the activated caspase-3 pathway in the brain. Therefore, iron overload in the brain exacerbates dopaminergic neuronal death in SNpc and leads to the onset of PD. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.

Objectives Previous studies have proposed various endoscopic scoring systems to assess disease severity of patients with chronic rhinosinusitis (CRS). However, those assessments have not been validated. This study was designed to assess the Modified Lund - Kennedy (MLK) and the discharge, inflammation, and polyps/oedema (DIP) endoscopic scoring systems in patients with CRS. Methods A prospective study including 144 patients who underwent functional endoscopic sinus surgery (FESS). All participants were asked to finish SNOT-22, Lund-Mackay CT score and endoscopic examination evaluations before surgery and at 6 months after surgery. Endoscopic examination videos were evaluated using 3 scoring systems by two blinded rhinologists. The scores were compared in terms of responsiveness, validity, reliability and correlation with other scores. Results The MLK and DIP endoscopic scoring systems showed high test-retest reliability and inter-rater reliability. All endoscopic scoring systems showed significant differences between the scores recorded at each time point (all P lower than 0.90 when compared with the Lund - Kennedy (LK) endoscopic scoring system and showed a statistically significant difference in discriminant validity between symptomless and symptomatic cases. The MLK and DIP systems showed high correlations with other subject assessments and no correlation with SNOT-22. Conclusion MLK and DIP exhibit substantial responsiveness, validity and reliability. MLK and DIP may be well suited for clinical and research use.

The healing variable and enhancement variable were first defined by the fatigue ductility, and then based on the relationship of the damage variable, the healing variable and the enhancement variable, a nonlinear fatigue damage-healing model was proposed for predicting the fatigue life of the healed copper film by laser shock peening (LSP). The nonlinear fatigue damage cumulative process was considered in the model for the original specimen without LSP under constant and variable amplitude loadings. The results showed that the proposed nonlinear fatigue damage-healing model can predict the residual fatigue life for the damaged copper film specimen well.

The genomic organisation of the seven cultivated Vigna species, V. unguiculata, V. subterranea, V. angularis, V. umbellata, V. radiata, V. mungo and V. aconitifolia, was determined using sequential combined PI and DAPI (CPD) staining and dual-colour fluorescence in situ hybridisation (FISH) with 5S and 45S rDNA probes. For phylogenetic analyses, comparative genomic in situ hybridisation (cGISH) onto somatic chromosomes and sequence analysis of the internal transcribed spacer (ITS) of 45S rDNA were used. Quantitative karyotypes were established using chromosome measurements, fluorochrome bands and rDNA FISH signals. All species had symmetrical karyotypes composed of only metacentric or metacentric and submetacentric chromosomes. Distinct heterochromatin differentiation was revealed by CPD staining and DAPI counterstaining after FISH. The rDNA sites among all species differed in their number, location and size. cGISH of V. umbellata genomic DNA to the chromosomes of all species produced strong signals in all centromeric regions of V. umbellata and V. angularis, weak signals in all pericentromeric regions of V. aconitifolia, and CPD-banded proximal regions of V. mungo var. mungo. Molecular phylogenetic trees showed that V. angularis and V. umbellata were the closest relatives, and V. mungo and V. aconitifolia were relatively closely related; these species formed a group that was separated from another group comprising V. radiata, V. unguiculata ssp. sesquipedalis and V. subterranea. This result was consistent with the phylogenetic relationships inferred from the heterochromatin and cGISH patterns; thus, fluorochrome banding and cGISH are efficient tools for the phylogenetic analysis of Vigna species.