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High sugar consumption increases the chance of developing diabetes, hypertension, obesity and cancer, as well as cavities and bacterial plaque between teeth. Considering the increase in health problems related to sugar intake in the population, the objective of this work was to monitor the presence and amount of sugar in baby food based on fruits and vegetables from different companies sold in Brazil. Titrimetric methods (Lane-Eynon) and total sugar content were used, based on total soluble solids (TSS) in degrees Brix. In the titration method, it was possible to quantify reducing and non-reducing sugars and total sugar by the redox reaction of Fehling's liquor. Baby food with the highest levels of total sugars, presented 22.72% (assorted fruits) and 21.84% (plum base). The lowest total sugars content was 2.50% (from pumpkin and coconut milk composition). TSS analysis confirmed the presence of sugars found in the titration method. We concluded that the baby foods evaluated had relevant sugar content and there is a need for constant monitoring to avoid excesses by children, as well as careful attention to their nutritional information declared on the label, considering that high sugar consumption causes risks to the health.

Quase exclusivamente reconhecida como um alimento de alto valor calórico, a cerveja é constituída de numerosos bioativos capazes de promover benefícios à saúde auxiliando o tratamento de diversas doenças como as cardíacas e diabetes tipo II, salientando-se que seja consumida de maneira moderada. O presente trabalho teve por objetivo investigar o potencial de plantas amazônicas utilizadas na medicina tradicional como adjuntos no processo de produção de cerveja, afim de que o produto final tivesse as características medicinais da planta utilizada, para futuramente ser considerada uma “cerveja funcional” trazendo uma possível alternativa no mercado associado à incrementação de compostos com atividades biológicas da flora amazônica em seus produtos. Utilizaram-se plantas com uso consagrado pela etnofarmacologia e com estudos científicos que corroboram esta utilização. Foram preparadas através de processo cervejeiro tradicional utilizando como ingredientes maltes, lúpulo e levedura adquiridos no mercado nacional, 6 tipos de cervejas, sendo incrementadas dos seguintes aditivos: Arrabidaea chica (Crajiru); Genipa americana (Jenipapo); Chenopodium ambrosioides (Mastruz); e Caesalpinia Ferrea (Jucá). As cervejas foram avaliadas quanto ao seu teor de compostos fenólicos, utilizados como marcadores da atividade biológica pretendida e caracterizadas fisico- quimicamente de acordo com a legislação brasileira vigente. Os resultados obtidos foram promissores, com três das cervejas adicionadas de plantas apresentando maior concentração de compostos fenólicos que a cerveja sem adição de nenhuma delas. Além disso, todas as cervejas estiveram dentro dos limites legais de características físico-químicas e não apresentaram grande variação de parâmetros de acidez e pH da cerveja natural sem adição das espécies em estudo.

Bertholletia excelsa é uma espécie de florestas tropicais da família Lecythidacea. Fonte econômica primária para muitas populações extrativistas apresentando diversas características relevantes. Esta revisão tem como objetivo reunir artigos publicados nas bases de dados Periódico Capes, Pubmed, Science Direct e outros, utilizando descritores referentes a castanha do Brasil, sobre sua importância na saúde e economia. Foram encontrados 38387 artigos dos quais 107 foram avaliados subsequentemente com as leituras de título/resumos, destes 58 foram selecionados para nortear a revisão. Os resultados confirmam a importância do manejo econômico desta espécie nas comunidades tradicionais na cultura de coleta/beneficiamento, bem como suas propriedades biológicas as quais contribuem para a saúde da população. Estudos experimentais adicionais são necessários para a elucidação de mecanismos de ação, segurança e eficácia da espécie na tentativa do uso da planta como recurso biotecnológico.

Introduction: The hygienic and sanitary conditions of vessels carrying passengers and cargo requires constant diagnosis and monitoring for disease prevention. Objective: The objective of this study was to identify health hazards in food preparation conditions and water potability in mixed vessels in the State of Amazonas-Brazil. Method: 33 vessels were analyzed with qualitative detection analysis of Escherichia coli by chromogenic substrate for water potability and environmental evaluation with questionnaire application. Results: There was microbiological contamination in 13 (39.39%) drinking water samples and 31 (93.93%) sink samples for E. coli. The structural and operational conditions of kitchen handlers mostly did not meet the minimum percentages of safety in food production. Conclusions: This is the first report on the structural and operational conditions of food preparation in mixed vessels in Manaus-AM and we conclude that intervention is necessary with public policies regarding hygienic and sanitary issues involving the supply of water served in vessels for the protection of passenger health and training of food handlers and inspection of environments.

