1. Formaldehyde effects on kanamycin resistance gene of inactivated recombinant Escherichia coli vaccines.
- Author
-
Donassolo RA, Ferreira MRA, Moreira C Jr, Dos Santos LM, Griep E, Moreira GMSG, Rodrigues RR, Moreira ÂN, and Conceição FR
- Subjects
- Escherichia coli drug effects, Escherichia coli Vaccines adverse effects, Escherichia coli Vaccines genetics, Gene Transfer, Horizontal drug effects, Plasmids genetics, Vaccines, Inactivated, Vaccines, Synthetic, Escherichia coli genetics, Formaldehyde pharmacology, Kanamycin Resistance drug effects, Plasmids drug effects
- Abstract
Objectives: Earlier studies have demonstrated the use of inactivated recombinant E. coli (bacterins), to protect against Clostridium spp. in vaccinated animals. These bacterins have a simpler, safer, and faster production process. However, these bacterins carry expression plasmids, containing antibiotic resistance gene, which could be assimilate accidentally by environmental microorganisms. Considering this, we aimed to impair this plasmids using formaldehyde at different concentrations., Results: This compound inactivated the highest density of cells in 24 h. KanR cassette amplification was found to be impaired with 0.8% for 24 h or 0.4% for 72 h. Upon electroporation, E. coli DH5α ultracompetent cells were unable to acquire the plasmids extracted from the bacterins after inactivation procedure. Formaldehyde-treated bacterins were incubated with other viable strains of E. coli, leading to no detectable gene transfer., Conclusions: We found that this compound is effective as an inactivation agent. Here we demonstrate the biosafety involving antibiotic resistance gene of recombinant E. coli vaccines allowing to industrial production and animal application.
- Published
- 2020
- Full Text
- View/download PDF