13 results on '"Kamran Abro"'
Search Results
2. Liquid Chromatographic Determination of Pioglitazone in Pharmaceuticals, Serum and Urine Samples
- Author
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Shahnaz Perveen, S.A. Mahesar, M.I. Bhanger, Najma Memon, and Kamran Abro
- Subjects
Pioglitazone ,Monolith column ,Pharmaceuticals ,Biological fluids. ,Environmental sciences ,GE1-350 ,Analytical chemistry ,QD71-142 - Abstract
A rapid and reliable analytical method based on high-performance liquid chromatography (HPLC) with UV detection (221nm) has been developed for the determination of the anti-hyperglycemic agent Pioglitazone in pharmaceutical formulations and biological fluids (serum and urine) after clean-up with solid-phase extraction. Chromatographic separation was achieved with a Chromolith® Performance RP-18e (100×4.6mm) column using mobile phase composition of acetonitrile: mixed phosphate buffer (pH 2.5; 10mM) (30:70, v/v) with a flow rate of 2.0mL/min. The total run time was 2 min. under optimized conditions. The calibration curve was found to be linear in the range of 1-10 µg mL-1 with regression coefficient of 0.9996, and the lower limit of detection 72 ng/20µL injection. The method has been validated for the system suitability, linearity, precision and accuracy, limits of detection, specificity, stability and robustness. The %recovery of Pioglitazone in pharmaceutical formulations was found to be 104.7%. The assay has been applied successfully to the pharmaceutical Tablet samples and biological fluids (serum and urine) of healthy volunteers.
- Published
- 2011
3. Sequential Microwave-Assisted Extraction of Oil from Layer Poultry Feeds and GC-MS Quantification of the Fatty Acids
- Author
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S. A. Mahesar, S. T. H. Sherazi, M. I. Bhanger, Kamran Abro, M. Younis Talpur, Aftab A. Kandhro, and Aijaz A. Bhutto
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Layer poultry feed oil ,Sequential microwave assisted extraction ,Soxhlet extraction ,Fatty acid composition ,trans Fat. ,Environmental sciences ,GE1-350 ,Analytical chemistry ,QD71-142 - Abstract
The present study reports the effect of sequential microwave-assisted extraction (SeMAE) on fatty acids composition (FAC) of layer poultry feed oil as compared to conventional Soxhlet extraction (SE) method. The FAC of extracted oil was determined by gas chromatography–mass spectrometry (GC-MS). There was no significant difference found in the amount of total extracted oil and FAC by SeMAE and SE. However, slightly greater content of trans fat in the samples revealed that SE lead to the formation of a little higher level of trans fat as compare to SeMAE. Therefore, the SeMAE could be used as a remarkable substitute to conventional SE for extraction of oil from the poultry feeds due to its faster speed, lesser solvent consumption, more environmental friendly.
- Published
- 2010
4. Simpler and Faster Spectrophotometric Determination of Diclofenac Sodium in Tablets, Serum and Urine Samples
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Abdul Rauf Khaskheli, Sirajuddin, Kamran Abro, S. T. H. Sherazi, H. I. Afridi, S. A. Mahesar, and Munawar Saeed
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Diclofenac sodium ,UV-Visible spectrophotometer ,tablets ,serum ,urine ,Environmental sciences ,GE1-350 ,Analytical chemistry ,QD71-142 - Abstract
This work describes a simple, sensitive, rapid and economical analytical procedure for direct spectrophotometric evaluation of diclofenac sodium (DS) using aqueous medium without using a chemical reagent. Parameters like time, temperature, acidic and basic conditions and interference by analgesic drugs were studied for a 5µg ml-1 solution of DS at 276 nm. Under optimized parameters, a linear working range of 0.1–30 g ml-1 with regression coefficient of 0.9998 and lower detection limit of 0.01 g ml-1 was obtained. The method was applied for DS contents in tablets, serum and urine samples.
- Published
- 2009
5. Efficacy Studies of Two Iron Supplements Irovit-1 and Irovit-2
- Author
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Atiq ur Rehman, Amir Ahmed, Rehana Jafri, Kamran Abro, and Irshad Ahmed Khan
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0106 biological sciences ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Population ,General Physics and Astronomy ,Iron-saccharate ,Hematocrit ,Hemoglobin levels ,01 natural sciences ,Elemental iron ,Animal science ,Red Blood Cell Count ,Oral supplements ,medicine ,General Materials Science ,Hemoglobin ,Physical and Theoretical Chemistry ,business ,education ,Mathematical Physics ,010606 plant biology & botany - Abstract
Two preparations of iron supplements have been developed with vitamins, Irovit-1 and Irovit-2 containing 1 mg/mL and 2 mg/mL elemental iron respectively in complex form. Clinical trials of Irovit-1 and Irovit-2 have been conducted randomly on selected anemic males and females from urban population of Karachi, an improvement in hemoglobin levels were estimated for the treatments with Irovit-1 (10 mg Fe/day) and Irovit-2 (20 mg Fe/day). Both preparations have been found to be effective in increasing hemoglobin, Hematocrit and red blood cell count. The mean hemoglobin levels were observed increased 0.73 ± 0.25 g/dL and 1.10 ± 0.30 g/dL, after 5 weeks by oral intake of Irovit-1 and Irovit-2, respectively.