Aflatoxins (AFL) are fungal metabolites that can contaminate animal feed, causing toxic effects after consumption. Among the AFL, there are reports of the occurrence of aflatoxin M1 (AFM1) in milk, so the objective of this work was to evaluate the AFM1 content in bovine milk samples produced in the State of Amazonas-Brazil. Of the analyzed samples (n=30), 20% were found to be contaminated with AFM1 in the range of 0.093-0.320 μg/kg (Limit of detection= 0.0625 μg/mL). No sample exceeded current Brazilian legislation, but all exceeded the maximum European regulatory limit of 0.05 μg/kg. Risk analysis estimated a daily intake of 5.357 x 10-7 mg/kg body weight/day for men and 6.257 x 10-7 mg/kg body weight/day for women. The MOE result did not indicate concern in public health aspects. On the other hand, the occurrence of AFM1 in the state of Amazonas was observed in an unprecedented way and need further investigation including other dairy products.

The present study was a pilot study that aimed to evaluate the occurrence of aflatoxins (AF) in the human diets and its presence in human urine as a metabolite (aflatoxin M1; AFM1). Volunteers from the Amazon region were evaluated before and after the consumption of 2 Brazil nuts/day for thirty days. At the end of 30 days (t=30) without the consumption of Brazil nuts, 9 samples (30%) were positive for AFM1. After 30 days (t=30) consuming 2 Brazil nuts per day, there was a reduction to 2 positive samples (7%). Questionnaires were also applied to volunteers, and the foods most often cited as consumed in their normal diet were cereals and bovine milk. However, there was no statistical relationship between these foods and the levels of AFM1 in the urine or in relation to those who already consumed Brazil nuts in culinary preparations or derived products. Despite the reduction of positive samples for AFM1 after 30 days of Brazil nut consumption, we suggest further studies regarding the clinical condition and genetics of individuals from the Amazon region, as well as the nutrient levels of the diet, e.g. for selenium, and which may protect the human body against aflatoxins.

Milk is a beverage very susceptible to various contaminants, including aflatoxins. These toxins, especially aflatoxin B1 and M1, are considered potentially toxic to human and animal health and must be constantly controlled. For this reason, this systematic review aimed to evaluate the prevalence of aflatoxins in milk from the countries that are part of the Southern Common Market (Mercosur) to answer the research question: What is the prevalence of Aflatoxins in milk samples within the scope of Mercosur through studies published since 2010?”. The study was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, and the risk of bias was assessed using the Joanna Briggs Institute's tool for a critical assessment of adapted prevalence studies. A total of 7,698 articles were retrieved and 43 were included in the review, totaling 4,382 samples of milk analyzed and 177 of infant formula. AFM1 was the aflatoxin researched by all studies, and its overall prevalence at any concentration was 69% (IC95%: 55-80). Meta-analysis summarized concentrations for fluid milk of 0.0353 µg/kg (IC95%: 0.0187 – 0.0518), and for powdered milk of 0.4181 µg/kg (IC95%: 0.2301 – 0.6060). The results indicated a high prevalence of AFM1 in the member countries of Mercosur, but the calculated concentrations are under current legislation. This study is part of the protocol registered with the International Prospective of Systematic Reviews (PROSPERO), registration number: CRD42020200581.

Carcinogenic metabolites of fungi such as aflatoxins play a toxic role in some tree nuts and need to be monitored in their by-products, such as oil. In this context, Brazil nut (Bertholletia excelsa) oil, which is a commodity of great economic importance to Brazil, requires attention to monitor the presence of these toxic agents. Therefore, this study aimed to evaluate the presence of aflatoxins in Brazil nut oil and relate it to the presence of fatty acids in the oil as a surveillance tool for food safety. Brazil nut oil samples (n= 25) were acquired in northern Brazil as (a) non-branded products (n= 07) produced by local farmers using artisanal methods from nuts to be discarded by the industry and (b) industrialized products (n= 18). The samples were analyzed for total aflatoxin content by high-performance liquid chromatography and fatty acid content by nuclear magnetic resonance imaging. Seven (28%) samples were positive for the aflatoxin fractions (B1 + B2 + G1 + G2), ranging from undetected (2.32) to 50.87 μg/kg. Of the aflatoxin positive samples evaluated by NMR analysis, it was not possible to state that the presence of a particular fatty acid can interfere or influence aflatoxin contamination. This was the first study with data on aflatoxin occurrence in Brazil nut oil. Nevertheless, further research is required to relate saturated or unsaturated fatty acid content with aflatoxin levels. We also suggest the implementation of systems to prevent contamination of the raw materials (seed) and detoxification of the oil to guarantee the product's safety and quality.

