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Hepatic iron storage is related to body adiposity and hepatic inflammation.
- Source :
- Nutrition & Metabolism; 2/13/2017, Vol. 14, p1-9, 9p, 2 Charts, 5 Graphs
- Publication Year :
- 2017
-
Abstract
- Background: Obesity has been reported to be associated with iron deficiency. However, few studies have investigated iron status in low adiposity. To investigate whether body adiposity was associated with altered hepatic iron status, we compared liver iron levels and markers involved in inflammation and iron absorption in obese, control, and mildly calorie restricted mice. Methods: Seven week old C57BL/6 mice were fed control (10% kcal fat, Control) or high fat (60% kcal fat, HFD) diets, or reduced amount of control diet to achieve 15% calorie restriction (CR) for 16 weeks. Hepatic non-heme iron content and ferritin protein level, and hematocrit and hemoglobin levels were determined to assess iron status. Hepatic expression of Mcp-1 and Tnf-α were measured as hepatic inflammatory markers. Hepatic hepcidin (Hamp) and Bmp6, and duodenal Dmt1, Dcyt1b, hephaestin (Heph) and ferroportin mRNA levels were measured as factors involved in regulation of iron absorption. Results: Hepatic non-heme iron and ferritin protein levels were significantly higher in the CR group compared with the Control group, and significantly lower in the HFD group. These two iron status markers showed significantly negative correlations with the amount of white adipose tissue (r = -0.689 for hepatic non-heme iron and r = -0.740 for ferritin). Hepatic Mcp-1 and Tnf-α mRNA levels were significantly lower in the CR compared with the HFD (74 and 47% lower) and showed significantly negative correlations with hepatic non-heme iron levels (Mcp-1: r = -0.557, P < 0.05; Tnf-α: r = -0.464, P < 0.05). Hepatic Hamp mRNA levels were lower in the HFD and higher in the CR groups compared with the Control group, which could be a response to maintain iron homeostasis. Duodenal Dcyt1b mRNA levels were higher in the CR group compared with the HFD group and duodenal Heph mRNA levels were higher in the CR group than the Control group. Conclusion: We showed that body adiposity was inversely correlated with liver iron status. Low inflammation levels in hepatic milieu and enhanced expression of duodenal oxidoreductases induced by calorie restriction could have contributed to higher iron status. [ABSTRACT FROM AUTHOR]
- Subjects :
- ADIPOSE tissue physiology
LIVER physiology
PROTEIN metabolism
ENZYME metabolism
ADIPOSE tissues
ANIMAL experimentation
BIOMARKERS
BIOLOGICAL transport
BODY composition
BODY weight
COMPARATIVE studies
STATISTICAL correlation
CYTOKINES
DIET in disease
DIET therapy
FERRITIN
FAT content of food
HEMATOCRIT
HEMOGLOBINS
HISTOLOGICAL techniques
INFLAMMATION
INGESTION
IRON
MICE
OBESITY
OXIDOREDUCTASES
POLYMERASE chain reaction
PROBABILITY theory
RESEARCH funding
STATISTICS
TUMOR necrosis factors
WESTERN immunoblotting
DATA analysis
STATISTICAL significance
DATA analysis software
DESCRIPTIVE statistics
NUTRITIONAL status
ONE-way analysis of variance
IN vivo studies
Subjects
Details
- Language :
- English
- ISSN :
- 17437075
- Volume :
- 14
- Database :
- Complementary Index
- Journal :
- Nutrition & Metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 121310986
- Full Text :
- https://doi.org/10.1186/s12986-017-0169-3