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Your search keyword '"Kojiro Maekawa"' showing total 2 results
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2 results on '"Kojiro Maekawa"'

1. Conformation-selective DNA strand breaks by dynemicin: a molecular wedge into flexible regions of DNA

Author

Tetsuya Kusakabe, Kojiro Maekawa, Atsushi Ichikawa, Motonari Uesugi, and Yukio Sugiura

Subjects
DNA -- Research, Antibiotics -- Analysis, Biological sciences, Chemistry
Abstract

Various oligonucleotides possessing a nickel structure or an extended base or a mismatched base were used to examine the conformational selectivity of dynemicin. The enediyne antibiotic dynemicin identifies and divides the areas of DNA that are conformationally pliant. Dynemicin could be a molecular wedge, which, by intercalating into the smaller-groove part of conformationally pliant areas of the DNA, attaches to the DNA.

Published
1993

2. Conformation-selective DNA strand breaks by dynemicin: a molecular wedge into flexible regions of DNA

Author

Motonari Uesugi, Yukio Sugiura, Kojiro Maekawa, Atsushi Ichikawa, and Tetsuya Kusakabe

Subjects
Dynemicin A, Antibiotics, Antineoplastic, Base Sequence, Stereochemistry, Intercalation (chemistry), Molecular Sequence Data, Anthraquinones, Biology, Biochemistry, Intercalating Agents, chemistry.chemical_compound, chemistry, Oligodeoxyribonucleotides, Enediyne, DNA unwinding, Weak association, Nucleic Acid Conformation, Enediynes, DNA, Minor groove, DNA Damage
Abstract

We present evidence that the enediyne antitumor antibiotic dynemicin recognizes and cleaves conformationally flexible regions of DNA. This is based on specific strand breaks at mismatches, bulges, and nicks as determined by high-resolution sequencing gels. On the basis of the weak association constant of dynemicin for DNA, it is expected that these more flexible regions would be preferred sites. In addition, the DNA unwinding behavior of dynemicin and the absorption spectrum of the dynemicin-DNA complex are strongly indicative of its intercalative binding with DNA. The results allow us to propose dynemicin as a molecular wedge that binds to the DNA by intercalating into the minor-groove side of conformationally flexible regions of DNA. Presumably, DNA local flexibility is able to create an open pocket in the minor groove, permits facile intercalation of dynemicin, and then increases the chances of its DNA damaging event. Implications for the biological action of dynemicin have also been discussed.

Published
1993

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