Cassava flour is a Brazilian product that still deserves attention for what regards sustainability. We offer a study of production processes and nutritional aspects of the flour from the Central Brazilian Amazon. We conducted bibliometric surveys, observations and collected 30 samples, in 15 traditional communities, that need cassava as a source of income and livelihood. We found that cassava cultivation under specific edaphoclimatic conditions guarantees the maintenance of autochthonous landraces. Still, we show their influence upon the flour's physicochemical composition, especially starch, mineral and fiber contents. Further, nutritional analyses allowed a qualitative approach of human health. We propose recommendations, such as: the mitigation of effects of residual cyanide, classifying the flour as safe food; the maintenance of traditional communities, and of genetic resources. Still, we suggest the adoption of a seal of approval and origin, and the creation of a gourmet market, thus adding visibility to Brazil's intangible heritage variety.

Aflatoxins are the main mycotoxin related to the contamination of Brazil nut and an important product extracted from the Amazon region that has a high number of consumers due to its health benefits. Considering the frequent occurrence of aflatoxins in Brazil nuts, it is necessary to study the levels of exposure to these toxins associated with its consumption using biomarkers. To do this, a study was carried out with 30 volunteers of both sexes, where each volunteer received a kit containing Brazil nuts for the ingestion of 2 (two) units / day for 30 days. Urine samples were collected from each volunteer at 0 and 30 days, and the level of Aflatoxin M1 (AFM1) was determined by ELISA using the Helica Kit®. A questionnaire was used to obtain information on weight, height, age, medication use, pathological conditions, frequency of weekly consumption of certain foods more susceptible to aflatoxin contamination, and 24-h food recall. Of the 30 samples analyzed at time 0 (zero) for exposure, AFM1 presented positive results in 3 (10%) samples with a range of 2.75-70.41 ng/mL. In the analysis of the 30 days after Brazil nut consumption, the presence of AFM1 in the urine was not detected, suggesting that Brazil nut consumption did not generate impact on the urinary levels and exposure to AFM1. In conclusion, no association was found between food consumption of food susceptible to aflatoxin contamination and concentration of AFM1 in urine. Key words: Aflatoxin, nuts, ELISA, selenium.

Objective: Evaluate exposure to aflatoxins in processed Brazil nut (chopped and sliced) products marketed in Amazonas State. Methods: The samples were purchased during the 2017 harvest at the local retail in the city of Manaus/AM/Brazil in the form of sliced and chopped. Moisture content (MC) and water activity (aw) were verified, aflatoxins (AFB1, AFB2, AFG1, AFG2) were quantified by liquid chromatography. To characterize the risk of exposure to genotoxic use the population margin of exposure (MOE). Results: Chopped and sliced Brazil nut samples analyzed here presented an MC average of 1.62% and water activity of 0.26. These values indicate that samples are safe, according to physical-chemical baselines. Regarding aflatoxins presence, 8% of the samples presented aflatoxins total levels >10 μg/kg. A risk evaluation was performed in which exposure of the population to these substances is observed and, once found; the MOE was 1036 ± 793 (