- Published
- 2021
6. Recent Progress in the Analysis of Captopril Using Electrochemical Methods: A Review
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Syed Tufail Hussain Sherazi, Hamid Ali Kazi, Andrei A. Bunaciu, Saeed Ahmed Lakho, Sarfaraz Ahmed Mahesar, Razium Ali Soomro, and Kamran Abro
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Chemistry ,010401 analytical chemistry ,medicine ,Captopril ,02 engineering and technology ,021001 nanoscience & nanotechnology ,0210 nano-technology ,Electrochemistry ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Nuclear chemistry ,medicine.drug - Abstract
Background: Captopril is the synthetic dipeptide used as an angiotensin converting enzyme inhibitor. Captopril is used to treat hypertension as well as for the treatment of moderate heart failure. Analytical instrumentation and methodology plays an important role in pharmaceutical analysis. Methods: This review presents some important applications of electrochemical modes used for the analysis of captopril. So far captopril has been analyzed by using different bare and modified working electrodes with a variety of modifiers from organic and inorganic materials to various types of nano particles/materials. Results: This paper presents some of the methods which have been published in the last few years i.e. from 2003 to 2016. This review highlights the role of the analytical instrumentation, particularly electrochemical methods in assessing captopril using various working electrodes. Conclusion: A large number of studies on voltammetry noted by means of various bare and modified electrodes. Among all of the published voltammetric methods, DPV, SWV, CV and miscellaneous modes were trendy techniques used to analyze captopril in pharmaceutical formulations as well as biological samples. Electrodes modified with nanomaterials are promising sensing tools as this showed high sensitivity, good accuracy with precision as well as selectivity. In comparison to chromatographic methods, the main advantages of electrochemical methods are its cheaper instrumentation, lower detection limit and minimal or no sample preparation.
- Published
- 2019
7. Quantification of malachite green in fish feed utilising liquid chromatography-tandem mass spectrometry with a monolithic column
- Author
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Kamran Abro, Shahnaz Perveen, Sarfaraz Ahmed Mahesar, and Seema Iqbal
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Spectrometry, Mass, Electrospray Ionization ,Monolithic HPLC column ,Calibration curve ,Health, Toxicology and Mutagenesis ,Liquid-Liquid Extraction ,Antiprotozoal Agents ,Analytical chemistry ,Food Contamination ,Aquaculture ,Toxicology ,chemistry.chemical_compound ,Limit of Detection ,Tandem Mass Spectrometry ,Liquid–liquid extraction ,Liquid chromatography–mass spectrometry ,Rosaniline Dyes ,Animals ,Pakistan ,Ultrasonics ,Malachite green ,Chromatography, High Pressure Liquid ,Chromatography ,Selected reaction monitoring ,Fishes ,Pesticide Residues ,Public Health, Environmental and Occupational Health ,Reproducibility of Results ,General Chemistry ,General Medicine ,Food Inspection ,Animal Feed ,Capacity factor ,Fungicides, Industrial ,Teratogens ,chemistry ,Theoretical plate ,Mutagens ,Food Science - Abstract
The purpose of this study was to develop a rapid and sensitive method for the quantification of malachite green (MG) in fish feed using LC-ESI-MS/MS with a monolithic column as stationary phase. Fish feed was cleaned using ultrasonic assisted liquid-liquid extraction. The separation was achieved on a Chromolith® Performance RP-18e column (100 × 4.6 mm) using gradient mobile phase composition of methanol and 0.1% formic acid at the flow rate of 1.0 ml min⁻¹. The analyte was ionised using electrospray ionisation in positive mode. Mass spectral transitions were recorded in selected reaction monitoring (SRM) mode at m/z 329.78 → m/z 314.75 with a collision energy (CE) of 52% for MG. The system suitability responses were calculated for reproducibility tests of the retention time, number of theoretical plates and capacity factor. System validation was evaluated for precision, specificity and linearity of MG. The linearity and calibration graph was plotted in the range of 15.0-250 ng ml⁻¹ with the regression coefficient of0.997. The lower limits of detection and quantification for MG were 0.55 and 1.44 ng ml⁻¹, respectively, allowing easy determination in fish feed with accuracy evaluated as a percentage recovery of 92.1% and precision determined as % CV of5. The method was also extended to the determination of MG in an actual fish feed. The sensitivity and selectivity of LC-ESI-MS/MS using monolithic column offers a valuable alternative to the methodologies currently employed for the quantification of MG in fish feeds.
