1. Hepatic iron storage is related to body adiposity and hepaticinflammation
- Author
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Min Soo Kim, Jayong Chung, Chan Yoon Park, Kyung-Ok Koo, and Sung Nim Han
- Subjects
0301 basic medicine ,Duodenal iron transporter ,medicine.medical_specialty ,Hephaestin ,Calorie ,Mild calorie restriction ,Hepatic non-heme iron ,Endocrinology, Diabetes and Metabolism ,Ferroportin ,Calorie restriction ,Hepcidin ,Medicine (miscellaneous) ,White adipose tissue ,03 medical and health sciences ,Internal medicine ,Body adiposity ,Medicine ,Nutrition and Dietetics ,biology ,business.industry ,Research ,Iron deficiency ,medicine.disease ,Ferritin ,030104 developmental biology ,Endocrinology ,Iron absorption ,biology.protein ,business - Abstract
Background Obesity has been reported to be associated with iron deficiency. However, few studies have investigated iron status in low adiposity. To investigate whether body adiposity was associated with altered hepatic iron status, we compared liver iron levels and markers involved in inflammation and iron absorption in obese, control, and mildly calorie restricted mice. Methods Seven week old C57BL/6 mice were fed control (10% kcal fat, Control) or high fat (60% kcal fat, HFD) diets, or reduced amount of control diet to achieve 15% calorie restriction (CR) for 16 weeks. Hepatic non-heme iron content and ferritin protein level, and hematocrit and hemoglobin levels were determined to assess iron status. Hepatic expression of Mcp-1 and Tnf-α were measured as hepatic inflammatory markers. Hepatic hepcidin (Hamp) and Bmp6, and duodenal Dmt1, Dcyt1b, hephaestin (Heph) and ferroportin mRNA levels were measured as factors involved in regulation of iron absorption. Results Hepatic non-heme iron and ferritin protein levels were significantly higher in the CR group compared with the Control group, and significantly lower in the HFD group. These two iron status markers showed significantly negative correlations with the amount of white adipose tissue (r = -0.689 for hepatic non-heme iron and r = -0.740 for ferritin). Hepatic Mcp-1 and Tnf-α mRNA levels were significantly lower in the CR compared with the HFD (74 and 47% lower) and showed significantly negative correlations with hepatic non-heme iron levels (Mcp-1: r = -0.557, P
- Published
- 2017