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Effect of L-ethionine on macromolecular synthesis in mitogen-stimulated lymphocytes.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1978 Aug 23; Vol. 520 (1), pp. 139-52. - Publication Year :
- 1978
-
Abstract
- 2--4 mM L-ethionine completely inhibits DNA synthesis in phytohaemagglutinin- or concanavalin A-stimulated lymphocytes even though it does not prevent the morphological changes characteristic of blast formation. Evidence is presented which indicates that complete commitment to DNA synthesis as well as a substantial increase in the rates of RNA and protein synthesis can occur in the presence of ethionine. Ethionine, however, does inhibit methylation of tRNA and prevents mitogen-induced increase in the activity of histone-modifying enzymes. All of these effects of exposure to ethionine are completely reversible. Removal of ethionine after 24 h or more of exposure results in a rapid, synchronous wave of DNA synthesis, an increase in the rate of methylation of RNA and an increase in activity of histone-modifying enzymes.
- Subjects :
- Acetyltransferases metabolism
Adenosine Triphosphate metabolism
Animals
Dose-Response Relationship, Drug
Histones
Humans
Methyltransferases metabolism
Mice
Concanavalin A pharmacology
DNA biosynthesis
Ethionine pharmacology
Lymphocytes metabolism
Phytohemagglutinins pharmacology
Protein Biosynthesis
RNA biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 520
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 698226
- Full Text :
- https://doi.org/10.1016/0005-2787(78)90015-1