Mycotoxins are secondary metabolites produced by several genera of fungi. Aspergillus sp. is responsible for producing the most relevant group nowadays, the Aflatoxins. The presence of aflatoxins in food can generate many economic problems for the producers and traders, but also for the health of consumers, as there is an association between consumption of contaminated food and risk of primary liver cancer. Peanut is among the most affected food by this mycotoxin and its analysis is important to safeguard the quality and economic value of the final product. Then, a Systematic Review study will be carried out to answer the research question “What is the prevalence of aflatoxins in peanuts in Mercosur countries since 2010?”. A systematic survey will be carried out in Medline electronic databases via PubMed, Embase, Web of Science, Scopus, LILACS via Bireme and SciELO.org; and in gray literature as Google Scholar, ProQuest, and the Brazilian Digital Library of Theses and Dissertations (BDTD). To carry out this study, the PRISMA guidelines (Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols) will be used. Thus, it is expected to verify: the prevalence of samples with concentrations above the maximum allowed limits for the regulation of Mercosur and the European Community; the average levels of aflatoxins in peanuts in Mercosur countries; the evolution of aflatoxin averages over time in the Mercosur region, since 2010; and estimate the average daily intake of aflatoxin by the peanut consumption in Mercosur countries. Keywords: Peanut; Aflatoxins; Mycotoxins; Mercosur; Systematic Review

Introduction\ud FoodEx2 consists of a vocabulary of foods with assigned codes, allowing the classification and description of foods reported in different types of data (food consumption, composition, or production method) [1]. At least 56 food consumption databases have been coded with FoodEx2 [2]. The linkage of greenhouse gas emissions (GHGE) databases to FoodEx2 codes would allow rapid matching of GHGE data to any food database previously harmonised with FoodEx2. We have linked 4558 FoodEx2 codes to GHGE data [3]. In this work, we aim to assess the reliability of the linkage, by comparing it to similar databases.\ud \ud Methods or approach\ud The main database (“City”) was developed by matching 43 food categories from Poore and Nemecek (2018) [4] to the 4558 FoodEx2 codes, adjusting for edibility. The items were matched by hand, using the closest raw product; if it was a product with multiple ingredients, we took the largest ingredient by weight. The reliability of the matching was assessed by comparing “City” to three GHGE databases: 1) SHARP (Mertens et al 2019) [5] has linked GHGE to FoodEx1 (an initial version of FoodEx); 2) Rose et al (2019) [6] and Heller et al (2018) [7] have GHGE data linked to National Health and Nutrition Examination Survey (NHANES), which is coded with FoodEx2; 3) Garzillo et al (2019) [8] have GHGE data linked to the Brazilian Food Consumption Survey. We have compared “City” with the three databases by calculating Spearman correlation coefficients. The food items with GHGE in both “City” and SHARP were ranked and split into quintiles. We checked quintile rankings of agreement between “City” and SHARP by calculating weighted kappa statistics. For food items not ranked in the same quintile, we checked whether values from SHARP were within the p5 and p95 from “City”.\ud \ud Findings and interpretations\ud The “City” dataset was strongly correlated to all three comparator databases. However, the number of directly comparable food items between all datasets were low. The number of food items compared, the Spearman correlation coefficients, and p-values are as follows: 1) “City” versus SHARP: n=945, r = 0.699, p < 0.001; 2) “City” versus Rose/Heller: n=608, r = 0.572, p < 0.001; 2) “City” versus Garzillo: n=329, r = 0.610, p < 0.001. Of the 945 food items with GHGE in “City” and SHARP, 50% (n = 476) were ranked in the same quintile. The kappa statistics was 0.536 (p < 0.001). Of the 469 food items not ranked into the same quintiles, 44% (n=206) were within p5 and p95, while 31% (n=144) were lower than the p5, and 25% (n= 119) were higher than p95. The food items with the biggest differences between “City” and SHARP are into the following food categories: wheat and rye; fish and seafood; pig meat; fruits; nuts and pulses. These food items will be further investigated in the next update of the data, aiming to increase reliability to estimate GHGE from food consumption.\ud \ud Conclusions\ud The FoodEx2 database linked to GHGE by City presented a strong correlation with other GHGE databases and therefore could be considered as a tool to estimate the environmental impacts from food. However, further work is still needed to refine the data, in particular checking values categorised in opposite quintiles and that do not fall within p5 and p95. This database allows for a quick link between GHGE and multiple dietary databases harmonised with FoodEx2.\ud \ud References\ud EFSA (European Food Safety Authority), Nikolic, M and Ioannidou, S, 2021. FoodEx2 maintenance 2020. EFSA supporting publication 2021: 18( 3):EN-6507. 19 pp. doi: 10.2903/sp.efsa.2020.EN-6507\ud Karageorgou, Dimitra and Lara-Castor, Laura and de Quadros, Victoria Padula and de Sousa, Rita Ferreira and Holmes, Bridget Anna and Ioannidou, Sofia and Mozaffarian, Dariush and Micha, Renata, Harmonizing Dietary Datasets for Global Surveillance: Methods and Findings From the Global Dietary Database. Under review.\ud Livestock, Environment And People (LEAP) Conference, Oxford, November 2019. C.J. Reynolds , X. Schmidt Rivera, , A. Frankowska, A Kluczkovski, J. T. da Silva S. L. Bridle,R. Levy, F. Rauber, V. P. Quadros, A. Balcerzak, R. F. Sousa, M. Ferrari, C. Leclercq, B. Koroušić Seljak, Tome Eftimov "A pilot method linking greenhouse gas emission databases to the FoodEx2 classification"\ud Poore, J., & Nemecek, T. (2018). Reducing food’s environmental impacts through producers and consumers. Science, 360(6392), 987–992.\ud Mertens, E., Kaptijn, G., Kuijsten, A., van Zanten, H., Geleijnse, J.M. and van't Veer, P., 2019. SHARP-Indicators Database towards a public database for environmental sustainability. Data in brief, 27, p.104617.https://doi.org/10.1016/j.dib.2019.104617\ud Rose D, Heller MC, Willits-Smith AM, Meyer RJ. "Carbon footprint of self-selected US diets: nutritional, demographic, and behavioral correlates," American Journal of Clinical Nutrition 2019;109:526-534. DOI:10.1093/ajcn/nqy327.\ud Heller MC, Willits-Smith A, Meyer R, Keoleian G, Rose D. "Greenhouse gas emissions and energy use associated with production of US self-selected diets," Environmental Research Letters 2018;13 044004. DOI: 10.1088/1748-9326/aab0ac.\ud Garzillo JMF, Machado PP, Costa Louzada ML, Levy RB, Monteiro CA. Footprints of foods and culinary preparations consumed in Brazil. https://doi.org/10.11606/9788588848405 (accessed Dec 7, 2020)