- Published
- 2014
8. Determination of Vitamins E, D3, and K1 in Plasma by Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Mass Spectrometry Utilizing a Monolithic Column
- Author
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Najma Memon, Kamran Abro, Suhail Abro, Abdul Hafeez Laghari, Muhammad Iqbal Bhanger, and Shahnaz Perveen
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Chemical ionization ,Monolithic HPLC column ,Chromatography ,Resolution (mass spectrometry) ,Chemistry ,Biochemistry (medical) ,Clinical Biochemistry ,Analytical chemistry ,Atmospheric-pressure chemical ionization ,Mass spectrometry ,Biochemistry ,Analytical Chemistry ,Electrochemistry ,Mass spectrum ,Solid phase extraction ,Spectroscopy ,Vitamin E Acetate - Abstract
This paper reports a rapid, simple, and sensitive method for determination of vitamin D3, vitamin E acetate, and vitamin K1 in plasma using atmospheric pressure chemical ionization –high performance liquid chromatography–mass spectrometry. Plasma samples were prepared using solid phase extraction. The separation of compounds was achieved using a C18 monolithic column and a mobile phase composed of methanol and 0.1% formic acid in gradient elution mode at a flow rate of 1.0 mL min−1. Analytes were ionized using atmospheric chemical ionization in positive mode. Mass spectra were recorded at m/z = 385.23, 473.47, and 451.41 for vitamin D3, vitamin E, and vitamin K1, respectively. Vitamin D2 was used as an internal standard and its mass spectra was recorded at 397.28 m/z. The method was validated using ICH guidelines. The system suitability responses were calculated for retention time, number of theoretical plates, capacity factor, resolution, and the selectivity factor. System validation was evaluated for pr...
- Published
- 2013
9. Comparative Study of Electrospray and Atmospheric Pressure Chemical Ionization with Liquid Chromatography–Mass Spectrometry for Quantification of Five Antihyperglycemic Agents Utilizing Monolithic Column
- Author
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Kamran Abro, Najma Memon, Shahnaz Perveen, Aijaz Panhwar, and Muhammad Iqbal Bhanger
- Subjects
Detection limit ,Electrospray ,Chromatography ,Monolithic HPLC column ,Chemistry ,Electrospray ionization ,Biochemistry (medical) ,Clinical Biochemistry ,Atmospheric-pressure chemical ionization ,Mass spectrometry ,Biochemistry ,Analytical Chemistry ,Liquid chromatography–mass spectrometry ,Electrochemistry ,Direct electron ionization liquid chromatography–mass spectrometry interface ,Spectroscopy - Abstract
Liquid chromatography-mass spectrometry (LC-MS) in atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI) modes were studied for a multi-component plasma and urine quantification of 5 antihyperglycemic agents (metformin, pioglitazone, gliclazide, glibenclamide, and glimperide). The separation of the compounds was achieved using Chromolith Performance RP-18e column (100 × 4.6 mm), with gradient mobile phase composition of acetonitrile −0.1% formic acid. MS parameters for APCI and ESI were optimized individually and were operated in positive mode. The detection limits for the metformin, pioglitazone, glibenclamide, and glimepiride were determined to be 6.84, 6.22, 13.03, and 44.38 ng mL−1 using LC-ESI-MS; and for LC-APCI-MS, it was determined to be 48.39, 8.02, 17.02, and 144.55 ng mL−1, respectively. Gliclazide was the only exception as it exhibited a lower limit of detection (LOD) using APCI than ESI which was found to be 5.61 and 23.43 ng mL−1, respectively. The method was vali...
