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Formaldehyde effects on kanamycin resistance gene of inactivated recombinant Escherichia coli vaccines.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2020 Nov; Vol. 42 (11), pp. 2223-2230. Date of Electronic Publication: 2020 Jun 04. - Publication Year :
- 2020
-
Abstract
- Objectives: Earlier studies have demonstrated the use of inactivated recombinant E. coli (bacterins), to protect against Clostridium spp. in vaccinated animals. These bacterins have a simpler, safer, and faster production process. However, these bacterins carry expression plasmids, containing antibiotic resistance gene, which could be assimilate accidentally by environmental microorganisms. Considering this, we aimed to impair this plasmids using formaldehyde at different concentrations.<br />Results: This compound inactivated the highest density of cells in 24 h. KanR cassette amplification was found to be impaired with 0.8% for 24 h or 0.4% for 72 h. Upon electroporation, E. coli DH5α ultracompetent cells were unable to acquire the plasmids extracted from the bacterins after inactivation procedure. Formaldehyde-treated bacterins were incubated with other viable strains of E. coli, leading to no detectable gene transfer.<br />Conclusions: We found that this compound is effective as an inactivation agent. Here we demonstrate the biosafety involving antibiotic resistance gene of recombinant E. coli vaccines allowing to industrial production and animal application.
- Subjects :
- Escherichia coli drug effects
Escherichia coli Vaccines adverse effects
Escherichia coli Vaccines genetics
Gene Transfer, Horizontal drug effects
Plasmids genetics
Vaccines, Inactivated
Vaccines, Synthetic
Escherichia coli genetics
Formaldehyde pharmacology
Kanamycin Resistance drug effects
Plasmids drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 42
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 32500473
- Full Text :
- https://doi.org/10.1007/s10529-020-02929-7