Bovine milk is a food consumed by people of different age groups and especially by children, as it provides essential nutrients to the diet. In Brazil, milk is one of the most important products of Brazilian agriculture, being essential in the generation of employment and income. However, when there is contamination by toxic agents, such as aflatoxin (AFL) which is carcinogenic to humans and animals, there is a risk to the health of the consumer. Therefore, the objective of this work was to analyze the efficiency of the application of UV LED light as a tool to reduce aflatoxin M1 (AFM1) in bovine milk produced in the state of Amazonas, Brazil. Concerning time in 3-, 6- and 9-minutes, the reduction rates of 75.5, 97.3, and 94.1% were observed, respectively. The use of UV LED light was effective in reducing the concentration of AFL studied to be evaluated on a production scale.

The Brazil nut is a nutritionally rich food, produced and consumed in tropical regions. On the other hand, it may be suitable to toxigenic fungi and consequently contamination by aflatoxins (AFL), toxic substances to consumers. In this context, the objective of this work was to evaluate the occurrence of AFL in raw Brazil nuts, that is, before being subjected to industrial drying stages. Of 23 samples were purchased at retail in the city of Manaus-Am-Brazil and evaluated by high performance and liquid chromatography for total AFL (B1+b2+G1+G2). From the samples, 8.6 % were positive for total AFL and. Only AFG1 and AFG2, were detected in the LOD (0.8 μg/kg) and met to the limit of

Cooking practices play a pivotal role in a healthy diet and lifestyle. Cooking is intertwined with dietary choices. To achieve a sustainable food system, we need to change how we cook and prepare food, along with the time we use to prepare and cook food. Cooking practices involve a variety of parameters such as cooking times, method of cooking (e.g. boiling, baking, steaming) and type of appliances (e.g. electric hobs, gas ovens, microwaves), which all influence the nutrition content and energy density of food and result in varying amounts of emitted greenhouse gases. Behavioural cooking choices are driven by factors such as convenience, taste, health and lifestyle and shape certain eating habits, but are also influenced by tradition and transfer of knowledge. This article presents the findings of three pilot surveys deployed in the United Kingdom, United States and Australia in 2019 (n = 385+ for each survey). These surveys asked about cooking and preparation time, methods and equipment for 30 common foods. Though based around similar food cultures, we find differences in cooking practices among the different countries’ populations. We then examine how food choices and cooking practices need to change differently in each country to be in line with current guidance on healthy, sustainable eating such as the guidelines provided by the EAT-Lancet report.

