1. Effect of L-ethionine on macromolecular synthesis in mitogen-stimulated lymphocytes.
- Author
-
Zabos P, Kyner DA, Seide-Kehoe R, Acs G, and Christman JK
- Subjects
- Acetyltransferases metabolism, Adenosine Triphosphate metabolism, Animals, Dose-Response Relationship, Drug, Histones, Humans, Methyltransferases metabolism, Mice, Concanavalin A pharmacology, DNA biosynthesis, Ethionine pharmacology, Lymphocytes metabolism, Phytohemagglutinins pharmacology, Protein Biosynthesis, RNA biosynthesis
- Abstract
2--4 mM L-ethionine completely inhibits DNA synthesis in phytohaemagglutinin- or concanavalin A-stimulated lymphocytes even though it does not prevent the morphological changes characteristic of blast formation. Evidence is presented which indicates that complete commitment to DNA synthesis as well as a substantial increase in the rates of RNA and protein synthesis can occur in the presence of ethionine. Ethionine, however, does inhibit methylation of tRNA and prevents mitogen-induced increase in the activity of histone-modifying enzymes. All of these effects of exposure to ethionine are completely reversible. Removal of ethionine after 24 h or more of exposure results in a rapid, synchronous wave of DNA synthesis, an increase in the rate of methylation of RNA and an increase in activity of histone-modifying enzymes.
- Published
- 1978
- Full Text
- View/download PDF