- Published
- 2012
10. HPLC Determination of Betamethasone and Prednisolone in Urine Samples Using Monolithic Column
- Author
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Kamran Abro, Najma Memon, Rehana Jafri, Muhammad Iqbal Bhanger, and Shahnaz Perveen
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Monolithic HPLC column ,Chromatography ,Chemistry ,medicine ,Prednisolone ,General Physics and Astronomy ,Betamethasone ,General Materials Science ,Urine ,Physical and Theoretical Chemistry ,High-performance liquid chromatography ,Mathematical Physics ,medicine.drug - Published
- 2011
11. Application of microwave heating for the fast extraction of fat content from the poultry feeds
- Author
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Aftab Ahmed Kandhro, Jacqueline Sedman, Syed Tufail Hussain Sherazi, Kamran Abro, Sarfaraz Ahmed Mahesar, Muhammad Iqbal Bhanger, and F.R. van de Voort
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Time Factors ,Chromatography ,Microwave oven ,Extraction (chemistry) ,Fatty Acids, Nonesterified ,Animal Feed ,Chemistry Techniques, Analytical ,Poultry ,Analytical Chemistry ,Heating ,Solvent ,Hexane ,chemistry.chemical_compound ,chemistry ,Oil content ,Microwave heating ,Spectroscopy, Fourier Transform Infrared ,Solvents ,Animals ,Sample preparation ,Microwaves ,Microwave - Abstract
A rapid method has been developed to extract and quantitatively measure the total oil content in poultry feeds using a domestic microwave oven. The optimized extraction procedure involves the replicate (6×) extraction of 5 g of ground feed with 12 ml of hexane for 20 s in a 900 W oven. Each replicate involves the collection of the resulting miscella and its replacement with fresh solvent for re-extraction. The collected extracts were centrifuged and transferred to a vial. The solvent was evaporated to a constant weight and the residual lipid weighed. In comparison to conventional Soxhlet extraction method, lipid contents obtained using the optimized microwave procedure was not significantly different. However, FTIR analysis indicated that the microwave procedure was superior in minimizing the formation of free fatty acids (FFA) relative to the Soxhlet procedure if the temperature of the sample was kept within the range of 45–50 °C. This simple, sequential extraction procedure is rapid, highly efficient and provides a simple mean of quantitating the lipid content of poultry feed in less than 40 min without the need for specialized microwave oven.
- Published
- 2008
12. LC/UV determination of cefradine, cefuroxime, and cefotaxime in dairy milk, human serum and wastewater samples
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Tahira Qureshi, Najma Memon, Saima Q. Memon, S. W. Shah, and Kamran Abro
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Pharmaceutically active compounds ,Multidisciplinary ,Cefotaxime ,Chromatography ,business.industry ,Formic acid ,medicine.drug_class ,Elution ,Research ,Cephalosporin ,High-performance liquid chromatography ,Hospital wastewater ,Microbiology ,Cephalosporins ,chemistry.chemical_compound ,Milk ,chemistry ,Wastewater ,Cefradine ,Antibiotics ,Medicine ,business ,Cefuroxime ,medicine.drug ,High performance liquid chromatography - Abstract
Cephalosporins type antibiotics are widely used to treat infectious diseases. Their determination is not only important in blood/serum of patients under treatment but also in diverse matrices like wastewaters, milk etc. as contaminant. Keeping in view the need, a new high performance liquid chromatographic method for the determination of three cephalosporins (cefradine, cefuroxime and cefotaxime) has been developed. Separation was performed on an ODS column with binary solvent elution of aqueous formic acid (0.05%) and methanol in the ratio of 45: 55 (v/v) at a flow rate of 1 mL min-1 and UV detection at 260 nm. Under optimised conditions, all three cephalosporins were baseline separated within 5 min. Linear responses for cefradine 5–20 μg mL-1, cefuroxime 0.5-15 μg mL-1 and cefotaxime 1.0-20 μg mL-1 were established. LOD of 0.05-0.25 μg mL-1 after preconcentration was achieved. The method was applied to serum samples of patients under treatment with these antibiotics and to screen the selected cephalosporins from hospital wastewater and milk samples. Moreover, method was applied to study stability of aqueous solutions and acid/base induced degradation of all three drugs.
- Published
- 2013
13. Multi-component quantitation of loratadine, pseudoephedrine and paracetamol in plasma and pharmaceutical formulations with liquid chromatography-tandem mass spectrometry utilizing a monolithic column
- Author
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Najma Memon, Shahnaz Perveen, Aftab Ahmed Kandhro, Kamran Abro, and Muhammad Iqbal Bhanger
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Detection limit ,antihistamines ,Monolithic HPLC column ,Chromatography ,Chemistry ,Formic acid ,liquid chromatography-tandem MS ,Analytical chemistry ,General Chemistry ,Plasma ,Loratadine ,Pseudoephedrine ,Mass spectrometry ,lcsh:Chemistry ,chemistry.chemical_compound ,lcsh:QD1-999 ,decongestants ,Liquid chromatography–mass spectrometry ,medicine ,medicine.drug - Abstract
The purpose of this study was to develop a rapid, simple and sensitive quantitation method for pseudoephedrine (PSE), paracetamol (PAR) and loratadine (LOR) in plasma and pharmaceuticals using liquid chromatography-tandem mass spectrometry with a monolithic column. Separation was achieved using a gradient composition of methanol-0.1% formic acid at a flow rate of 1.0 mL min-1. Mass spectral transitions were recorded in SRM mode. System validation was evaluated for precision, specificity and linearity. Limit of detection for pseudoephedrine, paracetamol, and loratadine were determined to be 3.14, 1.86 and 1.44 ng mL-1, respectively, allowing easy determination in plasma with % recovery of 93.12 to 101.56%.
- Published
- 2012
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