Aflatoxin M1 (AFM1) is a mycotoxin that can be found in cheeses and makes the monitoring of this food of public health interesting. In Brazil, the Amazon region has a cattle herd with dairy production of cheese, and an evaluation of the food and its risk to the population is required. The objective of this study was to evaluate the levels of AFM1, moisture content (mc) and water activity (aw) in different cheeses types, inspected or not by the food safety authority in the Amazonas State in Brazil. All samples of coalho-type cheese were found to be in compliance with mc and aw. Both mozzarella cheeses and Minas frescal cheeses presented average mc below the acceptable limits. As for the samples of mozzarella cheese, these presented aw according to the values acceptable by the legislation, however the average of the cheese Minas frescal was inferior to the recommended values. None of the 25 cheeses samples showed AFM1 contamination detectable by the HPLC method (LOQ = 0.0625 μg/mL). One possible explanation for the absence may be the lack of use of feed and confined animals, since in the state of Amazonas, for example, the milk-producing herd uses free grazing. Nevertheless, continuous monitoring is necessary since the consumption of cheeses produced in the state is relevant. Key words: Aspergillus, water activity, moisture content.

Introduction: The hygienic and sanitary conditions of vessels carrying passengers and cargo requires constant diagnosis and monitoring for disease prevention. Objective: The objective of this study was to identify health hazards in food preparation conditions and water potability in mixed vessels in the State of Amazonas-Brazil. Method: 33 vessels were analyzed with qualitative detection analysis of Escherichia coli by chromogenic substrate for water potability and environmental evaluation with questionnaire application. Results: There was microbiological contamination in 13 (39.39%) drinking water samples and 31 (93.93%) sink samples for E. coli. The structural and operational conditions of kitchen handlers mostly did not meet the minimum percentages of safety in food production. Conclusions: This is the first report on the structural and operational conditions of food preparation in mixed vessels in Manaus-AM and we conclude that intervention is necessary with public policies regarding hygienic and sanitary issues involving the supply of water served in vessels for the protection of passenger health and training of food handlers and inspection of environments. Introducción: la condición higiénico-sanitaria de los buques que transportan pasajeros y carga requiere un diagnóstico y seguimiento constantes para la prevención de enfermedades transmitidas por los alimentos (DTA). Objetivo: el objetivo de este trabajo era identificar los riesgos sanitarios en las condiciones de preparación de alimentos y la potabilidad del agua en buques mixtos en el estado de Amazonas-Brasil. Resultados: hubo contaminación microbiológica en 13 (39,39%) en muestras de agua potable y 31 (93,93%) de las muestras del fregadero a E. coli. Las condiciones estructurales y operativas de los manipuladores de cocina, en la mayoría no cumplían con los porcentajes mínimos de seguridad en la producción de alimentos. Conclusiones: este es el primer informe sobre las condiciones estructurales y operativas de la la preparación de alimentos en buques mixtos en Manaos-AM y llegamos a la conclusión de que es necesario intervenir con políticas públicas en materia de higiene-sanitarias relacionadas con el suministro de agua servida a los buques para proteger la salud de los pasajeros y la formación y el seguimiento de los manipuladores de alimentos. Introdução: A condição higiênico-sanitária de embarcações que transportam passageiros e cargas requer diagnóstico e monitoramento constantes para prevenção de doenças. Objetivo: Identificar perigos sanitários nas condições de preparação de alimentos e potabilidade de água em embarcações mistas em Manaus, Amazonas. Método: Foram analisadas 33 embarcações com análise de detecção qualitativa de Escherichia coli por substrato cromogênico para potabilidade da água e avaliação ambiental com aplicação de questionário. Resultados: Havia contaminação microbiológica em 13 (39,39%) amostras de água de bebedouro e 31 (93,93%) amostras de pia para E. coli. As condições estruturais e operacionais das embarcações em maioria não atendiam aos percentuais mínimos de segurança na produção de alimentos. Conclusões: Esse é o primeiro relato sobre as condições estruturais e operacionais da preparação de alimentos em embarcações mistas em Manaus e conclui-se que é necessária intervenção com políticas públicas quanto às questões higiênico-sanitárias que envolvam o fornecimento das águas servidas nas embarcações para proteção da saúde de passageiros, capacitação dos manipuladores de alimentos e inspeção de ambientes.